Maximizing Water Use Efficiency by Roselle at South – East... El- Boraie, F.M. and A.M. Gaber
by user
Comments
Transcript
Maximizing Water Use Efficiency by Roselle at South – East... El- Boraie, F.M. and A.M. Gaber
Journal of Applied Scienes Research, 5(1): 21-32, 2009 © 2009, INSInet Publication Maximizing Water Use Efficiency by Roselle at South – East of Egypt El- Boraie, F.M. and A.M. Gaber Desert Research Center (DRC), Cairo, Egypt. Abstract: A substantial experiment was conducted throughout two consecutive growing seasons of 2005/2006 and 2006/2007 at the experimental station of Shalatien, South – East of Egypt to study the optimizing irrigation schedule for maximizing water use efficiency by Roselle (Hibiscus sabdariffa). This work was the accomplished by designing 75 experimental units with the factorial experiment, split plot design. The main plots were water quantities and the sub-main plots were plant densities. Crop coefficient, water use efficiency and water economy under drip irrigation system were calculated and irrigation schedules were estimated. The obtained results indicated that the maximum leaves, stems and total fresh and dry weight were obtained by increasing the irrigation water dose to 100 % of ETm (Q 3) and/ or by decreasing plant densities. The highest values of fresh and dry sepals and seeds were associated with Q 2D 1 (80% ETm + low plant density) treatment with insignificant difference of Q 3D 1 treatment. ETa at all growth stages increased as irrigation water depth increased and /or plant density decreased. Also, data showed that flowering stage period consumed highest water. W ater use efficiency (W UE), water economy (W Ec) of both Hibiscus sabdariffa sepals and seeds affected by irrigation water amounts and plant densities. Maximum W UE values were obtained when applying 80% of ETm (Q 2) and /or sowing 28000 plants/fed. (D 1 ). Concerning the interaction effect, data indicated that the highest values were obtained when applying Q 2 D 1 treatment for the two consecutive growing seasons, while the lowest values were associated with Q 5 D 3 treatment. The recommended Kc values are 0.35, 0.68, 1.06 and 0.53 for initial, vegetative, flowering and ripening stages, respectively. Kc values increased as irrigation water depth increased and/or plant density decreased. The highest Kc values were obtained for the flowering growth stage (0.66 to 1.50), while the lowest values were recorded at the initial stage (0.18 to 0.46). Values of polysaccharides tended to increase with increasing irrigation water quantities and plant densities. W hile soluble sugar increased by decreasing irrigation water quantities and increasing plant densities for the two successive seasons. Key words: Irrigation schedule, Kc, water use efficiency, Hibiscus sabdariffa, irrigation water quantities, plant densities, water economy, crop coefficient. acidulous refreshing hot or cold bavarages and infusions which are a popular admired drink in many hot climatic countries. It is known that the plant vegetative characteristics have a direct relation with agricultural practices such as plant densities, which mostly affected the vegetative growth and then the yield. The competition for light, water and minerals is reflected on metabolic processes in plant. In this respect,[1 4 ] . reported that plant height was increased by decreasing planting distances. Also, they found that the highest yield was obtained from planting on one side ridge and 50 cm spacing between plants. In addition, they showed that the percentage of anthocyanins content and total organic acid in dried sepals decreased significantly as the highest of plant densities.[2 1 ] found that the widest spacing produced the greatest number of fruits per plant. However, the narrower spacings INTRODUCTION In the course of land reclamation and improvement, Medicinal and Aromatic plants were recognized as being useful in desert regions with its climatic conditions which is favorable for the growth of these plants and the extension in these regions is promising. All reviews showed clearly that benefits were associated with structural improvements, Roselle (Hibiscus sabdariffa L.); Karkadeih, is an annual or perennial bushy sub shrub, about 5-8 feet height, with branched, erect, smooth and often purplish stem, belonging to Family Malvaceae. This plant is indigenous to tropic Africa[1 5 ] . It can be planted fairly in wide range of countries in tropical and subtropical regions. The main product of this crop is the dried fleshy epicalyx and calyx, which are used in preparing Corresponding Author: El- Boraie, F.M., Desert Research Center (DRC), Cairo, Egypt. 21 J. Appl. Sci. Res., 5(1): 21-32, 2009 gave greater fruit and fresh calyces weights and higher yields of dry calyces. The best results were obtained with the 50cm. spacing. The average calyces yield was 214.8 – 218.9 kg/fed for Masri and 187.1 – 190.4 kg/fed for Sudani. In addition, they mentioned that the high densities gave higher anthocyanins per unit area. But didn't significantly affect the calyx content of anthocyanins.[2 ] showed that the total carbohydrates content in herb of Pimpinella anisum increased by increasing the planting distances from 20 to 80 cm. In the present study, the combined effect of irrigation water depths (ETm) and plant densities on water consumptive use (ETa), crop coefficient (Kc), water use efficiency and Roselle (Hibiscus sabdariffa) yield were investigated. plants/fed. The experimental plots were bounded by 1 m wide bare soil to avoid horizontal water infiltration. Immediately before irrigation and after by 3 hours, the soil moisture content were gravimetrically determined on dry basis for three depths 0-20, 20-40 and 40-60 cm. Seeds were sown on the 15 th March in the two successive seasons. Nitrogen and potassium were applied through three equal doses using 75 kg /fed. of both N and K 2 O as ammonium and potassium sulphate. The first applied dose was done after 15 days of planting, while the 2 n d and the 3 rd ones were added after 45 and 90 days from cultivation. W hile phosphorus as superphosphate (62 kg P 2 O 5 /fed) was added 15 days before cultivation. Also, 10 ton/fed. of sheep dung as an organic manure was thoroughly mixed with 0-20 cm soil surface layer two weeks before planting.. The analysis of the used organic manure is shown in Table (4). The plants were harvested on 10 th of October. The actual consumptive use for each stage and for total season were calculated. The water use efficiency was calculated[1 2] . and also, water economy was estimated according to [2 3 ] . Crop coefficient calculated according to[24 ] . The plants from all treatments were taken to determine plant height (cm), fresh and dry weights of leaves and stems per plant (g), number of calyx per plant, fresh and dry weights of calyx and sepals (g/plant), total yield of dry sepals (kg/fed.), seed yield per plant and per feddan, determination of total carbohydrates percentage according to [ 1 7 ] . and determination of total anthocyanins, according to [8 ]. for Roselle. M ATERIALS AND M ETHODS A field experiment was conducted throughout two consecutive growing seasons of 2005/2006 and 2006/2007 at the experimental station of Shalatien, South-East of Egypt, Red Sea Governorate. The station is located at 22 o to 24 o N latitude, 35 o 30 \ to 36 o 30 \ E longitude. The physical and chemical of soil properties were analyzed according to [1 8 ]. Table (1a & b), chemical characteristics of applied irrigation water were determined according to [1 3 ,1 9] as shown in Table (2). Roselle seeds were obtained from Department of the Medicinal and Aromatic Plants, Agricultural Research Center, Giza, Egypt. Split plot design with four replicates was used to study the combined effect of applying irrigation water quantities and plant densities on yield, actual evapotranspiration (ETa), crop coefficient (Kc) and efficiency of water use by Roselle plants. Five irrigation water amounts (ETm) were obtained from the product of the potential evapotranspiration (ETo) by crop coefficient for every stage of Roselle then multiplying by 0.6, 0.8, 1.0, 1.2 and 1.4 (i.e., 60%, 80%, 100%, 120%, and 140%). The ETo were calculated from Penman-Monteith equation [5 ]. The anticipated crop coefficient was 0.35, 0.78, 1.2 and 0.6 for establishment, vegetative, flowering and ripening stages, respectively. Every two days, the irrigation water amounts were applied by drip irrigation system (50 cm between laterals and 30 cm between drippers) through 4 l/h emitters, caused 25 cm diameter of wetted zone, thus the fraction of the surface wetted, fw, may be only 0.33 [5 ] stated that for a trickle irrigation system, the fraction of the surface wetted, fw, may be only 0.33). The depths of irrigation doses for plant growth stages are shown in Table (3). Also, three plant densities, 1, 2 and 3 seedlings were let to grow under each emitter, i.e. 28000, 56000 and 84000 RESULTS AND DISCUSSION Concerning the plant height as affected by different plant density and irrigation water quantities treatments, data presented in Table (5) showed that the values increased with increasing irrigation water amount and/ or decreasing the plant density. The plant height increased significantly by applying 120 % of ETm irrigation water dose, reached 157.5 and 163.6 cm in the 1st & 2nd seasons, respectively. W hile these values increased significantly by applying the lightest densities (D 1 ), and reached 159.1 and 166.5 cm in the 1 st & 2 n d seasons, respectively. Concerning the interaction effect of irrigation water quantities and plant density, data showed that the tallest plants reached 175.1 and 175.7 cm by applying Q 2 D 1 treatment in the first and second seasons, respectively. This may be attributed to the competition between plants for obtaining more light. These results were agreed with those reported by [1 4 ,6 ] . on Roselle plants and [2 ] . on anise plants. 22 J. Appl. Sci. Res., 5(1): 21-32, 2009 Table 1a: Som e physical properties of the studied soil in Shalatien location. D epth, Particle size distribution % T.C Pd M oisture cm ---------------------------------------------------------------------------(g/cm 3 ) content% Coarse Sand M id.Sand Fine Sand Silt Clay 0-20 3 31 61 3 2 Sandy 1.66 4.85 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------20-40 6 22 66 4 2 Sandy 1.69 4.53 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------40-60 5 25 61 6 3 Sandy 1.68 4.42 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------60-80 5 29 57 6 3 Sandy 1.67 4.66 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------80-100 5 30 56 6 3 Sandy 1.66 3.81 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------100-120 5 31 55 6 3 Sandy 1.65 3.81 Table 1.b: Som e chem ical properties of the studied soil in Shalatien location. D epth EC pH CaCO 3 Cations m e/l Anions m e/l cm dS/m % --------------------------------------------------------------------------------------------------N a+ K+ Ca + + M g+ + CO 3 = H CO 3 ClSO 4 -0-20 0.77 7.7 2.3 0.62 0.49 3.66 2.91 0.42 3.92 3.33 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------20-40 0.66 7.6 1.10 0.63 0.40 2.82 2.75 0.42 3.09 3.09 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------40-60 0.49 7.5 0.85 0.55 0.39 1.99 1.96 0.36 2.8 1.90 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------60-80 0.50 7.5 0.60 0.51 0.42 2.81 1.26 0.38 2.65 1.95 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------80-100 0.36 7.3 0.58 0.48 0.38 1.92 0.80 0.26 1.75 1.61 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------100-120 0.34 7.3 0.55 0.32 0.38 1.19 1.51 0.39 1.52 1.49 Table 2: Irrigation water chem ical analysis. ECdSm -1 PH Cations m e/l -----------------------------------------------Ca + + M g+ + N a+ K+ 1.2 7.6 0.49 0.30 0.30 0.11 Anions m e/l --------------------------------------------------CO 3 = H CO 3 ClSO 4 0.12 0.68 0.38 SAR = 0.78 Table 3: N et applied irrigation water treatm ents (m m & m 3 /fed.) at different growth stage periods (days). Irrigation water Growth stages Total applied quantities -------------------------------------------------------------------------------------------------------------------------water (195 days) Initial (30 days) Vegetative((50 days) Flowering (60 days) Y ield form . (55 days) --------------------------------------------------------------------------------------------------3 3 3 mm m / fed. mm m / fed. mm m / fed. mm m 3 / fed. mm m 3 / fed. Q 1 (60% ) 40.79 56.54 183.79 254.73 396.47 549.52 211.47 293.10 832.53 1153.89 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 2 (80% ) 54.39 75.39 245.05 339.64 528.63 732.69 281.96 390.80 1110.04 1538.52 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 3 (100% ) 67.99 94.24 306.31 424.55 660.79 915.86 352.45 488.50 1387.55 1923.15 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 4 (120% ) 81.59 113.09 367.57 509.46 792.95 1099.03 422.94 586.20 1665.06 2307.78 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 5 (140% ) 95.19 131.94 428.83 594.37 925.11 1282.20 493.43 683.90 1942.57 2692.41 Table 4: The properties of the applied organic m anure. O rganic m anure O rganic carbon % Sheep dung 23.71 Total nitrogen % 2.06 Data showed that 100 % ETm irrigation water amount (Q 3 ) gave the highest number of branches/ plant, which reached 8.1 & 9.7 at the two seasons respectively. Also, these values increased significantly by increasing the plant density and reached its maximum value (9.0 & 11.5 at the studied two seasons, respectively) by applying the heaviest plant density. Concerning the interaction effect of irrigation water quantities and plant density, data showed that the highest number of branches per plant in the two seasons was associated with applying Q 2D 3 treatment O rganic m atter % 40.76 C/N ratio 11.51 which reached 9.5 and 12.7, while the lowest one was associated with the Q 1 D 1 treatment. Such values reached 5.0 and 5.9 branches per plant for the two seasons, respectively. These results were in harmony with those reported by[7 ] . on celery and [2 ] . on anise plants. Also, data showed a positive significant correlation between the number of branches/plant and the plant height (0.84 ** & 0.72 ** at the two seasons, respectively). 23 J. Appl. Sci. Res., 5(1): 21-32, 2009 Table 5: Effect of irrigation water quantities and plant densities on plant height and num ber of branches per plant of Roselle. Irrigationwaterquantities D Plant height (cm ) -----------------------------------------1st season 2n d season N um ber of branches/plant ------------------------------------------1st season 2nd season Q 1 60% D1 140.2 145.6 5.0 5.9 --------------------------------------------------------------------------------------------------------------------------------------D2 135.0 140.8 6.5 7.0 ---------------------------------------------------------------------------------------------------------------------------------------D3 130.2 135.6 7.3 10.5 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 2 80% D1 175.1 175.7 6.3 7.0 ----------------------------------------------------------------------------------------------------------------------------------------D2 152.3 160.0 8.0 9.0 ---------------------------------------------------------------------------------------------------------------------------------------D3 150.0 144.1 9.5 12.7 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 3 100% D1 163.8 178.3 6.7 7.3 ----------------------------------------------------------------------------------------------------------------------------------------D2 152.3 154.7 8.3 9.3 ----------------------------------------------------------------------------------------------------------------------------------------D3 146.8 149.0 9.2 12.5 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 4 120% D1 166.2 170.2 7.0 7.3 ----------------------------------------------------------------------------------------------------------------------------------------D2 155.4 161.3 8.5 9.0 ---------------------------------------------------------------------------------------------------------------------------------------D3 150.8 159.2 9.3 11.2 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 5 140% D1 150.2 162.5 6.1 6.5 ---------------------------------------------------------------------------------------------------------------------------------------D2 140.3 151.6 7.2 8.2 ---------------------------------------------------------------------------------------------------------------------------------------D3 138.1 143.2 9.5 10.7 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LSD 5 % Q 0.50 1.49 0.86 0.73 ----------------------------------------------------------------------------------------------------------------------------------------D 0.68 0.51 0.61 0.67 ---------------------------------------------------------------------------------------------------------------------------------------Q X D 1.52 1.13 1.37 1.50 Results in Table (6) indicated that the number of fruits per plant, fresh and dry weights of fruits per plant increased significantly by increasing the irrigation water dose to 100 % of ETm and by decreasing plant densities. Respecting to the interaction effect of irrigation water quantities and plant density, data showed that highest values of number of fruits per plant fresh and dry weights of fruits per plant were recorded by D 1 Q 3 treatment. Such increases reached 80.9 & 186.1, 105.4 & 130.2 and 162.2 & 131.1 % relative to D 3 Q 1 treatment at the two seasons, respectively. These results were in agreement with those by[4 ,2 1 ] . on Roselle plants, and also in harmony with those obtained by[2 ] . on anise plants, who found that the lowest fruit yield per plant was obtained when plants more dense. Also, data showed a positive significant correlation between the plant height and both number of fruits per plant, fresh and dry weights of fruits per plant, also between number of fruits per plant and both fresh and dry weights of fruits per plant, while it is noticed that the same parameters decreased as the number of branches / plant increased (negative significant correlation). Data in Table (7) showed that the maximum leaves, stems and total fresh and dry weight were obtained by increasing the irrigation water dose to 100 % of ETm and/ or by decreasing plant densities. Also, all weights were recorded highly significant increases by applying Q 2 D 1 treatment with unsignificant at applying Q 3 D 1 treatment. For example, the highest values of total dry weight were recorded as 6.0 and 7.1 (ton/fed.) in the two studied seasons, respectively. Such increases reached 180 and 173 % relative to the lowest values which associated with Q 1 D 3 treatment. Similar results were obtained by[2 ] . on anise and [16 ] . on senna plants, who reported that decreasing densities resulted in the continuous increase in fresh and dry weights of plant. 24 J. Appl. Sci. Res., 5(1): 21-32, 2009 Table 6: Effect of irrigation water quantities and plant densities on num ber, fresh and dry weight of fruits per plant. Irrigation water D Num ber of fruits Fresh weight D ry weight of quantities per plant of fruits (g/plant) fruits (g/plant) --------------------------------------------------------------------------------------------------------1st season 2 n d season 1 st season 2 n d season 1 st season 2 n d season Q 1 60% --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D1 77.3 126.5 377.6 385.2 90.6 110.4 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D2 75.3 66.4 290.2 295.8 65.2 75.1 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D3 53.4 50.4 203.5 216.9 52.1 67.2 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 2 80% --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D1 90.6 140.6 409.8 480.5 130.9 150.6 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D2 90.1 91.3 322.4 330.3 90.2 130.7 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D3 65.7 71.1 233.7 265.0 73.0 104.0 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 3 100% --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D1 96.6 144.2 418 499.2 136.6 155.3 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D2 92.1 93.3 329.5 339.0 93.2 131.9 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D3 63.4 66.8 232.8 250.2 71.9 99.6 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 4 120% --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D1 90.6 120.8 402.5 410.6 99.7 110.6 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D2 90.1 95.5 305.2 315.1 70.8 80.1 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D3 61.8 71.9 230.2 240.3 60.1 65.1 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 5 140% --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D1 80.7 100.7 380.2 395.6 81.6 93.7 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D2 80.3 92.8 270.3 300.9 60.2 70.9 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D3 55.2 66.9 193.6 221.2 48.2 52.2 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LSD --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 0.22 0.33 0.47 0.94 0.16 0.42 5% --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D 0.21 0.12 0.28 0.46 0.40 0.25 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q XD 0.48 0.26 0.63 1.02 0.90 0.57 Data in Table (8) showed that the highest values of fresh and dry sepals and seeds were associated with Q 2 D 1 treatment with insignificant difference of Q 3D 1 treatment, while the lowest values were associated with Q 5 D 3 treatment. Such increases reached 42.8, 58.8 % and 98.2, 100.8 % for fresh and dry weights, also reached 69.1 and 164.6 % for seeds weight in the two studied seasons, respectively. These results agreed with those reported by[2 2,9 ,1 4 ] . on Roselle plants and [2 ] . on anise plants. These increases as a result of decreasing the plant densities might be due to the competition between plants for obtaining more light and nutrients. Concerning the effect of irrigation water amounts and plant densities on Roselle actual evapotranspiration (ETa), data presented in Tables (9 & 10) showed that ETa at all growth stages increased as irrigation water depth increased and /or plant density decreased. The maximum ETa values were obtained under applying the largest irrigation water dose (Q 5) and lowest plant density (D 1 ) treatment, while the minimum values were associated with applying the lowest irrigation water amount (Q 1 ) and the highest plant density (D 3) treatment at seasonal and all growth stages for the two seasons. Also, data showed that flowering stage period consumed highest water. These results are in harmony with that obtained by [1 1 ,1 0 ]. 25 J. Appl. Sci. Res., 5(1): 21-32, 2009 Table 7: Effect of irrigation water quantities and plant densities on fresh and dry leaves, stem s and total weight (ton/fed.) Irrigation W ater Plant Fresh weight D ry weight quantities density ----------------------------------------------------------------------------------------------------------------------------------Leaves Stem s Total weight Leaves Stem s Total weight -----------------------------------------------------------------------------------------------------------------1s 2nd 1 st 1 st 2nd 1 st 1 st 2nd 1 st 2n d 1 st 2n d season season season season season season season season season season season season Q 1 60% D1 6.4 6.9 16.8 18.4 23.2 25.3 0.6 0.7 4.6 5.6 5.2 6.3 ---------------------------------------------------------------------------------------------------------------------------------------------------------D2 3.5 3.6 11.1 11.2 14.6 14.7 0.4 0.4 3.2 3.0 3.6 3.5 ---------------------------------------------------------------------------------------------------------------------------------------------------------D3 2.5 2.6 7.4 7.4 10.0 10.0 0.3 0.4 2.3 2.2 2.7 2.6 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 2 80% D1 7.0 7.1 17.5 21.6 24.5 28.7 1.0 1.1 5.0 6.0 6.0 7.1 ---------------------------------------------------------------------------------------------------------------------------------------------------------D2 3.7 3.7 11.5 11.9 15.2 15.6 0.6 0.6 3.4 3.4 4.0 4.1 --------------------------------------------------------------------------------------------------------------------------------------------------------D3 2.6 2.8 8.4 9.0 11.0 11.7 0.5 0.6 2.5 2.7 3.0 3.2 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 3 100% D1 7.1 7.2 17.6 21.9 24.7 29.1 1.1 1.2 5.2 6.3 6.3 7.4 ---------------------------------------------------------------------------------------------------------------------------------------------------------D2 3.7 3.7 11.9 12.0 15.6 15.7 0.7 0.7 3.4 3.5 4.1 4.2 ---------------------------------------------------------------------------------------------------------------------------------------------------------D3 2.6 2.7 8.1 8.9 10.7 11.5 0.5 0.5 2.5 2.6 2.9 3.1 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 4 120% D1 7.0 7.2 17.5 21.4 24.6 28.6 1.0 1.1 5.0 6.2 6.0 7.3 ---------------------------------------------------------------------------------------------------------------------------------------------------------D2 3.7 3.7 11.7 11.8 15.4 15.5 0.6 0.7 3.4 3.9 4.0 4.6 ---------------------------------------------------------------------------------------------------------------------------------------------------------D3 2.6 2.6 8.3 8.4 10.9 11.1 0.5 0.5 2.5 2.6 2.9 3.1 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 5 140% D1 6.8 7.0 17.0 21.1 23.9 28.0 0.8 1.0 4.8 5.8 5.7 6.8 ---------------------------------------------------------------------------------------------------------------------------------------------------------D2 3.5 3.6 11.5 11.6 15.0 15.2 0.5 0.6 3.3 3.5 3.8 4.1 ---------------------------------------------------------------------------------------------------------------------------------------------------------D3 2.5 2.6 8.1 8.3 10.7 10.9 0.4 0.5 2.3 2.4 2.7 2.9 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LSD 5 % Q 0.20 0.16 0.16 0.50 1.30 0.38 0.20 0.34 0.34 0.16 0.42 0.40 ----------------------------------------------------------------------------------------------------------------------------------------------------------D 0.26 0.22 0.18 0.22 0.91 0.28 0.16 0.28 0.24 0.18 0.34 0.44 --------------------------------------------------------------------------------------------------------------------------------------------------------Q XD 0.58 0.50 0.42 0.50 2.03 0.64 0.34 0.62 0.56 0.42 0.76 1.00 Table 8: Effect of irrigation water quantities and plant densities on fresh & dry sepals yield and seeds of Roselle (kg/fed.). IrrigationW ater Plant Sepals yield Seeds yield quantities density -------------------------------------------------------------------------------------Fresh weight D ry weight ------------------------------------------------------------------------1st season 2 n d season 1 st season 2 n d season 1 st season 2 n d season Q 1 60% D1 2164.0 2652.0 368.0 380.0 370.0 404.0 ------------------------------------------------------------------------------------------------------------------------------------------------------D2 2043.5 2042.5 242.5 266.5 406.0 432.0 -----------------------------------------------------------------------------------------------------------------------------------------------------D3 1751.0 1801.0 223.0 235.0 272.7 286.7 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 2 80% D1 2220.0 2900.0 440.0 502.0 462.0 762.0 ------------------------------------------------------------------------------------------------------------------------------------------------------D2 2127.5 2201.0 384.0 410.5 443.0 482.0 -----------------------------------------------------------------------------------------------------------------------------------------------------D3 1804.0 2105.0 334.0 401.0 310.0 457.3 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 3 100% D1 2234.0 2920.0 444.0 510.0 468.0 780.0 -----------------------------------------------------------------------------------------------------------------------------------------------------D2 2131.0 2211.5 385.0 416.5 451.0 490.0 -----------------------------------------------------------------------------------------------------------------------------------------------------D3 1801.0 2100.0 330.0 386.0 305.3 435.3 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 26 J. Appl. Sci. Res., 5(1): 21-32, 2009 Table 8:C ontinue Q 4 120% D1 2204.0 2800.0 400.0 480.0 464.0 804.0 ------------------------------------------------------------------------------------------------------------------------------------------------------D2 2105.0 2126.5 360.0 366.0 450.0 500.0 -----------------------------------------------------------------------------------------------------------------------------------------------------D3 1776.0 2001.0 303.0 302.0 310.0 466.7 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 5 140% D1 2008.0 2762.0 346.0 370.0 400.0 460.0 ------------------------------------------------------------------------------------------------------------------------------------------------------D2 1807.0 2045.0 229.0 234.0 400.0 426.0 -----------------------------------------------------------------------------------------------------------------------------------------------------D3 1555.0 1826.0 222.0 250.0 273.3 288.0 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LSD 5 % Q 11.66 13.44 16.06 17.12 4.95 4.86 -----------------------------------------------------------------------------------------------------------------------------------------------------D 12.45 10.29 10.94 11.60 3.93 3.99 -----------------------------------------------------------------------------------------------------------------------------------------------------Q XD 13.25 23.02 24.45 25.95 2.08 2.22 Values of crop coefficient (Kc) of Hibiscus sabdariffa for different growth stages under all studied treatments were calculated according [2 4 ] . and presented in Table (12). T he results showed that the recommended Kc values under Q 3 (100 % ETm) + D1 (low plant density) treatment are 0.35, 0.68, 1.06 and 0.53 for initial, vegetative, flowering and ripening stages, respectively. It is clearly found that Kc values increased as irrigation water depth increased and/or plant density decreased. The highest Kc values were obtained for the flowering growth stage (0.66 to 1.50), while the lowest values were recorded at the initial stage (0.18 to 0.46). Data in Table (13) stated that the mean values of poly saccharides (mg/g) tended to increase with increasing irrigation water quantities and plant densities as well. W hile soluble sugar (mg/g) increased by decreasing irrigation water quantities and increasing plant densities for the two successive seasons. Data presented in Table (11) showed the water use efficiency (W UE) and water economy (W Ec) of both Hibiscus sabdariffa sepals and seeds affected by irrigation water amounts and plant densities. Maximum W UE values were obtained when applying 80% of ETm (Q 2 ) and /or sowing 28000 plants/fed. (D 1). Concerning the interaction effect, data indicated that the highest values were obtained when applying Q 2 D 1 treatment for the two consecutive growing seasons, while the lowest values were associated with Q 5D 3 treatment. For example, water use efficiency by seeds at 2n d season increased by 144 % at applying Q 2D 1 treatment relative to its value at applying Q 1D 1 and by 400 % relative to its value at applying Q 5D 3 . Such increases for water economy reached 143 and 455 % for the previous mentions coparesons, respectively. The present results are in harmony with that obtained by[1 1 ,2 0 ] . on peas, beans and fodder beet,[3 ,1 ] . on sugar beet and [1 0 ] . Table 9: Actual evapotranspiration (ETa), (m m & m 3 /fed.) at different growth stages and total season (fw = 0.33) by Roselle at the 1 st season. Irrigation wate Plant Growth stages Total Eta (195 days) rquantities density ------------------------------------------------------------------------------------------------------------Initial Vegetative Flowering Ripening -------------------------------------------------------------------------------------mm m 3 / fed. mm m 3 / fed. mm m 3 / fed. mm m 3 / fed. mm m 3 / fed. Q 1 60% D1 36.67 50.82 180.6 250.31 388.54 538.52 200.01 277.21 805.82 1116.86 ------------------------------------------------------------------------------------------------------------------------------------------------------------D2 35.01 48.52 178.52 247.43 379.22 525.60 192.89 267.35 785.64 1088.90 -----------------------------------------------------------------------------------------------------------------------------------------------------------D3 33.10 45.88 166.3 230.53 362.11 501.88 184.23 255.34 745.77 1033.64 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 2 80% D1 50.53 70.03 229.2 317.71 518.06 718.03 242.49 336.09 1040.30 1441.86 ------------------------------------------------------------------------------------------------------------------------------------------------------------D2 49.76 68.96 220.64 305.81 507.12 702.87 230.01 318.79 1007.53 1396.44 -----------------------------------------------------------------------------------------------------------------------------------------------------------D3 47.59 65.95 213.1 295.40 485.98 673.57 223.77 310.15 970.47 1345.07 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 3 100% D1 64.64 89.58 262.8 364.24 574.89 796.80 303.11 420.11 1205.43 1670.73 -----------------------------------------------------------------------------------------------------------------------------------------------------------D2 63.55 88.08 253.8 351.87 503.25 697.50 285.55 395.77 1106.23 1533.23 -----------------------------------------------------------------------------------------------------------------------------------------------------------D3 60.76 84.21 245.8 340.70 489.68 678.70 268.47 372.10 1064.72 1475.70 27 J. Appl. Sci. Res., 5(1): 21-32, 2009 Table 9: C ontinue Q 4 120% ------------------------------------------------------------------------------------------------------------------------------------------------------------D1 77.04 106.77 283.8 393.43 689.87 956.16 363.73 504.13 1414.49 1960.49 -----------------------------------------------------------------------------------------------------------------------------------------------------------D2 74.87 103.76 271.96 376.94 678.88 940.93 347.25 481.29 1372.96 1902.92 ------------------------------------------------------------------------------------------------------------------------------------------------------------D3 72.23 100.11 263.80 365.62 659.23 913.69 333.75 462.58 1329.01 1842.00 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 5 140% ------------------------------------------------------------------------------------------------------------------------------------------------------------D1 84.59 117.24 304.35 421.83 814.74 1129.23 424.35 588.15 1628.03 2256.45 ------------------------------------------------------------------------------------------------------------------------------------------------------------D2 82.11 113.80 282.00 390.85 800.22 1109.10 405.59 562.15 1569.92 2175.91 -----------------------------------------------------------------------------------------------------------------------------------------------------------D3 79.66 110.40 273.05 378.45 792.01 1097.73 387.65 537.28 1532.37 2123.86 Table 10: Actual evapotranspiration )ETa(, )m m & m3 /fed.( at different growth stage and total season )fw = 0.33( by Roselle the 2 n d season. Irrigation wate Plant Growth stages Total Eta (195 days) rquantities density ------------------------------------------------------------------------------------------------------------Initial Vegetative Flowering Ripening -------------------------------------------------------------------------------------mm m 3 / fed. mm m 3 / fed. mm m 3 / fed. mm m 3 / fed. mm m 3 / fed. Q 1 60% D1 38.86 53.87 182.41 252.81 394.37 546.59 205.01 284.14 820.65 1137.42 -------------------------------------------------------------------------------------------------------------------------------------------------------------D2 37.15 51.49 180.31 249.90 384.91 533.48 197.71 274.03 800.08 1108.90 ------------------------------------------------------------------------------------------------------------------------------------------------------------D3 36.06 49.98 167.99 232.84 367.54 509.41 188.84 261.73 760.43 1053.96 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 2 80% D1 53.56 74.24 231.52 320.89 525.83 728.80 248.55 344.49 1059.46 1468.41 ------------------------------------------------------------------------------------------------------------------------------------------------------------D2 52.33 72.53 222.85 308.87 514.73 713.41 235.76 326.76 1025.67 1421.58 -----------------------------------------------------------------------------------------------------------------------------------------------------------D3 50.44 69.91 215.27 298.36 493.27 683.67 229.36 317.90 988.34 1369.84 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 3 100% D1 67.98 94.22 265.43 367.88 583.51 808.75 310.68 430.61 1227.60 1701.46 ------------------------------------------------------------------------------------------------------------------------------------------------------------D2 67.36 93.37 256.42 355.39 510.80 707.97 292.69 405.67 1127.27 1562.39 ------------------------------------------------------------------------------------------------------------------------------------------------------------D3 65.41 90.66 248.27 344.10 497.03 688.88 275.18 381.40 1085.89 1505.04 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 4 120% D1 81.66 113.18 286.70 397.37 700.22 970.50 372.82 516.73 1441.40 1997.77 ------------------------------------------------------------------------------------------------------------------------------------------------------------D2 80.36 111.38 274.68 380.71 689.06 955.04 355.93 493.32 1400.04 1940.45 ------------------------------------------------------------------------------------------------------------------------------------------------------------D3 77.56 107.50 266.44 369.28 669.12 927.40 342.09 474.14 1355.21 1878.32 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 5 140% D1 89.67 124.28 307.40 426.05 826.96 1146.17 434.96 602.85 1658.98 2299.35 ------------------------------------------------------------------------------------------------------------------------------------------------------------D2 88.11 122.12 284.82 394.76 812.22 1125.74 415.73 576.20 1600.88 2218.82 ------------------------------------------------------------------------------------------------------------------------------------------------------------D3 85.23 118.13 275.78 382.23 803.89 1114.19 397.34 550.71 1562.24 2165.27 Table 11: W ater use efficiency, W ater econom y as affected by irrigation water quantities and plant densities of Roselle. Irrigation W ater Plant W U E (kg/m 3 ) W ater Econom y (kg/m 3 ) quantities density ------------------------------------------------------------------------------------------------------------------------Sepals Seeds Sepals Seeds ----------------------------------------------------------------------------------------------------------st nd st nd st nd 1 season 2 season 1 season 2 season 1 season 2 season 1 st season 2n d season Q 1 60% D1 0.32 0.33 0.33 0.36 0.32 0.33 0.32 0.35 -----------------------------------------------------------------------------------------------------------------------------------------------------D2 0.22 0.24 0.37 0.39 0.21 0.23 0.35 0.37 -----------------------------------------------------------------------------------------------------------------------------------------------------D3 0.22 0.22 0.26 0.27 0.19 0.20 0.24 0.25 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 2 80% D1 0.31 0.34 0.32 0.52 0.29 0.33 0.30 0.50 -----------------------------------------------------------------------------------------------------------------------------------------------------D2 0.27 0.29 0.32 0.34 0.25 0.27 0.29 0.31 ----------------------------------------------------------------------------------------------------------------------------------------------------D3 0.25 0.29 0.23 0.33 0.22 0.26 0.20 0.30 28 J. Appl. Sci. Res., 5(1): 21-32, 2009 Table 11: C ontinue Q 3 100% D1 0.27 0.30 0.28 0.46 0.23 0.27 0.24 0.41 -----------------------------------------------------------------------------------------------------------------------------------------------------D2 0.25 0.27 0.29 0.31 0.20 0.22 0.23 0.25 -----------------------------------------------------------------------------------------------------------------------------------------------------D3 0.22 0.26 0.21 0.29 0.17 0.20 0.16 0.23 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 4 120% D1 0.20 0.24 0.24 0.40 0.17 0.21 0.20 0.35 -----------------------------------------------------------------------------------------------------------------------------------------------------D2 0.19 0.19 0.24 0.26 0.16 0.16 0.19 0.22 ----------------------------------------------------------------------------------------------------------------------------------------------------D3 0.16 0.16 0.17 0.25 0.13 0.13 0.13 0.20 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 5 140% D1 0.15 0.16 0.18 0.20 0.13 0.14 0.15 0.17 -----------------------------------------------------------------------------------------------------------------------------------------------------D2 0.11 0.11 0.18 0.19 0.09 0.09 0.15 0.16 ----------------------------------------------------------------------------------------------------------------------------------------------------D3 0.10 0.12 0.13 0.13 0.08 0.09 0.10 0.11 Table 12: Growth stages and seasonal crop coefficient (Kc) of Roselle at the two seasons. Irrigation W ater Plant Crop coefficient (Kc) quantities density ------------------------------------------------------------------------------------------------------------------------------------------Growth stages Seasonal --------------------------------------------------------------------------------------------------------------Initial Vegetative Flowering Ripening ------------------------------------------------------------------------------------------1 st 2nd 1 st 2nd 1 st 2nd 1 st 2nd 1 st 2nd season season season season season season season season season season Q 1 60% D1 0.19 0.20 0.46 0.46 0.71 0.72 0.34 0.35 0.47 0.48 -----------------------------------------------------------------------------------------------------------------------------------------------------------D2 0.18 0.19 0.45 0.46 0.69 0.70 0.33 0.34 0.46 0.46 -----------------------------------------------------------------------------------------------------------------------------------------------------------D3 0.17 0.19 0.42 0.43 0.66 0.67 0.31 0.32 0.43 0.44 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 2 80% D1 0.26 0.28 0.58 0.59 0.94 0.95 0.41 0.42 0.60 0.61 -----------------------------------------------------------------------------------------------------------------------------------------------------------D2 0.26 0.27 0.56 0.57 0.92 0.93 0.39 0.40 0.58 0.59 -----------------------------------------------------------------------------------------------------------------------------------------------------------D3 0.24 0.26 0.54 0.55 0.88 0.90 0.38 0.39 0.56 0.57 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 3 100% D1 0.33 0.35 0.67 0.68 1.04 1.06 0.52 0.53 0.70 0.71 -----------------------------------------------------------------------------------------------------------------------------------------------------------D2 0.33 0.35 0.65 0.65 0.91 0.93 0.49 0.50 0.64 0.65 -----------------------------------------------------------------------------------------------------------------------------------------------------------D3 0.31 0.34 0.63 0.63 0.89 0.90 0.46 0.47 0.62 0.63 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 4 120% D1 0.40 0.42 0.72 0.73 1.25 1.27 0.62 0.63 0.82 0.84 -----------------------------------------------------------------------------------------------------------------------------------------------------------D2 0.39 0.41 0.69 0.70 1.23 1.25 0.59 0.61 0.80 0.81 ----------------------------------------------------------------------------------------------------------------------------------------------------------D3 0.37 0.40 0.67 0.68 1.20 1.22 0.57 0.58 0.77 0.79 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q 5 140% D1 0.44 0.46 0.78 0.78 1.48 1.50 0.72 0.74 0.94 0.96 -----------------------------------------------------------------------------------------------------------------------------------------------------------D2 0.42 0.45 0.72 0.73 1.45 1.47 0.69 0.71 0.91 0.93 -----------------------------------------------------------------------------------------------------------------------------------------------------------D3 0.41 0.44 0.70 0.70 1.44 1.46 0.66 0.68 0.89 0.91 The highest values of polysaccharides were associated with Q 5 D 3 treatment, i.e. 630 and 642 mg/g, while the lowest values were associated with Q 1 D 1 , i.e., 292.50 and 295.50 mg/g at the two seasons, respectively. The differences reached 115.6 and 117.3 %, for the two successive seasons, respectively. On the other hand, soluble sugar gave the highest value when treated by Q 1 D 3 treatment, i.e., 98.40 and 98.60 mg/g, while the lowest value was associated with Q 5D 1 treatment, i.e., 41.70 and 42.80 mg/g for the two successive seasons, respectively. Such increase reached 135.97 and 130.37%. The previous results may be due to the fact that the low density of the grown plants allowed more absorption of elements from the soil to plants also minimized the competition of plants for light and 29 J. Appl. Sci. Res., 5(1): 21-32, 2009 while the lowest percentages were associated with Q 1D 3 treatment (19.55 and 19.81 %). Such differences were nearly 75 and 24%. Also, data indicated that total acidity (%) increased by decreasing plant density and the lowest percentage was recorded at Q 3D 1 treatment (11.05 and 11.10 % at the two seasons, respectively). The highest anthocyanins (mg/plant) were formed in sepals of low density Roselle plants at both seasons. So, the highest values were recorded at Q 5D 1 treatment (99.12 and 109.90 mg/plant, at two seasons, respectively), while the lowest values were obtained under Q 1 D 3 (85.18 and 96.12 mg/g at two seasons respectively). water, thus increased the photosynthesis process which in turn stimulated the biosynthesis of sugars. Similar results coincided with those obtained by [4 ,2 1 ,1 4 ] . on Roselle plants. Results presented in Table (14) showed the effect of irrigation water quantities and plant densities on chemical properties of the sepals of Roselle plants. Data detected that total soluble solids percentages in dried sepals were increased with decreasing plant density and increasing the applied water depth. The highest percentages of total soluble solids were 34.21 and 34.52 % for the two successive seasons, respectively when low plant density irrigated with Q 4, Table 13: arbohydrates content (m g/g) in Roselle sepals as affected by plant densities and irrigation water quantities. Irrigation water quantities Poly Saccharides content Soluble Sugar content ----------------------------------------------------------------------------------------------------------------------------Plant densities M ean Plant densities M ean ---------------------------------------------------------------------------------D1 D2 D3 D1 D2 D3 -----------------------------------------------------------------------------------------------------------------------------------------1 st Season Q1 293 332 490 372 63 80 98 80 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q2 333 371 545 417 60 75 94 77 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q3 373 410 600 461 58 71 90 73 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q4 375 445 615 478 50 61 81 64 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q5 378 480 630 496 42 51 72 55 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M ean 350 408 576 445 55 68 87 70 2 n d Season Q1 296 341 496 377 63 82 99 81 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q2 341 380 552 424 62 77 94 78 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q3 381 431 616 476 60 72 91 74 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q4 386 456 638 493 53 62 82 66 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Q5 388 493 642 507 43 52 73 56 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M ean 358 420 589 455 56 69 88 71 Table 14: Effect of irrigation water quantities and Plant densities on chem ical properties of the sepals of Roselle plants. Irrigation W ater quantities Plant densities Total Soluble Solids % Total Acidity % Anthocyanins m g/plant Phlafonines m g/plant --------------------------------------------------------------------------------------------------------------------------1 st 2nd 1 st 2nd 1 st 2nd 1 st 2nd season season season season season season season season Q1 D1 22.61 22.67 11.10 11.12 95.18 106.00 6.57 6.62 ---------------------------------------------------------------------------------------------------------------------------------------------D2 21.04 21.34 12.10 12.12 86.04 106.0 6.57 6.62 ---------------------------------------------------------------------------------------------------------------------------------------------D3 19.55 19.81 13.10 13.12 85.18 96.12 6.57 6.62 Q2 D1 22.71 22.79 11.19 11.20 96.27 108.10 7.24 7.25 ---------------------------------------------------------------------------------------------------------------------------------------------D2 21.14 21.45 12.19 12.20 90.04 98.22 7.24 7.25 ---------------------------------------------------------------------------------------------------------------------------------------------D3 20.65 20.91 13.19 13.20 86.08 94.11 7.24 7.25 30 J. Appl. Sci. Res., 5(1): 21-32, 2009 Table 14: C ontinue Q3 Q4 Q5 D1 33.81 34.62 11.05 11.10 97.58 109.05 5.60 5.66 ---------------------------------------------------------------------------------------------------------------------------------------------D2 32.25 32.65 12.05 12.10 91.12 99.01 5.60 5.66 ---------------------------------------------------------------------------------------------------------------------------------------------D3 31.74 31.78 13.05 13.10 88.19 95.40 5.60 5.66 D1 34.21 34.52 11.07 11.12 98.52 109.85 5.69 5.74 ---------------------------------------------------------------------------------------------------------------------------------------------D2 33.45 33.85 12.07 12.12 92.15 99.55 5.69 5.74 ---------------------------------------------------------------------------------------------------------------------------------------------D3 32.24 32.97 13.07 13.12 89.20 96.18 5.69 5.74 D1 34.20 34.51 11.07 11.12 99.12 109.90 5.74 5.78 ---------------------------------------------------------------------------------------------------------------------------------------------D2 33.48 33.85 12.07 12.12 93.24 100.00 5.74 5.78 ---------------------------------------------------------------------------------------------------------------------------------------------D3 32.81 33.07 13.07 13.12 90.18 97.18 5.74 5.78 W ith respect to phlafonines, data revealed that applying irrigation water by Q 2 (80% ETm) produced the highest phlafonines content (7.24 and 7.25 mg/plant, at two seasons, respectively). Also, data showed insignificant effect of water amount on phlafonines content. 10. El-Boraie, F.M., 2006. Response of some canola cultivars to modern irrigation systems under Shalatien conditions. Meteorological Research Bulletin, 21: 41-57. 11. Gaber, A.M. and M.H. El-Dosouky, 1993. W ater requirements, water economy and yield of beans and drip irrigation. Zagazig J. Agric. Res., 20(4): 1373-1383. 12. Giriappa, S., 1983. W ater use efficiency in agriculture. Agricultural Development and Rural Transformation Unit. Institute for Social and Economic Change Bangalore. Oxford & IBH Publishing Co. 13. Jackson, M .L., 1967. Soil chemical analysis, Prentice Hall of India. New Delhi, India. 14. Khater, M.R. and S.K Ahmed, 1992. Effect of sowing dates and planting distances on vegetative growth, yield and active substances on Roselle plant. Agric. Res. Cent. Hort. Inst. Medicinal and Aromatic plant Section, Dokki. 15. Kirby, R.H., 1963. Vegetable Fibers, By Prof. Nicholes Pullunin, pp: 129-131. Inter– Science Publish Inc. New York. 16. M ansour, C.P., V.S. Kubsad and D.S. Gowda, 1995. Nutrient uptake in mesta (Hibiscus s a b d a r iffa , L .) u n d e r v a r yin g f e r t i l i z e r combinations. Univ. of Agric. Sci.. Dharwad, Karnataka, Indian. Madras Agricultural J., 82(6-8): 480-482. 17. Michl – Dubois, K.A., K. Gilles, J.K. Hamilton, P.A. Rebars and F. Smith, 1956. Colorimetric Method for Determination of Sugars and Related Substances. Anal., Chem., 28 -370. 18. Piper, C.S., 1950. Soil and plant analysis. 1st Ed., Inter Publishers, New York, USA., pp: 47-49. 19. Richards, L.A., 1954. Diagnosis and Improvement of saline and Alkali Soils U.S. salinity lab. Salt, W ashington, D.C., U.S.A., Agric. Handbook No.60. 20. Seidhom, S.H., 1995. A study on the water consumptive use of some crops grown under desert conditions. M.Sc. Thesis, Fac. Agric., Zagazig Univ., Egypt. REFERENCES 1. 2. 3. 4. 5. 6. 7. 8. 9. Abd El– Rahman, M.I., 2005. Soil and plant water potential as related to water use efficiency in some newly reclaimed lands. Ph.D. thesis Fac. Agric. Al – Azhar Univ., Egypt. Abd El Salam, E.Z., 1994. Effect of chemical fertilization and planting distances on growth and chemical composition of Pimpinella anisum palm. M. Sc. Thesis. Fac. Agric. Cairo Univ. Abdel – Rahman, G.A., 2001. W ater requirements of sugar beet under Ras Sudr condition. Desert Inst. Bull., Egypt, 51(2): 377-393. Abo – Dahab, A.M., R.S. El- Dabh, M.M. El Bagoury and S.H. El Kashlan, 1984. Effect of plant density on growth, yield and constituents of Roselle (Hibiscus sabdariffa, L.). Proc. the First Arab Conf. of Medicinal Plants Cairo. Nov. Allen, R.G., L.S. Pereira, D. Raes and M. Smith, 1998. Crop evapotranspiration, guidelines for computing crop water requirements. Irrig. & Drain. Paper, No. 56, FAO, Rom, Italy. Babatunde, F.E. and A.L.E. Mofoke, 2006. Performance of Roselle (Hibiscus sabdariffa L) as Influenced by Irrigation Schedules. Pakistan J. of nutrition, 5(4): 363-367. Balyan, S.S., D.K. Chowdhary and B.L. Kaul, 1990. Response of celery to different row spacing. Indian Perfumer, 34(2): 168-170. Du, C.T. and F.J. Francis, 1973. Anthocyanins of R o se lle . D ep t. F o o d S c i. T echn. U niv. Massachusetts, Amherst. Mass. J. Food Sci., 38 (5): 810–812. El- Barkoki, M., B.M. M ansour, M. W aly and M. Khater, 1979. Preliminary studies on guar plants. Proc. 1st Agric. Conference of Muslim Scientists, 97-114. 31 J. Appl. Sci. Res., 5(1): 21-32, 2009 21. Shalaby, A.S. and A.M. Razin, 1989. Effect of plant spacing on the productivity of Roselle (Hibiscus sabdariffa, L.) grown in newly reclaimed land. National Research Center. Dokki, Cairo, Egypt, J. Agro. and Crop Sci., 162: 4: 256-260. 22. Sobhan, M.A. and M. Husain, 1978. Effect of different plant densities on seed yield of (Hibiscus sabdariffa, L.). Altissima, Bangladesh Jute Res. Inst. Dacca Bangladesh J. Jute and Fiber Res., 3 (1-2). 23. Talha, M., M.A. Aziz and E.M . Toni, 1980. The combined effect of irrigation intervals and cycocel treatment on Pelargonitum graveolens. II- Evapotranspiration and water economy. Egypt. J. Soil Sci., 20(2): 121-125. 24. Yaron, B., E. Danfors and Y. Vadia, 1973. Arid zone irrigation. Spriger- Verlag Berlin Heileberg, New York. 32