LAMPIRAN 1 Perhitungan Dosis Ekstrak Air Daun Stroberi (EADS)

LAMPIRAN 1
Perhitungan Dosis Ekstrak Air Daun Stroberi (EADS)
Prosedur pembuatan ekstrak air daun stroberi dilakukan di Sekolah Ilmu &
Teknologi Hayati ITB:
1. 500 gram daun stroberi kering ditumbuk menggunakan mortir dan stemper
hingga halus.
2. 300 gram simplisia dimaserasi dengan air suling sebanyak tiga kali (3 liter,
2 liter, dan 2 liter) selama 24 jam.
3. Filtrat disaring dan dipekatkan dengan evaporator pada suhu 70⁰C sampai
ekstrak mengental.
Rendemen ekstrak:
Berat botol
= 83, 3158 gram
Berat botol dan ekstrak
= 177, 6373 gram
Berat ekstrak
= 94,3215 gram
Dosis EADS tikus: 0,06 mg/100 cc, 0,6 mg/100 cc, 6 mg/100 cc, 60 mg/100 cc
(Mudnic, et al., 2009)
Diambil dosis 60 mg/ 100 cc
Rata-rata berat tikus = 200 gram
Volume lambung tikus = 2 cc
Dosis yang digunakan pada tikus:
Faktor konversi tikus 200 gram menjadi mencit 20 gram = 0,14 (Paget &
Barnes,1964)
Dosis yang digunakan pada mencit (20 gram):
53
54
Dosis dalam mg/kgBB dihitung dari EADS 3:
EADS 3 =
Jadi dosis yang digunakan:
EADS 1 = 2,1 mg/kgBB
EADS 2 = 4,2 mg/kgBB
EADS 3 = 8,4 mg/kgBB
Cara pembuatan dimulai dari EADS 3:
EADS 1 = 0,042 mg/0,5cc
EADS 2 = 0,084 mg/0,5cc
EADS 3 = 0,168 mg/0,5 cc
Karena kadar dosis terlalu kecil dan keterbatasan dalam penimbangan, maka
diambil dosis tikus 134mg/100cc (Mudnic, et. al., 2009):
Faktor konversi tikus 200 gram menjadi mencit 20 gram = 0,14 (Paget &
Barnes,1964)
Dosis yang digunakan pada mencit (20 gram):
Dosis dalam mg/kgBB dihitung dari EADS 3:
EADS 3 =
Jadi dosis yang digunakan:
EADS 1 = 4,69 mg/kgBB
EADS 2 = 9,38 mg/kgBB
EADS 3 = 18,75 mg/kgBB
55
Cara Pembuatan dimulai dari EADS 3:
EADS 1 = 0,094 mg/0,5cc
EADS 2 = 0,188 mg/0,5cc
EADS 3 = 0,375 mg/0,5 cc
56
LAMPIRAN 2
Perhitungan Dosis Sildenafil Sitrat
Kandungan sildenafil sitrat (1 tablet)
= 50 mg
Dosis efektif sildenafil sitrat untuk mencit = 5 mg/kgBB mencit (Tajuddin, 2005)
Berat badan mencit yang digunakan = 20 gram = 0,020 kg
Volume lambung mencit
= 0,5 cc
Perhitungan dosis yang digunakan
= 5mg
0,020
= 0,1 mg untuk mencit 20 gram
= 0,1 mg/0,5 cc
Perhitungan dosis CMC 1% yang digunakan:
57
LAMPIRAN 3
Data Kasar Penelitian
Hari Ketiga
Dosis1
Mencit
15 menit pertama
Introducing
Mounting
15 menit kedua
Introducing
Mounting
Jumlah
Introducing
Rerata
Mounting
Introducing
Mounting
1
3
0
0
0
3
0
1,5
0
2
10
0
7
0
17
0
8,5
0
3
2
3
0
1
2
4
1
2
4
8
2
5
7
13
9
6,5
4,5
5
3
0
2
3
5
3
2,5
1,5
4
1,6
Rerata Total
Dosis2
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
11
0
0
0
11
0
5,5
0
2
13
1
0
0
13
1
6,5
0,5
3
16
1
13
2
29
3
14,5
1,5
4
11
0
5
0
16
0
8
0
5
0
0
0
0
0
0
0
0
6,9
0,4
Rerata Total
Dosis3
Mencit
15 menit pertama
Introducing
Mounting
15 menit kedua
Introducing
Mounting
Jumlah
Introducing
Rerata
Mounting
Introducing
Mounting
1
0
0
1
0
1
0
0,5
0
2
7
0
0
0
7
0
3,5
0
3
2
0
0
0
2
0
1
0
4
25
1
19
3
44
4
22
2
5
34
4
25
3
59
7
29,5
3,5
11,3
1,1
Rerata Total
Kontrol
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
1
2
0
0
1
2
0,5
1
2
1
0
1
0
2
0
1
0
3
2
2
1
2
3
4
1,5
2
4
2
0
1
0
3
0
1,5
0
5
1
0
0
0
1
0
0,5
0
1
0,6
Rerata Total
58
Pembanding
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
10
0
4
0
14
0
7
0
2
10
0
6
0
16
0
8
0
3
15
3
10
2
25
5
12,5
2,5
4
13
6
5
0
18
6
9
3
5
10
7
4
3
14
10
7
0
8,7
1,1
Rerata Total
Hari Kelima
Dosis 1
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
2
0
1
0
3
0
1,5
0
2
12
2
10
1
22
3
11
1,5
3
0
0
0
0
0
0
0
0
4
10
2
5
2
15
4
7,5
2
5
7
1
5
0
12
1
6
0,5
5,2
0,8
Rerata Total
Dosis 2
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
3
0
6
0
9
0
4,5
0
2
23
2
25
1
48
3
24
1,5
3
13
0
11
0
24
0
12
0
4
19
1
14
1
33
2
16,5
1
5
0
0
0
0
0
0
0
0
11,4
0,5
Rerata Total
Dosis 3
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
18
2
12
1
30
3
15
1,5
2
10
1
8
0
18
1
9
0,5
3
0
0
0
0
0
0
0
0
4
1
1
0
0
1
1
0,5
0,5
5
14
2
9
0
23
2
11,5
1
7,2
0,7
Rerata Total
59
Kontrol
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
1
0
1
0
2
0
1
0
2
2
0
1
0
3
0
1,5
0
3
1
0
0
0
1
0
0,5
0
4
0
0
2
0
2
0
1
0
5
1
0
1
0
2
0
1
0
1
0
Rerata Total
Pembanding
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
19
2
15
1
34
3
17
1,5
2
25
2
18
0
43
2
21,5
1
3
10
1
18
5
28
6
14
3
4
9
3
17
1
26
4
13
2
5
8
2
6
0
14
2
7
1
14,5
1,7
Rerata Total
Hari Ketujuh
Dosis 1
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
0
0
0
0
0
0
0
0
2
27
9
13
0
40
9
20
4,5
3
9
0
4
1
13
1
6,5
0,5
4
14
2
16
4
30
6
15
3
5
6
0
10
0
16
0
8
0
9,9
1,6
Rerata Total
Dosis 2
Mencit
15 menit pertama
Introducing
Mounting
1
2
2
36
3
15 menit kedua
Introducing
Mounting
0
1
3
47
25
1
4
5
5
2
Rerata Total
Jumlah
Rerata
Introducing
Mounting
Introducing
Mounting
0
3
1
83
0
1,5
0
4
41,5
2
27
0
52
1
26
0,5
2
6
2
11
4
5,5
2
0
1
0
3
0
1,5
0
15,2
0,9
60
Dosis 3
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
10
3
11
8
21
11
10,5
5,5
2
11
3
8
0
19
3
9,5
1,5
3
0
0
0
0
0
0
0
0
4
0
0
0
0
0
0
0
0
5
7
0
4
0
11
0
5,5
0
5,1
1,4
Rerata Total
Kontrol
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
2
0
0
0
2
0
1
0
2
1
0
1
0
2
0
1
0
3
2
0
3
0
5
0
2,5
0
4
2
0
1
0
3
0
1,5
0
5
0
0
1
0
1
0
0,5
0
1,3
0
Rerata Total
Pembanding
15 menit pertama
15 menit kedua
Jumlah
Rerata
Mencit
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
Introducing
Mounting
1
23
0
12
0
35
0
17,5
0
2
4
0
5
4
9
4
4,5
2
3
11
6
8
4
19
10
9,5
5
4
11
2
4
0
15
2
7,5
1
5
7
0
0
0
7
0
Rerata Total
3,5
0
8,5
1,6
61
LAMPIRAN 4
Analisis Data
1. Introducing Hari Ketiga
Descriptives
Ln_introducing_3
EADS_1
EADS_2
EADS_3
kontrol
pembanding
Total
N
Mean
5
5
5
5
5
25
1,43
1,76
1,83
,67
2,25
1,59
Std.
Std. 95% Confidence Interval for Mean Minimum Maximum
Deviation Error Lower Bound
Upper Bound
,68
1,04
1,38
,26
,22
,94
,30
,47
,62
,11
,10
,19
,58
,47
,11
,35
1,98
1,20
2,27
3,06
3,55
,98
2,52
1,98
,69
,00
,41
,41
2,08
,00
Test of Homogeneity of Variances
Ln_introducing_3
Levene
Statistic
df1
df2
Sig.
5,220
4
20
,005
ANOVA
Ln_introducing_3
Between Groups
Within Groups
Total
Sum of Squares
df
Mean Square
F
Sig.
7,028
14,286
21,314
4
20
24
1,757
,714
2,460
,079
2,25
2,74
3,42
,92
2,60
3,42
62
2. Introducing Hari Kelima
Descriptives
Ln_introducing_5
EADS_1
EADS_2
EADS_3
kontrol
pembanding
Total
N
Mean
5
5
5
5
5
25
1,50
2,07
1,60
,68
2,69
1,71
Std.
Std. 95% Confidence Interval for Mean Minimum Maximum
Deviation Error Lower Bound
Upper Bound
1,02
1,29
1,30
,18
,39
1,10
,46
,57
,58
,08
,17
,22
,23
,47
-,01
,45
2,21
1,25
2,77
3,67
3,21
,91
3,17
2,16
,00
,00
,00
,41
2,08
,00
Test of Homogeneity of Variances
Ln_introducing_5
Levene Statistic
df1
df2
Sig.
5,960
4
20
,003
ANOVA
Ln_introducing_5
Between Groups
Within Groups
Total
Sum of Squares
df
Mean Square
F
Sig.
10,999
18,219
29,218
4
20
24
2,750
,911
3,019
,042
2,48
3,22
2,77
,92
3,11
3,22
63
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Ln_introducing_5
LSD
(I) EADS_1,
(J) EADS_1,
Mean
Std.
EADS_2, EADS_3, EADS_2, EADS_3, Difference Error
kontrol, pembanding kontrol, pembanding
(I-J)
Sig.
EADS_1
EADS_2
EADS_3
kontrol
pembanding
95% Confidence Interval
Lower Bound
Upper Bound
EADS_2
-,57
,60
,354
-1,83
,69
EADS_3
-,10
,60
,865
-1,36
1,16
kontrol
,82
,60
,191
-,44
2,08
-1,19
,57
,47
1,39*
-,62
,10
-,47
,92
-1,08
-,82
-1,39*
-,92
-2,01*
1,19
,60
,60
,60
,60
,60
,60
,60
,60
,60
,60
,60
,60
,60
,60
,063
,354
,446
,032
,320
,865
,446
,143
,087
,191
,032
,143
,003
,063
-2,45
-,69
-,79
,13
-1,88
-1,16
-1,73
-,34
-2,34
-2,08
-2,65
-2,18
-3,27
-,07
,07
1,83
1,73
2,65
,64
1,36
,79
2,18
,17
,44
-,13
,34
-,75
2,45
EADS_2
,62
,60
,320
-,64
1,88
EADS_3
1,08
,60
,087
-,17
2,34
*
,60
,003
,75
3,27
pembanding
EADS_1
EADS_3
kontrol
pembanding
EADS_1
EADS_2
kontrol
pembanding
EADS_1
EADS_2
EADS_3
pembanding
EADS_1
kontrol
2,01
*. The mean difference is significant at the 0.05 level.
64
3. Introducing Hari Ketujuh
Descriptives
Ln_introducing_7
EADS_1
EADS_2
EADS_3
kontrol
pembanding
Total
N
Mean
5
5
5
5
5
25
2,01
2,15
1,33
,79
2,12
1,68
Std.
Std. 95% Confidence Interval for Mean Minimum Maximum
Deviation Error Lower Bound
Upper Bound
1,20
1,32
1,24
,31
,56
1,07
,54
,59
,55
,14
,25
,21
,52
,51
-,20
,40
1,43
1,24
3,49
3,79
2,87
1,18
2,82
2,12
,00
,92
,00
,41
1,50
,00
Test of Homogeneity of Variances
Ln_introducing_7
Levene Statistic
3,404
df1
4
df2
20
Sig.
,028
ANOVA
Ln_introducing_7
Between Groups
Within Groups
Total
Sum of Squares
df
Mean Square
F
Sig.
7,162
20,470
27,632
4
20
24
1,790
1,024
1,749
,179
3,04
3,75
2,44
1,25
2,92
3,75
65
4. Rerata Total Introducing Hari Ketiga, Kelima, dan Ketujuh
Descriptives
Ln_total_introducing
EADS_1
EADS_2
EADS_3
kontrol
pembanding
Total
N
Mean
5
5
5
5
5
25
1,77
2,10
1,92
,73
2,41
1,79
Std.
Std. 95% Confidence Interval for Mean Minimum Maximum
Deviation Error Lower Bound
Upper Bound
,80
1,14
,97
,16
,30
,91
,36
,51
,43
,07
,13
,18
,77
,69
,72
,53
2,04
1,41
2,77
3,51
3,12
,94
2,79
2,16
,69
,41
,26
,53
1,92
,26
Test of Homogeneity of Variances
Ln_total_introducing
Levene Statistic
df1
df2
Sig.
3,003
4
20
,043
ANOVA
Ln_total_introducing
Between Groups
Within Groups
Total
Sum of Squares
df
Mean Square
F
Sig.
8,118
11,948
20,065
4
20
24
2,029
,597
3,397
,028
2,65
3,22
2,80
,92
2,69
3,22
66
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Ln_total_introducing
LSD
(I) EADS_1,
(J) EADS_1,
Mean
Std.
EADS_2, EADS_3, EADS_2, EADS_3, Difference Error
kontrol, pembanding kontrol, pembanding
(I-J)
Sig.
EADS_1
EADS_2
EADS_3
kontrol
pembanding
95% Confidence Interval
Lower Bound
Upper Bound
EADS_2
-,33
,49
,503
-1,35
,69
EADS_3
-,15
,49
,761
-1,17
,87
kontrol
1,04*
,49
,047
,02
2,06
pembanding
EADS_1
EADS_3
kontrol
pembanding
EADS_1
EADS_2
kontrol
pembanding
EADS_1
EADS_2
EADS_3
pembanding
EADS_1
-,64
,33
,18
1,37*
-,31
,15
-,18
1,19*
-,49
-1,04*
-1,37*
-1,19*
-1,68*
,64
,49
,49
,49
,49
,49
,49
,49
,49
,49
,49
,49
,49
,49
,49
,202
,503
,712
,011
,531
,761
,712
,025
,324
,047
,011
,025
,003
,202
-1,66
-,69
-,84
,35
-1,33
-,87
-1,20
,17
-1,51
-2,06
-2,39
-2,21
-2,70
-,37
,37
1,35
1,20
2,39
,71
1,17
,84
2,21
,53
-,02
-,35
-,17
-,66
1,66
EADS_2
,31
,49
,531
-,71
1,33
EADS_3
,49
,49
,324
-,53
1,51
1,68*
,49
,003
,66
2,70
kontrol
*. The mean difference is significant at the 0.05 level.
67
5. Mounting Hari Ketiga
Descriptives
Ln_mounting_3
EADS_1
EADS_2
EADS_3
Kontrol
pembanding
Total
N
Mean
5
5
5
5
5
25
,74
,26
,52
,36
,53
,48
Std.
Std. 95% Confidence Interval for Mean Minimum Maximum
Deviation Error Lower Bound
Upper Bound
,74
,40
,73
,51
,72
,60
,33
,18
,33
,23
,32
,12
-,17
-,24
-,38
-,28
-,37
,23
1,66
,77
1,42
,99
1,43
,73
,00
,00
,00
,00
,00
,00
Test of Homogeneity of Variances
Ln_mounting_3
Levene Statistic
df1
df2
Sig.
2,086
4
20
,121
ANOVA
Ln_mounting_3
Between Groups
Within Groups
Total
Sum of Squares
df
Mean Square
F
Sig.
,674
8,098
8,772
4
20
24
,169
,405
,416
,795
1,70
,92
1,50
1,10
1,39
1,70
68
6. Mounting Hari Kelima
Descriptives
Ln_mounting_5
N
EADS_1
EADS_2
EADS_3
kontrol
pembanding
Total
5
5
5
5
5
25
Mean
Std.
Std. 95% Confidence Interval for Mean Minimum Maximum
Deviation Error Lower Bound
Upper Bound
,48
,32
,48
,48
,96
,55
,51
,45
,35
,35
,29
,42
,23
,20
,15
,15
,13
,08
-,15
-,23
,06
,06
,59
,37
1,12
,88
,91
,91
1,32
,72
Test of Homogeneity of Variances
Ln_mounting_5
Levene Statistic
df1
df2
Sig.
1,252
4
20
,321
ANOVA
Ln_mounting_5
Between Groups
Within Groups
Total
Sum of Squares
df
Mean Square
F
Sig.
1,155
3,141
4,296
4
20
24
,289
,157
1,839
,161
,00
,00
,00
,00
,69
,00
1,10
,92
,92
,92
1,39
1,39
69
7. Mounting Hari Ketujuh
Descriptives
Ln_mounting_7
EADS_1
EADS_2
EADS_3
kontrol
pembanding
Total
N
Mean
5
5
5
5
5
25
,70
,52
,56
,00
,72
,50
Std.
Std. 95% Confidence Interval for Mean Minimum Maximum
Deviation Error Lower Bound
Upper Bound
,80
,55
,83
,00
,76
,66
,36
,25
,37
,00
,34
,13
-,29
-,17
-,48
,00
-,23
,22
1,69
1,21
1,59
,00
1,66
,77
,00
,00
,00
,00
,00
,00
Test of Homogeneity of Variances
Ln_mounting_7
Levene Statistic
df1
df2
Sig.
4,963
4
20
,006
ANOVA
Ln_mounting_7
Between Groups
Within Groups
Total
Sum of Squares
df
Mean Square
F
Sig.
1,702
8,889
10,591
4
20
24
,426
,444
,957
,452
1,70
1,10
1,87
,00
1,79
1,87
70
8. Rerata Total Mounting Hari Ketiga, Kelima, dan Ketujuh
Descriptives
Ln_total_mounting
EADS_1
EADS_2
EADS_3
kontrol
pembanding
Total
N
Mean
5
5
5
5
5
25
,73
,41
,65
,16
,79
,55
Std.
Std. 95% Confidence Interval for Mean Minimum Maximum
Deviation Error Lower Bound
Upper Bound
,55
,39
,45
,24
,51
,47
,25
,17
,20
,11
,23
,09
,04
-,07
,09
-,14
,16
,35
1,42
,90
1,20
,45
1,43
,74
,00
,00
,00
,00
,26
,00
Test of Homogeneity of Variances
Ln_total_mounting
Levene Statistic
df1
df2
Sig.
,972
4
20
,444
ANOVA
Ln_total_mounting
Between Groups
Within Groups
Total
Sum of Squares
df
Mean Square
F
Sig.
1,365
3,908
5,274
4
20
24
,341
,195
1,746
,179
1,44
,83
1,19
,53
1,50
1,50
71
LAMPIRAN 5
Foto Penelitian
Alat dan Bahan Penelitian
Daun stroberi kering ditumbuk
Daun stroberi kering
menggunakan mortir dan stemper
Kandang penyimpanan mencit
Kandang pengamatan mencit
Pengamatan Hewan Coba
Introducing
Mounting
72
LAMPIRAN 6
KOMISI ETIK
RIWAYAT HIDUP
Data Pribadi
Nama
: Sharin Nadya Megaputri
NRP
: 0910130
Tempat dan Tanggal Lahir
: Bandung, 25 Maret 1990
Alamat
: Jalan Setiabudi No. 165B, Bandung 40153
Riwayat Pendidikan
• TK St. Yusuf, Bandung
1995-1997
• SD St. Yusuf, Bandung
1997-2003
• SMP St. Angela, Bandung
2003-2006
• SMA St. Angela, Bandung
2006-2009
• Mahasiswa Fakultas Kedokteran
Universitas Kristen Maranatha
2009-sekarang
73