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Document 1444066
Basic Biochemistry II Course Schedule: Jan – May 2013
Birla Institute of Technology and Science, Pilani
and
Elite School of Optometry
(Unit of Medical Research Foundation, Chennai)
B S OPTOMETRY
First Year - Second Semester – Academic Year (2012 – 2013)
COURSE HANDOUT (PART - II)
COURSE NO.
:
OPTO ZC162
COURSE TITLE
:
BASIC BIOCHEMISTRY II
INSTRUCTOR IN CHARGE
:
Ms R. Selvi
1. SCOPE & OBJECTIVES :
The course aims at making the students learn the basic biochemistry with
emphasis on fundamentals. It includes topics on organic aspects of (i) carbohydrates, proteins, lipids &
nucleopreoteins, (ii) enzymatology (iii) hormones and (iv) techniques. As life is nothing but metabolism,
i.e., reactions taking place in a living system, students will learn the main aspects of metabolism of major
biochemicals in the body, i.e. carbohydrates, proteins and lipids. They would also learn the physicochemical aspects of Biochemistry, techniques like electrophoresis including those using Radio isotopes
in analysis. Being students of Optometry, they would learn the basic facts in ocular structures and
their functions as also Clinical Biochemistry with reference to analysis of glucose, urea, creatinine and
bilirubin in blood and basic instrumentations.
2. TEXT BOOKS :
(a) S. Ramakrishnan: Essentials of Biochemistry & Ocular Biochemistry 1992, Publication Division,
Annamalai University. (EBO).
(b) G. Rajagopal & S. Ramakrishnan: Practical Biochemistry for Medical students, M/s. Orient Longman,
Calcutta, 1985. (For Practical).
(c) S. Ramakrishnan, K.G. Prasannam and R. Rajan: Text book of Medical Biochemistry 3rd Ed., 2001,
M/s. Orient Longman, Chennai. (TMB)
(d) Ramakrishna’s Biochemistry students manual, 1993.
3. REFERENCE BOOKS
:
(a) Varley H, Gowenlock A H, and Bell M : Practical Clinical Biochemistry, 5th Ed., William Heinemann
Medical Books Ltd., London 1980. (PCB)
(b) R E Anderson :Biochemistry of the Eye,American Academy of Ophthalmology, 1983, (BCE)
Basic Biochemistry II Course Schedule: Jan – May 2013
BASIC BIOCHEMISTRY I (THEORY)
3. COURSE PLAN : - ll – Ms R Selvi
S
Topics
NO
1 METABOLISM
Carbohydrates
Proteins
Lipids
2
HORMONES
Definition, function, examples.
No of
Lectures
2
2
2
2
2
2
III.OCULAR BIOCHEMISTRY
2
Structures
2
Functions
4
IV.TECHNIQUES AND PHYSICAL ASPECTS
2
Electrophoresis
2
Chromatography
2
Photometry
Radioisotope work
Solutions, pH, buffers.
2
Colloids, emulsions, sols, gels
2
5
CLINICAL BIOCHEMISTRY : BLOOD ANALYSIS
2
Glucose
2
Urea
Creatinine
Bilirubin
Total
30
4. READING ASSIGNMENTS
------------------------------------------------------------------------------------------------------SL. NO.
TOPICS
CHAPTER
PAGE NOS.
------------------------------------------------------------------------------------------------------1.
Hormones
(TMB)
358 – 359
2.
Metabolism
176 - 294
3.
Ocular Biochemistry
4.
Techniques and physical aspects
465 - 475
--------------------------------------------------------------------------------------------------------------------------------------------Evaluation Component Schedule:
Component Month
Pattern
Marks
3
EC – I and III
Feb & April
EC – II
March
Comp. Exam May
Assignment, quiz, viva, seminar, debate, lecture evaluation,
Surprise test continuous class evaluation etc.
40
Written
20
Written – Three Hours
40
Basic Biochemistry II Course Schedule: Jan – May 2013
5. COURSE PLAN : - ll –
BASIC BIOCHEMISTRY II (PRACTICAL)
NO
No of
classes
Topics
Techniques
1. Chromatography
10
2. Preparation of normal, molar and percentage solutions.
2
3. Preparation of buffers, pH determination
2
2
Abnormal constituents of urine-sugar, protein, ketones, Blood
2
3
Demonstration
1. Electrophoresis
6
2. Liquid Scintillation system
2
3. Auto analyzer
2
4. Flame photometry
2
TOTAL NO OF CLASSES
28
BASIC BIOCHEMISTRY II (PRACTICAL)
5. COURSE PLAN :
Practical: Basic Biochemistry II
Instructor: Mr. R. Bhuvanasundar/Mr Naresh Kumar
1
Topics
Practical
Batch
No. of
Hours
Chromatography of sugar - single dimension chromatography
2
Chromatography of amino acids - Single dimension chromatography
2
Chromatography of mixtures of sugar / amino Acids
2 dimension Chromatography for amino acids
2
4
EC – I
2
Preparation of normal, molar and percentage solutions
2
Preparation of buffer, pH determination
2
Abnormal constituents of urine-sugar, protein, ketones, Blood
EC – II
Agarose electrophoresis of serum protein (demo)
2
2
4
Agarose electrophoresis of serum lipoprotein (demo)
2
L.S.S. (Radioisotope demo)
2
Auto analyzer, Atomic absorption spectrophotometry (demo)
Comprehensive
2
2
TOTAL NUMBER OF PRACTICAL HOURS
32
FUNCTIONAL AREAS : Exposure to the Biochemistry laboratories - Main and Research at Sankara
Nethralaya.
INSTRUCTOR IN CHARGE
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