APPENDIX A Registry of Cytotoxicity List of 347 Chemicals

APPENDIX A
Registry of Cytotoxicity
List of 347 Chemicals
(Sorted by IC50 and LD50 Values)
Appendix A: Registry of Cytotoxicity
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by IC50 (mM)
RC No
2
3
132
6
133
8
134
9
10
135
11
12
136
137
138
139
140
13
141
142
143
14
144
15
145
146
16
147
148
17
149
150
151
152
18
153
154
155
156
20
157
21
158
22
23
159
25
160
26
161
27
29
162
31
164
163
165
166
33
167
168
169
170
35
171
37
172
173
174
175
176
177
178
MEIC No Chemical
Actinomycin D
Aminopterin
Triphenyltin hydroxide
Colchicine
Cytochalasin D
Digitoxin
Rotenone
Amethopterin
Emetine
2,3,7,8-Tetrachlorodibenzo-p-dioxin
Doxorubicin * HCl
Puromycin
Diethyldithiocarbamate sodium* 3H20
Triethyltin chloride
Tributyltin chloride
Retinol
6-Thioguanine
Cycloheximide
Cytosine arabinoside
Methylmercury chloride
Triethylene melamine
Mitomycin C
Sodium bichromate VI
8-Azaguanine
Potassium chromate VI
Potassium bichromate VI
Azaserine
Mitoxantrone
Nitrogen mustard * HCl
5-Fluorouracil
Chromium VI trioxide
Cis-platinum
Hexachlorocyclopentadiene
8-Hydroxyquinoline
Captan
26 Arsenic III trioxide
Maneb
Benzalkonium chloride
Stearyltrimethylammonium chloride
Cadmium II chloride
38 Hexachlorophene
6-Mercaptopurine
Dichlorophene
6 Digoxin
Daraprim
Hexadecyltrimethylammonium bromide
Thio-TEPA
N-Methyl-N'-nitro-N-nitrosoguanidine
Kelthane
Silver I nitrate
Chlorpromazine
28 Mercury II chloride
Chlorhexidine
41 Chloroquine diphosphate
Oxatomide
Cetyltrimethylammonium chloride
Isoproterenol * HCl
Triisooctylamine
p-Chloromercuribenzoic acid
p,p'-DDD
Dicoumarol
Epinephrine bitartrate
29 Thioridazine * HCl
Flufenamic acid
Fumagillin
Aflatoxin B1
Nabam
39 Pentachlorophenol
Ambazone
Norepinephrine
Papaverine
Busulphan
Salicylanilide
A-1
IC 50x (mM)
0.0000081
0.000012
0.000049
0.000054
0.000092
0.00011
0.00013
0.00014
0.00016
0.0002
0.00033
0.00033
0.00039
0.00046
0.00054
0.00054
0.00057
0.00059
0.00068
0.00071
0.00078
0.00084
0.00093
0.0013
0.0015
0.002
0.002
0.0024
0.0026
0.0026
0.0027
0.0028
0.0031
0.0033
0.0039
0.0042
0.0042
0.0052
0.006
0.0064
0.0079
0.008
0.0083
0.0085
0.0089
0.0089
0.011
0.012
0.012
0.013
0.014
0.015
0.015
0.017
0.019
0.021
0.022
0.023
0.024
0.024
0.027
0.028
0.029
0.029
0.031
0.034
0.035
0.036
0.038
0.039
0.045
0.046
0.046
Oral Rat or
Mouse LD50
(mmol/kg)
0.0057
0.0068
0.12
0.015
0.071
0.073
0.33
0.3
0.14
0.00035
1.2
1.43
6.66
0.021
0.37
6.98
0.96
0.0071
12.9
0.23
0.005
0.042
0.19
9.86
0.93
0.65
0.98
1.32
0.052
1.77
0.8
0.086
0.41
8.27
33.3
0.1
16.9
1.1
1.54
0.48
0.15
1.84
10
0.023
0.51
1.12
0.2
0.61
1.55
0.29
0.44
0.0037
18.2
1.88
3.31
1.31
8.96
4.58
0.07
0.35
2.11
0.012
0.88
0.97
4.36
0.016
1.54
0.19
3.16
0.12
0.96
0.0076
11.3
Oral Rat or
Mouse LD50
(mg/kg)
7.2
3.0
44.0
6.0
36.0
55.8
130.2
136.4
67.3
0.1
696.0
674.4
1500.7
5.1
120.4
1999.8
160.5
2.0
3137.9
57.7
1.0
14.0
49.8
1500.1
180.6
191.2
169.7
586.8
10.0
230.3
80.0
25.8
111.8
1200.6
10009.6
19.8
4500.6
401.5
536.1
88.0
61.0
280.0
2691.3
18.0
126.9
408.3
37.8
89.7
574.2
49.3
140.3
1.0
9200.5
969.9
1412.1
474.4
2219.8
1620.2
25.0
112.0
709.6
4.0
358.2
272.8
1999.5
5.0
394.8
50.6
749.9
20.3
325.8
1.9
2409.7
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by IC50 (mM)
RC No MEIC No Chemical
Acrolein
179
p-Phenylenediamine
180
181
30 Thallium I sulfate
Imipramine * HCl
38
Triton X-100
182
2,4-Dichlorophenol
39
183
5 Amitriptyline
Butylated hydroxytoluene
184
Heptachlor
185
Zineb
186
Chlordan
40
Chloroquine sulfate
41
p-Aminophenol
42
4-Hexylresorcinol
187
Aldrin
43
Hydroxyzine * HCl
44
t-Butyl hydroquinone
188
Antimycin
189
Quinine * HCl
45
Chlorambucil
190
Dimenhydrinate
191
1,3-Bis(2-chloroethyl)- 1-nitrosourea
192
5-Azacytidine
193
Naftipramide
47
Mefenamic acid
48
Parathion
49
p-Toluylendiamine
194
Trypan blue
50
p,p'-DDA
195
196
40 VerapamilHCl
p,p'-DDE
197
Disulfoton
51
Ioxynil
198
Cupric chloride
199
all-trans-Retinoic acid
52
Dimethylaminoethyl methacrylate (polymer)
200
53
43 Quinidine sulfate
54
23 Propranolol * HCl
13-cis-Retinoic acid
201
Formaldehyde
202
Zinc II chloride
55
Manganese II chloride *4 H2O
56
L-Dopa
57
Thallium I acetate
203
Azathioprine
204
Dihydralazine sulfate
58
Tetracycline * HCl
59
Versalide
205
Indomethacin
60
p,p'-DDT
61
Cobalt II chloride
62
Diquat dibromide
206
63
4 Diazepam
Dieldrin
207
Bendiocarb
64
Undecylenic acid
208
Propylparaben
209
Oxyphenbutazone
65
p-Nitrophenol
210
67
15 Malathion
Catechol
211
2,4-Dinitrophenol
68
Secobarbital sodium
69
70
49 Atropine sulfate
p-Cresol
212
Ammonium persulfate
213
Thymol
214
Diphenhydramine * HCl
71
Chlorotetracycline
215
Butylated hydoxyanisole
72
Refortan
216
Carbaryl
73
Nickel II chloride
74
A-2
IC 50x (mM)
0.047
0.05
0.054
0.054
0.055
0.055
0.056
0.056
0.059
0.059
0.06
0.06
0.062
0.064
0.067
0.067
0.069
0.07
0.075
0.076
0.076
0.078
0.079
0.084
0.087
0.093
0.094
0.095
0.099
0.1
0.1
0.11
0.11
0.11
0.11
0.11
0.12
0.12
0.12
0.12
0.13
0.13
0.13
0.14
0.14
0.14
0.14
0.15
0.16
0.16
0.16
0.16
0.16
0.18
0.18
0.18
0.18
0.19
0.2
0.2
0.2
0.21
0.21
0.22
0.22
0.23
0.23
0.24
0.24
0.24
0.25
0.26
0.27
Oral Rat or
Mouse LD50
(mmol/kg)
0.82
0.74
0.057
0.96
2.78
3.56
1.15
4.04
0.11
18.9
1.12
2.6
15.2
2.83
0.11
2.31
4.81
0.45
1.72
0.25
2.81
0.093
2.34
3.45
3.27
0.0069
0.83
6.43
2.1
0.22
2.77
0.0073
0.3
1.04
6.66
11.1
1.08
1.59
11.3
26.6
2.57
7.5
9.03
0.13
1.93
2.84
13.4
1.22
0.034
0.32
0.62
0.67
2.49
0.12
0.8
13.6
35.1
3.08
2.52
2.68
35.3
0.16
0.48
0.92
1.91
3.59
6.52
2.93
5.22
12.2
10.1
1.24
0.81
Oral Rat or
Mouse LD50
(mg/kg)
46.0
80.0
28.8
304.2
1798.7
580.3
319.1
890.4
41.1
5211.3
458.9
1086.8
1658.9
549.9
40.1
950.4
799.6
112.6
620.8
76.1
1320.8
19.9
571.5
1029.7
789.1
2.0
101.4
6204.2
590.4
108.0
880.9
2.0
111.3
139.8
2001.2
1745.4
456.3
470.4
3395.4
798.8
350.2
1484.4
1780.8
34.2
535.2
818.8
6444.6
315.3
12.2
113.4
80.5
230.5
709.1
45.7
178.6
2506.6
6325.7
999.2
350.6
885.4
3887.2
29.5
124.9
622.7
206.6
819.3
979.6
855.1
2500.0
2199.3
3162.3
249.5
105.0
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by IC50 (mM)
RC No MEIC No Chemical
IC 50x (mM)
Trichlorfon
75
0.27
Sodium dodecyl sulfate
76
0.27
Amrinone
217
0.28
o-Phenylenediamine
218
0.31
6-Methylcoumarin
78
0.31
Phenylbutazone
79
0.32
2-Thiouracil
80
0.32
Hydralazine
219
0.33
81
27 Cupric sulfate * 5 H2O
0.33
Imidazolidinyl urea
238
0.36
m-Dinitrobenzene
220
0.39
82
44 Diphenylhydantoin
0.39
2-Nitro-p-phenylenediamine
221
0.39
Glibenclamide
222
0.4
223
32 Lindane
0.41
n-Butyl benzoate
224
0.41
Ammonium sulfide
225
0.42
Dodecylbenzene sodiumsulfonate
226
0.42
227
46 Sodium oxalate
0.44
2,4,5-Trichlorophenoxyacetic acid
228
0.44
229
22 Dextropropoxyphene * HCl
0.49
230
42 Orphenadrine * HCl
0.49
Tween 80
231
0.49
o-Cresol
232
0.52
Ibuprofen
233
0.52
Phenylthiourea
234
0.54
235
25 Paraquat
0.54
Thiopental
83
0.55
Amobarbital
84
0.56
Hydrogen peroxide 90%
236
0.56
Metamizol
85
0.58
Beryllium II sulfate
237
0.61
m-Cresol
239
0.66
Pentoxifylline
240
0.66
86
31 Warfarin
0.67
Sodium azide
241
0.71
Pentobarbital sodium
87
0.71
1,2,4-Trichlorobenzene
242
0.71
p-Anisidine
243
0.73
Doxylamine succinate
244
0.75
Dibutyl phthalate
88
0.76
89
16 2,4-Dichlorophenoxyacetic acid
0.77
Iproniazid
90
0.79
91
45 Chloramphenicol
0.79
Resorcinol
245
0.8
246
37 Barium II nitrate
0.81
(+)-Thalidomide
247
0.81
Di(2-ethylhexyl)phthalate
92
0.84
Sulfisoxazole
93
0.85
m-Aminophenol
248
0.86
Menthol
94
0.95
3-Cyano-2-morpholino-5-(pyrid-4-yl)-pyridine
(Chemical 122)
249
0.96
Valproate sodium
250
1
Scopolamine * HBr
251
1.08
Salicylamide
95
1.08
252
19 Potassium cyanide
1.12
Cygon
96
1.24
Phenacetin
97
1.27
Isoxepac
253
1.33
Buflomedil
254
1.35
Methylparaben
98
1.42
Sodium monochloroacetate
255
1.45
Nalidixic acid
99
1.5
Tin II chloride
256
1.51
Isononylaldehyde
257
1.52
L-Ascorbic acid
100
1.52
Glutethimide
101
1.56
Acrylamide
102
1.61
Diethyl sebacate
258
1.63
Methyl salicylate
259
1.7
Coumarin
260
1.71
103
18 Nicotine
1.79
A-3
Oral Rat or
Mouse LD50
(mmol/kg)
1.75
4.45
0.54
9.89
10.5
1.22
7.8
0.56
1.2
9.34
0.49
0.79
20.1
6.58
0.26
28.8
3.29
3.62
1.16
1.17
0.22
1.39
19.1
1.12
4.89
0.02
0.31
2.48
1.52
58.8
21.5
0.78
2.24
4.98
1.05
0.69
0.81
4.17
11.4
1.21
43.1
1.67
2.04
10.5
2.73
1.36
1.55
79.4
25.4
15.2
20.3
Oral Rat or
Mouse LD50
(mg/kg)
450.5
1288.0
101.1
1069.7
1681.9
376.3
999.6
89.7
299.6
2598.9
82.4
199.3
3078.5
3250.8
75.6
5133.6
168.2
1261.6
155.4
298.9
82.7
425.2
25021.0
121.1
1008.9
3.0
57.7
601.1
344.0
2000.4
7189.2
82.0
242.3
1386.2
323.8
44.9
201.1
756.6
1404.1
470.1
11998.2
369.1
365.7
3393.1
300.6
355.4
400.3
31015.2
6790.2
1658.9
3172.9
1.3
10.2
3.3
13.8
0.15
0.66
9.21
0.74
1.19
11.5
0.65
5.81
3.69
22.8
67.6
2.76
2.39
56
5.83
2
0.31
346.2
1695.4
1268.2
1892.7
9.8
151.3
1650.8
198.5
365.8
1749.8
75.7
1349.4
699.6
3243.8
11907.1
599.7
169.9
14470.4
887.1
292.3
50.3
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by IC50 (mM)
RC No MEIC No Chemical
Tolbutamide
104
105
21 Theophylline
261
3 Ferrous sulfate
106
14 Sodium I fluoride
262
47 Amphetamine sulfate
107
2 Acetylsalicylic acid
Gibberellic acid
108
Frusemide
109
Acrylonitrile
110
Acetaldehyde
263
Clofibric acid
111
112
48 Caffeine
Chloral hydrate
264
113
1 Acetaminophen
Streptomycin sulfate
265
Natulan * HCl
114
Potassium hexacyanoferrate III
266
p-Hydroxybenzoic acid
267
115
12 Phenol
1-Octanol
268
Cyclophosphamide * H2O
116
Potassium I fluoride
269
Di(2-ethylhexyl)adipate
117
Propionaldehyde
270
Styrene
271
Salicylic acid
272
Bromobenzene
273
L-Cysteine
274
Nitrilotriacetic acid
275
Ambuphylline
276
118
24 Phenobarbital
Potassium cyanate
277
Phenylephrine * HCl
278
Thioacetamide
279
Theophylline sodium acetate
280
1,2-Dibromomethane
281
Sodium salicylate
119
(-)-Phenylephrine
282
Milrinone
283
5-Aminosalicylic acid
120
Aminophenazone
121
Ammonium chloride
284
Diethyl phthalate
122
Caffeine sodium benzoate
285
Benzylpenicillin sodium
286
Benzylalcohol
287
1-Heptanol
288
Tetrachloroethene
289
Sodium sulfite
290
Aniline
291
Allylalcohol
292
Diisopropylamine dichloroacetate
293
123
35 Isoniazid
Trichloroacetic acid
294
2,5-Hexanedione
295
Acetazolamide
124
125
34 Carbon tetrachloride
Homatropine methylbromide
296
297
11 1,1,1-Trichloroethane
Dichloroacetic acid
298
Imidazole
299
Antipyrine
300
301
17 Xylene
Nitrobenzene
302
Theophylline sodium
303
Calcium II chloride
304
n-Butanal
305
Anisole
306
2-Ethylbutanal
307
308
33 Chloroform
Isobutanal
309
Triethyl citrate
126
Tributylamine
310
IC 50x (mM)
1.81
1.83
1.85
1.85
1.97
2.27
2.3
2.33
2.42
2.45
2.61
2.64
2.65
2.71
2.73
2.74
2.82
2.92
3.01
3.06
3.12
3.13
3.15
3.25
3.3
3.38
3.46
3.56
3.61
3.67
3.81
4.14
4.16
4.17
4.19
4.2
4.33
4.45
4.77
5.07
5.39
5.52
5.52
5.67
5.73
5.81
6.25
6.54
6.78
6.9
6.94
7
7.49
8.19
8.45
8.49
8.51
9
10.3
11.5
11.5
11.6
12
12.2
12.4
12.4
12.8
13.2
13.2
13.4
13.5
14.7
15.4
A-4
Oral Rat or
Mouse LD50
(mmol/kg)
9.62
3.33
2.1
4.29
0.15
5.55
18.2
7.86
1.54
43.8
5.82
0.99
2.9
15.9
0.34
3.04
9.02
15.9
4.4
13.7
0.34
4.22
24.6
24.3
48
6.45
17.2
5.45
7.69
2.23
0.7
10.4
1.72
4.01
2.22
0.62
9.99
2.09
0.43
50.6
4.32
30.8
38.7
2.54
19.4
11.4
28
53.4
6.51
4.72
1.1
7.39
4.74
30.6
23.7
19.3
18.2
3.24
77.2
21.9
27.6
9.56
40.5
5.2
2.19
9.01
34.5
34.2
39.7
7.61
39
25.3
2.91
Oral Rat or
Mouse LD50
(mg/kg)
2601.1
600.0
319.0
180.1
55.3
999.9
6304.7
2599.8
81.7
1929.8
1249.3
192.3
479.7
2403.8
495.6
783.7
2970.0
2196.3
414.1
1784.6
94.9
245.2
9117.7
1411.6
4999.7
890.9
2700.7
660.4
1470.0
600.7
162.6
843.6
350.3
301.3
582.2
107.8
1599.5
349.5
90.8
7749.4
999.3
1647.8
8601.5
859.4
6914.2
1232.9
3254.4
8854.8
820.5
439.6
63.9
1700.9
650.1
4999.4
2705.6
4289.6
2799.3
1199.9
10298.5
2823.8
1879.3
1799.7
4300.3
640.2
445.0
999.9
2488.1
3698.7
3977.1
908.4
2812.7
6990.9
539.5
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by IC50 (mM)
RC No MEIC No Chemical
1-Hexanol
311
Benzoic acid
312
Xanthinol nicotinate
313
Saccharin
314
Isobenzoic furano dione
315
Toluene
316
Barbital sodium
317
Trifluoroacetic acid
318
Dimethyl phthalate
127
Methylpentinol
319
N,N-Dimethylacetamide
320
Acetic acid
321
1-Pentanol
322
Urethan
323
2-Butoxyethanol
324
Cyclohexanol
325
Halothane
326
327
20 Lithium I sulfate
328
36 Dichloromethane
Sodium cyclamate
329
Sulfuric acid
330
Strontium II chloride
331
1,4-Dioxane
332
Lithium I chloride
333
Isobutanol
334
Potassium hexacyanoferrate II
335
Nicotinamide
336
Pyridine
337
1-Butanol
338
1-Nitropropane
339
Diethylene glycol
340
Lactic acid
341
Piperazine
342
Magnesium II chloride * 6 H2O
343
344
13 Sodium chloride
Sodium I bromide
345
346
50 Potassium I chloride
Thiourea
347
1-Propanol
348
Ethyl methyl ketone
349
Tetrahydrofurfuryl alcohol
350
Dimethylformamide
351
1,2,6-Hexanetriol
352
Ethyl acetate
353
128
10 2-Propanol
1,3,5-Trioxane
354
D-Glucose
355
2-Methoxyethanol
356
Dimethyl sulfoxide
129
Propylene glycol
357
Acetonitrile
358
130
9 Ethanol
Acetone
359
360
7 Ethylene glycol
Glycerol
131
361
8 Methanol
IC 50x (mM)
15.4
15.7
15.8
16.4
17
17.1
18.6
20.5
23.4
23.8
24.2
24.3
24.9
25.9
26
26.3
31.1
33.7
34.9
35.4
36
36.4
38.1
38.6
40.1
42.3
44.4
46.9
52.5
57.9
62.1
66
67.2
70.4
75.9
77.4
82
86
96.5
104
111
114
123
128
167
213
226
251
252
342
368
379
444
555
624
930
Oral Rat or
Mouse LD50
(mmol/kg)
7.04
20.7
32.5
92.8
27.1
54.3
3.88
1.75
35.5
5.35
58.4
55.1
34.4
28.1
12.5
20.6
28.8
10.8
18.8
75.8
21.8
14.2
47.7
17.9
33.2
17.4
28.7
11.3
10.7
5.11
139
41.4
22.1
39.8
51.3
33.4
34.9
1.64
89.8
47.1
24.5
38.3
119
125
97.2
8.88
143
32.3
252
263
92.5
304
168
138
137
406
Oral Rat or
Mouse LD50
(mg/kg)
719.5
2528.1
14121.6
17000.0
4014.1
5003.7
800.1
199.6
6894.1
525.2
5089.0
3309.3
3033.0
2504.0
1477.5
2063.7
5684.8
1187.4
1596.7
15254.0
2138.1
2251.0
4203.3
758.8
2461.4
6409.6
3505.4
893.9
793.3
455.4
14753.5
3729.7
1904.1
8092.5
2998.0
3504.3
2601.8
124.9
5397.9
3396.9
2502.7
2800.1
15969.8
11015.0
5842.7
800.0
25765.7
2458.4
19691.3
20016.9
3798.1
14008.3
9759.1
8567.0
12619.1
13012.3
Reference
Halle, W. 1998. Toxizitätsprüfungen in Zellkulturen für eine Vorhersage der akuten Toxizität (LD50) zur Einsparung
von Tierversuchen. Life Sciences/Lebenswissenschaften, Volume 1, 94 pp., Jülich: Forschungszentrum Jülich.
A-5
Appendix A: Registry of Cytotoxicity
A-6
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by LD50 (mg/kg)
RC No MEIC No Chemical
2,3,7,8-Tetrachlorodibenzo-p-dioxin
135
29
28 Mercury II chloride
Triethylene melamine
143
Busulphan
177
Cycloheximide
13
Disulfoton
51
Parathion
49
Aminopterin
3
Phenylthiourea
234
Epinephrine bitartrate
169
Aflatoxin B1
37
Triethyltin chloride
137
Colchicine
6
Actinomycin D
2
252
19 Potassium cyanide
Nitrogen mustard * HCl
148
Indomethacin
60
Mitomycin C
14
22
6 Digoxin
153
26 Arsenic III trioxide
1,3-Bis(2-chloroethyl)- 1-nitrosourea
192
Norepinephrine
175
p-Chloromercuribenzoic acid
33
Cis-platinum
150
181
30 Thallium I sulfate
2,4-Dinitrophenol
68
Thallium I acetate
203
Cytochalasin D
133
Thio-TEPA
25
Aldrin
43
Heptachlor
185
Triphenyltin hydroxide
132
Sodium azide
241
Dieldrin
207
Acrolein
179
Silver I nitrate
161
Sodium bichromate VI
144
103
18 Nicotine
173
39 Pentachlorophenol
262
47 Amphetamine sulfate
Digitoxin
8
235
25 Paraquat
Methylmercury chloride
142
157
38 Hexachlorophene
Allylalcohol
292
Emetine
10
223
32 Lindane
Sodium monochloroacetate
255
Chlorambucil
190
Chromium VI trioxide
149
p-Phenylenediamine
180
Cobalt II chloride
62
Acrylonitrile
110
Beryllium II sulfate
237
m-Dinitrobenzene
220
229
22 Dextropropoxyphene * HCl
Cadmium II chloride
20
Hydralazine
219
N-Methyl-N'-nitro-N-nitrosoguanidine
160
Milrinone
283
Cyclophosphamide * H2O
116
Amrinone
217
p-Toluylendiamine
194
Nickel II chloride
74
1,2-Dibromomethane
281
196
40 VerapamilHCl
Ioxynil
198
Hexachlorocyclopentadiene
151
p,p'-DDD
167
Antimycin
189
p,p'-DDT
61
Tributyltin chloride
138
o-Cresol
232
A-7
IC 50x (mM)
0.0002
0.015
0.00078
0.046
0.00059
0.11
0.093
0.000012
0.54
0.028
0.034
0.00046
0.000054
0.0000081
1.12
0.0026
0.16
0.00084
0.0085
0.0042
0.078
0.039
0.024
0.0028
0.054
0.21
0.14
0.000092
0.011
0.067
0.059
0.000049
0.71
0.18
0.047
0.013
0.00093
1.79
0.036
1.97
0.00011
0.54
0.00071
0.0079
6.94
0.00016
0.41
1.45
0.076
0.0027
0.05
0.16
2.42
0.61
0.39
0.49
0.0064
0.33
0.012
4.77
3.12
0.28
0.094
0.27
4.2
0.1
0.11
0.0031
0.024
0.07
0.16
0.00054
0.52
Oral Rat or
Mouse LD50
(mmol/kg)
0.00035
0.0037
0.005
0.0076
0.0071
0.0073
0.0069
0.0068
0.02
0.012
0.016
0.021
0.015
0.0057
0.15
0.052
0.034
0.042
0.023
0.1
0.093
0.12
0.07
0.086
0.057
0.16
0.13
0.071
0.2
0.11
0.11
0.12
0.69
0.12
0.82
0.29
0.19
0.31
0.19
0.15
0.073
0.31
0.23
0.15
1.1
0.14
0.26
0.65
0.25
0.8
0.74
0.62
1.54
0.78
0.49
0.22
0.48
0.56
0.61
0.43
0.34
0.54
0.83
0.81
0.62
0.22
0.3
0.41
0.35
0.45
0.32
0.37
1.12
Oral Rat or
Mouse LD50
(mg/kg)
0.1
1.0
1.0
1.9
2.0
2.0
2.0
3.0
3.0
4.0
5.0
5.1
6.0
7.2
9.8
10.0
12.2
14.0
18.0
19.8
19.9
20.3
25.0
25.8
28.8
29.5
34.2
36.0
37.8
40.1
41.1
44.0
44.9
45.7
46.0
49.3
49.8
50.3
50.6
55.3
55.8
57.7
57.7
61.0
63.9
67.3
75.6
75.7
76.1
80.0
80.0
80.5
81.7
82.0
82.4
82.7
88.0
89.7
89.7
90.8
94.9
101.1
101.4
105.0
107.8
108.0
111.3
111.8
112.0
112.6
113.4
120.4
121.1
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by LD50 (mg/kg)
RC No MEIC No Chemical
IC 50x (mM)
Thiourea
347
86
Secobarbital sodium
69
0.21
Daraprim
23
0.0089
Rotenone
134
0.00013
Amethopterin
9
0.00014
Cupric chloride
199
0.11
Chlorpromazine
27
0.014
Cygon
96
1.24
227
46 Sodium oxalate
0.44
6-Thioguanine
140
0.00057
118
24 Phenobarbital
3.81
Ammonium sulfide
225
0.42
Azaserine
16
0.002
Acrylamide
102
1.61
Bendiocarb
64
0.18
106
14 Sodium I fluoride
1.85
Potassium chromate VI
145
0.0015
Potassium bichromate VI
146
0.002
112
48 Caffeine
2.64
Isoxepac
253
1.33
82
44 Diphenylhydantoin
0.39
Trifluoroacetic acid
318
20.5
Pentobarbital sodium
87
0.71
p-Cresol
212
0.22
5-Fluorouracil
17
0.0026
Diquat dibromide
206
0.16
m-Cresol
239
0.66
Potassium I fluoride
269
3.13
Carbaryl
73
0.26
Flufenamic acid
35
0.029
6-Mercaptopurine
21
0.008
Coumarin
260
1.71
2,4,5-Trichlorophenoxyacetic acid
228
0.44
81
27 Cupric sulfate * 5 H2O
0.33
Resorcinol
245
0.8
Thioacetamide
279
4.17
Imipramine * HCl
38
0.054
Versalide
205
0.15
261
3 Ferrous sulfate
1.85
183
5 Amitriptyline
0.056
86
31 Warfarin
0.67
Papaverine
176
0.045
Amobarbital
84
0.56
3-Cyano-2-morpholino-5-(pyrid-4-yl)-pyridine
(Chemical 122)
249
0.96
(-)-Phenylephrine
282
4.45
Zinc II chloride
55
0.13
Phenylephrine * HCl
278
4.16
p-Nitrophenol
210
0.2
246
37 Barium II nitrate
0.81
170
29 Thioridazine * HCl
0.029
Iproniazid
90
0.79
Buflomedil
254
1.35
89
16 2,4-Dichlorophenoxyacetic acid
0.77
Phenylbutazone
79
0.32
Nabam
172
0.035
(+)-Thalidomide
247
0.81
Benzalkonium chloride
155
0.0052
Hexadecyltrimethylammonium bromide
159
0.0089
115
12 Phenol
3.01
230
42 Orphenadrine * HCl
0.49
Aniline
291
6.9
Theophylline sodium
303
12.4
Trichlorfon
75
0.27
1-Nitropropane
339
57.9
53
43 Quinidine sulfate
0.12
Chlordan
40
0.06
Doxylamine succinate
244
0.75
54
23 Propranolol * HCl
0.12
Cetyltrimethylammonium chloride
163
0.021
Chloral hydrate
264
2.65
Streptomycin sulfate
265
2.73
Methylpentinol
319
23.8
A-8
Oral Rat or
Mouse LD50
(mmol/kg)
1.64
0.48
0.51
0.33
0.3
1.04
0.44
0.66
1.16
0.96
0.7
3.29
0.98
2.39
0.8
4.29
0.93
0.65
0.99
0.74
0.79
1.75
0.81
1.91
1.77
0.67
2.24
4.22
1.24
0.97
1.84
2
1.17
1.2
2.73
4.01
0.96
1.22
2.1
1.15
1.05
0.96
1.52
Oral Rat or
Mouse LD50
(mg/kg)
124.9
124.9
126.9
130.2
136.4
139.8
140.3
151.3
155.4
160.5
162.6
168.2
169.7
169.9
178.6
180.1
180.6
191.2
192.3
198.5
199.3
199.6
201.1
206.6
230.3
230.5
242.3
245.2
249.5
272.8
280.0
292.3
298.9
299.6
300.6
301.3
304.2
315.3
319.0
319.1
323.8
325.8
344.0
1.3
2.09
2.57
1.72
2.52
1.36
0.88
2.04
1.19
1.67
1.22
1.54
1.55
1.1
1.12
4.4
1.39
4.72
2.19
1.75
5.11
1.08
1.12
1.21
1.59
1.31
2.9
0.34
5.35
346.2
349.5
350.2
350.3
350.6
355.4
358.2
365.7
365.8
369.1
376.3
394.8
400.3
401.5
408.3
414.1
425.2
439.6
445.0
450.5
455.4
456.3
458.9
470.1
470.4
474.4
479.7
495.6
525.2
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by LD50 (mg/kg)
RC No MEIC No Chemical
Azathioprine
204
Stearyltrimethylammonium chloride
156
Tributylamine
310
4-Hexylresorcinol
187
5-Azacytidine
193
Kelthane
26
2,4-Dichlorophenol
39
Theophylline sodium acetate
280
Mitoxantrone
147
p,p'-DDA
195
Glutethimide
101
105
21 Theophylline
Ambuphylline
276
Thiopental
83
Quinine * HCl
45
70
49 Atropine sulfate
Nitrobenzene
302
123
35 Isoniazid
L-Cysteine
274
Puromycin
12
Doxorubicin * HCl
11
Tin II chloride
256
63
4 Diazepam
Dicoumarol
168
1-Hexanol
311
Ambazone
174
1,2,4-Trichlorobenzene
242
Lithium I chloride
333
Natulan * HCl
114
Mefenamic acid
48
1-Butanol
338
Formaldehyde
202
t-Butyl hydroquinone
188
1,3,5-Trioxane
354
Barbital sodium
317
Dihydralazine sulfate
58
Ammonium persulfate
213
Sodium sulfite
290
Potassium cyanate
277
Diphenhydramine * HCl
71
Caffeine sodium benzoate
285
p,p'-DDE
197
67
15 Malathion
Methyl salicylate
259
Butylated hydroxytoluene
184
Salicylic acid
272
Pyridine
337
308
33 Chloroform
Hydroxyzine * HCl
44
31
41 Chloroquine diphosphate
Thymol
214
Oxyphenbutazone
65
Aminophenazone
121
2-Thiouracil
80
Calcium II chloride
304
107
2 Acetylsalicylic acid
Ibuprofen
233
Naftipramide
47
o-Phenylenediamine
218
Chloroquine sulfate
41
327
20 Lithium I sulfate
Homatropine methylbromide
296
8-Hydroxyquinoline
152
Benzylalcohol
287
Clofibric acid
111
Dodecylbenzene sodiumsulfonate
226
Scopolamine * HBr
251
Sodium dodecyl sulfate
76
Dimenhydrinate
191
Nalidixic acid
99
Pentoxifylline
240
p-Anisidine
243
Propionaldehyde
270
A-9
IC 50x (mM)
0.14
0.006
15.4
0.064
0.079
0.012
0.055
4.19
0.0024
0.099
1.56
1.83
3.67
0.55
0.075
0.22
12.2
7.49
3.56
0.00033
0.00033
1.51
0.16
0.027
15.4
0.038
0.71
38.6
2.74
0.087
52.5
0.12
0.069
213
18.6
0.14
0.23
6.78
4.14
0.24
5.67
0.1
0.2
1.7
0.056
3.38
46.9
13.4
0.067
0.017
0.23
0.19
5.39
0.32
12.4
2.27
0.52
0.084
0.31
0.06
33.7
9
0.0033
5.81
2.61
0.42
1.08
0.27
0.076
1.5
0.66
0.73
3.25
Oral Rat or
Mouse LD50
(mmol/kg)
1.93
1.54
2.91
2.83
2.34
1.55
3.56
2.22
1.32
2.1
2.76
3.33
2.23
2.48
1.72
0.92
5.2
4.74
5.45
1.43
1.2
3.69
2.49
2.11
7.04
3.16
4.17
17.9
3.04
3.27
10.7
26.6
4.81
8.88
3.88
2.84
3.59
6.51
10.4
2.93
2.54
2.77
2.68
5.83
4.04
6.45
11.3
7.61
2.31
1.88
6.52
3.08
4.32
7.8
9.01
5.55
4.89
3.45
9.89
2.6
10.8
3.24
8.27
11.4
5.82
3.62
3.3
4.45
2.81
5.81
4.98
11.4
24.3
Oral Rat or
Mouse LD50
(mg/kg)
535.2
536.1
539.5
549.9
571.5
574.2
580.3
582.2
586.8
590.4
599.7
600.0
600.7
601.1
620.8
622.7
640.2
650.1
660.4
674.4
696.0
699.6
709.1
709.6
719.5
749.9
756.6
758.8
783.7
789.1
793.3
798.8
799.6
800.0
800.1
818.8
819.3
820.5
843.6
855.1
859.4
880.9
885.4
887.1
890.4
890.9
893.9
908.4
950.4
969.9
979.6
999.2
999.3
999.6
999.9
999.9
1008.9
1029.7
1069.7
1086.8
1187.4
1199.9
1200.6
1232.9
1249.3
1261.6
1268.2
1288.0
1320.8
1349.4
1386.2
1404.1
1411.6
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by LD50 (mg/kg)
RC No MEIC No Chemical
Oxatomide
164
Nitrilotriacetic acid
275
2-Butoxyethanol
324
Manganese II chloride *4 H2O
56
8-Azaguanine
15
Diethyldithiocarbamate sodium* 3H20
136
328
36 Dichloromethane
Sodium salicylate
119
Triisooctylamine
166
Ammonium chloride
284
Phenacetin
97
p-Aminophenol
42
m-Aminophenol
248
6-Methylcoumarin
78
Valproate sodium
250
Diisopropylamine dichloroacetate
293
Dimethylaminoethyl methacrylate (polymer)
200
Methylparaben
98
L-Dopa
57
1-Octanol
268
Triton X-100
182
Antipyrine
300
Imidazole
299
Salicylamide
95
Piperazine
342
Acetaldehyde
263
Fumagillin
171
Retinol
139
Hydrogen peroxide 90%
236
all-trans-Retinoic acid
52
Cyclohexanol
325
Sulfuric acid
330
p-Hydroxybenzoic acid
267
Butylated hydoxyanisole
72
Isoproterenol * HCl
165
Strontium II chloride
331
113
1 Acetaminophen
Salicylanilide
178
2-Methoxyethanol
356
Isobutanol
334
n-Butanal
305
Chlorotetracycline
215
Tetrahydrofurfuryl alcohol
350
Urethan
323
Undecylenic acid
208
Benzoic acid
312
Imidazolidinyl urea
238
Frusemide
109
Tolbutamide
104
346
50 Potassium I chloride
Dichlorophene
158
Bromobenzene
273
2,5-Hexanedione
295
125
34 Carbon tetrachloride
Dimethylformamide
351
Isobutanal
309
Dichloroacetic acid
298
Potassium hexacyanoferrate III
266
344
13 Sodium chloride
1-Pentanol
322
2-Nitro-p-phenylenediamine
221
Cytosine arabinoside
141
Refortan
216
Menthol
94
Isononylaldehyde
257
Glibenclamide
222
1-Heptanol
288
Acetic acid
321
91
45 Chloramphenicol
13-cis-Retinoic acid
201
Ethyl methyl ketone
349
Sodium I bromide
345
Nicotinamide
336
A-10
IC 50x (mM)
0.019
3.61
26
0.13
0.0013
0.00039
34.9
4.33
0.023
5.52
1.27
0.062
0.86
0.31
1
7
0.11
1.42
0.13
3.06
0.055
11.6
11.5
1.08
67.2
2.45
0.031
0.00054
0.56
0.11
26.3
36
2.92
0.24
0.022
36.4
2.71
0.046
251
40.1
12.8
0.24
111
25.9
0.18
15.7
0.36
2.33
1.81
82
0.0083
3.46
8.45
8.51
114
13.5
11.5
2.82
75.9
24.9
0.39
0.00068
0.25
0.95
1.52
0.4
6.25
24.3
0.79
0.12
104
77.4
44.4
Oral Rat or
Mouse LD50
(mmol/kg)
3.31
7.69
12.5
7.5
9.86
6.66
18.8
9.99
4.58
30.8
9.21
15.2
15.2
10.5
10.2
7.39
11.1
11.5
9.03
13.7
2.78
9.56
27.6
13.8
22.1
43.8
4.36
6.98
58.8
6.66
20.6
21.8
15.9
12.2
8.96
14.2
15.9
11.3
32.3
33.2
34.5
5.22
24.5
28.1
13.6
20.7
9.34
7.86
9.62
34.9
10
17.2
23.7
18.2
38.3
39
21.9
9.02
51.3
34.4
20.1
12.9
10.1
20.3
22.8
6.58
28
55.1
10.5
11.3
47.1
33.4
28.7
Oral Rat or
Mouse LD50
(mg/kg)
1412.1
1470.0
1477.5
1484.4
1500.1
1500.7
1596.7
1599.5
1620.2
1647.8
1650.8
1658.9
1658.9
1681.9
1695.4
1700.9
1745.4
1749.8
1780.8
1784.6
1798.7
1799.7
1879.3
1892.7
1904.1
1929.8
1999.5
1999.8
2000.4
2001.2
2063.7
2138.1
2196.3
2199.3
2219.8
2251.0
2403.8
2409.7
2458.4
2461.4
2488.1
2500.0
2502.7
2504.0
2506.6
2528.1
2598.9
2599.8
2601.1
2601.8
2691.3
2700.7
2705.6
2799.3
2800.1
2812.7
2823.8
2970.0
2998.0
3033.0
3078.5
3137.9
3162.3
3172.9
3243.8
3250.8
3254.4
3309.3
3393.1
3395.4
3396.9
3504.3
3505.4
Appendix A
Registry of Cytotoxicity: List of 347 Chemicals Sorted by LD50 (mg/kg)
RC No MEIC No Chemical
Anisole
306
Lactic acid
341
Acetonitrile
358
Catechol
211
2-Ethylbutanal
307
Isobenzoic furano dione
315
1,4-Dioxane
332
Acetazolamide
124
301
17 Xylene
Maneb
154
Trichloroacetic acid
294
Styrene
271
Toluene
316
N,N-Dimethylacetamide
320
n-Butyl benzoate
224
Zineb
186
1-Propanol
348
Halothane
326
128
10 2-Propanol
Trypan blue
50
Gibberellic acid
108
Propylparaben
209
Potassium hexacyanoferrate II
335
Tetracycline * HCl
59
Sulfisoxazole
93
Dimethyl phthalate
127
Benzylpenicillin sodium
286
Triethyl citrate
126
Metamizol
85
5-Aminosalicylic acid
120
Magnesium II chloride * 6 H2O
343
360
7 Ethylene glycol
Diethyl phthalate
122
Tetrachloroethene
289
Di(2-ethylhexyl)adipate
117
Chlorhexidine
162
Acetone
359
Captan
18
297
11 1,1,1-Trichloroethane
Ethyl acetate
353
L-Ascorbic acid
100
Dibutyl phthalate
88
Glycerol
131
361
8 Methanol
130
9 Ethanol
Xanthinol nicotinate
313
Diethyl sebacate
258
Diethylene glycol
340
Sodium cyclamate
329
1,2,6-Hexanetriol
352
Saccharin
314
Dimethyl sulfoxide
129
Propylene glycol
357
Tween 80
231
D-Glucose
355
Di(2-ethylhexyl)phthalate
92
IC 50x (mM)
13.2
66
368
0.2
13.2
17
38.1
8.49
12
0.0042
8.19
3.3
17.1
24.2
0.41
0.059
96.5
31.1
167
0.095
2.3
0.18
42.3
0.14
0.85
23.4
5.73
14.7
0.58
5.07
70.4
555
5.52
6.54
3.15
0.015
444
0.0039
10.3
128
1.52
0.76
624
930
379
15.8
1.63
62.1
35.4
123
16.4
252
342
0.49
226
0.84
Oral Rat or
Mouse LD50
(mmol/kg)
34.2
41.4
92.5
35.3
39.7
27.1
47.7
19.3
40.5
16.9
30.6
48
54.3
58.4
28.8
18.9
89.8
28.8
97.2
6.43
18.2
35.1
17.4
13.4
25.4
35.5
19.4
25.3
21.5
50.6
39.8
138
38.7
53.4
24.6
18.2
168
33.3
77.2
125
67.6
43.1
137
406
304
32.5
56
139
75.8
119
92.8
252
263
19.1
143
79.4
Oral Rat or
Mouse LD50
(mg/kg)
3698.7
3729.7
3798.1
3887.2
3977.1
4014.1
4203.3
4289.6
4300.3
4500.6
4999.4
4999.7
5003.7
5089.0
5133.6
5211.3
5397.9
5684.8
5842.7
6204.2
6304.7
6325.7
6409.6
6444.6
6790.2
6894.1
6914.2
6990.9
7189.2
7749.4
8092.5
8567.0
8601.5
8854.8
9117.7
9200.5
9759.1
10009.6
10298.5
11015.0
11907.1
11998.2
12619.1
13012.3
14008.3
14121.6
14470.4
14753.5
15254.0
15969.8
17000.0
19691.3
20016.9
25021.0
25765.7
31015.2
Reference
Halle, W. 1998. Toxizitätsprüfungen in Zellkulturen für eine Vorhersage der akuten Toxizität (LD50) zur Einsparung
von Tierversuchen. Life Sciences/Lebenswissenschaften, Volume 1, 94 pp., Jülich: Forschungszentrum Jülich.
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