O A

1218
Advances in Environmental Biology, 5(6): 1218-1220, 2011
ISSN 1995-0756
This is a refereed journal and all articles are professionally screened and reviewed
ORIGINAL ARTICLE
Study on Blood Concentration of A, D vitamins and β-carotene in Dairy Cows affected
Heat Stress and effect on Calving to Conception Interval
1
1
2
DavasazTabrizi Afshin, 2Safarmashaei Saeid
Department of Clinical Sciences, Tabriz Branch, Islamic Azad University, Tabriz,Iran.
Tabriz Branch, Islamic Azad University, Young Researchers Club.
DavasazTabrizi Afshin,2Safarmashaei Saeid: Study on Blood Concentration of A, D vitamins and βcarotene in Dairy Cows affected Heat Stress and effect on Calving to Conception Interval
ABSTRACT
Theaim of this study was to investigate the roles of vitamin A, β-carotene and vitamin C in thermal stress
and its relationship with free days. Blood samples were prepared by vacuum tube from tail vein in thirty
Holstein dairy cattle of Tabriz farms, Blood sampling was conducted in summer under the temperature more
than +30Ec and as same manner in winter in -5Ec. Samples were centrifuged after clotting then their serum
was removed and stared in -20.The levels of vitamin A, β-carotene were measured by N-Hexane method and
level of vitamin C was measured by louri method. There is significant difference in results of every three
parameters in both groups (P<0.01).There is meaningful positive correlation among vitamin A, Beta carotene
and free days (P<0.01). Meanwhile, there is meaningful negative correlation among vitamin A, Beta carotene,
open days and vitamin C (P< 0.01).The results showed that the cows under heat stress in this region did not
indicate the signs of vitamin A and Betacarotene deficiency whereas vitamin C concentration was reduced
clearlyunder heat stress. It is seconded that its application has useful effects on prevention of heat stress
destructive effects in region dairy cows.
Key words: Heat stress, Dairy cow, Vit A, β-carotene, Vit C.
Introduction
Thermal heat stress causes problems in dairy
cows raising industry. It affects negatively on many
reproduction parameters. It can be referred to
reducing estrus period, increasing fetal mortality,
increasing probability of abortion and anestrous,
reduction in lactation and enhancement of interval
between calving and fertility [1,3,10,16]. The aim of
the present study was to describe theBlood
Concentration of A, D vitamins and β-carotene in
Dairy Cows affected Heat Stress and effect on
Calving to Conception Interval.
Material and method
Holstein from the dairy herd of the Tabriz area
(Center of East-Azerbaijan province) Farms were
used as experimental animals. A total number of 30
head of cows during winter season in temperature
loss than -5°C and 30 head during summer season in
temperature more than +30°C and these caws after
40days after calving were collected. Blood samples
from tail vein of every cattle by vacuum tube were
taken and after clotting this blood samples were
centrifuged and their serum for titration were
removed and in temperature of -20°C stored. After
these steps serums were used for A, D vitamins and
Beta carotene seral titration. Seral titration of A, D
vitamins and β-carotene were determined according
to Suzuki and Katoh [15] and Omaye et al., [11].
In present study diary cattle’s of the breed
Corresponding Author
DavasazTabrizi Afshin, Department of Clinical Sciences, Tabriz Branch, Islamic Azad University,
Tabriz,Iran.
E-mail: Davasaz1000@Yahoo; [email protected]; Tel: 00989144115045
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Adv. Environ. Biol., 5(6): 1218-1220, 2011
Results
In our study 30 head of diary cattle’s from farms
of Tabriz area were collected and seral titration of A,
D vitamins, β-carotene and number of open days
were calculated in seasons of winter and summer and
these amounts on following tables and figures has
been shown. Also from T-test and SPSS program
(version 14) for data analysis were used. P<0.05 was
considered significant.
Fig. 4: Average of Open daysin diary cattle’s in
summer and winter seasons.
Fig. 1: Average of blood concentration (mg/dl) of
Vit A in diary cattle’s in summer and
winter seasons.
According to results of this study, a significant
different between two sub studies groups were
observed. By attention to normal seral quantities of
vitamin A and β-carotene in group of under thermal
heat stress this was in normal range but this amount
in cattle’s of winter season group was loss than
normal range. Also in group of under thermal heat
stress amount of C vitamin was loss than normal
range but in group of winter season was normal.
Average of open days in group of winter season was
85day and this amount is normal but for group of
under thermal heat stress was 124 days that this
amount is not normal.
Conclusion:
Fig. 2: Average of blood concentration (mg/dl) of
β-carotene in diary cattle’s in summer and
winter seasons.
Fig. 3: Average of blood concentration (mg/dl) of
Vit C in diary cattle’s in summer and winter
seasons.
Heat stress cases oxidative stress and reduce the
seral titration of Vit C (is an antioxidant vitamin) in
pigs and poultry is one importance results of that [9,
14]. When produce of free radical is faster than
neutralization of them by antioxidative mechanisms,
at this time cases oxidative stress [9,13]. Therefor
possibility heat stress have effective role on oxidation
of antioxidative mechanisms. Most research on effect
of heat stress on oxidative condition of cattle’s has
been reported. In one study by Harmon et al the
effect of heat stress on reduce of antioxidative
activity of cattle plasma were reported, also reduce
in total concentration of Vit E in serum of cattle’s
under heat stress were observed[3], but in other study
were determined that heat stress have not effect on
concentration of α-tocopherol on cattle plasma [16].
Acid ascorbic is one importance antioxidant and
produce in liver of poultry and farm animals, but the
plasma titration of that reduce in mastitis [6,17]. In
some study the effect of heat stress on reduce of acid
ascorbic in pigs and poultry has been shown [4].
Blood levels of Vit C are dependent to produce and
use of acid ascorbic [2,7]. Also effect of heat stress
on reduce of glycogenolysis in diary cattle’s has been
reported [2,8]. In present study a significant different
in concentration of Vit C in cattle under heat stress
were observed and this subject is agree with results
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Adv. Environ. Biol., 5(6): 1218-1220, 2011
Table 1: Average of C, A Vitamins, β-carotene and open days in two groups of diary cattle’s
Parameter
unit
group
Number of samples
mean±SEM
Vit C
Mg/dl
1
30
0/005±0/153
2
30
0/004±0/315
Vit A
Mg/dl
1
30
1/28±25/94
2
30
1/84±19/84
Β-carotene
Mg/dl
1
30
1/21±16/50
2
30
1/14±13/63
Open days
Day
1
30
6±124
2
30
7±92
1. Cattle belong to summer season
2. Cattle belong to winter season
of other researches [12]. In this study concentration
of Vit A and β-carotene in serum of cattle under heat
stress was more than cattle in winter season, which
this subject can be for fresh fodder in summer and
most β-carotene of their [5].
8.
9.
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