Raw-milk pre-calibrated application Immediate measurement of raw-milk quality parameters

Raw-milk pre-calibrated application
Immediate measurement of raw-milk quality parameters
NIRFlex N-500 with Solids Cell, Flow Cell Add-on
Dry-matter, Fat, Protein, Lactose and Criscopic Index measured by means of Near Infrared Spectroscopy
1. Introduction
4. Performances
The fast determination of moisture, fat and protein of
intake raw milk samples optimize the production
operations.
The predictions guaranteed by BUCHI pre-calibrations
are explained by the following tables and plots.
Table 1: Summary of parmeters available in the pre-calibrated
application
Parameter
Range
Dry matter [%]
11.52 – 13.18
Fat [%]
1.99 – 6.85
2. Samples
Protein [%]
2.94 – 3.56
The pre-calibrated application is suitable to analyse cow
raw-milk.
Crisocopic index
The pre-calibration has been created with samples
collected from Italy and other EU customers.
Predicted Property vs. Original Property
Lactose
User Spectra
Predicted Property Dry matter
3. Instrumentation/ Experimental
3.1 Spectrometer configuration NIRSolutions Laboratory:
•
NIRFlex N-500 Solids
•
Flow Cell add-on (measurement)
•
XL add-on (external reference measurement)
•
Laboratory peristaltic pump (6 – 135 mL/minute)
4.22 – 4.98
0.538 – 0.495
User Spectra
Calibration Spectra f(x)=0.9264x+0.9203 r=0.9625 r2=0.9264 Sdev(x-y)=0.0863 BIAS(x-y)= 0 range(x)=11.52 .. 13.18 n=153
Validation Spectra f(x)=0.9002x+1.2440 r=0.9711 r2=0.9431 Sdev(x-y)=0.0788 BIAS(x-y)=-0.003525 range(x)=11.54 .. 12.91 n=56
13.5
13.0
12.5
12.0
11.5
11.0
11.5
B
12.0
12.5
13.0
13.5
Original Property Dry matter
NIRCal : Latte_medie_flusso_solidi totali_OLD 05/05/2010 17.05.35 Administrator
By using a FT-NIR spectrometer it is possible to
determine such parameters in few seconds, just
measuring the sample as it is, with accuracy
comparable to the laboratory reference methods.
All Spectra
User Spectra
Calibration Spectra f(x)=0.9904x+0.0363 r=0.9952 r2=0.9904 Sdev(x-y)=0.0417 BIAS(x-y)= 0 range(x)=1.99 .. 6.85 n=368
Validation Spectra f(x)=0.9839x+0.0568 r=0.9937 r2=0.9874 Sdev(x-y)=0.0383 BIAS(x-y)=0.003688 range(x)=2.95 .. 6.06 n=114
Predicted Property Fat
7
6
5
4
3
2
2
B
3
4
5
Original Property Fat
6
7
NIRCal : Latte_medie_Alfonso_Grassi totali_090310 05/05/2010 16.55.25 Administrator
Predicted Property vs. Original Property
3.2 Measurement principle: transflectance
5. Contacts
For more detailed information please contact your local
BUCHI representative or visit the section “Application
Support” at www.buchi.com.
Inject the sample into the flow cell using a peristaltic pump. Slow
flow rate for a single continuous measurement sequence of 190
scans.
3.3 Sample preparation: stabilize the milk samples at 40°C prior to
measurement. Amount of sample: 50mL (15 mL to prime the cell
prior to the mesurment; 35 mL for the measurement).
Page 1/1