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2143 Advances in Environmental Biology, 6(7): 2143-2150, 2012 ISSN 1995-0756 This is a refereed journal and all articles are professionally screened and reviewed ORIGINAL ARTICLE A Review on Anaerobic Digestion, Bio-reactor and Nitrogen Removal from Wastewater and Landfill Leachate by Bio-reactor 1 Amin Mojiri, 1Hamidi Abdul Aziz, 1Nastaein Qamaruz Zaman and 2Shuokr Qarani Aziz 1 School of Civil Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia 2 Department of Civil Engineering, College of Engineering, University of Salahaddin–Erbil, Iraq Amin Mojiri, Hamidi Abdul Aziz, Nastaein Qamaruz Zaman and Shuokr Qarani Aziz; A Review on Anaerobic Digestion, Bio-reactor and Nitrogen Removal from Wastewater and Landfill Leachate by Bio-reactor ABSTRACT The nitrogen removal from wastewater and landfill leachate has become a vital part of the overall treatment process because nitrogen compounds have the significant impact on the environment. Generally, biological nitrogen removal is used for nitrogen elimination from wastewater. In the last years, newly advanced anaerobic reactor systems such as up flow anaerobic sludge blanket (UASB), anaerobic filter (AF), anaerobic fluidized bed (FB), anaerobic sequencing batch reactor (AnSBR) and other anaerobic reactors have been used for the treatment of low strength wastewater, landfill leachate and, etc. The aims of this study were the review on the anaerobic digestion, some kinds of bio-reactors and nitrogen removal from wastewater and landfill leachate by bio-reactor. Many investigations showed that the bio-reactor and anaerobic treatments could be reducing nitrogen from wastewater and landfill leachate. Additionally, many studies also were reported some environmental factors such as pH, temperature, ammonia, alkalinity and nutrients affecting on anaerobic digestion. Key words: Anaerobic digestion, bio-reactor, nitrogen removal, wastewater, landfill leachate Introduction One of the elements of concern in wastewater is nitrogen, especially since the use of synthetic nitrogen fertilizers produced from atmospheric N2 by the Haber-Bosch process has increased tenfold over the last 40 years. The human contribution to nitrogen pollutions, especially in the form of urine, is ever increasing in view of the growing world populations. Discharge of this nitrogen into the natural waters can lead to eutrophication and oxygen depletion [11]. The nitrogen removal from wastewater has become a vital part of the overall treatment process because nitrogen compounds have significant impacts on the environment. Conventionally, removal of nitrogen is performed by means of a two step process. First, NH4+ is oxidized to nitrite (NO2-) or nitrate (NO3-) by an autotrophic nitrification process, and this is subsequently reduced to nitrogen gas by a heterotrophic nitrification process. However, some wastewaters are rich in NH4+ but poor in biodegradable organic carbon; these wastewaters are less suitable for biological nitrogen removal via the conventional nitrification-denitrification processes [18]. One of the common and economical methods in wastewater treatment is biological processes. The main objective of biological treatment is to convert organic materials into other products using microorganisms. Generally, biological nitrogen removal is used for nitrogen elimination from wastewaters. Although NO2- or NO3- can be present, ammonia (NH3-N) is abundant in many wastewater streams. NH3-N removal is often achieved using nitrification/denitrification processes. In such systems, nitrifying bacteria oxidize NH3-N to NO3under oxic conditions, and NO3- is subsequently or simultaneously reduced to nitrogen gas under anoxic conditions [20]. Nitrogen removal is normally realized by sequentially alternating between oxic and anoxic situations or by the creation of separated zones with suitable conditions for nitrification and denitrification, respectively. Alternatively, high rates of simultaneous nitrification and denitrification (SND) can be achieved, in activated sludge and biofilm type systems alike, at operational conditions where both oxic and anoxic micro-environments are present. Nitrification can occur at the liquid/biomass Corresponding Author Amin Mojiri, School of Civil Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia E-mail: [email protected] 2144 Adv. Environ. Biol., 6(7): 2143-2150, 2012 interface, while denitrification of NO3- (or NO2-) may be found in deeper sub-surface biomass zones [4]. Biological nitrogen removal proceeds slowly because the microorganisms responsible for the elimination reactions grow slowly. In addition, the operational control of aerobic and anaerobic conditions needed for nitrification and denitrification, respectively, can be difficult. To cope with these problems, various kinds of bioreactors have been studied for enhancing the efficiency of nitrogen removal. Examples of enhanced processes include the combined nitrification and denitrification, immobilization of bacteria on polymeric gel beads in a moving bed biofilm reactor, and the formation of bacterial film on the surface of rotating disks or other packing in a moving bed biofilm reactor or aeration tank [12]. The objectives of this study were the review of the some kinds of bio-reactor and nitrogen removal from wastewater and landfill leachate by bio-reactor. Anaerobic digestion (AD): Anaerobic treatment represents a sustainable and appropriate wastewater treatment system for developing countries and already is becoming an accepted simple and cost-effective technology for the treatment of a variety of wastewaters [1]. AD is a biological process that happens naturally when bacteria breaks down organic matter in environments with little or no oxygen. It is effectively a controlled and enclosed version of the anaerobic breakdown of organic waste in landfill which releases methane [6]. AD of industrial wastewater is commonly used all over the world, not only as a pollution control tool but also for energy recovery as methane [22]. The AD process takes place in an airtight container, known as a digester. The first stage of AD is a chemical reaction called hydrolysis, where complex organic molecules are broken down into simple sugars, amino acids, and fatty acids with the addition of hydroxyl groups. This is followed by three biological processes: Fig. 1: The three distinct temperature ranges [25]. Acidogenesis - further broken down by acidogenic bacteria by into simpler molecules, volatile fatty acids (VFAs) occurs, producing ammonia, CO2 and hydrogen sulfide as byproducts. Acetogenesis - the simple molecules from acidogenesis are further digested by bacteria called acetogens to produce CO2, hydrogen and mainly acetic acid. Methanogenesis - methane, CO2 and water are produced by bacteria called methanogens. The pH level should be kept between 5.5-8.5 and the temperature between 30-60°C, in order to maximize digestion rates [6]. Some environmental factors affecting on AD: Temperature: There are three distinct temperature ranges associated with microbial growth in most of the biological processes namely: psycrophilic range between 4 and approximately 15 °C, Mesophilic range between 20 and approximately 40 °C and thermophilic range between 45 and 70 °C and above. In each of these ranges, where microbial growth is possible, within these ranges three temperatures values are usually used to characterize the growth of the microorganism species namely [7]; Minimum temperature, below which growth is not possible Optimum temperature, in which growth is maximum Maximum temperature, above which growth is not possible Temperature effects on kinetic formulation: specific microbial growth rate, half-saturation constant, and inhibition constants were presumed to be exponential equation [3]: F (T) = eθ (T-To) (1) Where θ = temperature coefficient in the mesophilic (30−40°C) and thermophilic (50−60°C) temperature range. 2145 Adv. Environ. Biol., 6(7): 2143-2150, 2012 pH and alkalinity requirements: The optimum pH for methane fermentation is between 7 and 8, but the methane bacterio generally are not harmed unless the pH drops below about 6, the lower the pH the shorter the time for a given decrease in activity. For this reason, it is essential that the pH be maintained above 6 at all time. When the bicarbonate alkalinity drops below about 500 mg.L-1, and with the normal percentage of carbon dioxide in the digester gas, the pH will drop dangerously close to 6. When digesters become unbalanced, the volatile acid concentration increases destroying the bicarbonate alkalinity [8]. Low pH inhibits the process, for an enhanced degradation rate, a neutral pH is recommended [25]. The pH is an important factor for keeping functional anaerobic digestion. A typical pH is in the range of 6.5-7.6. The accumulation of intermediate acids leads to pH drop during fermentation. In order to maintain stable operation, it is necessary to add bicarbonate or carbonate as an alkalinity buffer to neutralize volatile fatty acids and carbon dioxide [3]. Alkalinity is an important parameter in anaerobic digestion; it is a measure of chemical buffering capacity of the aqueous solution. Bicarbonate, hydrogen sulphide, dihydrogen phosphate and ammonia are the compounds that provide a significant buffering capacity in the useful region around pH 7. The other compounds such as VFAs and ammonia also contribute to the alkalinity. The alkalinity is generated from the decomposition of organic compounds during the anaerobic digestion process [7]. High VFA concentration: Fermentative reactions stopped at a VFA concentration of 13,000 mg/L accompanied by a low pH of 5. Propionic acid at a concentration of more than 1000 mg.L-1 is considered as toxic and can cause digester failure [25]. Microbiology of AD: Although anaerobic digestion is generally considered a two-phase process, it can be subdivided into various metabolic pathway, with the participation of several microbial groups is illustrated in Fig. 1 [25]. Fig. 2: Metabolic pathways and microbial groups involved in AD [16 & 7]. concentration of 1500 mg/L which leads to increase the pH up to 8.5 which is toxic to methanogens [25]. NH3-N: Ammonia inhibition occurred at NH3-N 2146 Adv. Environ. Biol., 6(7): 2143-2150, 2012 Table 1: Effect of NH3-N on AD process [25]. NH4-N (mg L-1) 50-200 200-1000 1500-3000 Above 3000 EFFECT Beneficial No adverse effect Inhibitory at pH over 7.4-7.6 Toxic Nutrients requirements: All organisms need essential ingredients for their growth, viz. macronutrients and micronutrients. The deficiency of these nutrients will negatively affect the growth of these organisms. Even though the requirement of these elements is in very low concentration, but play very important role in the growth and performance of the microorganisms [7]. Nitrogen and Phosphorus, The nitrogen requirement for anaerobic treatment is only a small fraction of that required by aerobic process and the phosphorus requirement is approximately 15% of nitrogen requirement. Trace nutrient requirements, the lack of understanding of trace nutrients requirements of methanogens has been a serious hindrance to the commercialization of anaerobic biotechnology. Four elements including iron, cobalt, nickel and sulfide have been shown to be essential for methanogens to convert acetate to methane [8]. Some Important treatment: calculations for while anaerobic digestion of which the liquid has to stay within the digester until degradation. The HRT can be calculated as follows [15]. (3) Where, HRT= Hydraulic retention time (days) OLR= Organic loading rate (kg-COD/L.day) CODin= Influent COD (kg-COD/L) The flow rate: The HRT and flow rate examine the exact influent stream from feed inlet to the outlet. Normally, flow rate is controlled by means of a peristaltic pump with corresponding tube hosing of different diameter. The flow rate is designed according to the working volume of the reactor. The flow rate can be calculated as follows [15], (4) anaerobic The F/M ratio: The F/M ratio would simply be the digester loading divided by the concentration of volatile suspended solid (biomass) in the digester (kg-COD/kg-VSS.day). For any given loading, efficiency can be improved by lowering the F/M ratio and increasing the concentration of biomass in the digester. Also for given biomass concentration within the digester, the efficiency can be improved by decreasing the loading. The F/M can be calculated as follows [15], (2) Where, Organic loading rate= COD of the influent stream (kg-COD/L.day) Volatile solid= Volatile suspended solid concentration in the reactor (kg-VSS/L) F/M= kg-COD/kg-VSS.day The hydraulic retention time (HRT): The HRT calculation before proceeding experiments is also an important process control parameters. It shows the total time required by the liquid to degrade. The HRT plays an important role Where, Q= Flow rate of influence stream (L/day) Vw= Working volume of the reactor (L) HRT= Hydraulic retention time (days) A new and promising trend in wastewater and solid waste management is using a bio-reactor. In the last few years, newly advanced anaerobic reactor systems, such as UASB, anaerobic filter (AF), anaerobic fluidised bed (FB), anaerobic sequencing batch reactor (AnSBR) and other modifications of anaerobic reactors have also been used for the treatment of low strength wastewater [Bodik et al., 2002] landfill leachate and etc. The upflow anaerobic sludge blanket (UASB): The UASB reactor was developed in the Netherlands in the early 1970s [16]. This reactor, as originally proposed by Lettinga, was one of the earliest systems in which development of a granular biomass was observed. As the result of the excellent settling characteristics of this granular biomass and the presence of a specially designed three-phase (biogas, water and biomass) separator device in the upper part of the UASB-reactor, excellent sludge retention is assured in this reactor system. The major disadvantage of the UASB process comprises, although merely in those cases where proper seed 2147 Adv. Environ. Biol., 6(7): 2143-2150, 2012 sludge is not available in sufficient quantities, the relatively long start-up period. During the initial phase of the (first) start-up process of a UASB-reactor, inoculated with flocculent seed sludge, a significant washout of sludge generally will manifest and therefore the first reactor start-up would highly benefit from skilled operation [19]. The UASB reactor is the most successfully used as high rate anaerobic treatment system and it becomes possible high volumetric loadings at short retention times and at high temperatures. The performance of the UASB system is limited by slow hydrolysis of entrapped solids especially at low temperatures and a two-phase system can be operated to provide optimal conditions for the microorganisms for greater efficiency in digestion in the treatment of domestic wastewater [1]. The UASB reactor (in sequel as UASBR) consists of a sludge bed in the lower part and a three phase separator (g-l-s separator) in the upper part of the reactor [9]. Fig. 3: UASB Reactor Schematic The Upflow anaerobic filter (UAF): Anaerobic hybrid reactor (AHR): Anaerobic filters have been used successfully for the treatment of dilute wastewater with chemical oxygen demand (COD) removal efficiencies between 60 and 80% depending on the HRT used [1]. Padilla-Gasca and López reported the UAF presents advantages over other anaerobic treatment systems such as the UASB or the expanded bed reactor. The UAF maintains the biomass immobilized over a fixed support, which allows the maintenance of high Cellular Retention Times (CRT) in spite of operating at low HRT. Also, the UAF tolerates small variable Organic Loading Rate (OLR) and pH. These features enable a reduction in the reactor’s dimensions and, as a consequence, capital investment, but above all, make it easier to operate. The essential features of the anaerobic filter design was listed by Switzenbaum [21] as: a distributor in the bottom of the column, a media support structure, inert packing material, a free board above the packing material, effluent draw-off and optional features such as, recycle facilities, backwashing facilities or a sedimentation zone below the packing material [1]. The anaerobic hybrid reactor combining the sludge blanket in the lower part and filter in the upper part has been reported to promote the advantages of both UASB and upflow filter, while minimizing their limitations [13]. The AHR is a combination of the UASBR and the anaerobic filter. A layer of biomass carrier is situated in the upper part of the AHR instead of the g-l-s separator. This layer separates the bubbles of the biogas from the biomass and acts as a support material for the biomass growth as well. The layer even has a notable efficiency as a suspended solids (SS) separator [9]. AnSBR (Anaerobic sequencing batch reactor): An AnSBR process is one of the novel and promising high-rate anaerobic processes and has been used for treating organic wastewaters.7–10 Sequencing batch reactor processes offer distinct advantages when compared with continuous processes, including a high degree of process 2148 Adv. Environ. Biol., 6(7): 2143-2150, 2012 flexibility and no requirement for a separate clarifier. This process repeats a cycle that includes feeding, reaction, settling and withdrawal steps in a single reactor [17]. Dague et al. [5] studied anaerobic treatment of dilute wastewater using the laboratory AnSBR. The results showed that the AnSBR process was capable of achieving in excess of 90% soluble COD and 5-day biochemical oxygen demand (BOD5) removal at temperatures of 25 °C and 20 °C at HRT in range 6–24 h. At the low temperature of 5 °C and the 6 h HRT, soluble COD and BOD5 removals were 62% and75%, respectively [2]. Fig. 4: Schematic diagram of the UAF-reactor [19]. Nitrogen removal from wastewater by bio-reactor: Lv et al. [18] studied autotrophic nitrogen removal discovered in suspended nitritation system. This study revealed that nitritation, Anammox and autotrophic denitrification were responsible for the nitrogen removal. The NO3- production was caused by the coaction of nitrite-oxidizing bacteria and AnAOB (anaerobic ammonium oxidationbacteria). Lackner et al. [14] investigated Heterotrophic activity compromises autotrophic nitrogen removal in membrane-aerated biofilms: Results of a modeling study. These results clearly indicate the importance of heterotrophic activity in autotrophic N removal biofilms, especially in counter-diffusion systems where they may compromise N removal capacity. Clifford et al. [4] investigated Nitrogen dynamics and removal in a horizontal flow biofilm reactor for wastewater treatment. These results can be used to optimise horizontal flow biofilm reactor (HFBR) reactor design. The HFBR technology could provide an alternative, low maintenance, economically efficient system for carbon and nitrogen removal for low flow wastewater discharges. Yu and Zhou [24] studied nitrogen removal efficiency of an A2/O bio-reactor treating domestic sewage mixed with landfill leachate and fecal sewage. These results showed that: the optimal volume ratio of landfill leachate, fecal sewage and urban wastewater in the A2/O process was 1:3.75:1000. The average removal efficiency of NH3-N, total nitrogen and COD can reach 96%, 61% and 85% respectively under the conditions of HRT of 11h, dissolved oxygen of 3 mgL-1 the mixed-liquid return ratio (r) of 200% and sludge return ratio (r) of 80%. Sliekers et al. [20] studied completely autotrophic nitrogen removal over NO2- in one single reactor. These methods showed that during steady state, anaerobic ammonium-oxidizing bacteria remained present and active. In the reactor, no aerobic nitrite-oxidizers were detected. The denitrifying potential of the biomass was below the detection limit. NH3-N was mainly converted to N2 (85%) and the remainder (15%) was recovered as NO3-.N2O- production was negligible (less than 0.1%). Addition of an external carbon source was not needed to realize the autotrophic denitrification to N2. Nitrogen removal bio-reactor: from landfill leachate by He et al. [10] investigated characteristics of the bioreactor landfill system using an anaerobic–aerobic process for nitrogen removal. After 56 days operation, the leachate total nitrogen and NH4 -N concentrations decreased to less than 200 mg/l in the bioreactor landfill system. The COD concentration was about 200 mg/l with less than 8 mg/l BOD5 in recycled leachate at the late stage. 2149 Adv. Environ. Biol., 6(7): 2143-2150, 2012 Tengrui et al. [23] investigated characteristics of nitrogen removal from old landfill leachate by sequencing batch biofilm reactor (SBBR). The results showed that after two months long period of domestication and one month period of stability, the NH3-N removal efficiency reached to 99% in the SBBR, at nitrogen loading rate 0.51 kg total nitrogen m-3 per day and HRT was 9 hrs, met to Chinese standards for discharge. Visvanthan et al. [26] investigated landfill leachate treatment using thermophilic membrane bioreactor. This result indicates that thermophilic system is highly suitable for COD and BOD5 removal especially at elevated organic loading. 5. 6. 7. 8. Conclusions: 9. AD plays an important role in wastewater treatment processes. It includes a series of biochemical processes by different microorganisms to degrade organic matter under anaerobic conditions. Methane, the digestion byproduct, is a rich source of renewable energy, which can help to replace fossil fuel to contribute to environmental conservation and sustainability. Therefore, AD is widely used as an attractive means for wastewater treatment around the world while more and more new process configurations are continuously being developed. Many studies were reported some environmental factors affecting anaerobic digestion such as pH, temperature, NH3-N, alkalinity and nutrients. Newly advanced anaerobic reactor systems, such as UASB, anaerobic filter AF, FB, AnSBR and other modifications of anaerobic reactors have also been used for the treatment of low strength wastewater. In literature, many investigations showed that the bio-reactor could be reducing nitrogen from wastewater and landfill leachate. 10. 11. 12. 13. References 14. 1. 2. 3. 4. Alptekin, E.E., 2008. Anaerobic Treatment of Dilute Wastewaters. 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