Generic Biosafety Training (2016)

Generic Biosafety Training
(2016)
Objectives
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Raise awareness about scope and authority of the Public Health
Agency of Canada’s Human Pathogens and Toxins Act and Regulation
and the Canadian Biosafety Standards
Direct workers to training resources provided by the Public Health
Agency of Canada
Familiarize biological agent workers and supervisors with the University
of Manitoba’s biosafety program and regulatory framework
Present the U of M’s basic recommended laboratory biosafety
practices, equipment and procedures
Public Health
Agency of Canada
Oversight
The Human Pathogens and Toxins Act (HPTA)
• The HPTA is the law
• It lays out the requirements which must be met in Canada when you
conduct “Controlled Activities”
• Failure to meet the legal requirements is a criminal offence
• See section 53, there can be fines and there can be jail time. This is
not a joke
https://www.burnaby.ca/Assets/RCMP+Musical+Ride.jpg
The Human Pathogens and Toxins Regulation (HPTR)
• The HPTR describes PHAC’s licensing program
which is used to authorize persons to conduct
controlled activities in Canada
• The U of M holds a license under this regulation
• It covers the use of human and terrestrial animal
pathogens and toxins
Inclusions and Exceptions
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Exempt
Pathogens in their natural
environment are excluded from
these regulations.
Foreign animal diseases (not
zoonotic), aquatic animal
pathogens, pathogens of bees,
animal tissues, animal blood
and body fluids or byproducts
are also excluded.
•
Included
Direct manipulation of any
human or terrestrial animal
pathogen in any way is
included (controlled).
Canadian Biosafety Standard 2nd Edition 2015
• This standard is found here:
http://canadianbiosafetystandards.collaboration.gc.ca/cbsncb/assets/pdf/cbsg-nldcb-eng.pdf
• It describes the detailed requirements for working safely with
pathogens and includes everything from facility design to
operational practices and program management.
• These standards must be met to qualify to hold a license to
conduct controlled activities in
Canada.
• These are the performance standards federal inspectors expect
us to meet during compliance assessments.
• Failure to do so jeopardizes our license status.
PHAC e-learning modules
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e-Learning Portal
These modules present
valuable information on
biosafety principles and
practices
They will help you pass the
quiz
This is general lab biosafety
information and not specific to
the U of M.
You will need to register a user
name, password and email
address to log-in to the
modules.
Please review the following modules
• Laboratory Biosafety and Biosecurity
– Principles of Laboratory Safety
1. Microbiology Overview
2. Pathogen Risk Assessment
3. Laboratory Acquired Infections
4. General Safety for Containment Laboratories
5. Containment Level 2 Operational Practices
6. Personal Protective Equipment
7. Biological Safety Cabinets
8. Decontamination in the Laboratory
9. Chemical Disinfectants
10. Autoclaves
11. Introducing Biosecurity
University of
Manitoba
Biosafety and
Biosecurity
Program
Biosafety & Biosecurity at the University of Manitoba
The Biosafety Program at the University was established to
uphold research. The Biosafety Policy and Procedure identifies
and defines the University's commitment to and responsibility for
the safe use of biological materials and agents
Services delivered through the program include:
– Licensure: Applying for and maintaining the University license under the
Human Pathogens and Toxins Act.
– Administrative Oversight Plan
– Providing compliance frameworks and services to lab clients in meeting
the requirements of regulators other than PHAC including:
• Canadian Food Inspection Agency
• Province of Manitoba – Workplace Safety and Health Act and
Regulation
• City of Winnipeg By-laws
• Biosafety regulators from foreign jurisdictions (when collaborating
with partners from foreign countries).
http://www.manfredpichler.com/wpcontent/uploads/2015/01/greekPillar_04.jpg
The administrative oversight plan
includes:
• The University of Manitoba
Biological Safety Policy and
Procedure
• Issuance of Biosafety Permits to
register work with biological agents
• Biosafety Project Approval
Certificates which document the
project based risk assessment and
facilitate the release of grant funds
• Generic Biosafety Training
• Lab inspection templates and
services
• Biosafety Manual
• Providing assistance and signage
for biological lab spaces
Administrative Oversight Plan
University holds institutional HPTA license
University biosafety program meets license requirements
PI’s hold biosafety permits that have conditions
Meeting permit conditions demonstrates compliance with the program
Fulfills the PI’s requirement to be licensed for controlled activities
As long as you are good with us
(biosafety program) you are good with
them (PHAC)
University of Manitoba HPTA License Holder Dr.
Gary Glavin
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Associate Vice-President
(Research)
Holds the institutional
license with PHAC on
behalf of the U of M
U of M Biological Safety
Advisory Committee reports
to Dr. Glavin.
University of Manitoba Biological Safety Advisory
Committee
• Committee Chair 2015-present Dr. R. Dan Gietz
• Advises on the safe use of biological agents at the
University
• Makes recommendations on University Policies
related to biosafety
• Approves standard operating procedures and
guidelines
• Issues and revokes Biosafety Project Approval
Certificates
The Biosafety Team Members
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Biological Safety Officer – Steven
Cole (center left)
[email protected] 204
789-3675
Biosafety Specialist – Vanessa
Pinto (left)
[email protected] 204789-3477
Office Assistant – Darrin Jolicoeur
(center right)
[email protected] 204474-9031
EHS Coordinator – Leona Page
(right) [email protected]
204-789-3613
University of Manitoba Biosafety Manual
The U of M Biosafety Manual
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Fulfils our requirement to have a manual
•
Composed of the main Biosafety Guide
•
There are also a number of appendices designed to
cover other issues not found in the basic guide
The U of M Environmental Health and Safety Assistant
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The main interface for administration of the Biosafety
Program
•
Accessible to both researchers and the Biosafety
Program administrators
Biological Waste Disposal
Chart
Spill Response Guide
Guidelines for the Safe
Handling of Sharps
Bannatyne Post Exposure
Protocol
Fort Garry Post Exposure
Protocol
Biosafety Permits
At the U of M a Biosafety Permit is used to register
a researcher’s facilities, their staff and document
their overarching (general) risk assessment.
• A valid U of M Biosafety Permit is required for all possession
and manipulation of biological agents risk group 1-4.
• To be eligible to hold a Biosafety Permit the applicant must be a
U of M faculty member supervised by a VP, Dean, Director or
Department Head.
• All Biological Workers (anyone who works with live biological
agents) must either hold or be listed on a valid Biosafety Permit
• To start applying for a biosafety permit click here
University of Manitoba: Biosafety Permit System
4-tiered system of containment levels (CL) depending on the risk
group (RG) of biological agents in use. There are no CL4 labs at
the University of Manitoba
CL1/Clinical
• Work with RG1
biological
agents
• Providing
clinical services
• Collecting
clinical samples
from patients or
participants
CL2
Unregulated
• Work with
samples which
are not known
to be
pathogenic
• May be
contaminated
with pathogens
• Blood, body
fluid, tissues etc.
CL2
Regulated
• Controlled
activities
• Human and
Terrestrial
animal
pathogens and
toxins
• Risk group and
containment
level 2
CL3
• Controlled
activities
• Narrow range of
Risk group 3
containment
level 2
pathogens
Determining risk groups and
containment levels
•
Pathogen Safety Data Sheets (PSDSs) and
HPTA schedules can assist with this. These
lists are non-exhaustive; that is, a pathogen
that is not found in the list may require a more
detailed pathogen risk assessment to
determine its risk group.
•
Other resources (microbiology texts etc.) may
be needed to establish the risk level for an
agent if a PSDS is not available.
•
You can contact the BSO or assistance with
risk assessments when you are in doubt.
Risk group 1 – low
individual &
community risk.
Risk group 2 – moderate
individual & low community
risk.
Risk group 3 – high individual
risk & low community risk.
Risk group 4 – high individual
& community risk
REQUIRED: site-specific training for proper use of
pathogens/toxins by supervisor to be documented
Containment Level 1 (CL1)
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CL1 is a basic laboratory with features that
provide the foundation for all containment
laboratories. Biosafety is achieved through a
basic level of operational practices and physical
design features.
Activities with Risk Group 1 pathogens are not
regulated by the PHAC and the CFIA, however
due care should be exercised and safe work
practices should be followed when handling
these materials.
Local Risk Assessments (LRA) are required and
must be reviewed to determine the impact of any
pathogen modifications or when the original
conditions of use have changed.
Best Practices for CL1
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a well-designed and functional space
cleanable work surfaces
use good microbiological practices;
conduct LRAs on activities to identify risks and
to develop safe work practices
provide training
use PPE appropriate to the work being done
employ proper animal work practices.
cleanliness and tidiness - keep laboratory and
animal work areas clean; maintain an effective
rodent and insect control program
decontaminate work surfaces appropriately, in
accordance with biological material in use
PIs working with RG1
biological agents must
hold a valid U of M
biosafety permit
Containment Level 2 Unregulated (CL-2)
• Work with materials which are not expected to
be pathogenic but which may contain pathogenic
contaminants
• This work is not controlled by the HPTA (but
provincial safety regulations still apply)
PIs must hold a valid U
of M Biosafety Permit to
• This work is to be carried out according to the
work with agents
biosafety procedures established in the CBS to classified at this level
prevent accidental exposure to pathogens
• Work is controlled by the HPTA when a
pathogen/toxin is extracted, immunoprecipitated,
concentrated, collected, amplified, cultivated,
refined, cultured, and/or grown
• In this case a CL-2R permit is required
Containment Level 2 Regulated (CL2-R)
• Controlled activities (work) with regulated human or terrestrial animal
pathogens and toxins
• Controlled activities must be conducted according to the biosafety
and biosecurity requirements established in the CBS.
• Permits/labs are under a consolidated license held by the U of M that
is administered by the Public Health Agency of Canada.
PIs working
with RG2
biological
agents must
have a U of M
biosafety
permit to work
with these
agents
Holding a U of
M biosafety
permit equates
to licensure
under the
HPTA
Permit
conditions meet
the
requirements of
the HPTR
Containment Level 3 (CL3)
• CL3 labs are regulated under the
HPTA
• U of M has one narrow scope CL3 lab
facility
• CL3 requirements include
– Stringent facility design and
engineering controls,
– Access is strictly controlled,
– All work is done in a BSC,
– There is HEPA filtration of exhaust air.
PIs working
with RG3
biological
agents must
have a UofM
biosafety
permit to work
with these
agents
Holding a CL-3
biosafety
permit equates
to licensure
under the
HPTA
Clinical Spaces
Clinical spaces (providing clinical services or taking human samples) must be
registered by a clinical space biosafety permit (access the same way as other
biosafety permits)
• Clinical space permit provide waste disposal procedures and hazard
communication systems consistent with other bioagent use areas at the
University
• Workers in clinical spaces are not required to complete generic biosafety
training
• It is expected that these workers have been trained on patient safety and
occupational health and safety as part of their professional training to
provide clinical services to patients and research participants.
NOTE: Collection of human blood samples must follow the
HERC Guidelines for the collection of human blood samples
(HERC- U of M Human Ethics Resource Committee)
Biological Agent Inventory
• HPTA/HPTR requires that an
inventory of all pathogens be
maintained.
• The University
provides the EHS
Assistant Database for
this purpose.
• The inventory is used as part
of the overarching risk
assessment and project
based risk assessment
process.
All live biological agents are to be included in an
inventory which supports a U of M Biosafety Permit:
• Human and animal cell cultures (primary and
immortalized)
• Human and animal tissues
• Human and animal blood and body fluids or samples
• Bacteria
• Viruses (wild type, replication deficient viral vectors or
genetically modified)
• Parasites
• Protozoa
• Fungi and fungal spores
• Mold and mold spores
• Plant viruses, bacteria, fungi
• Algae
• Toxins
• Vaccines
• Prions
• Fixed (non-viable) samples are excluded.
Biosafety Project Approval Certificates
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A Biosafety Project Approval Certificate is used to document a project based risk
assessment.
The certificate must be approved to release grant funds for projects that include
risk group 1-4 biological agents.
An amendment to an approved certificate can be used to add new funds to an
existing project as long as the following conditions are met:
– The agents in use and the procedures in the original certificate are not
changed
– The original grant fund attached to the certificate is still active (still has
funds)
– Biosafety permit information is updated to include any new spaces or
workers included on the project
Import, Export and Transfer of Biological Agents
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You must notify the Biological Safety Officer before you transfer a regulated pathogen in or out of
the University.
If you are unsure whether or not what you are transferring is regulated or not contact the biosafety
officer, they can verify the regulatory status through PHAC resources.
Domestic and international
transfers
Commercial Suppliers:
An electronic copy of the shipping
confirmations/receipts must be
submitted to the BSO
Non Commercial Suppliers:
Biological Agent Transfer Form
filled out and submitted to the
BSO
Transfers within the
University
Bioagent inventory
information must be
updated by the recipient.
Do not supply a transfer
notification to the BSO
For ALL transfers, update of EHSA bio-inventory is required!
Biosecurity – Containment Zones
To facilitate the biosecurity and documentation
requirements of the HPTA containment zones
must be established in all areas where controlled
activities occur.
• Applies for CL2-R and CL3 permitted spaces.
• The guiding principle of biosecurity is the
prevention of the theft loss and intentional
misuse of pathogens, toxins and technologies
that could be used to propagate pathogens
• Refer to the e-Learning Portal and review the
module on Biosecurity for additional
information.
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Biosecurity Plan Requirements
Requirements
Compliance
i. Physical Security
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Keep laboratory doors closed and doors locked when
unoccupied.
Keep all stocks of other organisms locked during off hours.
When research is completed for the day, ensure that chemicals
and biological materials have been stored properly and
securely.
ii. Personnel Suitability and Reliability
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Follow U of M SOPs for hiring graduate students, staff and
faculty
iii. Agent Accountability
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Keep an accurate record of chemicals, stocks, cultures, project
materials, growth media, and those items that support project
activities. EHSA Database
iv. Emergency Response
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Notify appropriate authorities (Security Services and EHS) if
materials are missing.
Ask strangers (someone you do not recognize as a co-worker
or support staff person) to exit the room if they are not
authorized to be there.
Inspect all packages arriving at the work area.
Site-specific response plan
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v. Information Security
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Update passwords for database if authorized personnel leave
the lab
Containment Zone Permit
• At the U of M biosecurity requirements are documented
using a two part containment zone permit system .
Part 1:
Site Pack
Records to be
updated by permit
holder or designate
as required
Provides pertinent
information specific
to the containment
zone and its
operation
Part 2:
Service Pack
To be updated
annually by EHS
References to
standard procedures
developed for use by
all permitted users
by the U of M
biosafety program
Security Sensitive Biological Agents (SSBAs)
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SSBAs are the pathogens which are
considered to be the most dangerous as
biological weapons.
The list of SSBAs is found here: SSBAs
To use these agents you need a special
government issued in depth security
clearance and permit.
Contact us for more information if you intend
to use/exceed trigger quantities for an SSBA
BEFORE ordering or receiving.
These toxins are SSBAs, if you
use these you must contact the
Biosafety Officer BEFORE
ordering:
Alpha toxin
Botulinum neurotoxin
Cholera toxin
Clostridium botulinum C2 and C3 toxins
Clostridium perfringens Epsilon toxin
Hemolysin
Shiga-like toxin (verotoxin)
Shigatoxin
Staphylococcus enterotoxins, Type B
Staphylococcus enterotoxins, types other than
Type B
Staphylococcus aureus Toxic shock syndrome
toxin
Biosafety Training
University of Manitoba
Site-specific training
• You’re doing it right now
• Generic Biosafety Training must
be reviewed annually (by us)
• Online training will be provided
and updated as required
according to the findings of the
review
• Refresher training sessions are
provided for workers with U of M
Generic Biosafety Training prior
to April 2016.
• Includes details about your lab’s
work and procedures not
covered in the Generic
Biosafety Training
• Critical responsibility of the lab
supervisor
• Requires a training plan
• Training must be documented
• Template for site-specific
training
Lab Inspections (Internal and External)
•
All bioagent use labs are subject to inspection by the Biosafety Program Staff.
CL2-Regulated and CL3 spaces will be the subject of Federal Regulatory
Compliance Inspections by PHAC. An inspection program must be in place to
verify that biosecurity and biosafety program imperatives are being met.
Inspections/audits include:
PI lab selfinspection
EHS lab
inspection
PHAC facility
inspection
Access Control
BUILDING
Workplace Hazard Information Placard
(WHIP)
ROOM
P E R S ON AL P R OT E C T I VE E QU I P MEN T (PPE)
RED BORDER = REQUIRED
YELLOW BORDER = COMMONLY USED
R=REQUIRED WHEN WORKING WITH RADIOACTIVE CHEMICALS
All rooms and laboratories where controlled products are
used, stored or handled must have a hazard warning
placard posted at the entrance in order to provide hazard
and emergency contact information.
REFER TO CONTROLLED PRODUCT MSDS FOR SPECIFIC PERSONAL PROTECTIVE EQUIPMENT
2
S PECIFIC H AZ ARDS
The information provided on the WHIP:
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Building and Room Identification
Entrance Requirements
Biohazard symbol with containment level
Required Personal Protective Equipment to enter
Hazards in the Room
Special Precautions
Emergency Contact Information
Hard copy chemical inventory location
First Aid Kit Location
Information for obtaining a WHIP
Containment
Level 2
DATE: 2015
Chemicals
EMERGENCY CONTACTS
Name
TELEPHONE
OFFICE NUMBER
Principle Investigator
Second emergency contact
Third Emergency contact
After normal working hours, call Security Services at “555”, “#555” (using cell phone with Rogers
Wireless or MTS) or dial 474-9341. Hard copy inventories available to EMERGENCY
RESPONDERS located in the Department General Office room___________
MSDS are located in room inside First Aid Kits in room
For Information on lab signage, contact Environmental
Health and Safety Office at 474-6633.
Prepared by:
inside
Responding to Exposures to Biohazardous Agents.
• The University has general post exposure protocols which can
be used in the event of hazardous exposures to pathogens
(links below).
• Print and post the U of M Post Exposure Protocols in your lab.
• Note that there is a separate poster/protocol for each campus
If your work includes the use of uncommon pathogens you may need
to provide a project specific post exposure protocol
• When you work with tropical diseases or pathogens not
commonly found in Canada you use an uncommon pathogen.
Fort Garry Post Exposure Protocol
Bannatyne Post Exposure Protocol
Reporting Exposures to Biological Agents
• If you have been exposed to a biological
agent in the lab it must be reported using
the Notice of Injury Form
• An exposure is considered serious if it
requires follow up medical care, results in
infection or subsequent incidents could be
more hazardous than the first
• Serious incidents must be investigated by
the supervisor, assisted by the BSO and the
investigation must be provided to the
Biological Safety Advisory Committee for
review and follow up
Biological
Agent Incident
Response and
Reporting
Procedure
Notice of
Injury
Form
Reporting Lab Acquired Infections (LAI)
Exposure to a regulated pathogen while
conducting controlled activities which results
in a lab acquired infection or suspected lab
acquired infection must be reported to the
PHAC
•
Reporting a LAI or suspect LAI is required in
the HPTA
•
Failure to report is a criminal offence
•
Required information includes the name of the
institution and the outcome of the investigation
•
Names of workers and supervisors can be
omitted
U of M Incident
Investigation
Form
Lab Biosafety
Lab Biosafety
The guiding principle of biosafety is to prevent worker exposures to
pathogens. Prevention is achieved through:
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Substitution of pathogens for less pathogenic (attenuated) variants where possible
Engineering controls and primary containment devices such as biological safety cabinets,
sealed centrifuge rotors and safety cups or individually ventilated animal cage racks
Administrative controls such as training, safe work procedures and standard operating
procedures which include safety precautions to help workers avoid exposures
Personal protective equipment including disposable gloves, safety eyewear, lab coats,
appropriate clothing and any other safety equipment deemed necessary by risk
assessment
Refer to the e-Learning Portal for additional information.
No Food and Drink in the Lab
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There should be NO food and drink for
human consumption in a lab using biological
agents or hazardous products
Eating and drinking in the lab vastly
increases your risk of ingesting pathogens
and hazardous products
Disposing of food packaging or waste gives
the appearance that food or drink are being
consumed in the lab
http://coastalurgentcarelouisiana.com/food-poisoning-signs-and-symptoms/
http://www.safetysign.com/products/p7044/no-food-or-drink-sign
Biological Safety Cabinets (BSCs)
How they work:
• A downward flow (laminar air flow curtain) of clean High Efficiency Particulate Air (HEPA) filtered
air washes down over the work surface from the air supply above. This flow of air carries any
aerosols you generate toward the waste or dirty air grills
• Waste or dirty air grilles are located at the front and the back of the work surface. These suck up
dirty air contaminated with aerosols from your work and prevent them from reaching the worker
outside the cabinet.
• The dirty air grille at the front of the cabinet also sucks up any contaminants like dust and dirt
before they enter the biosafety cabinet and contaminate your work
• A HEPA filter works by physically trapping particulates with a minimum efficiency of 99.7% at
0.3microns and provides sterile particulate free air
• They DO NOT trap gasses from toxic chemicals or anesthetics. Use a chemical fume hood for
those
• This video demonstrates https://www.youtube.com/watch?v=KqaWM5Dd15c
What makes them malfunction:
•Blocking the dirty air grilles creates holes in the laminar air flow curtain which can allow
aerosols to move in and out of the cabinet.
•Storing large amounts of lab supplies especially in the corners
•Moving too fast or waving your arms into and out of the cabinet
•Damage to the HEPA filter (holes) allows dirty aerosols to escape the cabinet from the
exhaust (exposes workers) and is also recirculated inside the cabinet (contaminates work)
BSCs: Work Layout
uvm.edu
Layout for working "clean to dirty" within a Class II BSC.
• Clean cultures (left) can be inoculated (center); contaminated pipettes can be discarded in the
shallow pan and other contaminated materials can be placed in the autoclave bag (right).
• This arrangement is reversed for left-handed persons.
• Follow the U of M Biohazardous Waste Chart for labeling waste.
• ALL biohazardous material must be packaged/contained OR disinfected before being
removed from the BSC.
Biological Safety Cabinets (BSCs)
At the U of M:
•U of M follows the guidelines discussed in the PHAC module
•All BSCs at the U of M must be certified when first received and then annually thereafter
or at anytime after they are moved. EHS maintains a database of BSCs and annual
certification reports. Certifying BSCs at the U of M.
•At CL2, a certified BSC is to be used for all procedures that may produce infectious aerosols,
involve high concentrations of infectious material or toxins or involve large volumes of infectious
material
•All cabinets must be formaldehyde decontaminated before moving or prior to disposal.
Protection: (Note: Virtually all of the BSCs at the U of M are Class ll BSCs)
• Personal
• Sample Or Product
• Environmental(outside)
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Uses:
•Aerosol producing procedures when working with Risk Group 2 biological agents.
General Rules:
•Don’t disrupt laminar flow by blocking vents, overcrowding, by rapid lateral movements
within or outside the hood, or by using a flame
•Don’t use with toxic volatile chemicals
•Don’t operate with UV light engaged
•All biological waste used in a BSC must be packaged or disinfected before removing.
Biological Safety Cabinets (BSCs): Critical Questions!
It is not OK to discard
contaminated items in containers
(e.g. autoclave bags or sharps
containers) outside of the BSC.
• Frequent movements in an out of the BSC breaks the delicate air curtain and, can leak infectious
material on the cabinets grill and floor (eg. Dripping pipettes).
• ALL biohazardous material must be packaged/contained inside the BSC OR disinfected before being
removed from the BSC. Containers used to collect infectious material inside the BSC must still be
surface disinfected before removing them from the BSC.
Laminar flow hoods (clean
benches) and fume hoods are not
appropriate for work with
biological agents.
•Laminar flow hoods blow the air at your face which is not appropriate for work with
infectious material. Fume hoods have many surfaces and ducts that cannot be easily
disinfected and have no final filtration. Infectious aerosols may be expelled into the
environment. Refer to slide 16 in the BSCs e-learning module.
Letting the BSC run for five (5)
minutes does not replace surface
disinfecting all items before
removing them from the cabinet.
•ALL biohazardous material must be packaged/contained OR disinfected before
being removed from the BSC. Refer to slide 16 in the BSCs e-learning module.
You cannot count on UV lights for
surface disinfection in place of
appropriate disinfection.
•UV lights have limitations. Refer to slide 22 in the BSCs e-learning module for a full
discussion.
Personal Protective Equipment (PPE)
Protect your clothes and skin from contamination
by pathogens and hazardous products. Reusable
PPE are not to be laundered at home.
Lab coats are worn when
conducting controlled
activities and while working
with hazardous products
Should wear them at all times
when in the lab.
Do Not
wear lab
coats/
gloves in
the
following
areas:
• Public spaces
• All offices, bathrooms,
elevators, public hallways
• Coffee/ lunch rooms,
departmental libraries
• Restaurants
• Student study desks/area
outside of the lab
• Cars, trucks, busses and vans
Thinkgeek.com
Lab Coats
Gloves: Types and Characteristics
Latex:
a natural rubber
•resists water, acids, alkalis, salts, ketones
Nitrile:
a synthetic rubber
•better chemical protection, superior
puncture and abrasion protection, better
electrostatic dissipation
Neoprene:
a synthetic rubber
•superior chemical protection, good for
handling acids, caustics, alcohols, solvents
Vinyl:
polyvinyl chloride
•short-term protection against acids, caustics
and alcohols
Combination
gloves:
•latex & nitril
•neoprene & latex
•latex & nitrile & neoprene
Heat & Cold
resistant gloves
Do Not wear gloves in the lab
when touching:
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Door knobs
Computer keyboards
Telephones or cell phones
Personal electronic devices
Always wear when working
with infectious agents!
• Always wash your hands
after removing your Gloves!
Eye Protection
Eye
protection/
goggles
must be
worn
when:
• retrieving samples from
liquid nitrogen
• working with UV light
(UVA&B filtering face
shield)
• handling caustic or
dangerously reactive
chemicals
• cleaning chemical spills
(non-vented goggles)
Basic safety
glasses
Impact-Only
Resistant Safety
Goggles
Chemical and
Vapour Resistant
Safety Goggles
• Direct vent holes
therefore not chemical
splash and vapour
resistant
• no direct
vent openings
Face Shield
Standard face
shield
• Should be used when there is a
risk of flying objects which may
strike the face and eyes
Advantages
• May better accommodate
corrective eyewear
• Less fogging
• Wide range of applications and
adaptations.
Disadvantages
• Gaps at the side of standard
shields may offer incomplete
protection especially from
splashes by hazardous liquids
Standard
clear
Specialty
ventilated
Use of a face shield should be
documented as part of the risk
assessment.
Special
shield
applications
Footwear
•
•
•
Protective Footwear must be selected based on risk
asessment of the worksite and potential Hazards.
Footwear should protect the entire foot from
hazardous liquids and be easy to clean and/or
disinfect.
Completely enclosed footwear with no heels or low
heels are recommended to be worn in the
containment zone to reduce the risk of exposure to
infectious material or toxins in the event of an
incident or accident.
NO!
At the U of M, in ALL
LABS, completely
enclosed footwear with
no heels or low heels
are required unless a
documented LRA can
show otherwise.
Shoes should have a
closed toe and heel
and cover the entire top
of the foot. Ballet flats
for women, and mesh
runners, technically
cover the toes and heel
but do not enclose the
top of the foot.
Decontamination in the Laboratory
Waste Disposal
•
Segregate Biological from Chemical from Radioactive waste at
source.
•
NO sink disposal of chemicals.
(with very limited exceptions as per U of M waste disposal chart)
•
Develop waste disposal procedures before starting work.
•
Generation of mixed waste (e.g. radioactive and biological) may
need special procedures and approval.
•
Questions should be directed to the Biosafety Officer or
Occupational Hygiene Coordinator
Biohazardous Waste: Solid and Dry
•
•
•
BEFORE you start working, know how you will
dispose of your items and have appropriate,
clearly labeled containers available.
If you don’t autoclave your waste at the end of
every day, your biohazardous waste container
/support stand must have a lid. A container or
support stand with a step-on-mechanism (handsfree) is required.
Aspirate or pour off all liquids before disposing of
the item in an autoclave bag. For example, if you
have extra cell suspensions, tissue culture media
or supernatants, treat these with the appropriate
concentration of chemical disinfectants or
autoclave instead.
Cardboard boxes are not an appropriate support
stand for use as a biohazardous waste container!
(They can’t be disinfected)
•
Container which can be
easily disinfected
•
Plain Clear Autoclave Bag
•
Large Biohazard Sign
Biohazardous Waste: Solid and Dry
What can go into an autoclave bag?
YES
 Petri dishes
 tubes & other
NO
X Anything contaminated with
chemicals or radio-isotopes
 plasticware
 gloves
 culture flasks
 bench paper
X Anything that can puncture
the bag or skin
Biohazardous Waste: Autoclaving
Fill the bag only to
the level where it can
be loosely closed,
typically 2/3 full.
• During autoclaving the bag must
be open enough to allow steam
to access contents!
Label it and
submit for
autoclaving.
• NOTE: Autoclave tape will only indicate if the
item has been processed, not that the
contents are sterilized.
Place the autoclave bag in
secondary containment for
transport and autoclaving
to prevent leaks and spills.
• The secondary container
must be made of a
material which can
survive autoclaving
without failing
After autoclaving place in a dark,
opaque, garbage bag, tie it shut and
dispose as regular garbage with the
caretakers.
Biohazardous Waste: Sharps
Sharps that must be
disposed in an approved
sharps container include
the following:
• Needles & syringes,
scalpel/razor blades
• Biomedical Waste Sharps• items which could
potentially puncture the
skin and are contaminated
with human/ animal tissue,
fluids or blood
• eg pipette tips
For other potential
sharps contaminated
with microbial
substances: e.g.
bacteria, viruses and
tissue cultures
• Collect these in rigid,
puncture-resistant,
autoclavable containers.
• Containers can be reusable.
• Label the container with the
Biohazard sign.
• Autoclave and dispose as
regular glassware waste.
Approved sharps
containers are:
• rigid, puncture-resistant,
leakproof
• has a securable lid
• appropriately labeled
• autoclaved if biologically
contaminated
• discarded through the EHS
DON’Ts
• remove needles from
syringe or recap
• bend needles
• overfill containers – ie,
NOT MORE THAN ¾ FULL
• autoclave chemically or
radioactively
contaminated
needles/syringe,
razor/scalpel blades.
These are placed in
separate containers,
labeled appropriately and
disposed through EHS.
Biohazardous Waste: Critical Questions!
Disposal of potentially/contaminated sharps
according to the U of M Biohazardous Waste
Chart.
CBS Section 4.6.9.
CBS Section 4.6.10:
• Sharps items which could potentially puncture the skin and are contaminated or
potentially contaminated with human/animal tissue, fluids or blood or other
infectious material can pose a biological hazard and a physical hazard before and
after autoclaving.
• Use of needles, syringes and other sharp object to be strictly limited.
• Bending, shearing, recapping, or removing needles from syringes to be avoided,
and, when necessary, performed in accordance with SOPs.
Safety-engineered sharps should be used
whenever possible.
An “approved” sharps container for these
items
• rigid, puncture-resistant, has a secure lid, and appropriately labeled,
• OR autoclaved if biologically contaminated and discarded through the EHS.
Pasteur pipettes, serological pipettes and
pipetteman tips (all sizes) cannot be collected
in autoclave bags for autoclaving.
• These items can all poke through an autoclave bag, potentially injuring lab,
autoclave or caretaking personnel.
Biohazardous Waste: Liquids and Pathological
Liquid Biohazard
Waste
(non-chemical, nonradioactive)
If treated with bleach or
autoclaved at 121°C as
appropriate for volume
Pathological Waste
(e.g. animal body parts
and carcasses)
Double bag and store
carcasses and all
related material in the
freezers designated by
Central Animal Care
Services (CACS)
Mixed Radioisotope
and Biological Waste
(Rad Biowaste)
Requires pre-approval
from Radiation Safety
Program and the
Biological Safety
Officer
Dispose in sink with
copious amounts of water
DO NOT DRAIN DISPOSE
AGAR MEDIA!
Cautionary Reminder: How to use Bleach
You must base your dilution calculations to make solutions
with a given % Sodium Hypochlorite
Bleach is the common
name for a 5.25%
solution of sodium
hypochorite (NaClO).
The final effective
concentration for
decontamination
should be ~0.5-1%
sodium hypochlorite.
0.5% for disinfection
1% for spill clean-up.
•READ the LABEL as
household bleach comes
in different
concentrations!
Instructions may indicate
to make a 10% solution
or make a 1/5 or 1/10
dilution.
(Initial conc. = 5.25%)
•1:10 -(final conc. 0.5% NaClO)
- when mixed with media to
disinfect supernatants or liquid
cultures
•1:5 -(1.05% NaClO) -effective
for biohazardous spill clean up
Cautionary Reminder
Bleach (sodium hypochlorite) will corrode
stainless steel if left in contact for an
extended time.
• To disinfect stainless steel equipment (BSC, incubators,
water baths, sinks) use 70% ethanol or another noncorrosive disinfectant.
• If you must use bleach to clean stainless steel equipment
be sure to rinse off all the bleach with water.
DO NOT mix bleach with an acid or any
other cleaners.
• If you mix bleach with other chemicals it may cause a
release of poisonous chlorine gas
• Only mix bleach with clean water prior to use.
• Do not mix with chemical wastes
Critical Question:
Make fresh dilutions weekly.
Dilutions can deteriorate
quickly.
Even store bought concentrations
can deteriorate over time. Check
expiry dates on the bottle.
Avoid pipe corrosion!
When pouring bleached solutions
down the sink, be sure to fill the
sink ¾ water THEN add your
bleached solutions! DO NOT pour
bleached solutions directly down
the sink!
How to Use Ethanol
The optimal
ethanol/isopropanol
solution for
disinfection is 70%
Use a wash bottle
(see below) instead
of a mist type spray
bottle. If alcohol is
sprayed onto
surfaces in a mist it
evaporates before
making contact and
degrades the
activity
CAUTION! This solution of alcohol is flammable, do
not expose to open flames, sparks, oxidizers or
sources of ignition especially when wiping onto
open surfaces!
http://www.capitolscientific.com/Azlon-506455-0002-250mL-Driplok-Right-to-Know-Wash-Bottle-for-Ethanol-w-Label-Safety-VentedL
Flood the surface to be
cleaned with 70%
ethanol/isopropanol
and wipe evenly onto
the surface with a
clean paper towel.
Leave to dry.
Safety Showers and Eye Wash Stations
3
2
1
1. Hands-free sink
2. Eyewash station
• if it is located inside
the lab, it must be
flushed weekly by
lab personnel
3. Safety shower
4. Fire Extinguishers
5. Smoke alarms
6. Sprinklers
Locate and memorize the location
of your eyewash stations, safety
showers and fire extinguishers!
4
Biohazardous Response Plans
The specific steps that you follow will
depend on:
•Restrict access to area for 30 minutes to allow aerosols to
settle.
•While wearing protective clothing, gently cover the spill with
paper towels or absorbent pads.
•Flood the absorbent with an appropriate disinfectant, starting at
the perimeter, working inwards towards the centre.
•Use a gentle flooding action to reduce the creation of aerosols.
•Allow sufficient contact time before clean up (refer to product
information sheets for the disinfectant in use).
•Use forceps to pick-up any broken glass or sharps and place
these in a leak-proof puncture resistant container.
•Also note•Spills should never be cleaned up by untrained staff.
•Caretakers are not allowed to clean up any lab spills.
•The best time to learn about and practice cleaning up a spill is
before it happens.
6 key techniques to
prevent the release of
contaminated material
during a spill clean-up
PHAC Job-Aid on Spill
Clean-up Procedure
•the nature and concentration of your pathogen,
•whether the spill is small or large,
•inside or outside of the biosafety cabinet or
•associated with some other lab equipment.
Every lab that contains controlled products
should have a basic spill kit.
•Spill kit location and use must be known by all lab
members
•Print spill clean-up procedures and put them in a
plastic sleeve inside your spill kit.
•U of M Biohazardous Spill Kit and Response
Guidelines
Learn and practice the site-specific
spill response protocol for your lab!
If none are available, meet with your
co-workers to develop and practice
your plans.
Basic Lab Spill Kit
A “Basic Lab Spill Kit” could include the following:
1) A five gallon pail with lid for kit storage or to act as a waste pail in the event of a
spill.
2) Universal absorbent pad and granular absorbent.
3) Disposable and reusable chemically resistant gloves –for two people.
4) Two pairs of non-vented splash goggles.
5) Caution tape and duct tape.
1
6) Sealed plastic transfer pipettes.
3
7) Dustpan and brush
- autoclavable or disposable.
2
7
8) Garbage bags and autoclave bags.
5
9) Face shield
4
3
10) Mercury Spill kit if items containing
8
mercury are available in the lab.
General Lab &
Personal Safety
General Lab Spill Cleanup
Personal Safety
Working Alone Policy
Fire Safety
&
HOW TO SUBMIT YOUR
QUIZ
Fume Hoods, Laminar Flow Hoods
Fume Hoods
How they work:
• Draw air from the lab through an opening in the sash. Contaminated air is expelled out an exhaust duct on the
roof of the building. There is generally NO filtration of contaminated air. U of M air flow standards require a
face velocity of 80-120 lfpm at a sash height of 11in (30cm).
• Look for the safe operating stickers found to the side of the glass sash.
Protection:
• Personal
• Sample or product
• Environmental (outside)

X
X
Uses:
• Laboratory work involving poisonous, corrosive, odorous or flammable chemicals (if appropriate). Not for use
with biological agents.
General Rules:
• Work with the sash height indicated on the sticker
• Work at least 15cm (6”) behind the face of the sash
• Do not block the rear baffles
• Do not use the fume hood to dispose of solvents by evaporation
• Do not use for general chemical storage
Fume hoods should NOT be a chemical or equipment storage area, or permanent radioactive
workstations. If your hood lacks space to comfortably decant a 4L bottle of solvent into a
beaker then it REQUIRES CLEANING (discuss with your Principal Investigator / Supervisor)
Laminar Flow Hoods
How do they work:
•Also called clean benches, these are specially designed cabinets that provide
HEPA filtered laminar flowing air over the work space and into the room. Can be
vertical or horizontal laminar flow hoods.
•Do not confuse horizontal models with BSCs!:
Protection:
•Personal
•Sample Or Product
•Environmental(outside)
X

X
Uses:
•For lab work requiring a sterile environment. For example media preparation or
plant culturing is fine but NOT human or animal tissue or cell culturing.
General Rules
•Don’t disrupt laminar flow by blocking vents, overcrowding, by rapid lateral
movements within or outside the hood.
•Don’t use with chemicals or >RG2 infectious material.
•Don’t disinfect with bleach alone unless followed with appropriate rinse. Bleach
corrodes stainless steel.
Personal Safety
Code Blue Station
• Provide 24/7 instant voice connection to Security Services
• Officer will automatically be sent and arrive in under 2 minutes
• Strategically placed at both campuses
Safewalk Program Call 474-9341
• For students, faculty, and staff
• Available at BOTH campuses,
• Provides a safe walk to your car or other building at night. (A
student patrol with specially marked security vests or a uniformed
Security Services Officer )
Red Emergency Buttons:
• available in certain buildings and tunnels. Pushing the red button
will alert Security Services that an emergency exists at that
location.
New Red Call Boxes:
• two-way communication boxes with Security Services.
Departmental Emergency Contacts and Safety Information
Labs / Departments must have safety and emergency information
provided to ALL employees. See example below.
Lab Location: Rm 807-808 BMSB
PI: Dr. Robert Safety
789-2121
Eyewash/Safety Shower:
Hallway by Room 456
Fire Extinguisher
Hallway by Room 123
Fire / Medical Emergency 555
UM EHS (Environmental Health &
Safety Office)
CHEMICAL / RADIATION / BIOHAZARD
8:30am-4:30pm
474-6633
Outside Regular Hours
474-9341
(MICB -also call HSC security 74567)
Dept. WHMIS Coordinator:
Mr. Martin Keener
789-2122
Dept. Radiation Coordinator:
Dr. Isaac Aymhot
789-3333
MSDS Location:
North bookcase Rm 807b
UM EHS Website:
http://umanitoba.ca/admin/vp_admin/risk_manage
ment/EHS/
Fire Wardens:
Mr. Donny Flame
Dr. Johnny Nitro
789-2133
789-2124
SAFETY INFO & FIRST AID KIT:
Hallway outside Rm 808
SPILL KIT Locations:
Under sink Rm 807
Spills: General Guidelines
Glassware breakage
• Sweep up broken non- contaminated glass and discard as glassware waste
Radioactive Spills
• NOTE: Only personnel listed on a U of M Internal Radioisotope Permit are allowed
to clean-up Radioactive Spills!
• Minimize contaminated area,
• Minimize amount of waste.
• Do not accidentally spread contamination.
• Wear a lab coat and disposable latex or nitrile gloves.
• Absorb spill with minimal absorbent material.
• Clean area with damp towel from outside in, monitoring success of cleanup.
• Discard all soiled materials as radioactive waste (yellow tags)
• All spills MUST be reported to EHS’s Radiation Safety Officer (Cell: 204-298-3769 or
E-mail [email protected])
Non-hazardous liquids and solutions
• Wear a lab coat and spill kit gloves and dust mask (if necessary).
• Sweep up solid chemical and broken glass and discard in a waste container labeled with the
UM waste tag. Once done, cap and arrange for disposal via U of M EHS.
• For liquids, absorb with spill kit absorbent or paper towels or with a mop and discard in a
waste container labeled with the UM waste tag. Arrange with UM EHS for disposal. Wash
the area with warm soapy water.
• Clean and replace all cleanup equipment.
Hazardous liquids and solutions
• U of M Chemical Spill Procedures
• A spill is defined as an uncontrolled release of a chemical. Spills can be categorized into two
types.
• Major spills require an external emergency response, ie. Winnipeg Fire Department.
• Minor spills are spills that do not meet the criteria of a major spill and can normally be dealt
with by University personnel. EHS can provide technical advice or onsite assistance.
• Caretaking/housekeeping staff do not have the training to clean up a chemical spill.
• You will not attempt to clean up the spill if the cleanup is beyond your capabilities.
Departmental Working Alone Policy
Review your Department’s “Working Alone Policy”
Workplace Safety and Health Act recognizes that certain workplaces require staff to
work alone and therefore requires that a plan is in place that is agreeable to both
the employer and employee. The plan should be based on a realistic risk
assessment of the hazards under the circumstances and include a written
emergency response plan.
Policies might include:
•
•
•
•
•
Mandatory buddy system where staff and students must work in pairs.
Supervisor notification before starting work after hours.
Restrictions to certain types of work.
Restrictions to certain employees or employees with specific training.
Mandatory use of “Safewalk” service to get to your car.
U of M Governance Procedures - Working Alone
Emergency Response Plans
U of M EMERGENCY PHONE NUMBERS
U of M EMERGENCY QUICK REFERENCE
eg. medical emergency, fire, assault, violence, traffic accident
At U of M
At HSC / RIOH / KIAM
• Dial 555 OR 911 from any U of M telephone exchange (e.g. 474-, 789-,
975-) Dial (204)474-9341 from all other phones
• Dial #555 from any Rogers or MTS cell phone
• Dial 55 (not 911) from any 787 - telephone exchange
All 555or 55 calls go directly to Security Services who will send the appropriate
response and also accompany outside emergency vehicles to the correct
location
Fire Safety
EVERYONE is expected to leave the building when the alarm sounds!
•
Get to know the alarm bell scenarios in any of the buildings where you work.
•
When you hear a fire alarm you should turn off all flames and gas sources and
prepare to exit the building via the stairway. Know what to do if you are in the
middle of an experiment.
•
Discuss and plan emergency response with your supervisor and
departmental fire warden ahead of time. If required, call EHS (474-6633) for
assistance with your plans.
•
During a fire alarm, the fire warden for the floor will usually be wearing a RED
neck pouch that says FIRE WARDEN.
Submitting your Biosafety Quiz
Thank you for completing the University of
Manitoba Generic Biosafety Course.
o The quiz is based mostly on the PHAC e-learning
modules and this presentation. Some answers
will be found in the powerpoint presentation.
o To pass you must obtain a mark of at least 80%
and also answer the seven critical questions
correctly.
http://www.laclosette.com/wp-content/uploads/keep-calm-and-ask-us-15.png
o You must now complete and submit the Biosafety
Quiz
Environmental Health and Safety Location and Contact
Fort Garry Office
191 Extended Ed.
Phone 204-474-6633
•
•
•
•
•
•
Bannatyne Office
T248 Old Basic
Science Building
Phone 204-474-9031
• Biosafety
• Radiation Safety
Risk Management
Occupational Health
Industrial Hygiene
Fire and Life Safety
Chemical Safety
Hazardous Waste Disposal