Name: Boris S. Ermolinsky Date and place of birth:

Curriculum vitae
I.
BIOGRAPHICAL AND CONTACT INFORMATION
Name:
Date and place of birth:
Citizenship:
II.
EMPLOYMENT
2007-present
2006-2007
2002-2006
1993-present
1985-1993
III.
2002-2006
1995-2000
1979-1985
IV.
Boris S. Ermolinsky
September 21st, 1960; Ust-Kamenogorsk, Kazakhstan
Russian, Green Card
Research Assistant Professor, University of Texas Brownsville (UTB)
Lecturer, Dept. Biological Science, University of Texas Brownsville (UTB)
Postdoctoral Fellow, School of Public Health, University of Texas Health
Science Center Houston – Brownsville regional campus
Senior Research Scientist, Engelgardt Institute of Molecular Biology of Russian
Academy of Sciences, Laboratory of Stereochemistry of Enzymatic Reactions,
Moscow.
Research Assistant. USSR Research Institute of Phytopathology, Laboratory of
Molecular Biology, Moscow region.
EDUCATION
Postdoctoral training, School of Public Health, University of Texas Health
Science Center Houston – Brownsville regional campus
Ph.D. in Chemistry, Engelgardt Institute of Molecular Biology Russian
Academy of Science, Moscow, Russia.
M.S. in Chemistry, Moscow Institute of Fine Chemical Technology.
SKILLS
Computer literacy: general computer skills in DOS, Windows, MS Word, MS Excel, Paint
Shop Pro, ChemWindow, ISIS/Draw, ISIS/Base, HyperChem.
The basic skills of operation in the Internet)
Languages: Russian, English
Laboratory Techniques:
Organic chemistry: General organic synthesis, synthesis of nucleotide-nucleoside
derivatives, synthesis of modified oligonuleotides, purification and analysis of
synthesized compounds.
Biochemistry: Enzymatic synthesis of biochemical compounds, methods of enzymatic
kinetics, methods of modification of antibodies, protein analysis, protein
purification.
Molecular biology: molecular cloning, immunochemistry, design and preparation different
vectors for molecular cell biology.
I
General skills of tissue culture, immunohystochemistry, design and setup
methods of transfection of different lines of cells, design and setup methods of
analysis of expression different genes by RT QPCR.
Instrumentation: electrophoresis, HPLC-technique, LC-MS of proteins, ELISA, QPCR,
imaging, microscopy, centrifugation, UV, IR, mass spectrometry, NMR
spectrometry.
V.
MEMBERSHIPS
2011-Present
2007-Present
2006-Present
2006-Present
VI.
2010
American Chemical Society
Center for Biomedical Studies (CBS) at UTB/TSC
American Society of Mass Spectrometry
American Association for the Advancement of Sciences
AWARDS AND HONORS
2006
2005
Certificate of Appreciation, University of Texas at Brownsville, the Biological Chemical
Radiation Safety Committee.
Certificate of Recognition, Texas A&M University System and Cameron County.
Certificate of Appreciation, School of Public Health, University of Texas Health Science
Center Houston – Brownsville regional campus.
VII.
2009
2006
2004
2004
2004
2004
CERTIFICATIONS
Shipping Infectious Substances and Diagnostic Specimens, UTB
Shipping Infectious Substances and Diagnostic Specimens, UTB
Radiation Safety Training Course, UTB
Shipping Infectious Substances and Diagnostic Specimens, UTB
Blood borne Pathogens Training Course, UTB
Laboratory Safety, UTB
VIII.
COMMITTEE APPOINTMENTS
2005-present Radiation Safety Officer and member of the Biological-Chemical-RadiationSafety Committee, UTB
XI.
FUNDING
2009-present Research Infrastructure in Minority Institutions (RIMI). Subproject#1: Aptamerdirected nanocomplexes for targeted transfection. Role : PI.
2007-2009
Grant from the National Institute of Health, National Institute of Neurological
Disorders, 1R21NS056160-01A2 (Garrido-Sanabria, PI). Targeted
immunoliposomes for cell-type specific gene therapy of epilepsy. Role: Co-PI
2004-2006
National Center on Minority Health and Health Disparities (NCMHD). Creation
of an Hispanic Health Research Center in the Lower Rio Grande Valley
(EXPORT). Role: Co-Investigator
II
2004-2005
NIH-EXPORT PILOT grant, UTHSCS/SPH, Development of new screening
methods for cervical cancer in low income women. Role: PI
Russian foundation for basic research. Affinity modification and inhibition of
enzymes by oligonucleotides with reactive dialdehyde groups. Role: PI
2001-2003
X.
INVITED SEMINAR PRESENTATIONS
2005
XI.
Border Health Seminar Series, University of Texas Brownsville - School of
Public Health, University of Texas Health Science Center Houston – Brownsville
regional campus
TEACHING
i.
2010-2011
2008-2009
2006-2008
2003-2006
2003-2006
2003-2006
Tarun Pandari (Master`s Thesis), UTB
Frank Skinner (undergraduate student at UTB, Major in Biology)
Masoud Arshadmansad (undergraduate student at UTB, Major in Biology)
Robert Wilson, (undergraduate RISE student), UTB
Angel Olguin, UTB (Master’s Thesis), UTB
Alisande Cardenas (Master’s Thesis), UTB
ii.
2008-2009
2006-2008
2006-2009
2006-2007
2006-2007
2006-Present
2006-2007
2000-2002
XII.
Students mentored in the laboratory:
Lectures and laboratory courses:
General Biology I laboratory, undergraduate students, UTB
Organic Chemistry I and II Lab, undergraduate students, UTB.
General Chemistry I laboratory, undergraduate students, UTB.
Cell and Molecular Biology laboratory, undergraduate students, UTB
Advanced Physiology Laboratory, undergraduate students, UTB
General Biology I laboratory, undergraduate students, UTB
Immunology, laboratory and lectures, undergraduate and graduate students, UTB
Chemistry and Structure of nucleic acids, Doctoral students
PEER REVIEWED JOURNAL ARTICLES
1.
Beigelman L.N., Gurskaya G.V., Tsapkina E.N., Karpeisky M.Ya., Ermolinsky B.S.,
Mikhailov S.N. A new synthesis of 2'-C-methylnucleosides starting from D-ribose. Nucleic
Acids
Symp Ser V.18, p. 41-44, (1987).
2.
Beigelman L.N., Gurskaya G.V., Tsapkina E.N., Karpeisky M.Ya, Ermolinsky B.S.,
Mikhailov S.N. New synthesis of 2'-C-methylnucleosides starting from D-ribose and Dglucose Carbohydrate research, V.166, 219-223, (1987)
III
3.
Khurs E.N., Voinova T.M., Ermolinsky B.S., Dzavakhia V.G., Khomutov R.M. New
inhibitor of AcCoA dependent biosynthesis of the natural products. Abstracts of 14-th
International Congress of Biochemistry, Prague, Czechoslovakia, July 10-15, 1988, V.II,
Tu.665, p.233
4.
Khurs E.N., Voinova T.M., Ermolinsky B.S., Dzavakhia V.G., Khomutov R.M.
Antipenetrant action of 1-aminoethyl phosphorus acid which inhibits conidia genesis of
phytotoxine of fungus Pyricularia oryzae Cav. Book of abstracts 7-th International Congress
of Pesticide Chemistry. Hamburg, August 5-10, 1990, 04D-08, 236
5.
Khomutov R. M., Khurs E. N., Biryukov A. I., Zhukov Yu. N., Dzhavahia V. G., Voinova
T.M., Ermolinsky B. S. Alaphosphalin (L-alanyl-L-1-aminoethylphosphonic acid) inhibits
Ac-CoA-dependent pathways of micromycetes biosynthesis. Russian Journal of Bioorganic
Chemistry 1990, 16 (2):275-279
6.
S. I. Sviridov and B. S. Ermolinskii. Secondary metabolites of Pyricularia oryzae I. ONitrophenol derivatives. Chemistry of Natural Compounds, V.26, N6 / Nov., 1991, 691-696
7.
Mikhailov S.N., Fomitcheva M.V., Efimtseva E.V., Ermolinsky B.S., Meshkov S.V.,
Esipov D.S., Boldyreva E.F., Korobko V.G. Oligodeoxyribonucleotides containing 1--Dglucopyranosylthymine : synthesis and substrate properties. Coll. Czech. Chem. Comm.
Special Issue, 61, 222-225 (1996).
8.
Mikhailov S.N., Efimtseva E.V., Ermolinsky B.S., Fomitcheva M.V., Gritsenko O.M.,
Brevnov M.G., Gromova E.S., Schepers G., Van Aerschot A., Herdewijn P. Regioselective
incorporation of reactive dialdehyde groups into synthetic oligonucleotides. Coll. Czech.
Chem. Comm. Special Issue, V.61, 210-212 (1996).
9.
Mikhailov S.N., Fomitcheva M.V., Efimtseva E.V., Ermolinsky B.S., Meshkov S.V.,
Esipov D.S., Boldyreva E.F., Korobko V.G. Oligodeoxyribonucleotides containing 1--Dglucopyranosylthymine: synthesis and substrate properties. Nucleosides & Nucleotides,
V.15, No 10, 1619-1634 (1996).
IV
10.
M.G. Brevnov, O.M. Gritsenko, S.N.Mikhailov, E.V. Efimtseva, Ermolinsky B.S., A.Van
Aerschot, P. Herdewijn, A.V.Repyk, E.S. Gromova. DNA duplexes with reactive dialdehyde
groups as novel reagents for cross-linking to restriction-modification enzymes Nucleic Acids
Research, Vol.25,No.16., 3302-3309 (1997)
11.
V.L. Tunitskaya, E.E.Rusakova, N.Sh.Padyukova, Ermolinsky B.S., A.A.Chernyi,
S.N.Kochetkov, Yu.P. Lysov, S.N.Mikhailov. Substrate properties of C`-methyl UTP
derivatives in T7 RNA polymerase reactions. Evidence for N-type NTP conformation. FEBS
letters 400 , 1997, 263-266
12.
Efimtseva E.V., Victorova L.S., Rodionov A.A., Ermolinsky B.S., Fomitcheva M.V.,
Mikhailov S.N., Oivanen M., Van Aerschot A., Herdewijn P. Disaccharide nucleosides and
their enzymatic and chemical incorporation into oligonucleotides. Nucleosides &
Nucleotides,
17, 1681-1684, (1998)
13.
Tunitskaya V.L., Rusakova E.E., Memelova L.V., Kochetkov S.N., Van Aerschot A.,
Herdewijn P., Efimtseva E.V., Ermolinsky B.S., Mikhailov S. The mapping of T7 RNA
polymerase active site with novel reagents - oligonucleotides with reactive dialdehyde
groups. FEBS Lett., 442, 20-24, (1999).
14.
Mikhailov S.N., Rodionov A.A., Efimtseva E.V., Ermolinsky B.S., Fomitcheva M.V.,
Padyukova N.Sh., Rothenbacher K., Lescrinier E., Herdewijn P. Studies on disaccharide
nucleoside synthesis. Mechanism of the formation of trisaccharide purine nucleosides.
Nucleosides & Nucleotides 18, 691-692, (1999)
15.
Mikhailov S.N., Efimtseva E.V. Ermolinsky B.S., Brevnov M.G., Gritsenko O.M.,
Gromova E.S., Van Aerschot A., Herdewijn P. Oligonucleotides with reactive dialdehyde
groups as novel affinity reagents. Nucleosides & Nucleotides, 18, 1469-1470, (1999).
16.
Padyukova N.Sh., Dixon H.B.F., Efimtseva E.V., Ermolinsky B.S., Mikhailov S.N.,
Karpeisky M.Ya. Synthesis and properties of novel NTP derivatives. Nucleosides &
Nucleotides 18, 1013-1014, (1999).
V
17.
Rusakova E., Tunitskaya V., Memelova L., Kochetkov S., Ermolinsky B., Mikhailov S.
Localization of the initiation site in T7 RNA polymerase by affinity labeling. Nucleosides &
Nucleotides 18, 1359-1360, (1999).
18.
B.S.Ermolisky, and S.N.Mikhailov. Periodate oxidation in chemistry of nucleic acids:
Dialdehyde derivatives of nucleosides, nucleotides and oligonucleotides (Review). Russian
Journal of Bioorganic Chemistry 2000, 26 (7):429-449.
19.
O.I.Andreeva, A.S.Golubeva, S.N.Kochetkov, A.Van Aershot, P.Herdewjin, E.V.
Efimtseva, B.S.Ermolisky, and S.N.Mikhailov. An additional 2`-ribofuranose residue at a
specific position of DNA primer prevents its elongation by HIV-1 reverse transcriptase.
Bioorg. Med.Chem.Lett.12:681,(2002).
20.
O.M. Gritsenko, E.V. Koudan, S.N. Mikhailov, B.S. Ermolinsky, A.Van Aershot, P.
Herdewjin, E.S. Gromova. Affinity modification of EcoRII DNA methyltransferase by the
dialdehyde-substituted DNA duplexes: mapping the enzyme region that interacts with DNA.
Nucleosides Nucleotides Nucleic Acids. 2002 Nov-Dec;21(11-12):753-64.
21.
Ermolinsky BS, Efimtseva EV, Alexeev CS, Mikhailov SN, Balzarini J, De Clercq E.
Synopsis of current work. Dinucleoside monophosphates containing AZT and 1methyladenosine or 7-methylguanosine. Nucleosides Nucleotides Nucleic Acids. 2003 MayAug;22(5-8):853-5.
22.
Mikhailov SN, Efimtseva EV, Ermolinsky BS, Bobkov GV, Andreeva OI, Golubeva AS,
Kochetkov SN, Van Aerschot A, Schepers G, Herdewijn P. Oligonucleotides containing
disaccharide nucleosides: synthesis, physicochemical, and substrate properties. Nucleosides
Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1117-8
23.
Golubeva AS, Ermolinsky BS, Efimtseva EV, Tunitskaya VL, van Aerschot A, Herdewijn
P, Mikhailov SN, Kochetkov SN. Interaction of HIV-1 reverse transcriptase with modified
oligonucleotide primers containing 2'-O-beta-D-ribofuranosyladenosine. Biochemistry
(Mosc). 2004 Feb;69(2):130-6.
24.
Pacheco LF, Hernandez, E.F, Arshadmansab, M.F, Francisco, S., Willis, M., Ermolinsky,
B., Zarei, M.M, Knaus, H-G., Garrido Sanabria, E.R. Down-regulation of BK channel
expression in the pilocarpine model of temporal lobe epilepsy. Brain Research 2008, 1200:
116-131.
VI
25.
Ermolinsky B, Pacheco LF, Arshadmansab MF, Garrido-Sanabria ER. Differential changes in
mGluR2 and mGluR3 gene expression following pilocarpine-induced status epilepticus: A
comparative real-time PCR analysis. Brain Research 2008, 1226:173-180.
26.
Emilio R. Garrido-Sanabria, Luis F. Pacheco Otalora, Massoud F. Arshadmansab, Berenice
Herrera, Sebastian Francisco, Boris S. Ermolinsky. Impaired expression and function of
group II metabotropic glutamate receptors in pilocarpine-treated chronically epileptic rats.
Brain Research 2008, 1240: 165-176.
27.
Boris Ermolinsky, Massoud F Arshadmansab; Luis F Pacheco, Masoud M Zarei. Deficit
of Kcnma1 mRNA expression in the dentate gyrus of epileptic rats. Neuroreport 2008
19(13):1291-4.
28.
Guevara JG Jr, Prashad N, Ermolinsky B, Gaubatz JW, Kang D, Schwarzbach AE, Loose
DS, Guevara NV. Apo B100 similarities to viral proteins suggest basis for LDL-DNA
binding and transfection capacity. J Lipid Res. 2010 Jul;51(7):1704-18.
29.
Oliveira MS, Skinner F, Arshadmansab MF, Garcia I, Mello CF, Knaus HG, Ermolinsky
BS, Otalora LF, Garrido-Sanabria ER. Altered expression and function of small-conductance
(SK) Ca2+-activated K+ channels in pilocarpine-treated epileptic rats. Brain Res. 2010 Jun 8.
[Epub ahead of print]
30.
Ermolinsky BS, Skinner F, Garcia I, Arshadmansab MF, Otalora LF, Zarei MM, GarridoSanabria ER. Upregulation of STREX splice variant of the large conductance Ca2+-activated
potassium (BK) channel in a rat model of mesial temporal lobe epilepsy. Neurosci Res. 2011
Jan 69(1): 73-80.
31.
Pacheco Otalora LF, Skinner F, Oliveira MS, Farrell B, Arshadmansab MF, Pandari T,
Garcia I, Robles L, Rosas G, Mello CF, Ermolinsky BS, Garrido-Sanabria ER. Chronic
deficit in the expression of voltage-gated potassium channel Kv3.4 subunit in the
hippocampus of pilocarpine-treated epileptic rats. Brain Res. 2010 Oct 21.
VII