RC IC and LD Values for RC Substances with Rat Oral LD

In Vitro Cytotoxicity Test Methods BRD Appendix K3
November 2006
RC IC50 and LD50 Values for RC Substances with Rat Oral LD50 Data
Substance
Formaldehyde
Methanol
Acetonitrile
Sodium I fluoride
Lithium I chloride
Acetaldehyde
Ethanol
Ammonium sulfide
Acrylonitrile
Ammonium chloride
Acrolein
Propionaldehyde
Allylalcohol
Acetone
Potassium I fluoride
Sodium chloride
Acetic acid
1-Propanol
2-Propanol
Ethylene glycol
Sodium azide
Potassium cyanide
Acrylamide
n-Butanal
Isobutanal
Ethyl methyl ketone
Dimethylformamide
Isobutanol
1-Butanol
Potassium I chloride
Thioacetamide
2-Methoxyethanol
Propylene glycol
Thiourea
Dimethyl sulfoxide
Pyridine
Dichloromethane
Piperazine
N,N-Dimethylacetamide
1,4-Dioxane
Molecular
Weight
(g/mole)
30.03
32.05
41.06
41.99
42.39
44.06
46.08
51.12
53.07
53.5
56.07
58.09
58.09
58.09
58.1
58.44
60.06
60.11
60.11
62.08
65.02
65.12
71.09
72.12
72.12
72.12
73.11
74.14
74.14
74.55
75.14
76.11
76.11
76.13
78.14
79.11
84.93
86.16
87.14
88.12
IC50x
(mM)1
0.12
930
368
1.85
38.6
2.45
379
0.42
2.42
5.52
0.047
3.25
6.94
444
3.13
75.9
24.3
96.5
167
555
0.71
1.12
1.61
12.8
13.5
104
114
40.1
52.5
82
4.17
251
342
86
252
46.9
34.9
67.2
24.2
38.1
K-25
IC50x
(mg/mL)1
Rat Oral
LD50
(mmol/kg)2
3.60
29806.5
15110.1
77.68
1636.3
107.95
17464.3
21.47
128.43
295.32
2.64
188.79
403.14
25792.0
181.85
4435.6
1459.5
5800.6
10038.4
34454.4
46.16
72.93
114.45
923.1
973.62
7500.5
8334.5
2973.0
3892.4
6113.1
313.33
19103.6
26029.6
6547.2
19691.3
3710.3
2964.1
5790.0
2108.8
3357.4
26.6
406
92.5
4.29
17.9
43.8
304
3.29
1.54
30.8
0.82
24.3
1.1
168
4.22
51.3
55.1
89.8
97.2
138
0.69
0.15
2.39
34.5
39
47.1
38.3
33.2
10.7
34.9
4.01
32.3
263
1.64
252
11.3
18.8
22.1
58.4
47.7
Rat Oral
LD50
(mg/kg)2
798.8
13012.3
3798.1
180.1
758.8
1929.8
14008.3
168.2
81.7
1647.8
46.0
1411.6
63.9
9759.1
245.2
2998.0
3309.3
5397.9
5842.7
8567.0
44.9
9.8
169.9
2488.1
2812.7
3396.9
2800.1
2461.4
793.3
2601.8
301.3
2458.4
20016.9
124.9
19691.3
893.9
1596.7
1904.1
5089.0
4203.3
In Vitro Cytotoxicity Test Methods BRD Appendix K3
November 2006
RC IC50 and LD50 Values for RC Substances with Rat Oral LD50 Data
Substance
Ethyl acetate
1-Pentanol
1-Nitropropane
Lactic acid
1,3,5-Trioxane
Glycerol
Toluene
Aniline
Phenol
Sulfuric acid
Chromium VI trioxide
2-Ethylbutanal
Cyclohexanol
Tetrahydrofurfuryl alcohol
1-Hexanol
Styrene
Sodium I bromide
Beryllium II sulfate
Diethylene glycol
Xylene
p-Cresol
o-Cresol
m-Cresol
Benzylalcohol
Anisole
p-Phenylenediamine
o-Phenylenediamine
p-Aminophenol
m-Aminophenol
Catechol
Resorcinol
Calcium II chloride
Trifluoroacetic acid
2,5-Hexanedione
1-Heptanol
Sodium monochloroacetate
2-Butoxyethanol
Chloroform
Benzoic acid
Nicotinamide
Molecular
Weight
(g/mole)
88.12
88.17
89.11
90.09
90.09
92.11
92.15
93.14
94.12
98.08
100
100.18
100.18
102.15
102.2
104.16
104.92
105.07
106.14
106.18
108.15
108.15
108.15
108.15
108.15
108.16
108.16
109.14
109.14
110.12
110.12
110.98
114.03
114.16
116.23
116.48
118.2
119.37
122.13
122.14
IC50x
(mM)1
128
24.9
57.9
66
213
624
17.1
6.9
3.01
36
0.0027
13.2
26.3
111
15.4
3.3
77.4
0.61
62.1
12
0.22
0.52
0.66
5.81
13.2
0.05
0.31
0.062
0.86
0.2
0.8
12.4
20.5
8.45
6.25
1.45
26
13.4
15.7
44.4
K-26
IC50x
(mg/mL)1
Rat Oral
LD50
(mmol/kg)2
11279.4
2195.4
5159.5
5945.9
19189.2
57476.6
1575.8
642.67
283.30
3530.9
0.27
1322.4
2634.7
11338.7
1573.9
343.73
8120.8
64.09
6591.3
1274.2
23.79
56.24
71.38
628.35
1427.6
5.41
33.53
6.77
93.86
22.02
88.10
1376.2
2337.6
964.65
726.44
168.90
3073.2
1599.6
1917.4
5423.0
125
34.4
5.11
41.4
8.88
137
54.3
4.72
4.4
21.8
0.8
39.7
20.6
24.5
7.04
48
33.4
0.78
139
40.5
1.91
1.12
2.24
11.4
34.2
0.74
9.89
15.2
15.2
35.3
2.73
9.01
1.75
23.7
28
0.65
12.5
7.61
20.7
28.7
Rat Oral
LD50
(mg/kg)2
11015.0
3033.0
455.4
3729.7
800.0
12619.1
5003.7
439.6
414.1
2138.1
80.0
3977.1
2063.7
2502.7
719.5
4999.7
3504.3
82.0
14753.5
4300.3
206.6
121.1
242.3
1232.9
3698.7
80.0
1069.7
1658.9
1658.9
3887.2
300.6
999.9
199.6
2705.6
3254.4
75.7
1477.5
908.4
2528.1
3505.4
In Vitro Cytotoxicity Test Methods BRD Appendix K3
November 2006
RC IC50 and LD50 Values for RC Substances with Rat Oral LD50 Data
Substance
p-Toluylendiamine
Nitrobenzene
p-Anisidine
2-Thiouracil
Dichloroacetic acid
Nickel II chloride
Cobalt II chloride
5-Fluorouracil
1,1,1-Trichloroethane
Sodium oxalate
1,2,6-Hexanetriol
Cupric chloride
Zinc II chloride
Salicylamide
Isoniazid
Salicylic acid
p-Nitrophenol
Isononylaldehyde
8-Hydroxyquinoline
Coumarin
N-Methyl-N'-nitro-N-nitrosoguanidine
Isobenzoic furanodione
Thymol
Acetaminophen
Ferrous sulfate
Methyl salicylate
Phenylthiourea
2-Nitro-p-phenylenediamine
Carbon tetrachloride
Menthol
Bromobenzene
Dimethylaminoethyl methacrylate
(polymer)
Strontium II chloride
Sodium salicylate
6-Methylcoumarin
Hydralazine
Nicotine
2,4-Dichlorophenol
Trichloroacetic acid
Molecular
Weight
(g/mole)
IC50x
(mM)1
IC50x
(mg/mL)1
Rat Oral
LD50
(mmol/kg)2
Rat Oral
LD50
(mg/kg)2
122.19
123.12
123.17
128.16
128.94
129.61
129.83
130.09
133.4
134
134.2
134.44
136.27
137.15
137.16
138.13
139.12
142.27
145.17
146.15
0.094
12.2
0.73
0.32
11.5
0.27
0.16
0.0026
10.3
0.44
123
0.11
0.13
1.08
7.49
3.38
0.2
1.52
0.0033
1.71
11.49
1502.1
89.91
41.01
1482.8
34.99
20.77
0.34
1374.0
58.96
16506.6
14.79
17.72
148.12
1027.3
466.9
27.8
216.3
0.48
249.9
0.83
5.2
11.4
7.8
21.9
0.81
0.62
1.77
77.2
1.16
119
1.04
2.57
13.8
4.74
6.45
2.52
22.8
8.27
2
101.4
640.2
1404.1
999.6
2823.8
105.0
80.5
230.3
10298.5
155.4
15969.8
139.8
350.2
1892.7
650.1
890.9
350.6
3243.8
1200.6
292.3
147.12
0.012
1.8
0.61
89.7
148.12
150.24
151.18
151.91
152.16
152.23
153.16
153.81
156.3
157.02
17
0.23
2.71
1.85
1.7
0.54
0.39
8.51
0.95
3.46
2518.0
34.6
409.7
281.0
258.7
82.2
59.7
1308.9
148.5
543.3
27.1
6.52
15.9
2.1
5.83
0.02
20.1
18.2
20.3
17.2
4014.1
979.6
2403.8
319.0
887.1
3.0
3078.5
2799.3
3172.9
2700.7
157.24
0.11
17.3
11.1
1745.4
158.52
160.11
160.18
160.2
162.26
163
163.38
36.4
4.33
0.31
0.33
1.79
0.055
8.19
5770.1
693.3
49.7
52.9
290.4
9.0
1338.1
14.2
9.99
10.5
0.56
0.31
3.56
30.6
2251.0
1599.5
1681.9
89.7
50.3
580.3
4999.4
K-27
In Vitro Cytotoxicity Test Methods BRD Appendix K3
November 2006
RC IC50 and LD50 Values for RC Substances with Rat Oral LD50 Data
Substance
Chloral hydrate
Tetrachloroethene
t-Butyl hydroquinone
(-)-Phenylephrine
m-Dinitrobenzene
Azaserine
1,2-Dibromomethane
L-Ascorbic acid
n-Butyl benzoate
Phenacetin
Iproniazid
Acetylsalicylic acid
D-Glucose
Butylated hydoxyanisole
1,2,4-Trichlorobenzene
Cadmium II chloride
2,4-Dinitrophenol
Undecylenic acid
Tributylamine
Paraquat
Amrinone
Antipyrine
Tin II chloride
Nitrilotriacetic acid
Nitrogen mustard * HCl
Dimethyl phthalate
Caffeine
4-Hexylresorcinol
L-Dopa
Halothane
Arsenic III trioxide
Manganese II chloride *4 H2O
Carbaryl
Sodium cyclamate
Magnesium II chloride * 6 H2O
Phenylephrine * HCl
Triethylene melamine
Ibuprofen
Milrinone
1,3-Bis(2-chloroethyl)- 1-
Molecular
Weight
(g/mole)
165.4
165.82
166.24
167.23
168.12
173.15
173.85
176.14
178.25
179.24
179.25
180.17
180.18
180.27
181.44
183.3
184.12
184.31
185.4
186.25
187.22
188.25
189.59
191.16
192.53
194.2
194.22
194.3
197.21
197.39
197.84
197.92
201.24
201.24
203.33
203.69
204.27
206.31
211.24
214.07
IC50x
(mM)1
2.65
6.54
0.069
4.45
0.39
0.002
4.2
1.52
0.41
1.27
0.79
2.27
226
0.24
0.71
0.0064
0.21
0.18
15.4
0.54
0.28
11.6
1.51
3.61
0.0026
23.4
2.64
0.064
0.13
31.1
0.0042
0.13
0.26
35.4
70.4
4.16
0.00078
0.52
4.77
0.078
K-28
IC50x
(mg/mL)1
Rat Oral
LD50
(mmol/kg)2
438.3
1084.5
11.5
744.2
65.6
0.35
730.2
267.7
73.1
227.6
141.6
409.0
40720.7
43.3
128.8
1.2
38.7
33.2
2855.2
100.6
52.4
2183.7
286.3
690.1
0.50
4544.3
512.7
12.4
25.6
6138.8
0.8
25.7
52.3
7123.9
14314.4
847.4
0.16
107.3
1007.6
16.7
2.9
53.4
4.81
2.09
0.49
0.98
0.62
67.6
28.8
9.21
2.04
5.55
143
12.2
4.17
0.48
0.16
13.6
2.91
0.31
0.54
9.56
3.69
7.69
0.052
35.5
0.99
2.83
9.03
28.8
0.1
7.5
1.24
75.8
39.8
1.72
0.005
4.89
0.43
0.093
Rat Oral
LD50
(mg/kg)2
479.7
8854.8
799.6
349.5
82.4
169.7
107.8
11907.1
5133.6
1650.8
365.7
999.9
25765.7
2199.3
756.6
88.0
29.5
2506.6
539.5
57.7
101.1
1799.7
699.6
1470.0
10.0
6894.1
192.3
549.9
1780.8
5684.8
19.8
1484.4
249.5
15254.0
8092.5
350.3
1.0
1008.9
90.8
19.9
In Vitro Cytotoxicity Test Methods BRD Appendix K3
November 2006
RC IC50 and LD50 Values for RC Substances with Rat Oral LD50 Data
Substance
Molecular
Weight
(g/mole)
IC50x
(mM)1
IC50x
(mg/mL)1
Rat Oral
LD50
(mmol/kg)2
Rat Oral
LD50
(mg/kg)2
nitrosourea
Clofibric acid
Glutethimide
Butylated hydroxytoluene
2,4-Dichlorophenoxyacetic acid
Diethyl phthalate
Bendiocarb
Diethyldithiocarbamate sodium*
3H20
Ammonium persulfate
Cygon
Aminophenazone
Nalidixic acid
Phenobarbital
Ambazone
Mefenamic acid
Triethyltin chloride
Busulphan
Isoproterenol * HCl
Pentobarbital sodium
Cupric sulfate * 5 H2O
2,4,5-Trichlorophenoxyacetic acid
Nabam
Trichlorfon
Natulan * HCl
Diethyl sebacate
Versalide
Secobarbital sodium
Barium II nitrate
Sodium bichromate VI
Theophylline sodium acetate
Maneb
3-Cyano-2-morpholino-5-(pyrid-4yl)-pyridine (Chemical 122)
Pentachlorophenol
Isoxepac
Dichlorophene
Mercury II chloride
Hexachlorocyclopentadiene
Disulfoton
Zineb
214.66
217.29
220.39
221.04
222.26
223.25
2.61
1.56
0.056
0.77
5.52
0.18
560.3
339.0
12.3
170.2
1226.9
40.2
5.82
2.76
4.04
1.67
38.7
0.8
1249.3
599.7
890.4
369.1
8601.5
178.6
225.33
0.00039
0.088
6.66
1500.7
228.22
229.27
231.33
232.26
232.26
237.32
241.31
241.35
246.32
247.75
248.29
249.7
255.48
256.34
257.44
257.8
258.4
258.44
260.3
261.36
261.98
262.23
266.31
0.23
1.24
5.39
1.5
3.81
0.038
0.087
0.00046
0.046
0.022
0.71
0.33
0.44
0.035
0.27
2.74
1.63
0.15
0.21
0.81
0.00093
4.19
0.0042
52.5
284.3
1246.9
348.4
884.9
9.0
21.0
0.11
11.3
5.5
176.3
82.4
112.4
9.0
69.5
706.4
421.2
38.8
54.7
211.7
0.24
1098.7
1.1
3.59
0.66
4.32
5.81
0.7
3.16
3.27
0.021
0.0076
8.96
0.81
1.2
1.17
1.54
1.75
3.04
56
1.22
0.48
1.36
0.19
2.22
16.9
819.3
151.3
999.3
1349.4
162.6
749.9
789.1
5.1
1.9
2219.8
201.1
299.6
298.9
394.8
450.5
783.7
14470.4
315.3
124.9
355.4
49.8
582.2
4500.6
266.31
0.96
255.7
1.3
346.2
266.32
268.28
269.13
271.49
272.75
274.42
275.73
0.036
1.33
0.0083
0.015
0.0031
0.11
0.059
9.6
356.8
2.2
4.1
0.85
30.2
16.3
0.19
0.74
10
0.0037
0.41
0.0073
18.9
50.6
198.5
2691.3
1.0
111.8
2.0
5211.3
K-29
In Vitro Cytotoxicity Test Methods BRD Appendix K3
November 2006
RC IC50 and LD50 Values for RC Substances with Rat Oral LD50 Data
Substance
Triethyl citrate
Azathioprine
Amitriptyline
Imidazolidinyl urea
Dibutyl phthalate
Cyclophosphamide * H2O
Flufenamic acid
Cycloheximide
Diazepam
Retinol
Dihydralazine sulfate
Sodium dodecyl sulfate
Lindane
Parathion
Diphenhydramine * HCl
Naftipramide
Cis-platinum
all-trans-Retinoic acid
Captan
Chlorambucil
Orphenadrine * HCl
Buflomedil
Warfarin
Phenylbutazone
Aflatoxin B1
Refortan
Imipramine * HCl
p,p'-DDE
Chlorpromazine
p,p'-DDD
Chloramphenicol
Oxyphenbutazone
Tributyltin chloride
Malathion
Frusemide
Mitomycin C
Metamizol
Dicoumarol
Caffeine sodium benzoate
Papaverine
Molecular
Weight
(g/mole)
276.32
277.29
277.44
278.26
278.38
279.13
281.25
281.39
284.76
286.5
288.32
289.43
290.82
291.28
291.85
298.47
300.07
300.48
300.59
304.24
305.88
307.43
308.35
308.41
312.29
313.1
316.91
318.02
318.89
320.04
323.15
324.41
325.53
330.38
330.76
334.37
334.38
336.31
338.33
339.42
IC50x
(mM)1
14.7
0.14
0.056
0.36
0.76
3.12
0.029
0.00059
0.16
0.00054
0.14
0.27
0.41
0.093
0.24
0.084
0.0028
0.11
0.0039
0.076
0.49
1.35
0.67
0.32
0.034
0.25
0.054
0.1
0.014
0.024
0.79
0.19
0.00054
0.2
2.33
0.00084
0.58
0.027
5.67
0.045
K-30
IC50x
(mg/mL)1
Rat Oral
LD50
(mmol/kg)2
4061.9
38.8
15.5
100.2
211.6
870.9
8.2
0.17
45.6
0.15
40.4
78.1
119.2
27.1
70.0
25.1
0.84
33.1
1.2
23.1
149.9
415.0
206.6
98.7
10.6
78.3
17.1
31.8
4.5
7.7
255.3
61.6
0.18
66.1
770.7
0.28
193.9
9.1
1918.3
15.3
25.3
1.93
1.15
9.34
43.1
0.34
0.97
0.0071
2.49
6.98
2.84
4.45
0.26
0.0069
2.93
3.45
0.086
6.66
33.3
0.25
1.39
1.19
1.05
1.22
0.016
10.1
0.96
2.77
0.44
0.35
10.5
3.08
0.37
2.68
7.86
0.042
21.5
2.11
2.54
0.96
Rat Oral
LD50
(mg/kg)2
6990.9
535.2
319.1
2598.9
11998.2
94.9
272.8
2.0
709.1
1999.8
818.8
1288.0
75.6
2.0
855.1
1029.7
25.8
2001.2
10009.6
76.1
425.2
365.8
323.8
376.3
5.0
3162.3
304.2
880.9
140.3
112.0
3393.1
999.2
120.4
885.4
2599.8
14.0
7189.2
709.6
859.4
325.8
In Vitro Cytotoxicity Test Methods BRD Appendix K3
November 2006
RC IC50 and LD50 Values for RC Substances with Rat Oral LD50 Data
Substance
Diquat dibromide
Gibberellic acid
Dodecylbenzene sodiumsulfonate
Triisooctylamine
p,p'-DDT
Benzylpenicillin sodium
Indomethacin
Quinine * HCl
Cetyltrimethylammonium chloride
Hexadecyltrimethylammonium
bromide
Aldrin
Benzalkonium chloride
Triphenyltin hydroxide
Potassium hexacyanoferrate II
Amphetamine sulfate
Homatropine methylbromide
Kelthane
Di(2-ethylhexyl)adipate
Ioxynil
Heptachlor
Dextropropoxyphene * HCl
Dieldrin
Scopolamine * HBr
Di(2-ethylhexyl)phthalate
Rotenone
Hexachlorophene
Chlordan
Hydroxyzine * HCl
Chloroquine sulfate
Quinidine sulfate
Oxatomide
Xanthinol nicotinate
Mitoxantrone
Amethopterin
Dimenhydrinate
Emetine
Tetracycline * HCl
VerapamilHCl
Chlorhexidine
Molecular
Weight
(g/mole)
IC50x
(mM)1
IC50x
(mg/mL)1
Rat Oral
LD50
(mmol/kg)2
Rat Oral
LD50
(mg/kg)2
344.08
346.41
348.52
353.76
354.48
356.4
357.81
360.92
362.16
0.16
2.3
0.42
0.023
0.16
5.73
0.16
0.075
0.021
55.1
796.7
146.4
8.1
56.7
2042.2
57.2
27.1
7.6
0.67
18.2
3.62
4.58
0.32
19.4
0.034
1.72
1.31
230.5
6304.7
1261.6
1620.2
113.4
6914.2
12.2
620.8
474.4
364.53
0.0089
3.2
1.12
408.3
364.9
365
367.03
368.37
368.54
370.33
370.48
370.64
370.91
373.3
375.98
380.9
384.31
390.62
394.45
406.89
409.76
411.41
418
422.54
426.61
434.51
444.54
454.5
470.02
480.71
480.94
491.13
505.52
0.067
0.0052
0.000049
42.3
1.97
9
0.012
3.15
0.11
0.059
0.49
0.18
1.08
0.84
0.00013
0.0079
0.06
0.067
0.06
0.12
0.019
15.8
0.0024
0.00014
0.076
0.00016
0.14
0.1
0.015
24.4
1.9
0.0180
15582.1
726.0
3333.0
4.4
1167.5
40.8
22.0
184.2
68.6
415.1
328.1
0.051
3.2
24.6
27.6
25.1
50.7
8.1
6865.3
1.07
0.064
35.7
0.077
67.3
49.1
7.6
0.11
1.1
0.12
17.4
0.15
3.24
1.55
24.6
0.3
0.11
0.22
0.12
3.3
79.4
0.33
0.15
1.12
2.31
2.6
1.08
3.31
32.5
1.32
0.3
2.81
0.14
13.4
0.22
18.2
40.1
401.5
44.0
6409.6
55.3
1199.9
574.2
9117.7
111.3
41.1
82.7
45.7
1268.2
31015.2
130.2
61.0
458.9
950.4
1086.8
456.3
1412.1
14121.6
586.8
136.4
1320.8
67.3
6444.6
108.0
9200.5
K-31
In Vitro Cytotoxicity Test Methods BRD Appendix K3
November 2006
RC IC50 and LD50 Values for RC Substances with Rat Oral LD50 Data
Substance
Chloroquine diphosphate
Triton X-100
Atropine sulfate
Digitoxin
Trypan blue
Actinomycin D
Molecular
Weight
(g/mole)
515.92
647
676.9
765.05
964.88
1255.6
IC50x
(mM)1
0.017
0.055
0.22
0.00011
0.095
0.0000081
IC50x
(mg/mL)1
Rat Oral
LD50
(mmol/kg)2
8.8
35.6
148.9
0.0842
91.7
0.0102
1.88
2.78
0.92
0.073
6.43
0.0057
Rat Oral
LD50
(mg/kg)2
969.9
1798.7
622.7
55.8
6204.2
7.2
Abbreviations: RC=Registry of Cytotoxicity.
1
Geometric mean of the IC50 values collected from the literature for various in vitro basal cytotoxicity endpoints and cell
types (from the RC [Halle 1998, 2003]).
2
Rat oral LD50 values used in the RC (Halle 1998, 2003), which generally came from the 1983/1984 Registry of Toxic
Effects for Chemical Substances®.
K-32