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T h e 2 0 1 4 ... C A R d i o vA s C u l... i n s T i T u T e ...
T h e 2 0 1 4 A n n u a l R e p o rt C ar d i o v a s c u l ar r e s e arc h Institute of vermont T he Cardiovascular Research Institute of Vermont is dedicated to reducing the incidence, morbidity, and mortality of heart and vascular diseases through improving prevention, diagnosis, and treatment. By fostering collaborations among departments at The University of Vermont and The University of Vermont Medical Center, the Cardiovascular Research Institute of Vermont encourages the critical thinking that challenges assumptions and promotes excellence in clinical practice. 6 car diovascu l ar r e search i nstitute of ve r mont • 2 0 14 a n n u a l r e p o r t Message from the Director The University of Vermont (UVM) and The University of Vermont Medical Center have a tradition of excellence in cardiovascular research—this report highlights important accomplishments in 2014. The Cardiovascular Research Institute of Vermont (CVRI) is broadly inclusive of all who engage in cardiovascular research at UVM and its Medical Center. Our mission is to foster cardiovascular research by supporting career development and by spotlighting outstanding accomplishments. David J. Schneider, M.D., F.A.C.C., F.A.H.A Director Cardiovascular Research Institute of Vermont Professor of Medicine University of Vermont College of Medicine Director of Cardiovascular Services University of Vermont Health Network Designated Distinguished Investigators of the CVRI highlight the legacy of cardiovascular research. Career development is augmented in multiple ways, including an Early Career Advisory Committee that is charged with guiding CVRI activity to support career development; travel awards to assist early career investigators so they may present research findings at scientific meetings; and invited speakers who enhance collaboration, stimulate new discoveries, and interact directly with early career investigators to inspire commitment to cardiovascular research. Progress toward the ultimate goal, improving care of patients with cardiovascular disease, is demonstrated by the many publications in cardiovascular research authored by investigators at our academic medical center. Much of this research was made possible by extramural funding for scientific discovery. As you read through these pages, I trust you will join me in appreciating the depth and breadth of cardiovascular research and in applauding the commitment of our investigators who continuously strive for excellence. Contents Board of DIRECTORS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Leadership council . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 cardiovascular research NEWS. . . . . . . . . . . . . . . . . . . . . . . . . . . 4 The Next Generation: Early career advisory committee . . . . 6 The Next Generation: Travel Awards. . . . . . . . . . . . . . . . . . . . . . . 8 distinguished investigators. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 Scholarly Events . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 research funding: Highlights. . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 research Publications: A sampling . . . . . . . . . . . . . . . . . . . . . . . 18 In Memory of Burton E. Sobel, M.D.. . . . . . . . . . . . . . . . . . . . . . . . 24 Contact. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 2 0 15 a n n ua l r e p ort 1 Board of Directors David J. Schneider, M.D., F.A.C.C., F.A.H.A., Director Professor of Medicine, University of Vermont College of Medicine Director of Cardiovascular Services, University of Vermont Health Network Director, Cardiovascular Research Institute of Vermont Dr. Schneider’s roles at the UVM Health Network and the Cardiovascular Research Institute provide him the opportunity to translate scientific advances into improved care of patients. His research focuses on thrombosis, platelet function, and fibrinolysis as they relate to atherosclerotic cardiovascular disease. His research efforts include the development of anti-thrombotic agents and novel methods to assess the risk of thrombosis and bleeding. From left: Drs. Schneider, Dauerman, Cipolla, Warshaw, Nelson, Bernstein and Cushman. Harold L. Dauerman, M.D., F.A.C.C. Professor of Medicine, Division of Cardiovascular Medicine, University of Vermont College of Medicine; Interventional Cardiologist, University of Vermont Medical Center Ira Bernstein, M.D. Professor and John Van Sicklen Maeck Chair of Obstetrics, Gynecology & Reproductive Sciences, University of Vermont College of Medicine; Medical Director of Women’s Services, University of Vermont Medical Center Dr. Dauerman’s research focuses on high risk atherosclerotic coronary syndromes and structural heart disease. He has investigated acute myocardial infarction pathophysiology with highly sensitive intracoronary imaging devices and applied systems-based approaches to optimize heart attack patient care; he has helped develop minimally invasive technologies to treat patients with aortic valve disease. He served as vice-chair of the American Heart Association Mission Lifeline program and is the Editor in Chief of Coronary Artery Disease. Dr. Bernstein served as Senior Associate Dean for Research at the UVM College of Medicine from 2009-2014, and was Director of Maternal Fetal Medicine and its fellowship program from 2003-2009. His research examines human integrative cardiovascular physiology and he has been funded from NIH for a series of projects examining pre-pregnancy determinants of preeclampsia. Dr. Bernstein has served on several study sections at NIH, including two years as the chair of Pregnancy and Neonatology. Marilyn J. Cipolla, Ph.D., F.A.H.A. Professor of Neurological Sciences, University of Vermont College of Medicine Mark T. Nelson, Ph.D., F.A.H.A. University Distinguished Professor and Chair of Pharmacology, University of Vermont College of Medicine Mary Cushman, M.D., M.Sc., F.A.H.A. Professor of Medicine, Division of Hematology/Oncology, University of Vermont College of Medicine; Director, Thrombosis and Hemostasis Program, University of Vermont Medical Center David M. Warshaw, Ph.D., F.A.H.A. Professor and Chair of Molecular Physiology & Biophysics, University of Vermont College of Medicine Dr. Nelson’s goal is to understand the control of smooth muscle and endothelial cell function by ion and calcium signaling. A major focus is to understand the control of brain microcirculation in health and small vessel disease. Research approaches cover the spectrum from molecular, cellular, intact tissue, whole organ and in vivo, and a number of genetic mouse models are used to unravel the control mechanisms. Dr. Nelson is internationally recognized for his cutting edge research. Dr. Cipolla’s research focuses on cerebral hemodynamics and cerebrovascular function under normal and pathologic conditions, including ischemic stroke and preeclampsia. Internationally recognized for her work, she is scientific advisor to the NIH and WHO and past President of the Perinatal Research Society. She is a Fellow and Established Investigator of the AHA and was named University Scholar in 2015. Dr. Warshaw’s research focuses on the structure and function of cardiac muscle proteins in normal and failing hearts. Genetic mutations to the tiny protein molecular motors that power the heart are the leading cause of sudden death in young athletes. He is an Established Investigator and Fellow of the American Heart Association. He has organized numerous international conferences and symposia, and was a Scientific Advisor for the NIH Nanomedicine Initiative. Dr. Cushman studies causes of cardiovascular-related diseases including heart disease, stroke, cognitive impairment and venous thrombosis. She uses biomarkers and genetic data from large epidemiologic studies where her lab serves as biorepository and analysis lab, and has authored over 400 publications. She is a Board Director of the American Heart Association and is Senior Guest Editor of Circulation. 2 car diovascu l ar r e search i nstitute of ve r mont • 2 0 14 a n n u a l r e p o r t Leadership Council Members of the Cardiovascular Leadership Council serve as ambassadors for the Cardiovascular Research Institute of Vermont (CVRI), its Board of Directors, Investigators and Faculty, in the overall effort to educate and engage Vermonters and the broader community in support of cardiovascular medicine. Mary Evslin is co-founder of NG Advantage LLC, a Vermont-based company that delivers natural gas to large industrial plants not on a gas pipeline. Previously, she was a founder of Marketing and Customer Success at ITXC, where she developed and led the company’s global marketing efforts from start-up through IPO and secondary offering. Earlier in her career she worked for then-U.S. Representative Jim Jeffords. She is on the Board of Trustees of Champlain College and the Vermont/New Hampshire Chapter of the American Red Cross, and was the first chair of the Board of Directors of the Vermont Telecommunications Authority. Mary lives in Stowe with her husband, Tom. Mark Ray is the Director of Public Relations at KSV, a Burlington marketing communications firm. A graduate of Middlebury College, he honed his skills in NYC at three top PR firms before returning to Vermont in 1994. In 2001, Mark co-founded an annual event in memory of his father, a cyclist who died of heart disease, and has raised over $120,000 for heart disease research and education. Mark served as vice chair of the Make-A-Wish Foundation’s Vermont board in 2013, and has also served on the boards of the American Heart Association in Vermont and the Middlebury College Alumni Association. Mark lives with his wife and two children in Shelburne. Peter Gibbs is a Vice President at Engineering Ventures PC, a civil and structural engineering firm in Burlington. Previously, he owned his own engineering firm based in Westport, N.Y., and has been a practicing site and civil engineer for over 30 years. Peter holds a Master of Engineering from Rensselaer Polytechnic Institute, and Bachelor of Science degrees in both Ocean Engineering and Civil Engineering from the Florida Institute of Technology. He is a member of the Construction Specification Institute, a volunteer with Engineers Without Borders, and a licensed U.S. Coast Guard Captain. Peter and his wife Lauren reside in Shelburne. Patrick Robins is founder and chairman of The SymQuest Group, Inc., a regional technology services company. Previously, Pat was Chairman and CEO of McAuliffe, Inc. A graduate of St. Michael’s College and the Tuck School of Business at Dartmouth, Pat serves as a Director at Merchants Bancshares, is a managing member of Sideronics LLC, and Director of Butternut Mountain Farms, a national distributor of maple products. He holds board positions on the Downtown Burlington Development Corp., the VNA, Cynosure, Inc., the UVM Medical Center, the Vermont Chamber of Commerce, and the Vermont Land Trust. Pat and his wife, Lisa Schamberg, reside in Burlington. Paul Millman is a founder and president of Chroma Technology Corp., one of the most important suppliers of optical filters and mirrors to the world’s biotech industry. He is a graduate of the New School and the Antioch New England Graduate School and serves as director of the Vermont Business Roundtable and Valley Net. He is on the Steering Committee of ReThink Health in the Upper Valley and president of the Westminster (Vermont) Fire and Rescue Association. He is also a former director of Vermont Business for Social Responsibility and former chair of the Vermont Employee-Ownership Center. He lives in Westminster with his partner Wendy Cross. Glen Wright is a certified public accountant, serving as Managing Partner at KPMG LLP in Burlington until 2002. Since then, he has continued his work as a business and tax consultant in Burlington and Reddick, Florida. Glen is Chairman of the Board of Cynosure, Inc., whose mission is to provide financial support for economic development in Chittenden County through investment and management of real estate projects. He has been a board member of the Flynn Theatre for the Performing Arts, the Vermont Business Roundtable, the YMCA, the UVM Medical Center Foundation and Chittenden Bank. Glen and his wife Rosemarie, divide their time between South Hero and Florida. www . uvm . edu / medicine / cvri 3 Cardiovascular Research News Promising New Treatment for Heart Rhythm Disorder C ardiologist and heart rhythm specialist Peter Spector, M.D., is on a mission to improve the cure rate for a form of the most common heart rhythm disorder – atrial fibrillation (AF) – and has already earned a patent as he works towards this goal. Roughly six to eight million cases of AF exist in the U.S., but despite its prevalence, medications only work in about 45 percent of patients. An alternative to medication exists; a procedure – called catheter ablation – is effective in about 75 percent of patients with intermittent AF, but the technique works poorly in the majority of AF patients, who suffer from a chronic form of the condition. Spector’s three-year project – funded by a $1 million grant from Vermont residents Tom and Mary Evslin after Mr. Evslin was treated for a heart condition at The University of Vermont Medical Center – has yielded innovative technology that offers a potential new approach to catheter ablation of AF and led to creation of an impressive intellectual property portfolio covering the catheters, signal processing algorithms and other aspects of his research. He was awarded the first of these U.S. patents in November 2014, and as of January 2015, a second patent application has been allowed. Through all of these efforts, Spector and colleagues hope to help the many patients suffering from chronic AF to maintain a regular heart rhythm, thus reducing their risk of stroke and providing them with a significantly higher quality of life. “If our therapy is even 10 percent more effective, it will help an enormous number of people,” says Spector. SPARK-VT Awards Support Innovation S PARK-VT, launched at UVM in 2012, brings the experience and insight of a panel of distinguished and successful entrepreneurs to the critical evaluation of promising innovative applications of new knowledge. Those adjudged to be most meritorious receive start-up funding and milestone evaluation by the panel. In 2014, UVM Associate Professor of Medicine Jeffrey Spees, Ph.D., earned a SPARK-VT award for his research on Cell-Kro, a grafting agent composed of insulin and a peptide derived from Connective Tissue Growth Factor. Cell-Kro has been shown in early studies to improve the adhesion, proliferation, survival, and migration of cardiac stem cells grafted to a heart injured from a heart attack. In 2013, Associate Professor of Medicine Markus Meyer, M.D., earned an award for his project to develop a fast and inexpensive way to test for heart function: A simple device to administer a small dose of nitrogen to a patient and time its flow through the body. Meyer completed follow-up studies on the heart function monitor, created a startup company to support his venture, and submitted a Small Business Innovation Research (SBIR) grant. 4 car diovascu l ar r e search i nstitute of ve r mont • 2 0 14 a n n u a l r e p o r t Translating Groundbreaking Cardiovascular Research Into Improved Regional Care A CVRI Supports AHA Go Red for Women T he American Heart Association’s Vermont Go Red for Women Luncheon attracts 500 attendees each year to the organization’s Heart Month event held in Burlington every February. The University of Vermont College of Medicine, University of Vermont Medical Center and Cardiovascular Research Institute of Vermont co-sponsor the event, which includes a morning panel discussion, silent auction, and luncheon keynote and survivor presentations. UVM Professor of Surgery and Cardiothoracic Surgeon Frank Ittleman, M.D. (shown above), was named Vermont’s 2014 recipient of the Crystal Heart Award, which recognizes an individual who has contributed to the mission of the American Heart Association and American Stroke Association. Vermont AHA Board Chair Mary Cushman, M.D., M.Sc., presented the award to Dr. Ittleman. procedure tested during a clinical trial at the University of Vermont to replace heart valves using catheters instead of open heart surgery received approval in July 2014 from the Food and Drug Administration for use in two categories of patients. The American Heart Association and the American College of Cardiology have also added Transcatheter Aortic Valve Replacement (TAVR) to their guidelines. Patients from Vermont and northern New York who had failing heart valves but could not tolerate open heart surgery (the current standard of care for valve replacement) or were at high risk for complications or death during surgery were among the first in the country to receive this groundbreaking treatment. The UVM Medical Center was one of only 45 sites in the trial and has enrolled over 100 regional patients in research trials and registries studying and developing TAVR. During TAVR, an artificial valve is placed on a catheter and is advanced to the heart from a vessel in the leg or chest, similar to what happens when inserting a stent. When the new valve is released, it pushes the diseased valve out of the way and begins functioning immediately. “TAVR is truly a lifesaving treatment and we’re very pleased our heart team was involved in determining its effectiveness,” said clinical investigator Harry Dauerman, M.D. “It’s a great example of the value of having an academic medical center in our region – we not only have the expertise and resources to deliver the most advanced treatments, but we are also involved in developing them.” UVM has continued to take the lead in enrollment of high risk, off label patients in an ongoing national TAVR research registry and has now developed one of the highest volume clinical TAVR programs in our region. The Totman Medical Research Fund The Totman Medical Research Trust supports an interdepartmental, highly interactive research group focused on key aspects of brain artery function from molecules to clinical applications. The program is housed in the Department of Pharmacology, under the supervision of University Distinguished Professor and Chair Mark T. Nelson, Ph.D., who hosts an annual session with Fund leaders and researchers (above, in 2014) to hear presentations and share ideas. The Fund was established through the generosity of Ray Totman, a businessman from Malone, New York. After his death in 1988, a gift from his estate established the Ray W. Totman and Ildah Totman Medical Research Fund, to be used for scientific and medical research concerning Ischemic Cerebrovascular Disease. www . uvm . edu / medicine / cvri 5 The Next Generation: Early Career Advisory Committee W Sobel Visiting Professor Joseph Loscalzo, M.D., Ph.D., Chair of Medicine at Brigham & Women’s Hospital and Editor-in-Chief of Circulation, talks with Early Career scientists at the “Master’s Tea” during his visit to campus in March 2015. “You want your trainees to have the opportunity to meet people who are leaders in their field—it teaches them how to interact with scientists outside our institution. This can often initiate new collaborations, inspire new projects, and develop new mentors.” Mary Cushman, M.D., M.Sc. 6 car diovascu l ar r e search i nstitute of ve r mont • ith travel awards, research seminars, and an Early Career Advisory Committee available to them, junior investigators who are affiliated with the Cardiovascular Research Institute of Vermont (CVRI) have plenty of rich opportunities at their disposal. The Early Career Advisory Committee is at the heart of the CVRI support of the future of cardiovascular research and medicine. Its eight members, selected by application to the CVRI Board of Directors, represent multiple of departments and levels of expertise—they range from fourth-year medical students to early career faculty members in Microbiology, Molecular Genetics and Obstetrics, Gynecology and Reproductive Sciences. In addition to coordinating events such as Journal Clubs for their peers, committee members participate in the planning of the Visiting Professorships and report regularly to the CVRI Board on issues faced by their early career colleagues. The committee is shepherded by Mary Cushman, M.D., M.Sc., director of the Thrombosis and Hemostasis Program, professor of medicine in the Hematology/ Oncology Division, professor of pathology, and a member of the CVRI Board for whom mentoring has long been a professional passion. She says that inviting early career individuals to dinners with visitors and to professional development events where they are welcome to present their work and get feedback, is important on several levels. 2 0 14 a n n u a l r e p o r t From left to right: Sean McMahon, M.D., Abbie Johnson, Ph.D., Kara Klingman Landry, Kelley McLean, M.D., Michael Previs, Ph.D., Dawei Li, Ph.D., Mary Cushman, M.D., M.Sc., Nels Olson, Ph.D., and Patrick Hohl, D.O. 2014-15 Early Career Advisory Committee Patrick Hohl, D.O. 1st year fellow Medicine – Cardiology Kelley McLean, M.D. Assistant Professor Obstetrics, Gynecology and Reproductive Sciences Abbie Johnson, Ph.D. Postdoctoral Associate Neurological Sciences Sean McMahon, M.D. 1st year fellow Medicine – Cardiology Kara Klingman Landry, B.A. 4th year medical student UVM College of Medicine Nels Olson, Ph.D. Postdoctoral Fellow Pathology and Laboratory Medicine Dawei Li, Ph.D. Assistant Professor Microbiology and Molecular Genetics Michael Previs, Ph.D. Assistant Professor Molecular Physiology and Biophysics www . uvm . edu / medicine / cvri 7 The Next Generation: Travel Awards Travel awards were established to cover educational travel costs for anyone early in his or her career who is a primary presenting author of an abstract at a regional or national meeting. It’s an honor, and it also means that money that would otherwise have to be used to pay for them to participate can be earmarked for direct research costs instead. Since July 2014, thirteen $2,000 awards have been made, sending assistant professors, postdoctoral fellows, residents in internal medicine, and graduate and medical students to regional, national and international meetings. American Heart Association Epidemiology, Prevention, Lifestyle and Cardiometabolic Health 2015 Scientific Sessions American Society of Nuclear Cardiology 2014 Annual Scientific Sessions Boston, MA – September 18-21, 2014 Sean R. McMahon, M.D. Internal Medicine Resident, Department of Medicine Poster: Increased use of regadenoson in patients with abnormal troponin I in the era of sensitive troponin assays Baltimore, MD – March 3-6, 2015 Kristine Alexander, Ph.D., M.C.R. Postdoctoral Fellow, Department of Medicine Poster: Nonalcoholic fatty liver disease (NAFLD) and risk of incident cognitive impairment American Heart Association Scientific Sessions 2014 Markus Degirmenci, M.D. Internal Medicine Resident, Department of Medicine Poster: Dehydroepiandrosterone (DHEAS) and risk of stroke in black and white Americans: The REasons for Geographic And Racial Differences in Stroke (REGARDS) cohort Chicago, IL – November 7-11, 2014 Zubin Agarwal, M.D., M.P.H. Internal Medicine Resident, Department of Medicine Poster: Galectin-3 and risk of stroke: The REasons for Geographic And Racial Differences in Stroke (REGARDS) cohort Peter Durda Graduate Student, Department of Pathology Poster: Circulating soluble CD163 and risk of cardiovascular disease and all-cause mortality in older persons: the Cardiovascular Heart Study (CHS) Biophysical Society 59th Annual Meeting Baltimore, MD – February 7-11, 2015 Michael J. Previs, Ph.D. Assistant Professor, Department of Molecular Physiology and Biophysics Poster and Oral: Myosin-binding protein C corrects an intrinsic nonuniformity in cardiac excitation-contraction coupling 8 car diovascu l ar r e search i nstitute of ve r mont Kara Klingman Landry UVM Medical Student, Class of 2015 Poster: Association of stroke risk biomarkers with stroke symptoms: the REasons for Geographic And Racial Differences in Stroke (REGARDS) cohort • 2 0 14 a n n u a l r e p o r t Society for Reproductive Investigation 62nd Annual Scientific Meeting San Francisco, CA – March 25-28, 2015 Erin Morris, M.D. Fellow, Department of Obstetrics, Gynecology, and Reproductive Sciences 3 Posters: Prepregnancy blood pressure and history of first trimester loss contribute to birth weight and placental weight; Cerebral blood flow changes over pregnancy in women with a history of HELLP syndrome and those with prior preeclampsia; and Persistence of pregnancy-induced maternal uterine vascular remodeling in rodents Lindsay Howe, B.S. Graduate Student, Department of Obstetrics, Gynecology, and Reproductive Sciences Poster: Obesity, body fat distribution and cardiovascular function in young nulliparous women Carole McBride Graduate Student, Department of Obstetrics, Gynecology, and Reproductive Sciences 3 Posters: Factors influencing early pregnancy uterine arterial blood flow and resistance index; Maternal hypertension and major morbidity in infants born 22 to 29 weeks gestation; and Physical fitness and cardiovascular phenotype in young women Experimental Biology 2015 Boston, MA – March 28-April 1, 2015 Thomas A. Longden, Ph.D. Postdoctoral Fellow, Department of Pharmacology Poster: Unique ion channel property of brain capillary endothelial cells Yao Li Graduate Student, Department of Pharmacology Poster: Rho kinase regulates myogenic depolarization of cerebral parenchymal arterioles International Society for Stem Cell Research 2015 Annual Meeting Stockholm, Sweden – June 24-17, 2015 Krithika Rao Graduate Student, Department of Medicine Oral: CTGF-D4/LRP6 signaling promotes adult epicardial cell grafts after MI “The ability to underwrite expenses early in a young researcher’s career is crucial to his or her success, and we’re here to support our junior investigators.” Harold Dauerman, M.D. www www. .uvm uvm. .edu edu//medicine medicine//cvri cvri 99 Distinguished Investigators The Cardiovascular Research Institute of Vermont (CVRI) recognized six University of Vermont faculty in 2014 as Distinguished Investigators, recognizing the long-term high impact of their work in cardiovascular research. Appointed for a period of five years, the inaugural group was celebrated in April 2014. Russell Tracy, Ph.D. Professor of Pathology Dr. Tracy’s Laboratory for Clinical Biochemistry Research was the clearinghouse for the massive Exome Sequencing Project sponsored by NIH’s National Heart, Lung and Blood Institute (NHLBI). His own research was an integral part of the ESP’s HeartGO consortium, as well as the NHLBI’s Cardiovascular Heart Study and the National Center for Biotechnology Information’s Multi-Ethnic Study of Atherosclerosis. Tracy, interim senior associate dean for research, LCBR director, and 2009 University Scholar, is exploring genetic risk factors related to myocardial infarction and atherosclerosis, among other diseases. George Osol, Ph.D. Professor of Obstetrics, Gynecology and Reproductive Sciences Dr. Osol’s research is focused on vascular adaptations in pregnancy—in particular, the process of uterine artery remodeling in both normal and hypertensive pregnancies. Uterine vasculature undergoes more expansion during gestation than any other blood vessel in the human body ever does; insufficient remodeling or excessive constriction can result in preeclampsia and intrauterine growth restriction. Osol is program director for the NIH Center of Excellence in Women’s Reproductive Health Research and a 2010 University Scholar. His work has received NIH support for more than 25 years. 10 cardi o v a s c u l a r r e s e a r c h i n s t i t u t e o f v e r m o n t • 2 0 14 a n n u a l r e p o r t Kathleen M. Trybus, Ph.D. Professor of Molecular Physiology and Biophysics Joseph E. Brayden, Ph.D. Professor of Pharmacology Dr. Brayden’s laboratory investigates the ionic mechanisms of vasoconstriction, vasodilation, and communication between endothelial and smooth muscle cells in cerebral arterioles—with a focus on signaling pathways involved in cellular calcium regulation— using electrophysiological, optical, and molecular techniques. Brayden is also working to identify the roles of vascular endothelium and its many ion channels in cerebral and peripheral artery vasodilator function, and is a pioneer in research on the electrical properties of vascular smooth muscle. Dr. Trybus is engaged in the study of molecular motors and their cargo. Specifically, she has zeroed in on how myosin activity is regulated, and how it moves actin and produces force. Trybus is also interested in the mechanisms that cause mutations in smooth muscle actin to result in vascular disease— in particular, thoracic aneurysms and coronary artery disease—by expressing homogenous normal and mutant vertebrate actins in the baculovirus/insect cell expression system, and has followed the polymerization of single actin filaments in real time. Martin M. LeWinter, M.D. Professor of Medicine Broadly, Dr. LeWinter has trained his research eye on myocardial and ventricular functioning and remodeling in cardiomyopathy and heart failure. Specifically, he’s looking at the mechanoenergetics of the myocardium, diastolic left ventricular function, and the effect of diabetes and hypertension on myocardial function. LeWinter, a 2007 University Scholar, is director of the UVM Medical Center’s Heart Failure Program and principal investigator of a grant designating the University one of nine NIH-funded Regional Clinical Centers for heart failure research. Philip Ades, M.D. Professor of Medicine Dr. Ades’ father died of heart disease at a young age, and his own work is dedicated to ensuring that others don’t suffer the same fate. With a research and clinical spotlight on weight loss in obese coronary patients, Ades, who is director of Cardiac Rehabilitation and Prevention, teaches patients that exercise is medicine, but everyone needs a specific dose. His research, which has had consistent NIH funding since 1988, focuses on the treatment and prevention of disability in older patients with coronary artery disease and patients with chronic heart failure. www . uvm . edu / medicine / cvri 11 Scholarly Events The Cardiovascular Research Institute of Vermont (CVRI) brings outstanding scientists in cardiovascular medicine to The University of Vermont as Visiting Professors. These visits include a major lecture and a series of interactions with trainees and junior investigators. CVRI Visiting Professors Sobel Visiting Professor October 10, 2014 Honoring Burton E. Sobel, M.D., the Founding Director of the CVRI. Translational Stroke Research: A Personal Perspective Marc Fisher, M.D., M.Sc. Professor Emeritus of Neurology, University of Massachusetts Editor-in-Chief of Stroke March 26-27, 2015 November 7, 2014 Chair and Physician-in-Chief, Department of Medicine, Brigham and Women’s Hospital; Hersey Professor of the Theory and Practice of Medicine, Harvard Medical School; Editor-inChief of Circulation • Seminar: How to Write and Publish a Paper: Lessons Learned as an Author, Mentor, and Editor • Grand Rounds: Systems Pathobiology and Personalized Cardiovascular Medicine • Master’s Tea and dinner with the Early Career Advisory Committee Peripheral Artery Disease: Clinical Insights and Contemporary Treatments to Preserve Life and Limb Mark Creager, M.D. Professor of Medicine, Harvard Medical School; Senior Physician, Brigham and Women’s Hospital; President-Elect of the American Heart Association November 18, 2014 In Search of the Holy Grail: Where Next in Prediction of Preeclampsia? Leslie Myatt, Ph.D. Professor of Obstetrics and Gynecology, Director of the Center for Pregnancy and Newborn Research, University of Texas Health Science Center San Antonio March 5, 2015 Mitochondrial Dynamism and Heart Disease Gerald Dorn, Ph.D. Philip and Sima K. Needleman Professor and Associate Chair for Translational Research, Department of Internal Medicine, and Director of Center for Pharmacogenomics, Washington University May 8, 2015 Heart Failure Marvin Konstam, M.D. Professor of Medicine, Tufts University; Chief Physician Executive, The CardioVascular Center at Tufts Medical Center 12 car diovascu l ar r e search i nstitute of ve r mont The Inaugural Sobel Visiting Professor Joseph Loscalzo, M.D., Ph.D. Burton E. Sobel, M.D. Joseph Loscalzo, M.D., Ph.D. Alpert Visiting Professor Honoring Norman Alpert, Ph.D., Professor and Chair of the UVM Department of Molecular Physiology and Biophysics from 1966 to 1995. May 3-5, 2015 The Inaugural Alpert Visiting Professor Leslie A. Leinwand, Ph.D. Howard Hughes Medical Institute Professor of Molecular, Cellular and Developmental Biology, Chief Scientific Officer, BioFrontiers Institute, University of Colorado Boulder Events planned include a seminar “Translating Python Biology to Mammalian Hearts,” and Early Career Discussion Forum. • 2 0 14 a n n u a l r e p o r t Norman Alpert, Ph.D. Patent Activity New Patent Filed Jeffrey L. Spees, Ph.D. • Cell-Kro (C-terminal domain 4 of CTGF and Insulin) Ongoing Patent Activity Marilyn J. Cipolla, Ph.D. • Methods of treating diseases associated with PPARγ • Oxidized LDL as a biomarker for brain injury in preeclampsia David J. Schneider, M.D. • Compositions and methods for assaying platelet reactivity and treatment selection Peter Spector, M.D. • Method and systems for optimizing detection and treatment of atrial fibrillation • Methods and systems for mapping cardiac fibrillation • Methods and systems for optimizing lesion placement to minimize and treat cardiac fibrillation • Methods and systems for minimizing and treating cardiac fibrillation • Methods and systems for determining spatiotemporal variability for mapping cardiac fibrillation • Catheter systems and related methods for mapping, minimizing and treating cardiac fibrillation • Methods and systems for assessing cardiac fibrillogenicity • An interactive tissue model for simulating the electrical activity of excitable tissues Companies Formed selected scholarly Presentations Mary Cushman, M.D., M.Sc. Clinical Roles for Thrombophilia Testing: the 4Ps Approach Thrombosis and Hemostasis Summit of North America April 2014; Chicago, IL Saulius Butenas, Ph.D. Activation, Activity and Inactivation of FVIII in FVIII Products XXXI International Congress of the World Federation of Hemophilia May 2014; Melbourne, Australia Kathleen E. Brummel-Ziedins, Ph.D. Correlation of Bleeding and Thrombotic Phenotypes with Computational Modeling of Thrombin Generation 2014 7th Symposium on Hemostasis: Old System, New Players, New Directions May 2014; Chapel Hill, NC Harold L. Dauerman, M.D. Chair of Programming for the Clinical Cardiology Council and Scientific Sessions Programming Committee Member 2014 American Heart Association Annual Scientific Sessions November 2014; Chicago, IL Marilyn J. Cipolla, Ph.D. Targeting Parenchymal Arterioles for Treatment of Acute Stroke Brain Ischemia and Stroke (BIS) 2014 Conference December 2014; Rome, Italy Markus Meyer, M.D. • SimMedTec, LLC To develop a handheld circulation monitor www . uvm . edu / medicine / cvri 13 Research Funding: Highlights Understanding the causes and consequences of cardiovascular disease, from the molecule to the patient to populations to policy, drives a robust research enterprise at the University of Vermont, and represents a significant portion of the $81 million in funding received by the College of Medicine in 2014. Grant support comes from Federal, State, Corporate and Non-Profit sources; a sampling of recent awards is presented below. Government Funded Research: NIH, NHLBI, DMRDP, and other related awards NIH 13761 Systems Biology for Biological Responses to Severe Hemorrhage – UVM Co-PI: Kenneth Mann, Ph.D. and Kathleen Brummel-Ziedins, Ph.D. $1,503,734 Ad5.AC6 Gene Transfer for CHF: Use of Intracoronary AC6 Gene Therapy to Improve Symptomatic Heart Failure Local PI: Matthew Watkins, M.D. $26,000 HL-13-025 P01 HL095488-01 DMRDP Analysis and Characterization of TraumaInduced Coagulopathy Project 2 – The Role of Factor Xla in TIC Saulius Butenas, Ph.D. Project 6 – Analysis and Characterization of Trauma-Induced Coagulopathy PI: Kathleen Brummel-Ziedins, Ph.D. $23,999,991 K08 HL096841 Calcium Signaling in the Cerebrovascular Unit in Health and Disease PI: Mark Nelson, Ph.D. $11,447,202 P01 HL059408-11 Kathleen BrummelZiedins, Ph.D. Regional and Racial Differences in Hemostasis and Risk of Stroke and Heart Disease PI: Neil Zakai, M.D. $130,599 Cardiac Myosin Binding Protein-C: Molecular Mechanisms of Actomyosin Modulation PI: David Warshaw, Ph.D. $10,274,400 P20 GM103644-01A1 Vermont Center for Behavior and Health PI: Stephen Higgins, Ph.D. $1,533,382 N01 HC95166 Multiethnic Study of Atherosclerosis (MESA) II – Laboratory Center PI: Russell Tracy, Ph.D. $88,014 Matthew Watkins, M.D. P50 DAO36114 Neil Zakai, M.D. NIH – NICHD Tobacco Centers of Regulatory Science Local PI: Stephen Higgins, Ph.D. $2,924,426 PPG Project 1 National Longitudinal Study of Adolescent Health – Wave V Subcontract PI: Mary Cushman, M.D., M.Sc. $110,102 Molecular Mechanisms of ACTA2 Missense Mutations PI: Kathleen Trybus, Ph.D. $1,952,220 NIH – NIEHS PPG Project 2 P01 HL095488 Effects of Perfluoroaklyl Chemicals on Stroke Incidence and Mortality Local Subcontract PI: Mary Cushman, M.D., M.Sc. $6,746 14 car diovascu l ar r e search i nstitute of ve r mont Cav channels, TRP channels and vasomotor function in cerebral arterioles PI: Joseph Brayden, Ph.D. $1,362,000 • 2 0 14 a n n u a l r e p o r t PPG Project 3: P01 HL095488-01 R01 HL089944 Cerebrovascular Function during Ischemia and Reperfusions PI: Marilyn Cipolla, Ph.D. $1,900,000 Advanced Glycated End-Products in Human Myocardium PI: Martin M. LeWinter, M.D. $50,000 PPG Project 4 P01 HL095488 R01 NS073815 Impact of SAH on parenchymal arterioles and neurovascular coupling PI: George Wellman, Ph.D. $1,205,000 R01 HL071944-05 Pre-pregnancy Phenotype and Predisposition to Preeclamplsia PI: Ira Bernstein, M.D. $1,881,250 R01 NS045940-10 The Role of the Blood-brain Barrier in Seizure during Pregnancy and Preeclampsia PI: Marilyn Cipolla, Ph.D. $1,667,970 R01 HL059367 Epidemiology of Venous Thrombosis and Pulmonary Embolism Local PI: Mary Cushman, M.D., M.Sc. $205,334 R01 AG023629 Exceptional Survival: Trajectories to Function Subcontract PI: Mary Cushman, M.D., M.Sc. $36,230 R01 The Myofilament Basis of HFpEF PI: Martin M. LeWinter, M.D. $191,000 R01 HL121706-01A1 Regulation of Myoendothelial Function by Signaling Microdomains in Hypertension PI: Mark T. Nelson, Ph.D. $1,923,375 Control of reactive astrocytes by Notch1 and Amyloid Precursor Protein PI: Jeffrey Spees, Ph.D. $219,000 R01 AG033547 Muscle Disuse and Contractile Dysfunction in the Elderly PI: Michael Toth, Ph.D. $2,802,106 R01 Mutational Studies of Processive Myosin Motors PI: Kathleen Trybus, Ph.D. $1,747,810 Michael Toth, Ph.D. U01 NS41588 Etiology of Geographic and Racial Differences in Stroke Mortality Subcontract PI: Mary Cushman, M.D., M.Sc. $1,030,957 U10 HL110342 NHLBI Heart Failure Research Network – Vermont, New York and Quebec Regional Clinical Center PI: Martin M. LeWinter, M.D. $240,000 UM1 HL120877 Analysis and Characterization of Trauma-Induced Coagulopathy PI: Kenneth Mann, Ph.D., and Mark T. Nelson, Ph.D. (Lead Project 12 and Co-I Project 1) $23,769,600 www . uvm . edu / medicine / cvri 15 Research Funding: Highlights (continued) Non-Profit Funded Research American Heart Association Coupled myosin Va motor communication during cargo transport in vitro PI: M. Yusuf Ali, Ph.D. $308,000 Functional interaction between plateletderived growth factor receptor-beta and cyclic-AMPdependent protein kinase A PI: Paula Deming, Ph.D. $308,000 Pathogenesis of Small Vessel Disease of the Brain North American Coordinator: Mark T. Nelson, Ph.D. $6,000,000 (5 years) March of Dimes Foundation Mechanical Control of Chromosome Geometry PI: Jason Stumpff, Ph.D. $82,500 Preeclampsia Foundation Vision Award M. Yusuf Ali, Ph.D. Hydrogen peroxide and age-related sympathetic nervous system dysregulation PI: Benedek Erdos, Ph.D., M.D. $308,000 Mechanisms of EGF receptor activation leading to decreased cerebral blood flow after subarachnoid hemorrhage PI: Masayo Koide, Ph.D. $308,000 Hemodynamic Contributions to the Control of Neuronal Function PI: Thomas Longden, Ph.D. $47,000 Involvement of TRPM4 in pressure- and agonist-induced vasoconstriction in the cerebral microcirculation PI: Yao Li, B.S. $46,000 The Role of The Blood-Brain Barrier in Seizure During Pregnancy and Preeclampsia PI: Abbie Johnson, B.S. $46,000 16 Fondation Leducq car diovascu l ar r e search i nstitute of ve r mont The Role of Efflux Transporters on the Bloodbrain Barrier in Preventing Seizure During Pregnancy PI: Erica Hammer, M.D. $25,000 Totman Medical Research Trust Cerebrovascular Research PI: Mark T. Nelson, Ph.D. $150,000 Erica Hammer, M.D. Industry Sponsored Research Abbott Vascular ABSORB 3: A randomized trial comparing a fully bioresorbable drug eluting poly lactic acid polymer coronary stent versus a permanent everolimus eluting coronary stent PI: Harold Dauerman, M.D. $44,500 Baxter BioScience Evaluation of B-domainless Factor VIII Products PI: Saulius Butenas, Ph.D. $399,824 • 2 0 14 a n n u a l r e p o r t Saulius Butenas, Ph.D. Boston Scientific Prospective Randomized Evaluation of the WATCHMAN LAA Closure Device In Patients with Atrial Fibrillation Versus Long Term Warfarin Therapy (PREVAIL) and Continued Access to PREVAIL (CAP2) PI: Daniel Lustgarten, M.D. $66,000 diaDexus Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) Activity and the Risk of Stroke, Coronary Heart Disease and Cognitive decline in REGARDS PI: Mary Cushman, M.D., M.Sc. $50,340 Medtronic A Novel Treatment for Heart Failure with Preserved Ejection Fraction PI: Markus Meyer, M.D. $100,000 Attain Performa Quadripolar Lead Clinical Study PI: Daniel Lustgarten, M.D. $9,400 CoreValve® Expanded Use and Continued Access Transcatheter Aortic Valve Replacement Registries PI: Harold Dauerman, M.D. $873,500 for 92 patients CoreValve® Surgical Replacement and Transcatheter Aortic Valve Implantation (SURTAVI and Pivotal Randomized Trials) PI: Harold Dauerman, M.D. $273,400 for 26 patients Pacing-induced Remodeling in a Swine Model of Left Ventricular Hypertrophy II PI: Markus Meyer, M.D. $75,000 Pacing-induced Remodeling in a Swine Model of Left Ventricular Hypertrophy III PI: Markus Meyer, M.D. $20,000 Product Performance Platform PI: Robert Lobel , M.D. $23,000 SIMPLICITY HTN-3: A randomized trial of renal denervation versus maximal medical therapy for severe hypertension PI: Harold Dauerman, M.D. $100,000 Sanofi Odyssey Outcome Trial PI: Friederike Keating, M.D. $56,000 St. Jude Medical Quadripolar Pacing Post Approval Study PI: Daniel Lustgarten, M.D. $7,700 The Medicines Company Transition from Cangrelor to Ticagrelor, Prasugrel, and Clopidogrel PI: David J. Schneider, M.D. $136,800 Daniel Lustgarten, M.D. Markus Meyer, M.D. www . uvm . edu / medicine / cvri 17 Research Publications: A Sampling Across our academic medical center campus, throughout the region, and around the world, teams of physicians and scientists are dedicated to reducing the incidence, morbidity, and mortality of heart and vascular diseases through improving prevention, diagnosis and treatment. We are pleased to present a sampling of publications from our University of Vermont colleagues engaged across a wide range of cardiovascular research. Heart/Muscle Function and Failure, Heart Rhythm Disorders Angerer H, Radermacher M, Mańkowska M, Steger M, Zwicker K, Heide H, Wittig I, Brandt U, Zickermann V. The LYR protein subunit NB4M/NDUFA6 of mitochondrial complex I anchors an acyl carrier protein and is essential for catalytic activity. Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5207-12. Callahan DM, Bedrin NG, Subramanian M, Berking J, Ades PA, Toth MJ, Miller MS. Age-related structural alterations in human skeletal muscle fibers and mitochondria are associated with whole muscle size and single fiber function. J Appl Physiol 116: 1582-1592, 2014. Callahan DM, Miller MS, Sweeney AP, Tourville TW, Slauterbeck JR, Savage PD, Maugan DW, Ades PA, Beynnon BD, Toth MJ. Muscle disuse alters skeletal muscle contractile function at the molecular and cellular levels in older adult humans in a sex-specific manner. J Physiol. 2014; 592:4555-73. Hariharan, S., Kelm, R. J., Jr., and Strauch, A. R. (2014) The Purα/Purβ single-strand DNA-binding proteins attenuate smooth muscle actin gene transactivation in myofibroblasts. J. Cell. Physiol. 229, 1256-1271. Imazio M, Belli R, Beqaraj F, Giammaria M, Lestuzzi C, Hoit B, LeWinter M, Spodick DH, Adler Y; DROP Investigators. DRainage Or Pericardiocentesis alone for recurrent nonmalignant, nonbacterial pericardial effusions requiring intervention: rationale and design of the DROP trial, a randomized, open-label, multicenter study. J Cardiovasc Med (Hagerstown). 2014;15:510-4. Leary PJ, Jenny NS, Barr RG, Bluemke DA, Harhay MO, Heckbert SR, Kronmal RA, Lima JA, Mikacenic C, Tracy RP, Kawut SM. Pentraxin-3 and the right ventricle: The multi-ethnic study of atherosclerosis-right ventricle study. Pulmonary Circ 2014;4(2):250-259. LeWinter MM. Clinical practice. Acute pericarditis. N Engl J Med. 2014;371:2410-6. 18 LeWinter C, Bland JM, Crouch S, Cleland JG, Doherty P, LeWinter MM, Køber L, Hall AS, Gale CP. Impact of aspirin and statins on long-term survival in patients hospitalized with acute myocardial infarction complicated by heart failure: an analysis of 1706 patients. Eur J Heart Fail. 2014;16:95-102. LeWinter MM, Granzier HL Cardiac titin and heart disease. J Cardiovasc Pharmacol. 2014;63:207-12. Mauri L, Kereiakes DJ, Yeh RW, Driscoll-Schempp P, Cutlip DE, Steg PG, Normand SL, Braunwald E, Wiviott SD, Cohen DJ, Holmes DR Jr, Krucoff MW, Hermiller J, Dauerman HL, Simon DI, Kandzari DE, Garratt KN, Lee DP, Pow TK, Ver Lee P, Rinaldi MJ, Massaro JM, DAPT Study Investigators. Twelve or 30 months of dual antiplatelet therapy after drug-eluting stents. N Engl J Med. 2014; 371:2155-66. Meyer M, Bell SP, Sardana N, Zubarik R, LeWinter MM, Dauerman HL. Esophageal assessments of left ventricular filling pressures: A proof-of-concept study. Int J Crit Illn Inj Sci 2014; 4:18-23. Meyer M. Cardiac Atrophy. J Am Coll Cardiol. 2014; 64:38-40. Mohammed SF, Borlaug BA, McNulty S, Lewis GD, Lin G, Zakeri R, Semigran MJ, LeWinter M, Hernandez AF, Braunwald E, Redfield MM. Resting ventricular-vascular function and exercise capacity in heart failure with preserved ejection fraction: a RELAX trial ancillary study. Circ Heart Fail. 2014;7:580-9. Spector. P and Habel N. Principles of Differential Diagnostic Pacing Maneuvers: Serial vs. Parallel Conduction. Pacing Clin Electrophysiol. 2014 May 27. Subramanian M, Hunt AL, Petrucci GA, Chen Z, Hendley ED, Palmer BM. Differential metal content and gene expression in rat left ventricular hypertrophy due to hypertension and hyperactivity. J Trace Elem Med Biol. 2014 Jul;28(3):311-6. Swenson AM, Trivedi DV, Rauscher AA, Wang Y, Takagi Y, Palmer BM, Málnási-Csizmadia A, Debold EP, Yengo CM. Magnesium modulates actin binding and ADP release in myosin motors. J Biol Chem. 2014 Aug 22;289(34):23977-91. Tanner BCW, McKnabb M, Palmer BM, Toth MJ, Miller MS. Random myosin loss along thick-filaments increases myosin attachment time and the proportion of bound myosin heads to mitigate force decline in skeletal muscle. Arch Biochem Biophys 552-553: 117-127, 2014. Tanner BC, Wang Y, Robbins J, Palmer BM. Kinetics of cardiac myosin isoforms in mouse myocardium are affected differently by presence of myosin binding protein-C. J Muscle Res Cell Motil. 2014 Dec;35(56):267-78. Thompson NC, Stinnett-Donnelly J, Habel N, Benson B, Bates JHT, Sobel BE, Spector PS. Improved spatial resolution and electrogram wave direction independence with the use of an orthogonal electrode Configuration. J Clin Monit Comput (2014) 28:157-163. Mun JY, Previs MJ, Yu HY, Gulick J, Tobacman LS, Beck Previs S, Robbins J, Warshaw DM, Craig R. Myosinbinding protein C displaces tropomyosin to activate cardiac thin filaments and governs their speed by an independent mechanism. Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2170-5. Wang Y, Bishop NM, Taatjes DJ, Narisawa S, Millán JL, Palmer BM. Sex-dependent, zinc-induced dephosphorylation of phospholamban by tissuenonspecific alkaline phosphatase in the cardiac sarcomere. Am J Physiol Heart Circ Physiol. 2014 Sep 15;307(6):H933-8. Previs MJ, Michalek AJ, Warshaw DM. Molecular modulation of actomyosin function by cardiac myosinbinding protein C. Pflugers Arch. 2014 Mar;466(3): 439-44. Zakeri R, Borlaug BA, McNulty SE, Mohammed SF, Lewis GD, Semigran MJ, Deswal A, LeWinter M, Hernandez AF, Braunwald E, Redfield MM. Impact of atrial fibrillation on exercise capacity in heart failure with preserved ejection fraction: a RELAX trial ancillary study. Circ Heart Fail. 2014;7:123-30. car diovascu l ar r e search i nstitute of ve r mont • 2 0 14 a n n u a l r e p o r t Hemostasis/Thrombosis Ageno W, Di Minno MND, Ay C, Jang MJ, Hansen JB, Steffen LM, Vaya M, Rattazzi M, Pabinger I, Oh D, Di Minno G, Braekken AK, Cushman M, Bonet E, Pauletto P, Squizzato A, Dentali F. Association between the metabolic syndrome, its individual components, and unprovoked venous thromboembolism: results of a patient-level meta-analysis. Arterioscler, Thromb Vasc Biol 2014;34:2478-85. Aleshnick M, Orfeo T, Brummel-Ziedins K, Gissel M, Mann K. Interchangeability of rotational elastographic instruments and reagents. J Trauma Acute Care Surg. 2014;76:107-13. Alexander KS, Zakai NA, Gillett S, McLure LA, Wadley V, Unverzagt F, Cushman M. ABO blood type, factor VIII and incident cognitive impairment in the REGARDS cohort. Neurology 2014;30:1271-6. Belalcazar LM, Anderson AM, Lang W, Schwenke DC, Haffner SM, Yatsuya H, Rushing J, Vitolins MZ, Reeves R, Pi-Sunyer FX, Tracy RP, Ballantyne CM, Look ARG. Fiber intake and plasminogen activator inhibitor-1 in type 2 diabetes: Look ahead (action for health in diabetes) trial findings at baseline and year 1. JAcad Nut Dietetics. 2014;114:1800-1810 e1802. Bouchard BA, Gissel MT, Whelihan MF, Mann KG, Butenas S. Platelets do not express the oxidized or reduced forms of tissue factor. Biochim Biophys Acta. 2014;1840:1188-93 Brummel-Ziedins K.E. Developing individualized coagulation profiling of disease risk: thrombin generation dynamic models of the pro and anticoagulant balance Thromb.Research 2014; 133 Suppl 1:S9-S11. Brummel-Ziedins K.E., Everse SJ, Mann KG, Orfeo T. Modeling thrombin generation: plasma composition based approach. J Thromb Thrombolysis. 2014; 37(1):32-44. Brummel-Ziedins K.E. and Wolberg A.S. Global assays of hemostasis. Curr Opin Hematology 2014 21(5):395-403. Cushman M. Thrombophilia testing in women with venous thrombosis: the 4Ps approach. Clin Chem 2014;60:134-7. Cushman M, Callas PW, Allison MA, Criqui MH. Inflammation and peripheral venous disease: the San Diego Population Study. Thromb Haemost 2014; 112;566-72. Dauerman HL. Reconsidering the necessity of aspirin in stable coronary artery disease. J Am Coll Cardiol. 2014; 64:1437-40. Engbers MJ, Blom JW, Cushman M, Rosendaal FR, van Hylckama Vlieg A. The contribution of immobility risk factors to the incidence of venous thrombosis in an older population. J Thromb Haemost 2014;12:290-6. Folsom AR, Lutsey PL, Nambi V, Defilippi CR, Heckbert SR, Cushman M, Ballantyne CM. Troponin T, NT-pro BNP, and venous thromboembolism: The Longitudinal Investigation of Thromboembolism Etiology (LITE). Vasc Med 2014;19:33-41. Folsom AR, Lutsey PL, Roetker NS, Rosamond WD, Lazo M, Heckbert SR, Basu S, Cushman M, Selvin E. Elevated hepatic enzymes and incidence of venous thromboembolism: a prospective study. Ann Epidemiol 2014;24:817-21.e2. Folsom AR, Roetker NS, Rosamond WD, Heckbert SR, Basu S, Cushman M, Lutsey PL. Serum 25-hydroxyvitamin D and risk of venous thromboembolism: The Atherosclerosis Risk in Communities (ARIC) Study. J Thromb Haemost 2014;12:1455-60. Gebauer SK, Tracy RP, Baer DJ. Impact of stearic acid and oleic acid on hemostatic factors in the context of controlled diets consumed by healthy men. Eur J Clin Nutr. 2014;68:1072-1074. Hill AM, Stewart PW, Fung MK, Kris-Etherton PM, Ginsberg HN, Tracy RP, Pearson TA, Lefevre M, Reed RG, Elmer PJ, Holleran S, Ershow AG. Monthly hemostatic factor variability in women and men. Eur J Clin Invest. 2014; 44(3):309-318. Holmes CE, Bambace NM, Lewis P, Callas PW, Cushman M. Effectiveness of a short course of complex lyM.P.H.edema therapy or compression stocking therapy in the treatment of postthrombotic syndrome: a randomized controlled trial. Vasc Med 2014;19:42-8. Huang J, Huffman JE, Yamkauchi M, Trompet S, Cushman M, Strachan DP, O’Donnell CJ. Genome-wide association study for circulating tissue plasminogen activator levels and functional follow-up implicates endothelial STXBP5 and STX2. Arterioscler Thromb Vasc Biol 2014;34:1093-101. Kahn SR, Comerata A, Cushman M, Evans, NS, Ginsberg JS, Goldenberg NA, Prandoni P, Vedantham S, Walsh ME, Weitz JI. The posthrombotic syndrome: evidence-based prevention, diagnosis and treatment strategies. A Scientific Statement from the American Heart Association Councils on Peripheral Vascular Disease, Clinical Cardiology and Cardiovascular and Stroke Nursing. Circulation 2014;130:1636-61. Munir MS, Weng LC, Tang W, Basu S, Pankow JS, Matijevic N, Cushman M, Boerwinkle E, Folsom AR. Genetic markers associated with plasma protein C level in African-Americans: the Atherosclerosis Risk in Communities (ARIC) study. Genet Epidemiol 2014;38:709-13. Olson NC, Cushman M, Lutsey PL, McClure LA, Judd S, Tracy RP, Folsom AR, Zakai NA. Inflammation markers and incident venous thromboembolism: the REasons for Geographic and Racial Differences in Stroke (REGARDS) cohort. J Thromb Haemost 2014 12:1993-2001. Schneider DJ, Agarwal Z, Seecheran N, Keating FK, Gogo P. Pharmacodynamic effects during the transition between cangrelor and ticagrelor. JACC Cardiovasc Int, 2014;7:435-42. Schneider DJ, Seecheran N, Raza SS, Keating FK, Gogo P. Pharmacodynamic effects during the transition between cangrelor and prasugrel. Coron Artery Dis 2015;26:42-8. www . uvm . edu / medicine / cvri 19 Research Publications: A Sampling (continued) Sharma D, Brummel-Ziedins KE, Bouchard B, Holmes CE. Platelets in tumor progression: a host factor with multiple potential targets in the treatment of cancer. J Cellular Physiology 2014: 229:1005-15. Zakai NA, Judd SE, Alexander K, McClure LA, Kissela BM, Howard G, Cushman M. ABO blood type and stroke risk: the Reasons for Geographic Differences in Stroke (REGARDS) study. J Thromb Haemost 2014;12:564-70. Shea MK, Cushman M, Booth SL, Burke GL, Chen H, Kritchevsky SB. Associations between vitamin K status and hemostatic and inflammatory biomarkers in community-dwelling adults: the Multi-Ethnic Study of Atherosclerosis. Thromb Haemost.2014; 112 . Rehabilitation and Prevention Steinhubl SR, Eikelboom JW, Hylek EM, Dauerman HL, Smyth SS, Becker RC. Antiplatelet therapy in prevention of cardioand venous thromboembolic events. Journal of Thrombosis and Thrombolysis 2014; 37:367-372. Undas A, Brummel-Ziedins KE, Mann KG Anticoagulant effects of statins and their clinical implications.. Thromb Haemost. 2014, 111:392-400. Undas A, Brummel-Ziedins K, Mann KG. Why does aspirin decrease the risk of venous thromboembolism? On old and novel antithrombotic effects of acetyl salicylic acid. J Throm Haemost 2014, 12: 1776-87. Agarwal I, Glazer NL, Barasch E, Biggs ML, Djousse L, Fitzpatrick AL, Gottdiener JS, Ix JH, Kizer JR, Rimm EB, Siscovick DS, Tracy RP, Mukamal KJ. Fibrosis-related biomarkers and incident cardiovascular disease in older adults: The Cardiovascular Health Study. Circ Arrhythm Electrophysiol. 2014; 7:583-9. Agarwal I, Glazer NL, Barasch E, Biggs ML, Djousse L, Fitzpatrick AL, Gottdiener JS, Ix JH, Kizer JR, Rimm EB, Siscovick DS, Tracy RP, Zieman SJ, Mukamal KJ. Fibrosis-related biomarkers and risk of total and cause-specific mortality: The Cardiovascular Health Study. Am J Epidemiol. 2014; 179:1331-9. Weng LC, Tang W, Rich SR, Smith NL, Redline S, O’Donnell, CJ, Basu S Reiner AP, Delaney JA, Tracy RP, Palmer CD, Young T, Yang Q, Folsom AR, Cushman M. A genetic association study of D-dimer with 50K SNPs from a candidate gene chip in four ethnic groups. Thromb Res. 2014; 134: 462-7. Allison MA, Jenny NS, McClellan RL, Cushman M, Rifkin D. The associations of adipokines with selected markers of the renin-angiotensinogen-aldosterone system: the Multi-Ethnic Study of Atherosclerosis. J Hum Hypertens. 2014;29:127-33. Whelihan MF, Kiankhooy A, Brummel-Ziedins KE.Thrombin generation and fibrin clot formation under hypothermic conditions: an in vitro evaluation of tissue factor initiated whole blood coagulation. J Crit Care. 2014;29:24-30. Berkman A, Cole B, Ades PA, Dickey S, Higgins ST, Trentham-Dietz A, Sprague BL, Lakoski SG. Racial differences in breast cancer, cardiovascular disease, and all-cause mortality among women with ductal carcinoma in situ of the breast. Breast Cancer Res Treat. 2014; 148:407-13. Zakai NA, McClure LA, Judd SE, Safford MM, Folsom AR, Lutsey PL, Cushman M. Racial and regional differences in venous thromboembolism in the United States in three cohorts. Circulation 2014;129:1502-9. 20 Ades PA. A Controversial Step Forward: A Commentary on the 2013 ACC/AHA Guideline on the Treatment of Blood Cholesterol to Reduce Atherosclerotic Cardiovascular Risk in Adults. Coron Artery Dis. 2014 Feb 10. Brown TM, Voeks JH, Bittner V, Brenner DA, Cushman M, Goff DC, Glasser S, Muntner P, Tabereaux PB, Safford MM. Achievement of optimal medical therapy goals for US adults with coronary artery disease: results from the REasons for Geographic And Racial Differences in Stroke (REGARDS) study. J Am Coll Cardiol 2014;63:1626-33. car diovascu l ar r e search i nstitute of ve r mont • 2 0 14 a n n u a l r e p o r t Camacho A, Larsen B, McClelland RL, Morgan C, Criqui M, Cushman M, Allison MA. Association of subsyndromal and depressive symptoms with inflammatory markers among different ethnic groups: the Multi-Ethnic Study of Atherosclerosis. J Affect Disord 2014; 164:165-70. Cushman M, Judd SE, Howard VJ, Kissela B, Gutiérrez OM, Jenny NS, Ahmed A, Thacker EL, Zakai NA. N-terminal pro-B-type natriuretic peptide and stroke risk: the Reasons for Geographic and Racial Differences in Stroke cohort. Stroke 2014;45:1646-50. Ellis J, Lange EM, Li J, Dupuis J, Baumert J, Walston JD, Keating BJ, Durda P, Fox ER, Palmer CD, Meng YA, Young T, Farlow DN, Schnabel RB, Marzi CS, Larkin E, Martin LW, Bis JC, Auer P, Ramachandran VS, Gabriel SB, Willis MS, Pankow JS, Papanicolaou GJ, Rotter JI, Ballantyne CM, Gross M.D., Lettre G, Wilson JG, Peters U, Koenig W, Tracy RP, Redline S, Reiner AP, Benjamin EJ, Lange LA. Large Multiethnic Candidate Gene Study for C-Reactive Protein Levels: Identification of a Novel Association at CD36 in African Americans. Hum Gen. 2014; 133: 985-95. Fitzpatrick AL, Irizarry MC, Cushman M, Jenny NS, Chi GC, Koro C. Lipoprotein-associated phospholipase A2 and risk of dementia in the Cardiovascular Health Study. Atherosclerosis 2014;235:384-91. Gaalema DE, Higgins ST, Shepard DS, Suaya JA, Savage PD, Ades PA. State-by-state variations in cardiac rehabilitation participation are associated with educational attainment, income, and program availability. J Cardiopulm Rehabil Prev. 2014; 34:248-54. Garg PK, Biggs ML, Carnethon M, Ix JH, Criqui MH, Britton KA, Djousse L, SuttonTyrrell K, Newman AB, Cushman M, Mukumal KJ. Metabolic syndrome and risk of incident peripheral artery disease: the Cardiovascular Health Study. Hypertension. 2014; 63:413-19. Garin MC, Arnold AM, Lee JS, Tracy RP, Cappola AR. Subclinical hypothyroidism, weight change, and body composition in the elderly: The Cardiovascular Health Study. J Clin Endocrinol Metab. 2014; 371:22-31. Gillett SR, Boyle RH, Zakai NA, McClure LA, Jenny NS, Cushman M. Validating laboratory results in a national observational cohort study without field centers: the Reasons for Geographic and Racial Differences in Stroke Cohort. Clin Biochem 2014;47:243-6. Holmes MV, Lange LA, Palmer T, Lanktree MB, North KE, Almoguera B, Buxbaum S, Chandrupatla HR, Elbers CC, Guo Y, Hoogeveen RC, Li J, Li YR, Swerdlow DI, Cushman M, Price TS, Curtis SP, Fornage M, Hakonarson H, Patel SR, Redline S, Siscovick DS, Tsai MY, Wilson JG, van der Schouw YT, FitzGerald GA, Hingorani AD, Casas JP, de Bakker PIW, Rich SS, Schadt EE, Asselbergs FW, Reiner AP, Keating BJ. Causal effects of body mass index on cardiometabolic traits and events: a Mendelian randomization analysis. Am J Hum Genet 2014;94:198-208. Howard G, Wagenknecht LE, Kernan WN, Cushman M, Thacker EL, Judd SE, Howard VJ, Kissela BM. Racial differences in the association of insulin resistance with stroke risk: the REasons for Geographic And Racial Differences in Stroke (REGARDS) Study. Stroke 2014;45:2257-62. Karas MG, Benkeser D, Arnold AM, Bartz TM, Djousse L, Mukamal KJ, Ix JH, Zieman SJ, Siscovick DS, Tracy RP, Mantzoros CS, Gottdiener JS, Defilippi CR, Kizer JR. Relations of plasma total and high-molecular-weight adiponectin to new-onset heart failure in adults >/=65 years of age (from the cardiovascular health study). Am J Cardiol. 2014; 113:328-34. Kent ST, Cushman M, Howard G, Judd SE, Crosson WL, Al-HaM.D.an MZ, McClure LA. Sunlight exposure and cardiovascular risk factors in the REGARDS study: a cross-sectional split-sample analysis. BMC Neurol 2014;14:133. Keteyian SJ, Squires R, Ades PA, Thomas R. Incorporating patients with chronic heart failure into outpatient cardiac rehabilitation: practical recommendations for exercise and self-care counseling— a clinical review. J Cardiopulm Rehabil Prev. 2014; 34:223-32. Khawaja O, Maziarz M, Biggs ML, Longstreth WT, Jr., Ix JH, Kizer JR, Zieman S, Tracy RP, Mozaffarian D, Mukamal KJ, Siscovick DS, Djousse L. Plasma free fatty acids and risk of stroke in the Cardiovascular Health Study. Int J Stroke. 2014; 9:917-20. Kiage JN, Merrill PD, Judd SE, He K, Lipworth L, Cushman M, Howard VJ, Kabagambe EK. Transfat intake and incidence of stroke in the REasons for Geographic and Racial Differences in Stroke (REGARDS) Cohort. Am J Clin Nutr. 2014; 99: 1071-6. Kizer JR, Benkeser D, Arnold AM, Ix JH, Mukamal KJ, Djousse L, Tracy RP, Siscovick DS, Psaty BM, Zieman SJ. Advanced glycation/glycoxidation endproduct carboxymethyl-lysine and incidence of coronary heart disease and stroke in older adults. Atherosclerosis. 2014; 235:116-21. Kraja AT, Chasman DI, North KE, Reiner AP, Yanek LR, Kilpelainen TO, Smith JA, Dehghan A, Dupuis J, Johnson AD, Feitosa MF, Tekola-Ayele F, Chu AY, Nolte IM, Dastani Z, Morris A, Pendergrass SA, Sun YV, Ritchie M.D., Vaez A, Lin H, Ligthart S, Marullo L, Rohde R, Shao Y, Ziegler MA, Im HK, Cross Consortia Pleiotropy Group, Cohorts for Heart and Aging Research in Genetic Epidemiology, Genetic Investigation of Anthropometric Traits Consortium, Global Lipids Genetics Consortium, Meta-Analyses of Glucose, Insulin-related Traits Consortium, Global PBgen Consortium, ADIPOGen Consortium, Women’s Genome Health Study, Howard University Family Study, Schnabel RB, Jorgensen T, Jorgensen ME, Hansen T, Pedersen O, Stolk RP, Snieder H, Hofman A, Uitterlinden AG, Franco OH, Ikram MA, Richards JB, Rotimi C, Wilson JG, Lange L, Ganesh SK, Nalls M, Rasmussen-Torvik LJ, Pankow JS, Coresh J, Tang W, Linda Kao WH, Boerwinkle E, Morrison AC, Ridker PM, Becker DM, Rotter JI, Kardia SL, Loos RJ, Larson MG, Hsu YH, Province MA, Tracy R, Voight BF, Vaidya D, O’Donnell CJ, Benjamin EJ, Alizadeh BZ, Prokopenko I, Meigs JB, Borecki IB. Pleiotropic genes for metabolic syndrome and inflammation. Mol Genet Metab. 2014; 112:317-38. Lakoski SG, Mallick H, McClure LA, Safford M, Kissela B, Howard G, Cushman M. A risk algorithm for assessing short-term mortality for obese black and white men and women: the REasons for Geographic and Racial Differences in Stroke (REGARDS) cohort. Obesity (Sliver Spring) 2014; 22:1142-8. LeWinter C, Doherty P, Gale CP, Crouch S, Stirk L, Lewin RJ, LeWinter ML Ades PA, Køber L, Bland MM. Exercise-based cardiac rehabilitation in patients with heart failure: a meta-analysis of randomized controlled trials between 1999 and 2013. Eur J Prev Cardiol. 2014; Epub ahead of print. Logsdon BA, Dai JY, Auer PL, Johnsen JM, Ganesh SK, Smith NL, Wilson JG, Tracy RP, Lange LA, Jiao S, Rich SS, Lettre G, Carlson CS, Jackson RD, O’Donnell CJ, Wurfel MM, Nickerson DA, Tang H, Reiner AP, Kooperberg C. A variational bayes discrete mixture test for rare variant association. Genet Epidemiol. 2014; 38:21-30. Matteini AM, Li J, Lange EM, Tanaka T, Lange LA, Tracy RP, Wang Y, Biggs ML, Arking DE, Fallin M.D., Chakravarti A, Psaty BM, Bandinelli S, Ferrucci L, Reiner AP, Walston JD. Novel gene variants predict serum levels of the cytokines IL-18 and IL-1Ra in older adults. Cytok 2014; 65:10-6. McClure LA, Kleindorfer DO, Kissela BM, Cushman M, Soliman EZ, Howard G. Assessing the performance of the Framingham Stroke Risk Score in the Reasons for Geographic and Racial Differences in Stroke cohort. Stroke 2014;45:1716-20. Miedema M.D., Maziarz M, Biggs ML, Zieman SJ, Kizer JR, Ix JH, Mozaffarian D, Tracy RP, Psaty BM, Siscovick DS, Mukamal KJ, Djousse L. Plasma-free fatty acids, fatty acid-binding protein 4, and mortality in older adults (from the Cardiovascular Health Study). Am J Cardiol. 2014; 114:843-8. Muntner P, Colantonio L, Cushman M, Goff DC, Howard G, Howard VJ, Kissela B, Levitan EM, LloydJones DM, Safford MM. Validation of the Atherosclerotic Cardiovascular Disease Pooled Cohort Risk Equations. JAMA 2014;311:1406-15. Muntner P, Safford MM, Cushman M, Howard G. Comment on the reports of over-estimation of ASCVD risk using the 2013 AHA/ACC risk equation. Circulation 2014;129:266-7. Muse ED, Feldman DI, Blaha MJ, Dardari ZA, Blumenthal RS, Budoff MJ, Nasir K, Criqui MH, Cushman M, McClelland RL, Allison MA. The association of resistin with cardiovascular disease in the Multi-Ethnic Study of Atherosclerosis. Atherosclerosis. 2014; 239:101-8. www . uvm . edu / medicine / cvri 21 Research Publications: A Sampling (continued) Myocardial Infarction Genetics Consortium I. Inactivating mutations in npc1l1 and protection from coronary heart disease. NEJM. 2014; 371:2072-82. NHLBI Grand Opportunity Exome Sequencing Project. Whole-exome sequencing identifies rare and low-frequency coding variants associated with LDL cholesterol. Am J Hum Genet. 2014; 94:233-45. Odden MC, Shlipak MG, Whitson H, Katz R, Kearney PM, Defilippi C, Shastri S, Sarnak MJ, Siscovick DS, Cushman M, Psaty BM, Newman AB. Risk factors for cardiovascular disease across the spectrum of older age: the Cardiovascular Health Study. Atherosclerosis 2014;237:336-42. Ong KL, McClelland RL, Rye K, Cheung BMY, Post WS, Vaidya D, Criqui MH, Cushman M, Barter PJ, Allison MA. The relationship between insulin resistance and vascular calcification in coronary arteries, and the thoracic and abdominal aorta: The Multi-Ethnic Study of Atherosclerosis. Atherosclerosis 2014;236:257-62. Pack QR, Rodriguez-Escudero JP, Thomas RJ, Ades PA, West CP, Somers VK, Lopez-Jimenez F. The prognostic importance of weight loss in coronary artery disease: a systematic review and meta-analysis. Mayo Clin Proc. 2014; 89:1368-77. Rengo JL, Savage PD, Toth MJ, Ades PA. Statin therapy does not attenuate exercise training response in cardiac rehabilitation. J Am Coll Cardiol. 2014; 63:2050-1. Sanders JL, Ding V, Arnold AM, Kaplan RC, Cappola AR, Kizer JR, Boudreau RM, Cushman M, Newman AB. Do changes in circulating biomarkers track with each other and with functional changes in older adults? J Am Geriatr Soc. 2014; 69:174-81. Soliman EZ, Safford MM, Muntner P, Khodneva Y, Dawood FZ, Zakai NA, Thacker EL, Howard VJ, Howard G, Herrington DH, Cushman M. Atrial fibrillation and the risk of myocardial infarction. JAMA Intern Med 2014;174:107-14. Suzuki T, Voeks J, Zakai NA, Jenny NS, Brown TM, Safford MM, LeWinter M, Howard G, Cushman M. Metabolic syndrome, C-reactive protein and mortality in U.S. blacks and whites: the REasons for Geographic and Racial Differences in Stroke (REGARDS) study. Diabetes Care. 2014; 37: 2284-90. 22 Swerdlow DI, Preiss D, Kuchenbaecker KB, Holmes MV, Engmann JE, Shah T, Cushman M, … Keating BJ, Hingorani AD, Sattar N. HMG-coenzyme A reductase inhibition, type 2 diabetes and bodyweight: evidence from genetic analysis and randomized trials. Lancet 2014;385:351-61. Zemaitis P, Durazo R, Liu K, Jacobs D, Cushman M, Cooper R, Kramer H. Cumulative systolic blood pressure and changes in urine albumin/creatinine ratios in non-diabetic participants of the Multi-Ethnic Study of Atherosclerosis. Can J Am Soc Nephrol 2014;9:1922-9. Thacker EL, Gillett SR, Wadley VG, Unverzagt FW, Judd SE, McClure LA, Howard VJ, Cushman M. The American Heart Association’s Life’s Simple 7 and incident cognitive impairment: the Reasons for Geographic and Racial Differences in Stroke (REGARDS) study. J Am Heart Assoc 2014;3:e000635. Vascular Biology/Vascular Intervention/ Myocardial Infarction/Stroke Triglyceride and Hdl Working Group of the Exome Sequencing Project NHLBI. Loss-of-function mutations in apoc3, triglycerides, and coronary disease. N Engl J Med. 2014;371:22-31. Waterworth DM, Li L, Scott R, Warren L, Gillson C, Aponte J, Sarov-Blat L, Sprecher D, Dupuis J, Reiner A, Psaty BM, Tracy RP, Lin H, McPherson R, Chissoe S, Wareham N, Ehm MG. A low-frequency variant in mapk14 provides mechanistic evidence of a link with myeloperoxidase: A prognostic cardiovascular risk marker. J Am Heart Assoc. 2014; 3: e001074. Weiner SD, Ahmed HN, Jin Z, Cushman M, Herrington DH, Nelson JC, Di Tullio MR, Homma S. Systemic inflammation and brachial artery endothelial function in the Multi-Ethnic Study of Atherosclerosis. Heart 2014;100:862-6. Whitson HE, Arnold AM, Yee LM, Mukamal KJ, Kizer JR, Djousse L, Ix JH, Siscovick D, Tracy RP, Thielke SM, Hirsch C, Newman AB, Zieman S. Serum carboxymethyl-lysine, disability, and frailty in older persons: The Cardiovascular Health Study. J Gerontol A Biol Sci Med Sci. 2014; 69:710-6. Womack JA, Chang CC, So-Armah KA, Alcorn C, Baker JV, Brown ST, Budoff M, Butt AA, Gibert C, Goetz MB, Gottdiener J, Gottlieb S, Justice AC, Leaf D, McGinnis K, Rimland D, Rodriguez-Barradas MC, Sico J, Skanderson M, Tindle H, Tracy RP, Warner A, Freiberg MS. HIV infection and cardiovascular disease in women. J Am Heart Assoc. 2014; 3:e001035. car diovascu l ar r e search i nstitute of ve r mont • Ackerman S, Watkins MW, Kostial AF, Rabinowitz T. Urgent Assessment of Decision-Making Capacity in a Patient With Schizophrenia and an Evolving Myocardial Infarction Who is Refusing Care. J. Psychosomatics. 2014 Dec 12. Cipolla MJ, Chan SL, Sweet JG, Tavares MJ, Gokina N, Brayden JE. Postischemic reperfusion causes smooth muscle calcium sensitization and vasoconstriction of parenchymal arterioles. Stroke. 2014; 45:2425-30. Cipolla MJ, Sweet J, Chan SL, Tavares MJ, Gokina N, Brayden JE. Increased pressure-induced tone in rat parenchymal arterioles vs. middle cerebral arteries: role of ion channels and calcium sensitivity. J Appl Physiol, 2014; 117:53-9. Dauerman HL. Death and interventional cardiology: a tale of 2 trials. Circulation. 2014;130:1442-4. Dauerman HL. Deconstructing the language of bleeding. J Am Coll Cardiol. 2014; 63:1876-8. Dunn KM, Nelson MT. Neurovascular signaling in the brain and the pathological consequences of hypertension. Am J Physiol Heart Circ Physiol. 2014;306:H1-14. Gonzales AL, Yang Y, Sullivan MN, Sanders L, Dabertrand F, Hill-Eubanks DC, Nelson MT, Earley S. A PLCγ1-dependent, force-sensitive signaling network in the myogenic constriction of cerebral arteries. Sci Signal. 2014; 7:ra49. Hill-Eubanks DC, Gonzales AL, Sonkusare SK, Nelson MT. Vascular TRP channels: performing under pressure and going with the flow. Physiology (Bethesda). 2014; 29:343-60. Koide M, Syed AU, Braas KM, May V, Wellman GC (2014). Pituitary adenylate cyclase activating polypeptide (PACAP) dilates cerebellar arteries through activation of large-conductance Ca(2+)-activated (BK) and ATPsensitive (K ATP) K (+) channels. J Mol Neurosci. 2014 Nov;54:443-50. 2 0 14 a n n u a l r e p o r t Krishnamoorthy G, Sonkusare SK, Heppner TJ, Nelson MT. Opposing roles of smooth muscle BK channels and ryanodine receptors in the regulation of nerve-evoked constriction of mesenteric resistance arteries. Am J Physiol Heart Circ Physiol. 2014; 306:H981-8. Li Y, Baylie RL, Tavares MJ, Brayden JE (2014). TRPM4 channels couple purinergic receptor mechanoactivation and myogenic tone development in cerebral parenchymal arterioles. J Cereb Blood Flow Metab. 2014 Oct;34(10):1706-14. Longden TA, Dabertrand F, Hill-Eubanks DC, Hammack SE, Nelson MT. Stress-induced glucocorticoid signaling remodels neurovascular coupling through impairment of cerebrovascular inwardly rectifying K+ channel function. Proc Natl Acad Sci USA. 2014; 111:7462-7. Longden TA, Hammack SE, Nelson MT. Channeling Stress: inwardly-rectifying K+ channels in stress and disease. Channels (Austin). 2014; 8:296-7. Mercado J, Baylie R, Navedo MF, Yuan C, Scott JD, Nelson MT, Brayden JE, Santana LF. Nanoscale signaling between AKAP150, PKC, and TRPV4 channels in arterial smooth muscle. J Gen Physiol 2014; 143(5): 559-575. Mingin GC, Peterson A, Erickson CS, Nelson MT, Vizzard MA. Social stress induces changes in urinary bladder function, bladder NGF content and generalized bladder inflammation in mice. Am J Physiol Regul Integr Comp Physiol. 2014; 307:R893-900. Nausch B, Rode F, Jorgensen S, Nardi A, Korsgaard MP, Hougaard C, Brown WD, Bonev AD, Dyhring T, Strobaek D, Olesen SP, Christophersen P, Grunnet M, Nelson MT, Ronn LC. NS19504: a novel BK channel activator with relaxing effect on bladder smooth muscle spontaneous phasic contractions. J Pharmacol Exp Ther. 2014; 350:520-30. Sonkusare SK, Dalsgaard T, Bonev AD, HillEubanks DC, Kotlikoff MI, Scott JD, Santana LF, Nelson MT. AKAP150-dependent cooperative TRPV4 channel gating is central to endotheliumdependent vasodilation and is disrupted in hypertension. Sci Signal. 2014; 7:ra66. Taylor KA, Feig M, Brooks CL 3rd, Fagnant PM, Lowey S, Trybus KM. Role of the essential light chain in the activation of smooth muscle myosin by regulatory light chain phosphorylation. J Struct Biol. 2014 Mar;185(3):375-82. Johnson A, Chan S-L, Tremble S, Moseley J, LaMarca B, Nagle K, Cipolla MJ. Magnesium sulfate treatment reverses seizure susceptibility and decreases neuroinflammation in a rat model of severe preeclampsia. PLoS One. 2014; 9:e113670. Tian J, Dauerman H, Toma C, Samady H, Itoh T, Kuramitsu S, Domei T, Jia H, Vergallo R, Soeda T, Hu S, Minami Y, Lee H, Yu B, Jang IK. Prevalence and characteristics of TCFA and degree of coronary artery stenosis: an OCT, IVUS, and angiographic study. J Am Coll Cardiol. 2014; 64:672-80. Johnson A, Chan S-L, Tremble S, Moseley J, LaMarca B, Nagle K, Cipolla MJ (2014): Magnesium sulfate treatment reverses seizure susceptibility and decreases neuroinflammation in a rat model of severe preeclampsia. PLOS ONE 9(11):e113670. Tian J, Ren X, Uemura S, Dauerman H, Prasad A, Toma C, Jia H, Abtahian F, Vergallo R, Hu S, McNulty I, Lee H, Lee S, Yu B, Jang IK. Spatial heterogeneity of neoatherosclerosis and its relationship with neovascularization and adjacent plaque characteristics: optical coherence tomography study. Am Heart J. 2014; 167:884-92. van Diepen S, Reynolds HR, Stebbins A, Lopes RD, Džavík V, Ruzyllo W, Geppert A, Widimsky P, Ohman EM, Parrillo JE, Dauerman HL, Baran DA, Hochman JS, Alexander JH. Incidence and outcomes associated with early heart failure pharmacotherapy in patients with ongoing cardiogenic shock. Crit Care Med. 2014;42: 281-288. Villalba N, Sonkusare SK, Longden TA, Tran TL, Sackheim AM, Nelson MT, Wellman GC, Freeman K. Traumatic brain injury disrupts cerebrovascular tone through endothelial inducible nitric oxide synthase expression and nitric oxide gain of function, J Am Heart Assoc. 2014; 3:e001474. Vascular Function in Pregnancy: Preeclampsia Bernstein IM. The relationship of a family history for hypertension, myocardial infarction, or stroke with cardiovascular physiology in young women. Reprod Sci. 2014; 21:509-16. Buhimschi IA, Nayeri UA, Zhao G, Shook LL, Pensalfini A, Funai EF, Bernstein IM, Glabe CG, Buhimschi CS. Protein misfolding, congophilia, oligomerization, and defective amyloid processing in preeclampsia. Sci Transl Med. 2014; 6:245ra91. Mann SE, Maille N, Clas D, Osol G. Perivascular adipose tissue: a novel regulator of vascular tone in the rat pregnancy. Reprod Sci. 2014; pii:1933719114561556. McBride C, Hale SA, Subramanian M, Badger GJ, Schreurs M.P.H. and Cipolla MJ. Cerebrovascular dysfunction and blood-brain barrier permeability induced by oxidized LDL are prevented by apocynin and magnesium sulfate. J Cardiovasc Pharm. 2014; 63:33-9. Merhi Z, Doswell A and Cipolla MJ (2014): Vitamin D alters genes involved in follicular development and steroidogenesis in human cumulus granulosa cells. J Clin Endo & Metab 63(1):33-9. Osol G, Bernstein I. Preeclampsia and maternal cardiovascular disease: consequence or predisposition? J Vasc Res. 2014; 51:290-304. Osol G, Moore LG. Maternal uterine vascular remodeling during pregnancy. Microcirculation. 2014; 21:38-47. Sammar M, Nisamblatt S, Gonen R, Huppertz B, Gizuararson S, Osol G, Meiri H. The role of the carbohydrate recognition domain of placental protein 13 (PP13) in pregnancy evaluated with recombinant PP13 and the DelT221 PP13 variant. PLoS One. 2014; 9:e102832. Schreurs MPH and Cipolla MJ (2014): Cerebrovascular dysfunction and blood-brain barrier permeability induced by oxidized LDL are prevented by apocynin and magnesium sulfate. J Cardiovasc Pharm 63(1):33-39. Wallace K, Tremble SM, Owens MY, Morris R, Cipolla MJ (2014). Plasma from HELLP syndrome patients increases blood-brain barrier permeability. Repro Sci 22:278-284. www . uvm . edu / medicine / cvri 23 In Memory of Burton E. Sobel, M.D. University Distinguished Professor of Medicine E.L. Amidon Professor and Chair of Medicine Founding Director, Cardiovascular Research Institute of Vermont An inspiring and internationally-recognized leader in cardiovascular medicine, Dr. Sobel founded the Cardiovascular Research Institute of Vermont (CVRI) in 2002 and served as its Director until his death in 2013. To honor his memory and continue his legacy, the Burton E. Sobel, M.D. Memorial Fund was established at the University of Vermont. A fter graduating from Cornell University, Dr. Sobel obtained his M.D. from Harvard Medical School (magna cum laude) followed by a residency in Internal Medicine at Peter Bent Brigham Hospital and cardiology training at the National Institutes of Health. He began his career on the faculty at the University of California San Diego and then joined Washington University in St. Louis as Director of the Cardiovascular Disease Division. He subsequently rose to become Distinguished Professor in Cardiovascular Disease and Director of the Center for Cardiovascular Research. In 1994, Dr. Sobel relocated to the University of Vermont to lead the Department of Medicine, and was honored as an inaugural University Distinguished Professor in 2009. 24 He received many prestigious awards; led diverse research and training programs funded by the National Institutes of Health, the American Diabetes Association, and the American Heart Association; published more than 800 manuscripts; edited major cardiovascular and medical scientific journals; and held several patents. Dr. Sobel was a consummate scholar. Research he led was responsible for enzymatic sizing of myocardial infarction and subsequently thrombolytic therapy for the treatment of ST elevation myocardial infarction. During the past two decades Dr. Sobel focused his energy on diabetes and particularly the role of plasminogen activator inhibitor type-1 (PAI-1). During his career he was the editor or associate editor of top medical journals including Circulation, the car diovascu l ar r e search i nstitute of ve r mont • Journal of Clinical Investigation, American Journal of Physiology and Coronary Artery Disease and served on numerous scientific advisory committees for the NIH and other premier research organizations. He was also a highly regarded leader in subspecialty societies including the American College of Cardiology, the American Heart Association and the Society for Experimental Biology and Medicine. Dr. Sobel’s contributions were nationally recognized through numerous honors and awards including Distinguished Scientist of the American College of Cardiology, election to the Society of Clinical Investigation, and the 2010 Distinguished Scientist Award from the Society for Experimental Biology and Medicine, who also honored him with the named Burton E. Sobel Annual Young Investigator Award. 2 0 14 a n n u a l r e p o r t Ca rdiovascu l ar Re se arch I ns ti tute o f Ver m o nt U niv ersi ty of Ve r m on t C o l l ege o f M ed i ci ne Colcheste r Re se arch Faci l i ty 3 6 0 Sou th Par k D r i ve Colcheste r, Ve r m on t 0 5 4 4 6 8 02- 65 6 -8 9 5 5 w w w.uvm .e du / m e di ci n e/ c vr i