BGEN 7020 PROTEINS Winter 2016

BGEN 7020
PROTEINS
Winter 2016
Course Outline
Course Outline
Course Title:
Proteins
Course Number: BGEN 7020
Term(s) Offered: Winter 2016
Course Description:
The course will deal with the expression, purification, bioinformatics,
characterization, structure, folding and engineering of proteins. Each student will be
required to carry out a lab research component and write a 3-5 page report and
present a seminar.
Academic Unit (Department) Responsible:
Biochemistry and Medical Genetics
Course Coordinator:
Name:
Dr. R. Bhullar
Office:
Room D325
Phone:
789-3703
E-mail:
[email protected]
Office Hours:
Contact by e-mail
Instructor 1: Dr. P. Chelikani
Phone: 789-3539
E-mail: [email protected]
Lecturer 2: Dr. J. Wilkins
Phone: 789-3835
E-Mail: [email protected]
Instructor 3: Dr. B. Mark
Phone: 480-1430
E-mail: [email protected]
Teaching Assistant: Mr. Appalaraju (Raju) Jaggupilli
E-mail: [email protected]
Grading Scale:
A+=90-100%
B+=75-79.9%
A=80-89.9%
B=70-74.9%
C+=65-69.9%
F=0-59.9%%
C=60-64.9%
Passing Mark:
The passing mark for this course is a final overall mark of 60%.
Attendance:
Students are expected to write examinations on the scheduled dates. Students with
unexcused examination absences will be given a mark of zero (‘0’) for the exam. To
obtain a deferral for an examination, students must:
a) give notice of the absence prior to the examination; and,
b) submit acceptable documentation (i.e., physician’s certificate) of the illness.
Evaluation
Examination Type
Date
% of Final
Mark
Presentation/discussion
Jan 19
20%
Presentation/discussion
Jan 26
20%
Mar 8
40%
(TBA)
20%
Lab report &
Seminar presentation
Written exam or take home exam
DETAILED COURSE SCHEDULE:
Date
Time
Topic
Basics of protein structure,
expression and purification:
Amino acids, peptide bond,
Jan. 12
2 – 5 pm
primary to quaternary structure,
protein expression
(soluble/membrane bound),
purification (affinity tags)
Jan. 19
2.00-3.30pm
3.30-5.00 pm
Jan. 26
2.00-3.30pm
Instructor
Dr. Bhullar
Location:
Biochemistry
Seminar Room
Presentation & discussion (15
min/student)
Drs. Bhullar /
Chelikani
Protein structure folds, domains,
motifs, evolution, introduction to
ExPASy
Dr. Chelikani
Presentation & discussion (15
min/student)
Drs. Bhullar /
Chelikani
Bioinformatics, Protein structure
to function, soluble proteins,
3.30-5.00 pm membrane proteins, sitedirected mutagenesis, SNPs
(genetic variants)
Each student shall build two
protein models: A key enzyme
For timing
Feb. 2 Adenylyl cyclase (4 hours), and a
check with
Feb. 16
membrane protein, Taste
TA
receptor-T2R4 (4 hours) using
Discovery Studio (DS) suite
Lab project: To build a molecular
model of a protein given by the
For timing instructors. Involves student
Feb16 check with spending 4-6 hrs in lab building a
Mar. 1
model and then writing a 3-5
TA
page report (Brief introduction,
Detailed methodology, Results &
Dr. Chelikani
Mr. Jaggupilli (Raju)
Discussion)
Mar. 8
2 – 5 pm
Mar. 15
2 – 5 pm
Mar. 22
2 – 5 pm
Mar. 29
2 – 5 pm
Presentation of seminar (15
min/student) on lab report
Protein-protein interactions:
methods of characterization;
applications/examples; concept
of protein ‘networks’
Proteomics: methodology;
equipment; analysis; current and
future applications
X-ray crystallography:
introduction; techniques;
equipment; structure
determination and analysis;
current applications
Written exam (or take home
exam; Format to be determined)
Drs. Chelikani /
Bhullar
Dr. Wilkins
Dr. Mark
Dr. Bhullar
ACADEMIC INTEGRITY:
There is no tolerance for academic dishonesty and students should be aware that consequences for acts of this nature
could include expulsion from the graduate program. Further information on penalties may be found under the Student
Discipline By‐Law via the following link to the U of M homepage
(http://www.umanitoba.ca/admin/governance/governing_documents/students/868.htm ).
Academic dishonesty is the act of presenting other peoples work as your own and includes among others, inappropriate
submission of original work, examination personation, failure to acknowledge contributions of others as well as
inappropriate collaborations (group work). Academic dishonesty includes presenting unmodified sentences from an
external source such as a published manuscript without proper citations.
ATTENDANCE:
Students are required to attend all scheduled classes and exams. Students arriving >10-minutes late will be recorded as
absent. Absence from an examination will result in a mark of “Zero” unless the student provides prior notification and
subsequent documentation (physician’s certificate required for illness) of the absence.
Deferred/Make-up Exams/Late assignment:
Examinations that, due to student absence, must be re-administered at times other than the scheduled period are
highly disruptive to the course sequence. They require the scheduling of a testing location and the assignment of
faculty to proctor the exam. The different date has the potential to afford an unfair advantage to a student. The course
director may, at his/her discretion, choose to alter the format of a re-administered examination to accommodate the
above concerns. A re-administered examination may include one or a combination of short answer, essay, or multiple
choice questions, and can be in either oral or written format.