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435 Journal of Applied Sciences Research, 9(1): 435-443, 2013 ISSN 1819-544X This is a refereed journal and all articles are professionally screened and reviewed ORIGINAL ARTICLES Quality Characteristics of Cantaloupe Seed Oil and Cookies Substituted with ground Full fat and Defatted Seeds. Hanan, M.A. Al-Sayed Food Sci. Dept., Fac. of Agric., Ain Shams Univerisity, Shoubra El- Kheima, Cairo, Egypt ABSTRACT Some physicochemical characteristics and fatty acids profiles of cantaloupe seed oil as non conventional source were investigated in this study. Quality characteristics of cookies substituted wheat flour with different levels (5, 10, 15, 20, and 25) of full fat and defatted ground cantaloupe seeds were also considered. It was found that ground cantaloupe seeds contained 29.47% oil and 20.06% protein. Moisture, ash, crude fiber and carbohydrates contents were 5.79%, 3.84 %, 31.22 % and 9.62%, respectively. Cantaloupe seed oil is liquid at room temperature (25ºC) and has yellow a color with agreeable flavours. Seed oil showed a refractive index 1.4740, low free fatty acids as oleic acid 0.22% and low peroxide value 1.08 meq / kg oil. Cantaloupe seed oil had a high level of vitamin E (1771.55 μg/100g), and high oxidative stability index (OSI) at 100 ºC (12 h) The cantaloupe seed oil contained 14.44% saturated fatty acids, with the major one being palmitic acid (9.38%) followed by stearic acid (4.69%).While, it was high in unsaturated fatty acids 85.56%. Linoleic acid is the major fatty acids in the cantaloupe seed oil (66.15 %) and oil was very poor in linolenic acid (0.28%). The moisture content in cookies samples increased with increasing of the substitute level with full fat and defatted ground cantaloupe seeds. Also, all cookies samples substituted wheat flour with 20% defatted ground cantaloupe seeds hadmaximum w idth (5.60 cm) followed by (5.56cm). The higher thickness (0.81cm) was recorded for full fat ground cantaloupe seeds at20 %.while, the maximum value of spread ratio (7.56) was observed in cookies substituted flour with 10% defatted ground cantaloupe seeds. The results revealed that 15% substituted wheat flour with full fat and defatted ground cantaloupe seeds produced acceptable cookies which were not significantly different from the control sample. Key words: Cantaloupe seed, oil, full fat, defatted, substituted, quality characteristics, cookies. Introduction One of the most common problems in food processing is the disposal of the sub products generated. This waste material produces ecological problems, (Hussein et al., 2011). In the food processing industry, edible portions of fruits are processed into products such as puree, canned slices, juice and pickles, whereas, seeds often will be discarded as waste since it is not currently utilized for commercial purpose,(Ajila et al., 2007).Seeds are promising source of useful compounds because of their favorable technological or nutritional properties,(Schieber et al., 2011) A lot of work has been carried out on analysis of seed oils by number of workers, primarily because of extensive demands for oils both for human consumption and for industrial applications; consequently there is an increasing need to search for oils from non-conventional sources to augment the available ones and also to meet specific application, (Kyari, 2008).Increased attention has focused on the utilization of by–products and wastes from food processing as well as, under utilized agricultural products. Obviously, such utilization would contribute to maximizing the available resources and result in the production of various products for food or feed (Evangelos, 1986). Vegetable oils are essential in meeting global nutritional demands and are utilized for many food and other industrial purposes. The conventional sources of vegetable oil no longer meet the ever increasing demands of domestic and sectors, (Idouraine et al., 1996). Therefore, the need exists to look for other sources to supplement the supplies. Conventional oil seeds are of much concern to cope this challenge. More recently, research activities have focused on examining and characterizing new sources of edible oil ,( Gohari-Ardabili et al., 2011). Esuoso et al., (1998), reported that seeds of some species of cucurbitaceae can be the edible oil sources to meet the increasing demands for vegetable oil. Many, cucurbitaceae produce seeds, rich in oil and protein. Although none of these oils has been utilized on as industrial scale, many are used as cooking oil in some countries in Africa and the Middle East ( Sawaya et al., 1983). Corresponding Author: Hanan, M.A. Al-Sayed, Food Sci. Dept., Fac. of Agric., Ain Shams Univerisity, Shoubra ElKheima, Cairo, Egypt. E-mail: [email protected] Tel: 01001864813 or 01061990654 436 J. Appl. Sci. Res., 9(1): 435-443, 2013 Cantaloupe (Cucumis melo var. cantalupensis), referred only to the non-netted and it is the most popular variety of melon in the USA.It is one of the most fruit crops worldwide due to its pleasant flavour and nutritional value. Cantaloupes are a diverse group of fresh, dessert fruits (Vouldoukis et al., 2004) .Melon seeds may be eaten as seeds or as fried cake prepared from milled seeds, (Okigbo, 1984). Ubbor and Akobundu, (2009), used of composite flour from watermelon seed, cassava and wheat in the production of cookies. Cookie is chemically leaved production, also known as biscuit. Generally the term biscuit is used in the European countries and cookies in the USA. Biscuits and biscuit like products have been made and eaten by man for centuries. Cookies are small round and flat cakes. Cookies are ideal for nutrient availability, palatability, compactness and convenience. They differ from other baked products like bread and cakes because of having low moisture content comparatively free from microbial spoilage and long shelf life of the product ( Hosney, 1986 ; Wade,1988 and Oyewole et al., 1996) Shams El-Din and Yassen, ( 1997), used guava seeds in cookies preparation as wheat substitute. Also, Atuonw and Akobundu, (2010), studied the chemical, nutritional and supplementary potential of defatted pumpkin seed flour biscuit making. The aim of this presented investigation was to evaluate some physicochemical characteristics and fatty acids profiles of cantaloupe seed oil as non conventional source. In addition, to determine the quality characteristics of cookies substituted wheat flour with different levels of full fat and defatted ground cantaloupe seeds. Materials and Methods Materials: Plant materials: Full ripened fresh cantaloupe fruits (Cucumis melo var.cantalupensis ) was obtained from the local market , Cairo, Egypt. The seeds were cleaned and washed of any adhering residue and dried at 40º C in a drying air oven for 24 h. Dried seeds were ground in a laboratory mill (ground full fat seeds). Cookies ingredients: Sucrose (commercial grade), shortening, fresh whole egg, baking powder (sodium bicarbonate and cream of tartar) were purchased from local market .wheat flour (72% extraction) was obtained from the Cairo south company of milling (El-Haram Milling). Methods: Extraction of cantaloupe seed oil: The dried, clean cantaloupe seeds were finely ground (full fat cantaloupe seed) and soaked in hexan as a solvent for 24h twice in dark place with gentle shaking at room temperature. Solvent was collected and evaporated under vacuum in rotary evaporator and the obtained oil was dried over sodium sulfate anhydrous, filtered and kept in brown glass till analysis. The residue was collected and dried at 40º C and was used as ground defatted cantaloupe seeds Preparation of cookies: Cookies samples were prepared according to the modified method of Sukhcharn et al., (2008).The formula used for cookies preparation included 100 g wheat flour different proportion of ground full fat or defatted seeds and wheat flour presented in Table (1), 30g sugar, 35g shortening, 1g sodium chloride, 0.6g baking powder and 5 ml water to make required consistency of dough. Cookie dough was made in a laboratory mixer (Moulinex). Shortening and sugar was creamed in a mixer for 2min at slow speed. Dough water containing the sodium chloride was added to the resulting cream and mixed for 5min at high speed to obtain a homogeneous mixture. Finally, wheat flour containing various proportions of ground full fat or defatted cantaloupe seeds, with baking powder was added and mixed for 3min at medium speed. The cookies dough was rested for 30 min then sheeted, cut, transferred to a lightly baking tray and baked at 205ºC for 12 min in LUXELL air oven model LX- 3575 made in Turkey. The baked cookies were cooled at room temperature before analysis. 437 J. Appl. Sci. Res., 9(1): 435-443, 2013 Table 1: Treatments used for preparation cookies substituted wheat flour with cantaloupe seeds. Treatments Cookies Substitution Wheat flour Full fat cantaloupe seeds (%) Control Zero 100 - Defatted cantaloupe seeds - 1 2 3 4 5 5 10 15 20 25 95 90 85 80 75 5 10 15 20 25 - 6 7 8 9 10 5 10 15 20 25 95 90 85 80 75 - 5 10 15 20 25 Analytical methods: Physicochemical characteristics of cantaloupe seed oil: - The oil percentage of cantaloupe seeds was calculated according to (Tahira, et al., 2007), on the basis of following formula:Percent oil in cantaloupe seeds (%) = weight of oil (g) / weight of sample (g) x 100 - Refractive Index at (25ºC), free fatty acid and peroxide value of cantaloupe seed oil were determined using the official method of A.O.A.C (2007). - Oxidative stability of cantaloupe seed oil was measured at 100 ºC by the Rancimat method using the method described by Mendz et al., (1997). - Vitamin E in cantaloupe seed oil was determined using HPLC system according to Pyka and Sliwiok,(2001). - Fatty acids of cantaloupe seed oil were determined using gas chromatography (Hewlett Packard 6890N, Agilent Technologies, USA) were determined according to the method described in A.O.A.C (2007). Chemical composition of ground cantaloupe seeds and cookies samples: Chemical composition of ground cantaloupe seeds and cookies samples including the contents of moisture, ash, crude protein, crude fat and crude fiber were determined according to A.O.A.C (2007). Carbohydrates were determined by difference. Physical characteristics of cookies sample: The cookies samples width, thickness and spread ratio were determined according to A.A.C.C (1995). Cookie width was measured by placing 6 cookies edge- to-edge to get the average width in centimeters. Cookie thickness was measured by stacking 6 cookies on top of each other. Width divided by the thickness gave the spread ratio. Sensory evaluation of cookies samples Ten panelists from the staff members of Food Science Department, Faculty of Agriculture, Ain Shams University were asked to score the quality attributes of each cookie sample. Appearance, color, texture, flavour, Crispness and overall acceptability were judged on a scale of 10 according to Meilgaard et al., (1999). Statistical analysis: Data were expressed as the mean + standard error (SE) of three replicates. The experimental data were analyzed using Analysis of Variance and Duncan, Multiple ranges at (p ≤ 0.05). The data were analyzed according to User’ Guide of Statistical Analysis System. (SAS, 2004). 438 J. Appl. Sci. Res., 9(1): 435-443, 2013 Results and Discussion Chemical composition of ground cantaloupe seeds: Chemical composition of ground cantaloupe seeds are presented in Table (2).The ground cantaloupe seeds contained 5.79± 0.05 % moisture, compared favorably with 4.85,5.21 and 6.10 reported for Colocynthis citrullus from Akure, Cucumeropsis edulis and prumus amygdalus seeds flour respectively (Akpambang et al., 2008). The protein content of cantaloupe seeds, 20.06 ±0.48% found in this study (Table 2), was in good agreement with those indicated by (Ajayi et al., 2006), for cashew nuts (22.8%),cotton seed (21,9%) and higher than those of sesame (18.7%) and animal proteins(16.0-18.0%) such as lamb ,fish and beef. Ash contents which are indicates of mineral contents was 3.84 ± 0.04% for cantaloupe seeds (Table 2) this value, however, compared favorably with those reported for Pruns amygdalus (3.34%) (Akpambang et al., 2008) and water melon seeds which ranged between 2.31% to 3.76 %( Acar et al., 2012).On other hand, plant seeds are rich source of lipids. The oil contents of cantaloupe seeds was found to be 29.47 ±0.61% (Table 2), this value fell in the range reported for different species of cucurbita (9.8-52.1%) (Stevenson et al., 2007).Also the oil content of cantaloupe seeds in the present study was found to exceed ,or be comparable to, that of some common edible oils such as cottonseed (22-27%) safflower (30-35 %), soybean (18-22%)and olive (12-50%) (Nichols and Sanderson, 2003).Therefore, the cantaloupe seeds can be consider as a potential source of vegetable oil for domestic and industrial purposes. Cantaloupe seeds characterized by high level of crude fiber content (31.22 ± 0.67 %)and this value was in good agreement with those obtained for hulled cantaloupe seeds Acar,( 2012) On other hand, carbohydrate contents was calculated to be 9.62 ± 0.09% .This value was higher than reported by Lazos,(1986) for pumpkin(5,6%). Table 2: Chemical composition of ground cantaloupe seeds. Constituents Moisture Crude Protein Ash Crude Fat Crude fiber * Carbohydrates Means of three replicates. ± Standard Error. * Carbohydrates were calculated by difference % 5.79 ± 0.05 20.06 ±0.48 3.84 ± 0.04 29.47 ±0.61 31.22 ± 0.67 9.62 ± 0.09 Some physical and chemical characteristics of cantaloupe seed oil: Physical properties of lipids derive directly from their chemical structure and functional groups and greatly influence the functions of lipids in foods and the methods required for their manipulation and processing. They can also be used to assess the purity or quality of lipid material in reference to know standards or preferred characteristics (Nichols and Sanderson, 2003). Some physicochemical characteristics of cantaloupe seed oil are summarized in Table (3),and results show that the percentage cantaloupe seed oil yields was 29.47%,this value fell in range(18-30%) reported for the olive seed, cotton seeds and soy bean by Olawale and Sc,(2010). Cantaloupe seed oil was liquid at room temperature (25ºC) and has yellow a color with agreeable flavours. Refractive index is used by most processors to measures to measure the change in unsaturation as the fat or oil is hydrogenated. The refractive index of oils depends on their molecular weight ,fatty acid chain length, degree of unsaturation ,and degree of conjugation (Nichols and Sanderson, 2003).The cantaloupe seed oil showed a refractive index of 1.4740.This value in the range reported for the pumpkin seeds oil(1.466-1.474)and it was lower than that reported for sunflower and olive oils; higher than that for palm, palm kernel and coconut oils; and within range reported for canola ,rape seed and corn oils(Nichols and Sanderson, 2003). The free fatty acids as oleic acid in the cantaloupe seed oil was o.22% and it was considerably lower than reported in pumpkin seed oil 0.39 % (Gohari-Ardabili et al ., 2011).The seed oil generally had low acid values and corresponding low levels of free fatty acid in the oils which suggests low level of hydrolytic and lipolytic activities in the oils. Thus, the cantaloupe seed oil could be good sources of raw materials for industries (Obasi et al., 2012).Also, in Table (3), cantaloupe seed oil had low peroxide value 1.08meq / kg oil compared favorably with the literature values 1.5308 meq / kg oil for melon Colocynthis citrullus (Obasi et al., 2012), 7.6 meq / kg oil for Citrullus lanatus var.citroides (Acar et al., 2012) and 0.92 meq / kg oil for rice oil (Tahira, et al ., 2007). The low peroxide value of cantaloupe seed oil is indicative of low level of oxidative rancidity of the oil and also suggests the presence or high levels of antioxidant. The oil oxidative stability index (OSI) is a criterion of oxidative stability of the oils and fats defined as the hours for an oil sample to develop a measurable rancidity (Gohari-Ardabili et al ., 2011).The oxidative stability index (OSI) at 100 ºC of cantaloupe seeds oil examined in this study was 12 h. 439 J. Appl. Sci. Res., 9(1): 435-443, 2013 Tocoppherol (vitamin E) homologues are phenolic antioxidants that occur naturally in vegetable oils and provide some protection against oxidation by termination free radicals. The determination of tocopherol homologues in the kernel is important owing their antioxidative effect and their positive nutrition influences in human metabolism as biological antioxidants (Yoshida et al., 2006). As shown in Table (3), the cantaloupe seed oil had a high level of vitamin E (1771.55 μg/100g), which be expected to contribute good oxidative stability of the cantaloupe seed oil during storage and processing. Table 3: Some physicochemical characteristics of cantaloupe seed oil. Characteristics Percentage (% )oil yield State at (25ºC) Color Odor Refractive index at (25ºC) Free fatty acid % (as oleic acid) Peroxide value (meq / kg oil) *Oxidative stability (hr) at 100 ºC Vitamin E (μg / 100g) * Rancimat method Values 29.47 Liquid Yellow Agreeable 1.4740 0.22 1.08 12.0 1771.55 Fatty acids composition of cantaloupe seed oil: Table (4), demonstrates that, four major fatty acids, namely linoleic, oleic, palmitic and stearic were found in the cantaloupe seed oil and they constituted 99.05% of all their fatty acids . The cantaloupe seed oil contained 14.44% saturated fatty acids, with the major one being palmitic acid (9.38%) followed by stearic acid (4.69%).While, it was high in unsaturated fatty acids with a total content of 85.56% .This total content of the unsaturated fatty acids was closer to that of other reported in pumpkin seeds oil 80.70%.(Gohari-Ardabili et al., 2011).Linoleic acid (C18:2) is the major fatty acids in the cantaloupe seed oil (66.15 %) and it is an excellent source of the linoleic acid. The linoleic acid content of cantaloupe seed oil is similar to that of those of corn, cottonseed, sunflower, soy bean and sesame oils (linoleic acid is the most abundant) (Fokou et al., 2009).On other hand, cantaloupe seed oil was very poor in linolenic acid (0.28%). Though, linolenic acid an omega-3 fatty acid with positive health effects, it easily oxidized and it is undesirable in edible oils because of the off – flavours and potentially harmful oxidation products formed, Warner and Gupta,(2003).Also, the level of other fatty acids in the cantaloupe seed oil was very low similar to the results reported in the literature (Stevenson et al., 2007). Table 4: Fatty acids composition of cantaloupe seed oil. Fatty acids Palmitic acid C16:0 Palmitoleic acid C16:1 Margaric acid C17:0 Stearic acid C18:0 Oleic acid C18:1 Linoleic acid C18:2 Linolenic acid C18:3 Arachidic acid C20:0 Eicosanoic acid C20:1 Behenic acid C22:0 Saturated fatty acids Unsaturated fatty acids % 9.38 0.17 0.08 4.69 18.83 66.15 0.28 0.18 0.13 0.11 14.44 85.56 Quality characteristics of cookies samples: Chemical composition of cookies samples: Chemical composition of cookies substituted wheat flour with full fat and defatted ground cantaloupe seeds are presented in Table (4).The results show that the moisture content of cookies samples increased with increasing the substitute levels with full fat and defatted ground cantaloupe seeds than control sample. The control sample showed the lowest values of moisture content 3.90%.Meanwhile, cookies substituted wheat flour with 25% defatted ground cantaloupe seeds had significant higher moisture content (6.48%) followed by cookies substituted wheat flour with 20% defatted ground cantaloupe seeds(5.89%). The increases in moisture content of cookies substituted wheat flour with defatted ground cantaloupe seeds may be due to the ability of high dietary fiber content in defatted cantaloupe seed to absorp water when compared to full fat. This observation is agreed with those reported by Abdalla, (2007); Ajila et al.,(2008); Aziah and Koma,(2009)and 440 J. Appl. Sci. Res., 9(1): 435-443, 2013 Ajila et al.,(2010). Also results showed that cookies substituted wheat flour with full fat and defatted ground cantaloupe seeds had higher content of ash, protein and fat than that of cookies (control sample). Cookies substituted wheat flour with 25% defatted ground cantaloupe seed had higher of ash and protein content (1.89 and 13.22), respectively, followed by cookies substituted wheat flour with 20% defatted ground cantaloupe seeds (1.55% and 12.67). Whereas, fat content were higher in cookies substituted wheat flour with 25% full fat ground cantaloupe seeds (24.01%) followed by cookies substituted wheat flour with 20% full fat ground cantaloupe seeds (22.95%) as compared with cookies substituted wheat flour with all levels of defatted ground cantaloupe seeds and control sample .Meanwhile, Cookies (control sample) and cookies substituted wheat flour with 5% defatted ground cantaloupe seeds had higher contents of carbohydrates (67.51% and 66.14%), respectively. Table 4: Chemical composition of cookies substituted wheat flour with full fat and defatted ground cantaloupe seeds. Treatments Substit. Moisture Ash Protein (%) Fat Carbohydrates (%)* (%) (%) (%) (%) Control Zero 3.90 h 0.76 i 9.23 i 18.60 k 67.51 a Full fat cantaloupe seeds 5 4.60 fg 0.92 h 9.64 h 20.62 e 64.22 c 10 4.73 f 1.07 f 9.92 g 21.71 d 62.57 e 15 5.14 e 1.25 e 10.32 f 22.76 c 60.53 f 20 5.30 d 1.36 d 10.80 e 22.95 b 59.59 g 25 5.54 c 1.46 c 11.20 d 24.01 a 57.79 i Defatted cantaloupe Seeds 5 4.48 g 1.01 g 9.61 h 18.76 j 66.14 b 10 4.53 g 1.32 d 10.83 e 18.94 i 64.38 c f c c h 15 4.68 1.45 11.64 19.33 62.90 d 20 5.89 b 1.55 b 12.67 b 19.59 g 60.30 f 25 6.48 a 1.89 a 13.22 a 19.91 f 58.50 h * : Mean in a column showing the same letter are not significantly different (P ≤ 0.05). * Carbohydrates were calculated by difference. Some physical characteristics of cookies samples: Physical characteristics of cookies are important from both consumers as well as manufactures point of view. The effect of substituted wheat flour with 5,10,15,20 and 25% full fat and defatted ground cantaloupe seeds in physical characteristics was studied and the data are presented in Table (5). The results showed that all full fat and defatted ground cantaloupe seeds treatments caused significant increase in cookies width as compared with 5.26 cm for control and 5% of full fat and defatted ground cantaloupe seeds (5.25 cm and 5.32 cm), respectively. It clear that cookies substituted wheat flour with 20% defatted ground seeds had maximum width (5.60 cm) followed by (5.56 cm) without significant difference with15% and 20% . While minimum width (5.23 cm) was observed in cookies substituted wheat flour with 5% defatted ground cantaloupe seeds without significant difference with control sample. It also, clears that cookies substituted wheat flour with defatted ground cantaloupe seeds up to 5% and full fat ground cantaloupe seeds at all levels resulted in significant increase in thickness when compared with 0.72cm for control. The higher thickness (0.81cm) was recorded for full fat ground cantaloupe seeds at 20 %. The observation is agreement with those obtained by (Ajila et al.,2008). On other hand, there was a decreasing trend in the spread ratio of cooking with proportionate increase of substitution with full fat and defatted ground cantaloupe seeds. Whereas, the maximum value of (7.56) for spread ratio was observed in cookies substituted flour with 10% defatted ground cantaloupe seeds. While, minimum value (6.86) in cookies substituted flour with 20%full fat ground cantaloupe seeds. The results of present study are quite close to the observation reported by Sekhon et al., (1997). The full fat and defatted rice bran were blended in wheat flour at 5, 10 and 15% to prepare cookies. Spread cookies decreased with the addition of rice bran. Sensory evaluation of cookies samples: Sensory evaluation results of the cookies samples are presented in Table (6).The result showed that no significant differences were observed between cookies substituted wheat flour with 5%,10% and 15% full fat and defatted ground cantaloupe seed and control sample in all sensory properties. Meanwhile, cookies substituted wheat flour with up to 15% full fat and defatted ground cantaloupe seeds had significant lower value for appearance, color, texture, flavour, crispness and overall acceptability. Results in Table (6) and Fig (1) revealed that 15% substituted wheat flour with full fat and defatted ground cantaloupe seeds produced acceptable cookies which were not significantly different from the control sample. 441 J. Appl. Sci. Res., 9(1): 435-443, 2013 Table 5: Some physical characteristics of cookies substituted wheat flour with full fat and defatted ground cantaloupe seeds. Treatments Width (cm) Thickness(cm) Spread ratio (Substit. %) Control Full fat cantaloupe seeds Zero 5.26 d 0.72 de 7.30 ab 5 5.25 d 0.73 cde 7.20 ab 5.43 bc abcd 7.15 ab 5.56 a 0.79 ab 7.02 ab 20 5.56 a 0.81 a 25 5.48 ab 0.77 abcd 7.11 ab 5 5.23 d 0.70 e 7.49 a 10 15 0.76 6.86 b Defatted cantaloupe seeds ab 7.56 a 10 5.48 15 5.52 ab 0.75 bcde 7.37 ab 20 5.60 a 0.77 abcd 7.22 ab 25 cd abc 6.87 b 5.34 0.73 cde 0.78 * : Mean in a column showing the same letter are not significantly different (P ≤ 0.05). Table 6: Means scores of sensory properties of cookies substituted wheat flour with full fat and defatted ground cantaloupe seeds. Means* scores of sensory properties Treatments Substit. Appearance Color Texture Flavor Crispness Overall (%) acceptability Control Zero 8.8 a 9.0 a 8.8 ab 8.8 a 9.0 a 8.8 a Full fat cantaloupe seeds 5 9.0 a 9.0 a 9.0 a 9.0 a 9.0 a 9.0 a a a ab a a 10 8.6 9.0 8.8 8.6 8.6 8.8 a 15 8.6 a 9.0 a 8.8 ab 8.6 a 8.6 a 8.8 a 20 7.8 b 8.6 b 8.0 c 8.0 b 7.8 b 8.0 c 25 6.8 c 8.0 c 6.6 d 8.0 b 6.4 c 6.8 de Defatted cantaloupe seeds 5 9.0 a 9.0 a 9.0 a 9.0 a 9.0 a 9.0 a 10 8.4 ab 9.0 a 8.6 abc 9.0 a 8.6 a 8.6 abc 15 8.6 a 9.0 a 8.6 abc 8.8 a 8.4 a 8.6 abc 20 6.4 c 8.6 b 6.6 d 8.0 b 6.4 c 7.2 d 25 6.4 c 8.0 c 6.2 d 7.8 b 6.4 c 6.2 e * : Mean in a column showing the same letter are not significantly different (P ≤ 0.05). Fig. 1: Cookies substituted flour with full fat and defatted ground cantaloupe seeds. 442 J. Appl. Sci. Res., 9(1): 435-443, 2013 Conclusion: From the present research work, it was concluded that ground cantaloupe seeds was rich in oil and protein and, considering its fatty acid profile, it closes to cotton seed, corn, sesame, and sunflower and soybean oils. According to physiochemical characteristics of cantaloupe seeds oil it can be considered as a new and valuable source of edible oil. Also, it was concluded that substitution of wheat flour with full fat and defatted ground cantaloupe seeds till 15% is possible without adversely affecting sensory characteristics of cookies. Cantaloupe seeds substitution significantly improved the crud fiber, protein and ash content of the cookies. In addition, it could be further processed into therapeutic functional food products. References A.A.C.C., 1995. Approved methods of the American Association of Cereal Chemists.10th ed. Method 44-15A, 44-13, 08-01, 30-10, and 32-10.The Association. Inc. St Paul , MN. USA. Abdalla, A.E.M., S.M. 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