Eppendorf Research' . multi fix . variable

Eppendorf
Research'
.
. multi
fix variable
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ll
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ffi-t
g-.
e-a
g-t
€Fl
B+l
/a
l-J 'o
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lll-'
tl
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3
a
100- 10@pL
500- 50@!t
- 1O00
U!
i-'
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(!
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IT
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c ab n A / h A / P r b A
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Sedionungenl. iung
M o< r d. m pt oi . .
lnslfu2ro.idlmpiogo
M anu. ld€hs r r uc c ons s
P.da/ta
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B/&dot|!/b
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.......5
.....24
.35
.. 50
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65
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a/ P..t l
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M ar nls anc e . .
Ordeng Inlmano.
W anu6g. . . . .
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.
B6r e linlom alDnen.
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...103
Fg /Abb Resrch - Mainleanc6/ Wanuno
Fg. / Abb1 F66ar c h- v a . a b l e/ l i x . . . . . . . . . . . . . . .
......3
Fig7Abb. 7 Fiq. / Abb.
t 1 R . @ a h m u t t i c h a n . . /l M o h f i a n a r . . . . . . 1 1 9
eppendod and epp€.dorl Researchara @gi3lefedlEdemaac.
R€qEr€€d rnd.marG m nol marked n .llcas
wirh rM or o n rh s ms!.a|.
U.S Pal6t No 5.$r,131:..961,3so
No pan ol lhis publicalion may b6 Eprod@ed *ithoul th. prior psmi$io.
Copynqhlo 2OO7Epp..dorl AG, Eamblrs
ol lho
I
S.tuty Fldlilon.
rd .pCllc.doml llnlt !on. . , . . , . . . . , . . , . . -.6
Hddling
..............6
1 .2 Cm m dm inr s anc e.
..
..6
2
T. c t r . c . l ddr . . . . . , , . , , . , , . . , . . , . . . . . . . . . . . , . . , . , . . , . , , . , .
7
2 .1 R€$mh, fx€d-voluhe
..
.7
2.2 F':,!fuh, adrustable'voume (v.iabl6). . . . . . . . . . . . . . . .
7
(vanabl6)
2 .3 Fe*ft h, mlrt channer,.dlusrabr.-voruh€
4 .3
51
5 2 1 Whs should al€nmenl b€ @td our?
5.2.2 Follow- up
dqn' M l
lhe@ ol @r
................12
..
........13
'
n
5.2.3 MJuslmsl lor lqud3 wilh a densilyddlddl torhalolwal{
.....15
C€c / s i. r iliz lt id. . . .. . . , . . , . . , . . . . . .
-.. -....... -......... 15
61
Cae. . . . . . .
..
......
15
62
Sls lr at ' o. . .
......................16
6. 21 S ngec hanr elm ode t
..........16
6.2 2 M ulilc han. €lm odel.
...11
piof ro d spatch. . . . . . . . . .
6.3 O@onlaminallon
17
Tt ubl6s bor ins . . . . . . , . . , . , , . . , . . , , . , , . , . . . - . . . . . . . - . . . . . . 1 8
lr lhe@ s doubi$at d6pensin0daia areco@t
....19
I !.t'
D||Id.|r
r*'.Ftori
lrlr.to|I
Tho F6se4h p'petle rs a ab ddrce h may ooly bo opsated by atp.op@let
Boio€ wo&inq wirh rh6 R6€rch p pefie, pleaso €3d rhe op&atrg manual.To
6su6 ste poblem'lre expdmenb wnh rhe R6@ah ap6lte. il rs 6strd
to ob34e lho tollowng poi.ns:
- Only u* p'pen6 n lhe Fes€eh Fam ly whm a p,petle tip has be6n
- Prp€teiips areelery d6iqn6d lor d6po$bl6 !sge.
u36 rhe 1- 10 mL pip6ite ory wth fhe
'ns6rod
- oo not lay downth6 p penewh6na lil6d pipetlolrp s anached.
whs usng nletous. radr@ive. lonc and/d $lul ons lhal posa r h@nh
.sk. pkre ot646lh6 eiety D@uions lalrd lor rhe counrry ln wnich tne
whe ueng dganic aolv6ls and agglaive chfi'cals, .heck rhe suirabilty
ol u* wnh p'pett6lrps (madeol PP = polypmpyl*)and lha pDen6
wh.. !s ng $ ul oB wth phFrca chaEclen$cs whichaE consdedby
dilldentlo thoe of watq (6.9 glycebl),chek the dispsnsingvolum€as
d*nb€d rn s€.I on 5 2
- Avo d difie€nces In lempdalvE b€tw€€n pip€nes and prp.n€ trps as well a3
rhe iquid lsed as th 5 may l6ad lo inco|Et volum6s bqng d'spens6d.
- The abov6 nayd$ 6cur whs liqud3 wth a h'gh vapor p'su€ @ used
1.2 C.E and hrinl.nance
- Do nor dlow any lqud lo drs nlo ths prpetie
- Do rci . 6anlhe prp.new rh acdonoor agqesew solul'ons
- U* onginal3pm padsand accso. .s (p|p€tloI ps)o.ly.
c
2.1 R€grch,liEd-Yolure
@
2.2 R.s.arch,adjustabr€-vorum.(v.ri.br6)
f
i:ia
i
s
.i-
r"
2,3 R.s..ch,
tRr
|a4
mutb-ch.nn l. *liGltbL-vorure
cdqcd-
trria
wm
q
{v.ri.bl.)
.'.rEEf,u
' i6
''.
.'"'
Thelehnrca dala is vald onlywhenlhe quol€dEpp€ndorlpipe& npsaF used
Tsis cad6d od n accodancewithISO8655lor pston_slokeppeli6s w th an
a r cush'on us ng a fine balance wnh mo'slue tap apprcved bv lhe
standardzarDn adhontrG
Nole:on the 1-10 mL p'pene.whenrh6I - 10 mL lrp long(se orde.ng
mlomal'oi) s used lh€ quoledloleranceranqencEas€sbv a lacloro, 2
comparcdro whenlh€slandadlp s !s.d
Numbd ol delm nanons:
10: d€gass.d. b'disii0€d wald,
20"C 25'C conslanilo : 0 s 'C: wlh p@ weil€dppsnonp:
dsp€nsingcanledoul on nnerwalol v6$61
Ilthe placewh6relhe p pelle lsed s at €xl€me v h th alliludo.ai adlushem
's
must b€ mad€ n llne wlh lhe amb enl a I pEssure
Thepp€fi6 b€long'nglolheReseaohFamrlva/e pslon stoke p p6lleslhal
operateaccodrg to lhe 5tr'cushonpnncple
!tu
TheR*aEh Familyco.s sls ol ixed'volumepipetlos.adluslable_vo
{vdable) p'pen6 and dghl and twelv€-channelp p3n6 w
(vanabre)
*nrns.
adtuslabr6.volum€
eparale I p eJdnon
al mod6l! belonginglolheBe$dn Fam'Lyhave
pislon.whch m6anslhal s
1s
ow.
mult
Eachchannelollhe
-chann€lmod6lhas
posibr6 ro anachfewerlhan eEhl oriwervel|ps
In adddon.lhe owetpan canbe oialed nlo a usd_del'nd pos 'o. as Equ'd
I
h is possiblo to cho@ b6te@ a lowd pan br oiOhtchamls and ld lw6lve
chan..6, Borh cow the see volome Enge.
Ftg.2 Rdarch ({ringle{hereI) and muhi-ch.nn6l low
2
pan
FirEi$top (@nng
strck€)i ths
sspnabd 6lum. is dispdFodSedd stop (bl@-d4; th.liquid
Maining in tho tip is blown dn.
2 S€ning.ing
@
Fq firsd-voluhe pipott€s, this nng is
ror adjuslrMt puDo* only.
whh ovorlappingcslibEhon 3.al
ls in*ting ths wt€rch dunng
To sxtracl liquids nom |qq vgsls, fl€
et€ction dse roy bo polled ofr whs
rh€.j&1io blnon is h€ld d n.
7
6 Multi-chan.el lorer pan whh:
a
a Op.ning ld ths t@l lor digaasdbly
4.1 \rohru eniry hot v.lld lq fircd-volum. nodel)
Thevoum€ca. b6changedco.r.uously by rclannglhesenrngnng
(Fg. 2-2).ThelEur6 n th6 d gla d spray@ ead tromrop ro bonom.
lr E advreb e ro carry od voiume son ng fton th6 h'ghd down lo lhe lowd
varue e nd go aboveth6 des@dvo umeand the. @r!m ro 1116
ow$ vaue
'
th€ rlFtt
od tumd6
only *h.n a plp.tte np i. rtt chcd i.to Ehich rh.
io lac llare lhe *arch tor a sunab6r|p,fie colorol rhe contd bunds
coGpoods lo lhe color ol ihe Epp€ndorl I p r3cks.
wh€. p p.nrng lqu ds w h welt ng pop€rl6s d fie@ntto rhoseol wal6l pl6ae
ob*toe lho Gcommendalonsconlared $cton a 5
'n
- Th€lqu'dwh'ch Flo b6 aspral6d slake.lDm a sunabbves*l1lo. m!hiwe Eommen! lhe @genr lervor 'Tp.Tub-).
chann6rpipen6s.
- atrachslirableI'ps rhe D'p€tlelimry lobede lhe colorcod ng)
- 1- 10 mL mode. u* pipen6onlywlh llter rnserr6d
- Pressdownth6 conlolburion (F'9.2-1)toln6nd dop (measufngsroke)
- rmneGerhe p'pen6tp{s) apDrcx.3 nm nrorherqurd.
For R6@rch. ad,usiablevorume(vanabe).500-5OOO
uL and I -10 mL:
- Arow lhe contol buttonto srd€ back3lowly
pLand
voume (vanable).
50o-5OOO
- 8.106@movrngF6€an adrustabr6
1-l0mLiomrher'q!id. wa'l appEr malelyrh@ sonds
- Plr rherip(s)olr olrherq!'dslowt
- To@moveanyrema,nnq dropets.dabwih non-lib@lsc6lu ose Wh€ndo |l
so ensu@rharno
comesolt ot rher p(s)
'quid
t0
ForR@rch, adjust.blFvolum6(v4jable).500-5 000pL and 1 l0mtjilth€
lrp 6 Moved lM th6 lrquidtm quickly,c@i{t io|6 may pGn lquid out of
lhe tip. This may auh i. lh€ pip€tt€d voluhe b€ing i@ low
- Hold lhe lipls) al d anqle agsinst ths insid€ wall ol the tub€r'wellol ttE
olbuno. (fig,2-1) slowlytothe liFt stop (m6!nng
- Plsdownth€o
stE*6) and wail u.til th€ liquid srops nowing.
Pl$ down th6 @ntDl bufion ro rhe so.d stoke (blow od) untiltlE tip(s)
ivaE @mprotery6mpt.
Hold down th6 @nlol bunon and pull lhe lipls) up lho innd wall or th. lub€.
- Allow rh6 @rol
Tips @ q*l€d
(Fis.2-3).
bunon ro slide b&k d@t.
by pBslng tho .1&tion button on the side ot the pip€fie
Oo dt r.y do,n dE pip.n ,ts
a filLd dp.n
rnb m, clh In lhuid dtrins ItE pipgtt !
!p l. .tr..t'.d
..
To guadle pEisio and a@uacy, ws €lMmdd
pre-w€tting all nq tips by
aspiEting and dbp6si.g liqoid lso or lh@ tim6 b€lo€ pip.tting.
with th. rip nor in conret with th€ lhlid, 6mpty d compl€tat on rh6 innd qtt of
E4l.natior: why d6 lhe pipene ip havs to bs p6-stt
d?
To cohpsnels ld th€ p@FEni6 ol the liqoid.
V'teninghquids(sem, dstdgont) io6 a thln flm on the inner wall ol the prpotts
lip. when the n6t prpotting is @ni€d od, the volume disp€nsedwolld thus b€
When pipelting *rum o. hioh-vi$6it
&praling sd disposing I quid.
soldions, wait a lew s@nds wh6
! t ba/.|lr.rn
The ser a] .umb€r s o. lhe s6tr ng nng on the co.rdl bunon
Fe$arch, sin9r.-chann.l
O'stiledwalr is dispons6diofr a pB wen€dr p nlo a rLbeand 6lhs
Th6resr s p€rlomedw rh a highlys6ns Ne analyncba ance(@solurDn
0.001ng) by el6as'n9lho vo um6
Irra bldinilb<l sts, sighing
b€ th€ dm i.mp€EtuFl
v6el,
pap.tte .nd plp.tt
tip m!.i
lo calcuala lhe vorlme.d v'deih6 weqht by lhe denstyor the wat&
(ai20 "C:0.9932mgfuLrmg,?!L= g/mL)
Vdums > lO !l- lO m!
Fora vollme ol moB rhan10 !L a balancewth a 6e ut on ol0 01 ng Ls
B.e.rch, Dlld{hann.l
Mun-channelp'p6tlesaE lsua ly lesledby drsp€nsnq the volumolom a p.e I
51.1 Wh€n should rlignment be canied oul?
Thep,peh* belongrnglolhe F6searchFamrywe6 t6st6dwth walerdunng
podlct'on n a.codance wih ISO8655and p@videdw n a car'brat'on
sa t
In lh€ c6e ol doublsansinqwlh Bgad lo lhe accuracyollhe o'pen6dvolum
lh6tolowng ponls shouldbo clanned
lsihe p'pen6 6akrs? llhrs
@asonior dFpensedvoures
be'hqloo ow:ioubr6h6n.g'sonepossbe
and elnons a€ conlaLnod
. sa. 7 )
12
- Whal is lh€ lmpdaru€ ol th€ sed€? (ln ops tub6, wat* at @m
lmp€ctuE €vapoat6)
ot rhe pipotte?
- whar is rhe rdp-aru€
ot th6 aia
- whal is lh€ lsp€tlE
- H6 mg ben coven€d inlo !L?
OG th€ smd6 havEa diftrnt dssitv to that ol qt6/l
ls lho Pipsttng sp€€d t@ high?
lr tha pl&o whw the pipene is us€d is at exlrmely high ahilud€, d adjuslmdl
muet b€ msd. in liE with f1e mbisl ai. p|6uB. At 1,000 m above s level,
th* b a voluhs .mr ol emx. -O.4 %.
Asist n@ wilh th@ qu6tioB is @nlai.€d i. Eppenddf! SOP (S_tandard
OpsEting Pc€duE), which is availableupon €qu6t. An SOP 6n be @lled op
noh @r homs pege Mepp€rddlcom.
I l'|.$ ch.cr! D.!v. lo b. orl|lc.dtu|.
il l. ot to .9m
lt t rh.
dCnmd or t E pi-tt
h.. rn Gd (..q. dF lo t.{dl
@mpomd. h.n
'g
522 Follow-updbffit
ln lh. @$ ol .frr
Fm a lehni.al pornl ol vi*, thrs is a zm-point shift. Ihs val!. by whbh th€
s.ning ol the piFne is shttted llmins co6tanl acds th€ sniB m6unng
.ang.. lr, td .xodq
in lh€ c@ of a 10-100 pL, toll4 up alignm4l ot
1 !L tg*6 dee.r 100!r (=1%),the pip.tte is d$ adjlst.d by 1 !L ai 10 !l
(=10%t
a Th6 pipotts, tip ard watd nusl .ll b. rh€ she temp.EluB
(2G25 'C. cond4n ro 10.5.Ct.
b In tho c4 oi adjusiabls pip€tt6, th€ pip.tte is st to the nominalvolume
With a p|potl6 tip atlach€d to iho pip€ne, rhe d6iEd volufre is pip€tt€d
and weighed 10 tim6 (lor muhi chdndpip€n6, lhi. i5 wi6d out lor *h
chann.l) Ihe mqn ol lhis soighino is @nvdl€d into !L usinoth. lollwing
(arthe lempqaluro sp€ifi€d)
The value oblained is tho actual s€ttinq
ldssily ot wa14al20'C: 0.9s32ng/pl).
d
6
To align io lhs volume di3played,sido O (or 3 de B whh mulli-channe
p p€fi6) ol th6 wnch is hs.n€d hdEonlally lhDvgh lhe c€lb.arbn s
nrohe arionm.ntop€nng(FE.2-4)r ft€ pDd6 gnp 0n/hendoinoso
hold tho prpen6 venrelly.)ft€ w€nch is ths orared nro a venrcal
posilDn The cal|brat'on seal s thseby desldyed and cfrov€d.
(v.ri.bL) and mo|li{n'|*l
i.!e$,
ad,!.trbL-vollm
wh6n th6 eltinq r nq is olated (e ho. in lh6 + or , direlion),rh€ piston
sloke ol lhe pipetto 6 alto€d (athough the volume *ning drar6ans
om ElaiDn B equarto lh6 tollowng:
F.@rch, adhrrabb.vollm
ft.n.bb)
0.1 - 2.5 !L atpor. 0.1 pL
0.5 10 !L appox 0.5 !L
2 20 !L aopor
1 pL
10 - l0O !L appbx
5 !L
2m !L appDx. lopL
20
100 1000 !L aPpor 50trL
500 50m !L appDx 250pL
1 10 m L a p p o x 5 1 0 p L
F6$.6h, hu|d-cn nn l
0.5 - l0 !L approt. 05
10 - 100 !L approx. 5
30 - 300 !L approx 1C
Rasnh,
fuod-volsm
lo €duc€ tho volun.,luh rh€ s.ning nng (Fig 2'2).lockw6e.To nc
ih6 volutu, turn the ettng.ng cdnrsbckwre
one rctatDn6 eq!.t to th6 totowno
I
I
R6mov6lhe w@ch and oove th€ sEn ng i.O backwardsand loaads
u.n lh6 cou.ler and lhe 3lok. sysl4 l€k logorh*
R6peal siep c) The r€dings mlsi b6 wth'. the rol€ranc€ssp&ilied r n{
ls lha nominalvolumed6
la
nol c@spond wiih lhe m.6unng 6!lt. 6per
sh@ ihb diunnsn afock iho dri6 m.ei.g Eng, ir is imp.ratM ro cnek
iha ot ra. volurc ol thb 9iFli. sperf€d in lh. [email protected].
Naxi,d@ th. djGltrst opaningwitho.1€ol lh. &ppli€d dllratb. sdl3,
5.2.1 ACqtmd to. llqdd snh . d.dty dlli..r.rt to |tst or qbr
t E pos.ibloto adt6l m .t .aic volurr. or liriii witha (bltry ditbqn to
ItEl ol w.t r in s6h a say llsl iha volun€dbFlay.<ldEpo.d3 ronE vdlm
Al o0r rir..
.4'd.!|.-t.lrr
lo. tE .di6a.bL pb.tL. D |w dn ol .lil.rt.q
h. t
('deld pb.ri. h- b...r cadr.d arto. tu d-
Prcc..d s d€nb€d ih eteF a)lo g) ol S€c1io5.2.?.
A pipott€ st in ihis way ddisB . pip€ni.g v.luo thal @mlat6 slth lh.l
h th. display ont ld tip lhold u..<l .nd tor $. wlum rrt dl For
this @en, w3 reomm.nd Lboling th6 c@vd€d p'pone 6 a nr.<lvoltlmpiFtt
ld "sorutrony'|
tlE .@ rd liquijs with a hiohd vapor p|Err!
b. alN|n€din rhis way. In lhB c&. m .Edrs'd
6.!. oQEni: son€n6)ca mr
urng o Eppdddl pcittu -
f,ttr.ffit
d in€ tuq6c1 ot @, all plp.tl6 lhoold bo d€n€d in a s.6r'
Ming
solulbn o. shdld be csrtult wiFd dM Gi.g l.opEpaml They stDurd th.n
b. dn! d m distrrrodstd .nd dd.d,
Ih. $ds @ malnl4an*ft..
and lh€ pisloB Bhdld bo lubricated liohtly
(usi.g lh. silicone gEe p.ovld.d)wh6n cl6an6d or Eplaced.
Wilh lh. multi chdnol modsl, tho vl3ibl6 O-nn$ on iho lip holda shoold b.
Ighny |ubri€r6d and d@inq and ih.n slp€d winq a rinrl?e croih. l)o'€clivo
G.ing3 m6l b€ €pleed (s P'l a, 'Mainl.n.r1c.').
S€v€[email protected] (ar€d by lh€ liquld dlding liE pipono 6 b. Mov.d
lnt fi. pip.tt€ ha ben dila.smbl€d (s Pdl B. 'Maidlelra@').
In ords ro dsu€ lh6 cons€tsi qual'ty ol lh€ p'pett6. m
c'ne
'@mmdd
coGronding patu ol lhe em. q@ario.
(Physloca6Concopl+ Physioca@
Conc6pt,Slodad + Standad').
' de@ 6vrd old opdallng iNltucli@s
Vl@l dit cnfElinc
t .r!H
ol PhFioC.E
Conc.pl
N ":*,:"
nwfffl
The low hartol rh6 p'pene an be stFlized by m@s ol [email protected]
(121"C, r br,20 mhur6).
ll neesery allow the aul@lav6d parts to dry at oom lempe.atuc. Do nol
6smbl€ lhe pip€tt6 unlil il has c@led down comp €l6ly.
4.2.1 SingL-.rrenn l mod.l
wih srnglo-chmn€l
modelslh6 ete.l on sl&ve (Fq. 1 1?)3ndth6 lowsrpd
{F'9.1-10)may be auielaved
To do e, hord down lh6 eterion butl6 and pur ot lh6 or*tDn sr6ve, thF
uo$w rh€ pipefio low* pan (q{h pipend up ro I000 [. rh s hay equ]E
a$istancaol op€nmgA ol lh€ wBnch povded ss F 9. 3)
s|nsl+{rr.nnc,
dju.i.bla
wlqD
{vrilbLl
The p6lon husl b€ @moved b.io€ lhe rD.te loes
oad can b€ unscrcwod (w Part B, Mant6mrc€').
tt
v..trrt 1- lo mL (Frg.i-i4)
Pull frn* down dn ol lh6 n@ c@. Tha liltd swoll6 up slighlly dunng .ute
qsnt wlb llrlgiing- This d06 @r inp.ir tuEion. chry
chvir|o. cop|B
aut@|tu fltd 1x. ue6w
th. 6mprer. ro*8 pen fin fi. gnp by tuming ro
th.l.{r, nol rominorh.ojetio d6vs (Fig.1 12)et rh. sd. rlm. Th.3i&tio.
sLov6 ffi
od ot i|3 b.acl6t .utdEri.l|t
dunno tuming . d @ b. |!trb.<t
a3l Mut!-cll.dEl nrod.l
Wih itE mohi-channolrE<,el. lh. trd.i.
brd
pe,i (f:rs. 2€) is p|8c.d into
to do $, hold down ih6 d@rid button and uBFw the ldd pan ttom rhe Onp
by loiali.'g n c.tnnqdockwi$.
Al Fp€tt6 b€bnging ro the R€.dch FmiV my b.6xpo..d to W righi.
Any disroralion $hich may @cur hd no .ttect on lh6 tuncllon of lh6 plpott6.
a3 ot'lr
i.rbi
Ddo. ro dl'.rdl
n rh. i.dr.n
dFtr. b to b. cnckd, nt iEd or olibrt d by
Cped.brl AOo. mil.r .ddc. p-tFr, ll drt b. lr- or
lr.4&c.rtd-d-dc|.rl
a lom call.d 'o@r minatid crtncara ior Etum ol goods' 4d g6n6ril
rc16 abqi d@otamrnation @ avalebla on dr hom. p€dai
A ,gt€d dedrrmiEtion
dtifc€te m6t bc fttG.d
with th. pip€tto wh6 it
k |lllmsd The *id nlhbs ol lh. B6!€arch mu3t b€ entoEd @ th€
d@ntamiml$
cdtitcats.
Th. bot6 p.n ol ttF R6@rch 6 b. d-dtdridt.d
ot 9oranridv inlocti@!
.ub6r.|€
bt being 3rom-aurodaved ($ s4- 6.2).
Th. 8u.r&.. ol tho pipotl6 @n b€ disjniocl.d enh 6l@hol l6rhanot,prcp.ml) d
wilh a ditnl6ci.nr @naining akottol.
rroFabcl|n.
lrrnloath.
ph..i.S.
ftdr.a
d$tt|grdro.
!r v6lma
* pb.d.
- t r.rir $nhg ol tr
Dblcs.ll.
- ,{t dln
- A pb.rr. !p rt$ p...
s.ll|rr9F!Frt-hab...r
- r.r..'drgha
da
- th.!9 bb6.
- A poo.,tyitt{ pbdrc ip
ts b...r dd.
- Uqrrdlitr ! hilh v?o.
p.{r!
rE bdi pbib4
F AIF5lm y'Jl -10 nL
- ftlatrtpclfir
r
s.
- fudl9s-looh.
- n bE'rnoidtoo
qi.$y n!|n llr. rq|S.
- tl.' tlod. I dtpllo
t|btdr b dt
llq/id,
niid.d.
- F.olstn. pito.rrd
{PIr B,
*drrh.5(D-5@4
- n
ltd
b.i'F-ta
Fo. ndttd.nl
ho.Ll:
- fti Glig ot th. chrful
b
lPd8.
Thepston sconlam'naied
Theseal s contaminat€i
Ceanlhepsro. and
ubncateqhtly lPad B.
'Marnte.anc€)
- Femove the p slo. lom the
p petrerowerpad (PanB
'Manlenance")Bnse out
the owerpan wth warm
d sl red wals and a low io
dry Replacelhesean
- Removethe p'stontom lhe
L q u d h a s e.1e@dl he nose
Ma nlenance) andaerare
the lowefpan. C eanand
lighllylubrcal6th€ p slon
Femoveth6 pston lrcm lhe
pipefle owerpan (PalrB.
'Manrenance). Frnseo!1
the rowerpan wth wam
d6n bd walefand alow 10
It the€ is doubt th.r dbpensjngdata a€ comct
Toavod dspensrnQ
enors.the precs on and acc!6cy ol th€ Reseaahneedto
be ch€ked regua y TheP CASO sonwareprcqram(seeordefnCLnformation)
is avaiab e 1odelermne permrn€dsyslemalcand randommeasling devat on
(se Seclon 2 Techncadata ) An SOP(Standard
OperalnqProc€n!6)ror
crr€cknq p penescan be cal ed up iton alt h.ne pa9ewwveppendod.can.
For quds whosephyeca dala deval6 signiticanlly
ton tfroseotwaier
carb6ton needsto be chanqedn accodancew th setDn 5 2 3.
Ca rbranonw I alsoneedto be changedflhe p p€ne s be n9 lsed at a veryh,gh
19
L
Si. gle- c hanr cnl o d . r - . .
A D sa$emblya.d asssmby
A Beplacng pistonsandsears
ll. M uhi- c hanc lm o d e l, . , ,
A Repacmgthe O-nngs
I Feplacrng
lhe pislonseas
....-.....-.82
.A2
...42
....-.,.,,.,06
86
86
Pl..$ us only rhe.cc.t.od!r
rcommcnded by Epp.ndorL
u3ing spaE p.rt!.nd .omumrbl.. rhal we have nol rcomm.nd.d
rnay E<lud lh. Flcirdon, .ccuEcy .trd lii. ol lhe d.vic.t, !N. do not
hdo. a.y ,aftnv
d .cc.pl a.y Espomibility tor d.m.gc Blltihg
G@r:nt66 a.d s.mnry
In tho caseol wa(antycla'mspleasecontaciyour€spons'be Eppendon
W6a.and-learpa.tsarc excludedlomlh6sananry When@t!rnng lhe p pen6
lo lh6 Eppendort
contractualpannef
rhe ndessarydataloruti'zationand lhe
pe.romanceor deonlah nal on (s.e s6clon 6.3)shourdbe nc ude!
Sotu'ongmusl becanedour by the S€ruc6 ol the Eppendorlconrracrual
No wananlyshalapp y w th fr suseor op€nng ol the devce by unaulhorzed
ie
;r rnlomalon on €placrngpsions and sealsas wellas
emb rg lhedile€nl membercol the Research
tamly
3i.|9le-channel pip.ltes
I. manlenancework ca( ed oul usrngth s wGnch.
's
lpenngA:
;de B:
;de C
;d€ D:
lo
lo
lo
lo
loose.lhe p pene owerpanlomlhe Onp.
repiacethe seals
repiacethe seals
algn th6 pipene.
\ | marnlenanc€
wort( scamed out usinglhisloo
Sde A: to repacelhe O f.qs on the.ose cones.
sde B: to@movetheowerholsnq. to ms6n th€ m€ta1.ips 10€movelh6
llre prpette
Pcss P eceand lo a
SdeC: ior epac elhes eal s'gn
81
(OL)ledFnqeDsdd arua a'I6u.eFo
/ ! 6'l s)
AqrF6up\1.sr rd.qt
'ouad'd.ql otqoN*to
rrl OZor d. euedrd (qqere^) .unp^3nelsnlt"
.t s pu..@Ftd
-
pue -p3xu rol
?tupqd.u g
'6^*ts !o\*le eql !.?uEa pw uounquorsl66qruaop ploH qrEA 6qr 6n ld oo) 'do6 .ql ol0r u6o-pueq ued 6aot .||edrd eql da.s 'uEdffolaqt uo s@6
olur ! oprrsp8 Fqla60r ! ssad uors'd €qr F^o ( r) Fppq uoFrd 614..erd u?d FAor €u€d'doql orlr (r) 6uids qlh {a)uorsd el]l u4u1 -
r!!s*u
r €.etd{ plE {r) 6u"ds qlh (,) uols'd e']|4o@u
-
6!ud. ol r..!qE rl Dpd .u
r$!.€t
V
I e^ous puEr.qF6ol (!) iappq uoFdoqlsd
1p.pr^ord!c!oa.qt lo V uorrsode6n'Aressu lL)
uq eqtot t oorurnlIq du6 €qr uot (01)uedEdq€u.d'd oql mGun '.sts pue suored6!edea. € uor€s *
:ppou -lu o t - r €irl s' uoFl€ox€ uv
eoo, 6uFn {?L)e/€q. uo[sl6
€ql go I nd puPoD6dd eql p .p!s .ql uo uou.q uorl-1. .\l] uaop ploH o rlod / I 6tj 4qul.s
p!. {ql/,6!qo
v
au.dd P$ds rct suBd .'l'..ds .cqda ol aoq @ suo l.tulsu Iq poaollol sl
p lo uo&usop Fr6!€6 v
spou t€!ueq5€t6u6| e iot I qusse puBI qu66
FnUEUsrquo{t 6'l) e e6eds 6eqd
rFlirrP.ar
I
tffi
r!1r_. 4,-10O!L:i !Fi
sasss-rre ih! p Felb
.s.. sd e B.l lh c lr g ,I
:.e s! 1 s.e a .r 1 !,,1 ,r .r
'i:
,, rl r.rr)..r IFF nl e na sp nq
i r ,- .p r .L, r rr , r. l .rn.,.p:.e
I
'r- rr, ,r 1r r,Lf.i r,:ri sea
r,11r-i o-.i
1!:..:aji..5
5..- r - .!p,ir lF.!..i
Ds as s e- : i
1-
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l
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.:,
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33
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:h€ ock'ig m€nan'sh 15arwhich,s ocaredonlhe cyrnderanachmenr
, l5c r ewc y lnde. 1rl) t o m r h ec y n d e . a n a c h m e l 5 t b y t u h n q l o r h e e i l
rJl thedsassembyad supp€d overthecy nder
a{acrrmentisl t6m abov€
$h crr pushesr,repsro. dow.
.*phedsssemby
ad dep.essedon rh€cyrnderaxacnmenrnlne owesr
::s ron us ng yourrorchneer
a.d lhumba.d lse the orherhandto p! torl lhe
leep rhedsassembyad dep@ssed
and pusha.ew p srononto pisio.
-rd 12)uprolh€slop Fehoverhed sassembry
ad
;.rew cy,nderll1)back up I ghi y so rhsl llre grcovesor rhecy,nderandlhe
, . c e €k ng dev 6er 5a ) o nc y r n d e ar n a c h m e . l
l 5 r i o m a o o v es o r h a r h e
rgs €xre.o nrorie qrooves
Pll er ec r ons eev e( t 2r o nt h € r o w e r p a na n dh o d s t e a d y
l 0 r n r oq r p w r h o n eh a n d ! s n q r n eo r h e r r o n i
Sc€w Lprhe otre. pad othe p,p€nerrnny
ns er lanes f r er r nec e s s a r y
f.;'
',::El?T r:EI]l
rT,:ir.r=-
A F e p lr c' n g th e O.fin g s
L r ,.r ,a
:l.r !r ,1 .,
T :]' ].:ir .' r .]il.ll].
B RPpric ng lhe p,ston se. s
,
!
The lwo slop hooks o. trr. rrsd.
p la te ( F iq 2 ' 7 ) pops up
are delr.ssed
and lhe cover
1- : ar r . m y .a s s h o w n , nF g 7
' - I : 1r € r oo u n d e r t h em e r ac p a n ds 'd e l u p w a d s
' _ - ", . ( er pan un r ] l h e s e o n dc 'p t s J a . , n qr o e a 'n s w h e nd o n g s o
* €r r hor der wih!o ! , l h u m b l r h e r p r o d e , s s ! b , € l l o s p r n 9 i e n s o n l
t-:
re\ er on rhe secondc p t@mthe saroiyhook.
! ! ot riheI p hoder ( F S 3 l
Io cl.ghlhepiston pur ofi|re sp.ngsrtheprsronswerede berareryoad€d
Jneleny, vrpe a psro^s ls$
Re attachlhe s
60 :t isopropano as r€!!
'e'r
and ubncare
ngsand lurn lheD s ghly
Azangemenr
of rhep slons
A-ch.nnel 0.5 - 10!L
a- c h. nn. l 10- 1@ ! L / 3 0 - 3 o o ! L
l2'chan..l 0.5 10!L
12- c hann6l10- 10Ot r l/ 3 0 - 3 0 O y L
TheI p hoderconlans lhe Jo os,no lor eachchanne pressp ece s
spf ng paie yrrh 1rr€p sroi sea under.earhlFq 9l
ng and
To remoret'reseas o.n !o!r nd€i I rqer ontolrreopen'ngorlhechanne
'n
/i\
th. pess p'oc.
's
underspn.g tension.nd may pop out dunng
0. 5- lO pL and 10- 100p I
P, es s t her l r e p. . . r s d e A i o i r i € r . . . i o r h e o p e ^ . g s o i l h e r ! i r o r d e r
and s derhe pr€sspece up*3rds s qhr ! Th s oos..srhe presspece a.d {
can be @mo!€n1Flq r0i
87
O - $O ! l
- P@6d aB w h rh€ oth€r B26!. Th€ DBss or&o is disrd€d.
- usino srd6 C ot th6 l@1,pulr od the 3pnh9, st ing pldE and $l
aid Bplace lhe dolecliv. 3€al.
(Fig. 11a)
0,5- lO pl .nd l0- t00 r[
- Lo.d sid6 c ol tho t@l wnh lhe p'6 p€cr, rh6 tha spnhg. tns rE sp.iog
plale a.d lh6 ti€ eal. RFiBdi side c rnrorh. tip hold* and pr* il r.
(Fig. 11b)-Make 3u€ that tha pbss , 4a is In lh. co@t posilon.
O-llt
!L
- Pl&o 6 |w
D|a pieo onto rh€ bo| ard orda€d as wrth ihe othd si:6
- The p6 pie. locl€ into placo in lhs opsrng ol h€ np holder.
- Slido th6 tip holdr comdordy ovd rhs pislon. whM do'no e, hold d a
- lJsing t@r thomb, p'F rh. fi6t chp fifrly upwar& ov& the $tett h@k.
Th{ otat. ih€ lowd pan, p€sing lhe lip hold6r agaiBt rhe uppd p*l whF
doin! e. usrngyoorrhlhb, p6s lhe sond clp nmry osm. hook.
- slid6 on rh€ ho6ng, pcs rhe $op h@ks logElhd srighirydd p€s dom
tn6 covd olale until l@ks into dee
'l
q oy o$.. n!.dduoir|
p.rt h.{ b.r h.rLd
An rt-.p-.
h.r b-n D.d., rh. plp.ttc mu.t b. t..t d to a6 wharl|.. it l.
nhcddlng co@cit (olib6$d
lrclu<Ld).
|l It|g b.hrcttoi.
tq .llDiErinq t ult p|!v. to b. 0*cc1!1,
sd rha plFtta to your authorizad .|..1.. ld npali
lir
. ie Page3 Fq 1 ol t r s m a . r a
' r e. s ur elr r €c ons s t €nr q !lar . f t h e p p e f l e s o € , e c o n r m e n! sd n g l r r €
: rrd na pansor lhe sameg€neraton
lare Concepl* Prysoca,e ConceprStancad - Slandaro.
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