Julie Martone EN

Julie MARTONE
Personal information:
First Name:
Surname:
Nationality:
Date & place of birth:
Personal Address:
Laboratory Address:
Julie
Martone
Italian/French
April 22, 1978 Nice (France)
Via Angelo Poliziano 80, 00184 Rome- Italy
Dept. of Biology and Biotechnology "C. Darwin"
University of Rome “Sapienza”
P.le A. Moro 5, 00185 Roma, Italy
Tel. +39 6 49912210
Fax +39 6 49912500
mailto:: [email protected]
Speaks fluent French and English
Current Employment
Postdoctoral fellowship, University of Rome "Sapienza"
Field of interest
Molecular biology. Non coding RNA, miRNA, miRNA as serum biomarkers, neuromuscolar
disorders, nutritional genomics, GMO
Academic qualifications/Research experience
Jan. ’12 – Oct. ’14 : Postdoctoral fellowship, University of Rome "Sapienza"
Project: “Role of small non coding RNAs in Duchenne Muscular Dystrophy”
Jan. ’10 – Dec. ’11 : Postdoctoral fellowship, University of Rome "Sapienza"
Project: “Role of small non coding RNAs in Duchenne Muscular Dystrophy”
Apr. '10: Member of Italian National Order of Biologists
1
Nov. '07 - Ott. '09: Postdoctoral fellowship at the Genetics and Molecular Biology Department,
University of Rome "Sapienza"
Project: “Role of miRNAs in Duchenne Muscular Dystrophy”
Nov. '06 - Ott. '07: Contract with P. Vittorioso at the Genetics and Molecular Biology Department,
University of Rome "Sapienza"
Feb. '07: PhD in Genetics and Molecular Biology, Department of Genetics and Molecular Biology,
University of Rome “Sapienza”
Nov. '03 - Ott. '06: PhD Course in Genetics and Molecular Biology, University of Rome
“Sapienza”. PhD student in the laboratories of Prof. P. Costantino. Responsible scientist:
P.Vittorioso, PhD
PhD Thesis Project: “The DAG1 transcription factor from Arabidopsis thaliana: identification of
the targets and study of its transcriptional/post-transcriptional regulation”
March '05 - Apr. '05: Stage in the laboratories of Jan Traas, PhD, INRA-Versailles (France)
July '03: Laurea (MS) Degree in Biology (110/110 e lode), "Summa cum laude", at the University
of Rome “Sapienza” (Italy)
Thesis project: "The DOF transcription factors DAG1 and DAG2 of Arabidopsis thaliana:
biochemical and molecular characterization"
Nov.'01 - July'03: Student in the laboratories of Prof. P. Costantino, at the Genetics and Molecular
Biology Department, University of Rome “Sapienza".
Sep.'99 - March '00: Stage at the “Observatoire Océanologique de Villefranche sur mer” (Ecole
interne de l’Université Pierre et Marie Curie, Paris- France) in the laboratories of Prof. P. Mayzoud
Project: “Dynamic of a Paraeuchaeta antarctica population”
Teaching experience
'06 -'07: assistant ("cultore della materia") in Genetic Engineering course at the University of
Rome "Sapienza"
Lab skills
General Molecular biology tecniques: PCR, RT-PCR, qPCR, cloning tecniques, restriction map
analysis, DNA sequencing, ultracentrifugation, nucleic acid radiolabelling -32P, 35S-, hybridisation,
Southern, Northern, 5'-3' RACE assays, bioinformatic analyses, EMSA assays, in vitro transcription
2
and translation, in situ hybridization, deep sequencing, microarray (mRNA and miRNA)
Basic methods in microbiology
Arabidopsis tecniques: in vitro and in vivo growth, transformation, phenotypical analysis
Imaging analysis: Confocal and optical microscope (GFP, Nomarsky)
Biochemical tecniques: Protein extraction and purification from plants and mammalian
cells/tissues, SDS-PAGE, Western Blot analysis (Colorimetric and Chemiluminescent detection),
pull-down assays, immunoprecipitation, enzymatic assays of products of "reporter genes" (GUS,
luciferase)
Computer methods for molecular biology: software and on-line packages for DNA and protein
sequence analysis, database similarity searches, RNA structure prediction
Mammalian cell-culture techniques: transfection (plasmids, oligos), RNA-interference
technique, luciferase assay, lentivirus preparation and infection
PATENTS
Application #PCT109052: Serum miRNAs as biomarkers for tissue degeneration
Application #PCT109053: Oligonucleotide sequences complementary to DMD-3’UTR increase
dystrophin rescue in Duchenne Muscular Dystrophy gene therapies.
Application #PCT/EP2011/059435 (Application -Filing date 08.06.2011 – Publication date 15-
12-11): Modified snRNAs for use in therapy
PUBLICATIONS
Spallotta F., Tardivo S., Nanni S., Rosati J.D., Straino S., Mai A., Vecellio M., Valente S., Capogrossi
M.C., Farsetti A., Martone J., Bozzoni I., Pontecorvi A., Gaetano C., Colussi C. (2013) Detrimental
effect of class-selective histone deacetylase inhibitors during tissue regeneration following hindlimb
ischemia. J Biol Chem. 288(32):22915-29.
Martone J., Cazzella V., Pinnarò C., Santini T., Twayana S.S., Sthandier O., D'Amico A., Ricotti V.,
Bertini E., Muntoni F., Bozzoni I. (2012) Exon 45 skipping through U1-snRNA antisense molecules
3
recovers the Dys-nNOS pathway and muscle differentiation in human DMD myoblasts. Mol Ther.
20(11):2134-42.
Martone J., De Angelis F.G., Bozzoni I. (2012) U1 snRNA as an Effective Vector for Stable
Expression of Antisense Molecules and for the Inhibition of the Splicing Reaction. Methods Mol Biol.
867:239-57.
Cacchiarelli D., Legnini I., Martone J., Cazzella V., D’Amico A., Bertini E. and Bozzoni I. (2011)
miRNAs as serum biomarkers for Duchenne Muscular Dystrophy . EMBO Mol. Med. 3(5):258-65.
Cacchiarelli D., Incitti T., Martone J., Cesana M., Cazzella V., Santini T., Sthandier O., Bozzoni I.
(2011) miR-31 modulates dystrophin expression: new implications for Duchenne muscular dystrophy
therapy. EMBO Rep. 12(2):136-41.
Martone J., Cacchiarelli D., Girardi E., Cesana M., Incitti T., Morlando M., Nicoletti C., Santini T.,
Sthandier O., Barberi L., Auricchio A., Musarò A., Bozzoni I. (2010) miRNAs involved in molecular
circuitries relevant for the DMD pathogenesis are controlled by the dystrophin/nNOS pathway. Cell
Metab. 12(4):341-51.
Gabriele S., Rizza A., Martone J., Circelli P., Costantino P., and Paola Vittorioso (2010). The Dof
protein DAG1 mediates PIL5 activity on seed germination by negatively regulating GA biosynthetic
gene AtGA3ox1. Plant J. 61(2):312-23.
CONFERENCES AND COURSES
•
X Conferenza Internazionale sulla Distrofia Muscolare Duchenne e Becker 17-19 febbraio
2012 Roma - Ergife Palace Hotel
•
SIROCCO Annual Meeting “RNA silencing in Europe”, 5-7 September 2011 Magdalene
College, Cambridge (UK)
•
Martone J., Cacchiarelli D., Legnini I., Bozzoni I. “miRNAs as serum biomarkers for Duchenne
Muscular Dystrophy “ XVI Scientific convention Theleton, March 2011 Riva del Garda (TN)
Italy (oral presentation).
4
•
Martone J., Cacchiarelli D., Cazzella V., Cesana M., Incitti T., Pinnarò C., Legnini I., Morlando
M., Bozzoni I. “Role of miRNA in Duchenne Muscular Dystrophy”. 4th international congress of
myology, May 2011 Lille Grand Palais -France (oral presentation).
•
Martone J., “Il ruolo dei microRNA nella DMD”. Conferenza Internazionale sulla Distrofia
muscolare Duchenne e Becker, Roma (IT) 18 – 20 febbraio 2011 (oral presentation).
•
ICE Meeting 2010 for DMD preclinical research program "Towards clinical trials for DMD using
gene and cell therapies combined with exon skipping" - January 23rd, 2010 Principality of
Monaco (oral presentation).
•
Cacchiareli D., Martone J.Cesana M., Morlando M., Girardi E., Incitti T. and Bozzoni I. “miRNAs
involved in molecular circuitries relevant for the DMD pathogenesis are controlled by the
dystrophin/nNOS pathway” The Non-Coding Genome, October 2011 EMBL Advanced
Training Centre, Heidelberg, (Germany).
• Cacchiareli D., Martone J.Cesana M., Morlando M., Girardi E., Incitti T. and Bozzoni I. “miRNAs
involved in molecular circuitries relevant for the DMD pathogenesis are controlled by the
dystrophin/nNOS pathway” SIROCCO Annual Meeting 11-13 October 2010 EMBL Advanced
Training Centre Heidelberg, (Germany).
•
Martone J., Cacchiareli D., Cesana M., Girardi E., Incitti T., Sthandier O., Nicoletti C. and
Bozzoni I. “Global MicroRNAs profiling and Duchenne muscular dystrophy”. TREAT-NMD
Conference Brussels, Belgium, 17-19 November 2009.
•
SIROCCO sRNA Cloning Course 21-24 September 2009. Plant Sciences Department,
University of Cambridge.
•
De Angelis FG, Incitti T, Sthandier O, Cazzella V, Pinnarò C, Cacchiarelli D, Cesana M, Girardi E,
Martone J & Bozzoni I. “RNA-based gene therapy of duchenne muscular dystrophy: production
and validation of antisense constructs able to induce exon skipping in different DMD mutations”.
XV Scientific Convention Telethon, Riva del Garda (TN), Italy, 9-11 Marzo 2009.
•
Martone J., Cacchiareli D., Cesana M., Girardi E., Incitti T. and Bozzoni I "Global miRNAs
profiling and Duchenne Muscular Dystrophy" SIROCCO Annual Meeting 10-12 December 2008
Wellcome Trust Conference Centre, Hinxton UK.
•
SIROCCO Annual Meeting 19-21 November 2007 Harnack House, Berlin.
•
Erika Girardi, Monica Ballarino, Julie Martone, Marcella Cesana, Irene Bozzoni. “The microRNA
transcription machinery: analysis of costitutive and tissue-specific microRNAs”. EMBO
5
Conference Series (1°), The molecular and cellular mechanisms regulating skeletal muscle
development and regeneration. Sant Feliu de Guixols, Spain 24-29 September 2008.
•
S. Gabriele, J. Martone, L. Corso, G. Ragone, P. Costantino and P. Vittorioso “The Arabidopsis
Dof trancription factor DAG1 is involved in PhyB- and GA-mediated seed germination” 18th
International Conference on Arabidopsis Research, Beijing, China June 20-23, 2007
•
J. Martone, S. Gabriele, G. Ragone, P. Circelli, P. Costantino and P. Vittorioso “Study of the
transcriptional/post-transcriptional regulation of the Arabidopsis DAG1 gene 8th Annual Congress
FISV, September 2006, Riva del Garda (IT)
•
S. Gabriele, J. Martone, G. Ragone, P. Costantino and P. Vittorioso “Mode of action of DAG1
and DAG2 Arabidopsis TFs involved in phytocrome and gibberellins-mediated seed germination”
7th Annual Congress FISV, 22-25 September 2005, Riva del Garda (IT)
•
J. Martone, S. Gabriele, G. Ragone, P. Costantino, P. Vittorioso “Intercellular transport and mode
of action of DAG1, a Dof transcription factor involved in PhyB-mediated seed germination”
Summer School in Plant Development, Società Italiana di Fisiologia Vegetale, June 8-11 2005,
Maratea (IT) (oral presentation)
•
Martone J., Berretti M., Gabriele S., Ragone G.*, P. Costantino, and Vittorioso P. “DAG1 and
DAG2: two Arabidopsis transcription factors that play a maternal role in controlling seed
germination”, 2nd EPSO Conference, 10-14 October 2004, Ischia (IT)
•
Martone J., Berretti M., Gabriele S., Ragone G., P. Costantino, and Vittorioso P. “DAG1 and
DAG2: two Arabidopsis transcription factors that play a maternal role in controlling seed
germination” 15th International Conference on Arabidopsis Research, Berlin, Germany, July
2004
•
M. Berretti, J. Martone, S. Gabriele, G. Ragone, P. Costantino e P. Vittorioso “DAG1 e DAG2:
due proteine “materne” coinvolte nella germinazione dei semi di Arabidopsis thaliana”
6th
Annual Congress FISV 2004, Riva del Garda
•
Vittorioso P., Papi M., Martone J., Berretti M., Gualberti G., and P. Costantino "DAG1 and
DAG2, two highly related transcription factors involved in seed germination, act on the same target
genes with opposite effects" 7° International Congress of Plant Molecular Biology, Barcelona,
Spain, June 2003
01/8/2014
6