Zhang, Hui Hui Zhang, Ph. D. Associate Professor

Zhang, Hui
Curriculum Vitae
Name:
Hui Zhang, Ph. D.
Title and address:
Associate Professor
Department of Chemistry and Biochemistry
College of Sciences
SEB4138, Mail Box 454003
University of Nevada, Las Vegas
4505 S. Maryland Parkway
Las Vegas, NV 89154-4003, USA
Email: [email protected]
Citizenship:
United States of America
Education:
B.S. in Biology, Peking University, Beijing, China, July, 15, 1982
Ph. D. in Biological Chemistry, The Johns Hopkins University,
School of Medicine, Baltimore, Maryland., USA, May, 30, 1990
Career:
July, 1, 1990-October 14, 1991
Postdoctoral Associate
Department of Cell Biology
Harvard Medical School, Boston, USA
October 15, 1991-1995
Postdoctoral Associate
Howard Hughes Medical Institute
Cold Spring Harbor Laboratory
Cold Spring Harbor, New York, USA
July 1, 1996-June 30, 2002
Assistant Professor
Department of Genetics
Yale University School of Medicine, New Haven, USA
2002, July-June 30, 2007
Associate Professor
Department of Genetics
Yale University School of Medicine, New Haven, USA
July 1, 2007-July 31, 2012
Senior Member
Director of Cancer Cell Proteomics
Nevada Cancer Institute
Las Vegas, Nevada, USA
August 1, 2012-present
1
Zhang, Hui
Associate Professor
Department of Chemistry
University of Nevada, Las Vegas
Las Vegas, NV 89154-4003
Professional Honors or Recognition:
1982
Graduated with Summa Cum Laude, Peking University,
Beijing, China
1993
Associateship, Howard Hughes Medical Institute, Cold Spring
Harbor Laboratory, Cold Spring Harbor, New York
1996:
Donaghue Medical Research Foundation New Investigator
Award
1998:
Junior Faculty Scholar Award, American Cancer Society
2004:
Connecticut State Breast Cancer Research Award
Committees and Reviewers:
1995-present
Frequent reviewer for biomedical journals Nature, Cell,
Science, Molecular Cell, Cancer Cell, Developmental Cell,
Nature Genetics, Genes & Development, EMBO J.,
Molecular Cell Biology, Molecular Biology of Cell,
Proceeding of the National Academy of USA, Journal of Cell
Biology, Cancer Research, JBC, PLoS Biology, etc
2014:
Editorial Board Member of Stem Cell Epigenetics
2009-2012
Member of Scientific Leadership Council
Nevada Cancer Institute
2009-2012
Member of Core Facility Committee
Nevada Cancer Institute
2009-2012
Member of Faculty Promotion Committee
Nevada Cancer Institute
Bibliography:
1) Zhang, H., Wang, J. C., and Liu, L. F. (1988). Involvement of DNA topoisomerase I in
transcription of human ribosomal RNA genes. Proc. Natl. Acad. Sci. USA 85, 1060-1064. PMCID:
PMC279701
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Zhang, Hui
2) Hiyam, H. E., Zhang, H., and Weiss, B. (1988). Lethality of a dut (deoxyuridine
triphosphatase) mutation in Escherichia coli.. J. Bacteriol. 170, 1069-1075. PMCID: PMC210875
3) Juan, C-C., Hwang, J., Liu, A. A., Whang-Peng, J., Knutsen, T., Huebner, K., Croce, C. M.,
Zhang, H., Wang, J. C., and Liu, L. F. (1988). Human DNA topoisomerase I is encoded by a
single-copy gene that maps to chromosome region 20q12-13.2. Proc. Natl. Acad. Sci. USA 85,
8910-8913. PMCID: PMC282616
4) Yang, L., Jessee, C. B., Lau, K., Zhang, H., and Liu, L. F. (1989). Template supercoiling
during ATP-dependent DNA helix tracking: studies with simian virus 40 large tumor antigen.
Proc. Natl. Acad. Sci. USA 86, 6121-6125. PMCID: PMC297787
5) Zhang, H., D"Arpa, P. and Liu, L. F. (1990). A model for tumor cell killing by topoisomerase
poisons. Cancer Cells (Cold Spring Harbor Laboratory Press). January: 23-27. PMID:2167111
6) Zhang, H., Jessee, C. B., and Liu, L. F. (1990). A protein from Xenopus oocytes with simian
virus 40 large tumor antigen-like DNA supercoiling activity. Proc. Natl. Acad. Sci. USA 87, 90789082. PMCID: PMC55107
7) Xiong, Y., Zhang, H., and Beach, D. (1992). D type cyclins associate with multiple protein
kinases and the DNA replication and repair factor PCNA. Cell 71, 505-514. PMID:1358458
8) Zhang, H. and Ruderman, J. V. (1993). Differential replication capacities of G1 and S-phase
extracts from sea urchin eggs. J. of Cell Science 104, 565-572. PMID:8505379
9) Zhang, H., Xiong, Y., and Beach, D. (1993). Proliferating cell nuclear antigen and p21 are
components of multiple cell cycle kinase complexes. Mol. Biol. Cell 4, 897-906. PMCID:
PMC275720
10) Xiong, Y., Zhang, H., and Beach, D. (1993). Subunit rearrangement of the cyclin-dependent
kinases is associated with cellular transformation. Genes & Devel. 7, 1572-1583. PMID:8101826
11) Xiong, Y., Hannon, G. J., Zhang, H., Casso, D., Kobayashi, R., and Beach, D. (1993). p21 is a
universal inhibitor of cyclin kinases. Nature 366, 701-704. PMID:8259214
12) Demetrick, D. J., Zhang, H., and Beach, D. (1994). Chromosomal mapping of human CDK2,
CDK4 and CDK5 cell cycle kinase genes. Cytogenet Cell Genet 66, 72-74. PMID:8275715
13) Zhang, H., Hannon, G., J., and Beach, D. (1994). p21-containing cyclin kinases exist in both
active and inactive states. Genes & Devel. 8, 1750-1758. PMID:7958854
14) Zhang, H., Hannon, G., J., Casso, D., and Beach, D. (1994). p21 is a component of active cell
cycle kinases. In Molecular Genetics of Cancer, Cold Spring Harbor Laboratory Symposium on
Quantitative Biology, volume LIX, 21-29.PMID:7587072
15) Demetrick, D.,Matsumoto, S., Hannon, G., Okamoto, K., Xiong, Y., Zhang, H., and Beach, D.
(1994). Chromosomal mapping of the genes for the human cell cycle proteins cyclin C (CNNC),
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Zhang, Hui
cyclin E (CNNE), p21 (CDKN1) and KAP3 (CDKN3). Cytogenet Cell Genet 69, 190-192.
PMID:7698009
16) Zhang, H., Kobayshi, R., Galaktionov, K., and Beach, D. (1995). p19Skp1 and p45Skp2 are
essential elements of cyclin A/CDK2 S-phase promoting kinase. Cell 82, 915-925. PMID:
7553852
17) Demetrick, D. J., Zhang, H., and Beach, D. (1996). Chromosomal mapping of the genes for
the human CDK2/cyclin A-associated proteins p19 (SKP1A and SKP1B) and p45 (SKP2).
Cytogenetics & Cell Genetics 73,104-107. PMID: 8646875
18) Demetrick, D. J., Zhang, H., and Beach, D. (1996). Chromosomal mapping of the genes for
the human CKS1 (CKShs1) and CKS2 (CKShs2). Cytogenetics & Cell Genetics. Cytogenetics &
Cell Genetics 73, 250-254. PMID: 8697818
19) Gervais, J. Seth, P., and Zhang, H. (1998). Cleavage of the CDK inhibitor
p21Cip1/Waf1 by caspases is an early event during DNA damage-induced apoptosis. J. Biol.
Chem. 273, 19207-19212. PMID: 9668108
20) Yu, Z.-K., Gervais, J., and Zhang, H. (1998). Human CUL-1 associates with the
SKP1/SKP2 complex and regulates p21CIP1/WAF1 and cyclin D proteins. Proc. Natl. Acad.
Sci. USA 95, 11324-11329. PMCID: PMC21641
21) al-Ramadi, B. K., Zhang, H., and Bothwell, A. L. M. (1998). Cell cycle arrest and
apoptosis hypersusceptibility as a consequence of p56lck deficiency in non-transformed T
lymphocytes. Proc. Natl. Acad. Sci. USA 95, 12498-12503. PMCID: PMC22859
22) Naeger, L.K., Hwang, E, -S, Zhang, H. and DiMaio, D. (1999). Bovine papillomavirus
E2 protein activates a complex growth-inhibitory program in p53-negative HT-3 cervical
carcinoma cells that induces repression of cyclin A and cdc25A and cdc25B phosphatase
genes and accumulation of hypophosphorylated retinoblastoma protein. Cell Growth and
Differentiation 10, 413-422. PMID: 10392903
23) Sun, H., Lesche, R., Li, D.-M., Lilienta. J., Zhang, H., Gao, J., Garvarina, N., Mueller, B.,
Liu, X., and Wu, H. (1999). PTEN modulates cell cycle progression and cell survival by
regulating PIP3 and Akt/PKB signaling pathway. Proc. Natl. Acad. Sci. USA 96, 6199-6204.
PMCID: PMC26859
24) Tsvetkov, M. L., Yeh, K.-H., Lee, J.-S., Sun, H., and Zhang, H. (1999). p27Kip1
ubiquitination and degradation is regulated by the SCFSKP2 complex through phosphorylated
Thr187 in p27. Current Biology 9, 661-664. PMID: 10375532
25) Duann, P. Sun, M., Lin, C.-T., Zhang, H., Liu, L. F. (1999). Plasmid linking number
change induced by topoisomerase-mediated DNA damage. Nucl. Acids Res. 27, 2905-2911.
PMCID: PMC148505
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Zhang, Hui
26) Yokouchi, M., Houghton, A., Kondo, T., Bartkiewicz, M., Horne, W. C., Zhang, H.,
Yoshimura, A., and Baron, R. (1999). Ligan-induced Ubiquitination of the EGF Receptor
Involves the Interaction of the c-Cbl Ring Finger and UbcH7. J. Biol. Chem. 274, 3170731712. PMID: 10531381
27) O'Connor, D., Grossman, D., Plescia, J., Li, F-Z., Zhang, H., Villa, A., Tognin, S.,
Marchisio, P. C., and Dario C. Altieri (2000). Regulation of apoptosis at cell division by
p34cdc2 phosphorylation of survivin. Proc. Natl. Acad. Sci. USA 97: 13103-13107. PMCID:
PMC27185
28) Sun, M., Duann, P., Lin, C.-T., Zhang, H., and Liu, L. F. (2000). Rapid Chromatin
Reorganization Induced by Topoisomerase I-Mediated DNA Damage. Annals New York
Acad. Sci. 2000;922:340-2. PMID: 11193917
29) Duan, H., Tsvetkov, L. M., Liu, Y., Song, Y., Swaroop, M., Wen, R., Kung, H.-Fu.,
Zhang, H., and Sun, Y. (2001). Promotion of the S-phase entry and cell growth under serum
starvation by SAG/ROC2/Rbx2/Hrt2, an E3 ubiquitin ligase component: Association with
inhibition of p27 accumulation. Molecular Carcinogenesis 30, 37-46. PMID: 11255262
30) Mamillapalli, R., Gavrilova, N., Mihaylova, V. T., Tsvetkov, L. M., Wu, H., Zhang, H.,
and Sun, H. (2001). PTEN Regulates the Ubiquitin-dependent Degradation of the CDK
inhibitor p27KIP1 through the Ubiquitin E3 Ligase SCFSKP2. Current Biology, 11, 263-267.
PMID: 11250155
31) Yeh, K.-H., Kondo, T., Zheng, J.-Y., Tsvetkov, L. M., Blair, J., and Zhang, H. (2001).
The F-Box Protein SKP2 Binds to the Phosphorylated Threonine 380 in Cyclin E and
Regulates Ubiquitin-Dependent Degradation of Cyclin E. Biochemical and Biophysical
Research Communications Mar 9;281(4):884-90. PMID: 11237742
32) Yokouchi, M., Kondo, T., Sanjay, A., Houghton, A., Yoshimura, A., Zhang, H., and
Baron, R. (2001). Cbl’s Phosphorylation upon Interaction with Src Leads to the
Interdependent Ubiquitination of Both Proteins. J. Biol. Chem. 276, 35185-35193. PMID:
11448952
33) Mihaylov, I., Kondo, T., Jones, L., Ryzhikov, S., Tanaka, J., Zheng, J.-Y., Higa,
L. A., Minamino, N., Cooley, L., and Zhang, H. (2002). Control of DNA Replication
and Chromosome Ploidy by Geminin and Cyclin A. Mol. Cell Biol. 22, 1868-1880.
PMCID: PMC135598
34) Yang, X., Menon, S., Lykke-Andersen, K., Tsuge, T., Xiao, D., Rodriguez-Suarez,
R.J., Zhang H. and Ning Wei (2002). The COP9 signalosome inhibits ubiquitindependent degradation of p27Kip1 and negatively regulates cell cycle at G1 phase via
de-neddylation of SCF Cul1. Current Biol. 12, 667-672. PMID: 11967155
35) Zheng, J., Yang, X., Harrell, J., Ryzhikov, S., Shim, E., Lykke-Andersen, K., Wei, N.,
Sun, H., Kobayashi, R., and Zhang, H. (2002). CAND1 Binds to Unneddylated CUL1 and
Regulates the Formation of SCF Ubiquitin E3 Ligase Complex. Molecular Cell 10, 15191526. PMID: 12504026
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Zhang, Hui
36) Shim, E., Ryzhikov, S., Noh, H., Kim, Y., Zeiss, C., and Zhang, H. (2003). Expression of
the F-box Protein SKP2 Induces Hyperplasia, Dysplasia, and Low Grade Carcinoma in the
Mouse Prostate. Cancer Research 63, 1583-1588. PMID: 12670908
37) Higa, L.A. Mihaylov, I., Banks, D., Zheng, J. and Zhang, H. (2003) Radiation-Mediated
CDT1 Proteolysis by CUL4-ROC1 and COP9-Signalosome Complexes Constitutes a New
Checkpoint, Nature Cell Biology 5, 1008-1015. PMID: 14578910
38) Higa, L. A. and Zhang, H. (2004) The SCF Ubiquitin E3 Ligase. In Protein Degradation,
by Wiley-VCH Winheim, John Mayer, Aaron Ciechanover, and Martin Rechsteiner (Eds),
Volume 1, 135-155.
39) Higa, L.A. Yang, X., Zheng, J., Banks, D., Wu, M., Ghosh, P., Sun, H., and Zhang, H.
(2006). Involvement of CUL4 Ubiquitin E3 Ligases in Regulating CDK Inhibitors
Dacapo/p27Kip1 and Cyclin E Degradation. Cell Cycle 5:1, 71-77. PMID: 16322693
40) Hulit, J., Lee, R. J., Li, Z., Wang, C., Katiyar, S., Yang, J., Quong, A. A., Wu, K.,
Albanese, C., Russell, R., Di Vizio, D., Koff, A., Thummala, S., Zhang, H., Harrell, J., Sun,
H., Muller, W. J., Inghirami, G., Lisanti, M. P., Pestell, R. G. (2006). P27Kip1 Repression of
ErbB2-Induced Mammary Tumor Growth in Transgenic Mice Involves Skp2 and
Cancer Research 66, 8529-8541. PMID: 16951165
41) Higa, L. A., Banks, D, Wu, M., Kobayashi, R., Sun, H., Zhang, H. (2006). L2DTL/CDT2
Interacts with the CUL4/DDB1 Complex and PCNA and Regulates CDT1 Proteolysis in
Response to DNA Damage. Cell Cycle 5:15, 1675-1680. PMID: 16861906
42) Banks, D., Wu, M., Higa, L. A., Gavrilova, N., Guan, J., Ye, T., Kobayashi, R., Sun, H.,
Zhang, H. (2006). L2DTL/CDT2 and PCNA interact with p53 and regulate p53
Polyubiquitination and Protein Stability through MDM2 and CUL4A/DDB1Complexes, Cell
Cycle 5:15, 1719-1729. PMID: 16861890
43) Higa, L. A., Wu, M., Ye, T., Kobayashi, R., Sun, H., Zhang, H. (2006). CUL4/DDB1
Ubiquitin Ligase Interacts with Multiple WD40-repeat Proteins and Regulates Histone
Methylation. Nature Cell Biology 8:1277-1283. PMID: 17041588
44) Pamarthy, D., Tan, M., Wu, M., He, H., Chen, J., Yang, D., Wang, S., Zhang, H., and
Sun, Y. (2007). P27 Degradation by an Ellipticinium Series of Compound via UbiquitinProteasome Pathway. Cancer Biology & Therapy March 6:3. PMID: 17312389
45) Zhang, H. (2007). Life without Kinase: Cyclin E Promotes DNA Replication Licensing
and Beyond. Molecular Cell 25, 175-176. PMID: 17244524
46) Higa, L. A. and Zhang, H. (2007). Stealing the Spotlight: CUL4-DDB1 Ubiquitin
Ligase Docks WD40-Repeat Proteins to Destroy. Cell Division Feb 6; 2(1):5. PMCID:
PMC1805432
47) Jonason, J. H., Gavrilova, N., Wu, M., Zhang, H., Sun, H. (2007). Regulation of
SCF(SKP2) Ubiquitin E3 Ligase Assembly and p27(KIP1) Proteolysis by the PTEN
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Zhang, Hui
Pathway and Cyclin D1. Cell Cycle 2007 Apr 5; 6(8). PMID: 17438373
48) Minoda, R., Izumikawa, M., Kawamoto, K., Zhang, H., Raphael, Y. (2007).
Manipulating Cell Cycle Regulation in the Mature Cochlea. Hearing Research, Oct;232(12):44-51. PMCID: PMC204857
49) Ghosh, P., Wu, M., Zhang, H., and Sun, H. (2008). mTORC1 signaling requires
proteasomal function and the involvement of CUL4-DDB1 ubiquitin E3 ligase. Cell Cycle
7:3, 373-381. PMID: 18235224
50) Ren, Q., Dai., L., Zhang, H., Tan, T., Xu, Z., and Ye, Y. (2008). Total Synthesis of
Largazole. Synlett 15, 2379-2383.
51) Hui Zhang (2008). Regulation of Cell Cycle by Cullin Ubiquitin E3 Ligases. Trends in
Cell Cycle Research 125-148, Editor: Kenichi Yoshida, Research Signpost, SSBN: 978-81308-0274-9.
52) Xiang, J., Yang, H.-B., Che, C., Zou, H.-X., Yang, H.-S., Chen, J.-H., Zhang, B., Wei,
Y.-Q., Quan, J.-M., Zhang, H., Yang, Z., and Lin, S. (2009). Identifying Tumor Cell Growth
Inhibitors by Combinatorial Chemistry and Zebrafish Assays. PLoS One 4, e4361. PMCID:
PMC2633036
53) Yuqing Liu, Xiao Ming Wen, Eric Lik Hang Lui, Scott L Friedman, Wei Cui, Nancy Pei
Shan Ho, Lei Li, Tao Ye, Sheung Tat Fan and Hui Zhang. (2009). Therapeutic targeting of
the PDGF and TGF--signaling pathways in hepatic stellate cells by PTK787/ZK22258.
Laboratory Investigation 89, 1152–1160. PMCID: PMC2891536
54) Wu, X.-H., Zhang, H., and Wu, Y.-D. (2009). Is Asp-His-Ser/Thr-Trp Tetrad Hydrogenbond Network Important to WD40-repeat Proteins: A Statistical and Theoretical Study.
PROTEINS: Structure, Function, and Bioinformatics (Johns Hopkins University Publication)
78, 1186-1194. PMID:19927323
55) Bo Chen, Lu Dai, Hui Zhang, Wenfei Tan, Zhengshuang Xu and Tao Ye (2010).
Towards the Stereochemical Assignment of Natural lydiamycin A. Chem. Commun.,
2010, 46, 574-576. DOI: 10.1039/B922544G
56) Zhang, H. (2010). Skip the Nucleus, AKT Drives Skp2 and FOXO1 to the Same Place?
Cell Cycle, March 1, 9(5). PMID: 20348850
57) Liu Y, Wang Z, Kwong SQ, Lui EL, Friedman SL, Li FR, Lam RW, Zhang GC, Zhang,
H, Ye T. (2011). Inhibition of PDGF, TGF-β, and Abl signaling and reduction of liver fibrosis
by the small molecule Bcr-Abl tyrosine kinase antagonist Nilotinib. Journal of Hepatology
55(3):612-25. PMID: 21251937 (co-corresponding author)
58) Guimiao Lin, Lijuan Zhao, Feng Yin, Rongfeng Lan, Lianbo Li, Xiaoming Zhang, Hui
Zhang, Baoxue Yang (2011). TCF3 inhibits F9 embryonal carcinoma growth by the downregulation of Oct4. Oncology Reports 26, 893-899. PMID:21725619.
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59) Jing Wang, Fei Lu, Qi Ren, Hong Sun Zhengshuang Xu, Rongfeng Lan, Yuqing Liu
,Xiaoming Wen, David Ward, Junmin Quan, Tao Ye, Hui Zhang. (2011). Novel Chemicals
Selectively Inhibit Histone Demethylase LSD1 and Target Tumor Cells with Pluripotent Stem
Cell Properties. Cancer Research 71(23), 7238-49. PMID: 21975933.
60) Rongfeng Lan, Guimiao Lin, Feng Yin, Jun Xu, Xiaoming Zhang, Jing Wang, Yanchao,
Wang, Jianxian Gong, Yuan-Hua Ding, Zhen Yang, Fei Lu, and Hui Zhang (2012).
Dissecting the Phenotypes of Plk1 Inhibition in Cancer Cells Using Novel Kinase Inhibitory
Chemical CBB2001. Laboratory Investigation 92, 1503-14. PMID: 22890557.
61) Liu Y, Wang Z, Wang J, Lam W, Kwong S, Li F, Friedman SL, Zhou S, Ren Q, Xu Z,
Wang X, Ji L, Tang S, Zhang H, Lui EL, Ye T. (2013). A histone deacetylase inhibitor,
largazole, decreases liver fibrosis and angiogenesis by inhibiting transforming growth factor-β
and vascular endothelial growth factor signalling. Liver International 33(4):504-15.
PMID: 23279742
62) Dai L, Liu Y, Liu J, Wen X, Xu Z, Wang Z, Sun H, Tang S, Maguire AR, Quan J, Zhang
H, Ye T. (2013). A novel CyclinE/CyclinA-CDK Inhibitor targets p27(Kip1) degradation, cell
cycle progression and cell survival: Implications in cancer therapy. Cancer Letter, 333(1):10312. PMID: 23354589
63) Long B, Tang S, Chen L, Qu S, Chen B, Liu J, Maguire AR, Wang Z, Liu Y, Zhang H,
Xu Z, Ye T. (2013). Total synthesis of padanamides A and B. Chem Commun
(Camb);49(29):2977-9. PMID: 23446820
64) Liu H, Liu Y, Wang Z, Xing X, Maguire AR, Luesch H, Zhang H, Xu Z, Ye T. (2013).
Total synthesis and biological evaluation of grassypeptolide a. Chemistry;19(21):6774-84.
doi: 10.1002/chem.201203667. PMID: 23536467
65) Xiaoming Zhang, Fei Lu, Jing Wang, Feng Yin, Zhengshuang Xu, Dandan Qi, XiaYuwen
Cao, Weihua Liang, Yuqing Liu, Hong Sun, Tao Ye, and Hui Zhang (2013). Pluripotent
Stem Cell Protein Sox2 Confers Sensitivity towards LSD1 Inhibition in Cancer Cells. Cell
Reports. 2013 Oct 15. doi:pii: S2211-1247(13)00541-X. 10.1016/j.celrep.2013.09.018. [Epub
ahead of print]. PMID: 24139802.
66) Feng Yin, Rongfeng Lan, Xiaoming Zhang, Linyu Zhu, Fangfang Chen, Zhengshuang Xu,
Yuqing Liu, Tao Ye, Hong Sun, Fei Lu, and Hui Zhang (2014). LSD1 Regulates Pluripotency
of Embryonic Stem/Carcinoma Cells through HDAC1-mediated Deacetylation of Histone H4
at Lysine 16. Mol. Cell Biol., 34(2):158-79. doi: 10.1128/MCB.00631-13. Epub 2013 Nov 4.
PMID: 24190971
67) Zhang, Hui (2014) Cullin Ubiquitin E3 Ligases, in Encyclopedia of Cancer, Springer
Publishing, in Press.
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