In Vitro

In Vitro Data for Substances Tested in the ICE Test Method: Sorted by Reference
Substance/Product Name
Acetone
Acetone
Acetone
Acetone
Ammonium nitrate
Ammonium nitrate
Ammonium nitrate
Ammonium nitrate
L-Aspartic acid
L-Aspartic acid
L-Aspartic acid
L-Aspartic acid
Benzalkonium chloride (1%)
Benzalkonium chloride (1%)
Benzalkonium chloride (1%)
Benzalkonium chloride (1%)
Benzalkonium chloride (10%)
Benzalkonium chloride (10%)
Benzalkonium chloride (10%)
Benzalkonium chloride (10%)
Benzalkonium chloride (5%)
Benzalkonium chloride (5%)
Benzalkonium chloride (5%)
Benzalkonium chloride (5%)
n-Butyl acetate
n-Butyl acetate
n-Butyl acetate
n-Butyl acetate
Gammabutyrolactone
Gammabutyrolactone
Gammabutyrolactone
Gammabutyrolactone
Captan 90 concentrate
Captan 90 concentrate
Captan 90 concentrate
Captan 90 concentrate
4-Carboxybenzaldehyde
4-Carboxybenzaldehyde
4-Carboxybenzaldehyde
4-Carboxybenzaldehyde
Cetylpyridinium bromide (0.1%)
Cetylpyridinium bromide (0.1%)
Cetylpyridinium bromide (0.1%)
Cetylpyridinium bromide (0.1%)
CASRN
67-64-1
67-64-1
67-64-1
67-64-1
6484-52-2
6484-52-2
6484-52-2
6484-52-2
70-47-3
70-47-3
70-47-3
70-47-3
8001-54-5
8001-54-5
8001-54-5
8001-54-5
8001-54-5
8001-54-5
8001-54-5
8001-54-5
8001-54-5
8001-54-5
8001-54-5
8001-54-5
123-86-4
123-86-4
123-86-4
123-86-4
96-48-0
96-48-0
96-48-0
96-48-0
133-06-2
133-06-2
133-06-2
133-06-2
619-66-9
619-66-9
619-66-9
619-66-9
140-72-7
140-72-7
140-72-7
140-72-7
Water
Form
Tested
Solubility
liquid
liquid
liquid
liquid
solid
solid
solid
solid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
I*
I*
I*
I*
S
S
S
S
S
S
S
S
I*
I*
I*
I*
Sf
Sf
Sf
Sf
1
Concentration
Tested
Purity
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
1%
1%
1%
1%
10%
10%
10%
10%
5%
5%
5%
5%
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
neat
neat
neat
neat
0.1%
0.1%
0.1%
0.1%
99%
99%
99%
99%
>99.9%
>99.9%
>99.9%
>99.9%
100%
100%
100%
100%
98%
98%
98%
98%
98%
98%
98%
98%
98%
98%
98%
98%
99%
99%
99%
99%
>99%
>99%
>99%
>99%
90%
90%
90%
90%
95%
95%
95%
95%
98%
98%
98%
98%
Source
Fisher
Fisher
Fisher
Fisher
Aldrich
Aldrich
Aldrich
Aldrich
Degussa
Degussa
Degussa
Degussa
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Fisher
Fisher
Fisher
Fisher
Aldrich
Aldrich
Aldrich
Aldrich
EPA
EPA
EPA
EPA
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Lab
No.
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
Fluorescein Corneal
Retention Opacity
1.4
1
1.83
3
1.2
2
1.33
2
1
2
1.83
2
1.8
1.3
2.67
2
3
3
3
3
3
1
3
3
1.8
0.5
1
1
2.6
1.3
1.67
1
0
0.2
0
1
1
1.3
0.67
2
1
0.7
0.67
1
0.4
1.7
1.17
1
0.9
1.3
1.5
0.5
0.7
2
1.67
1
0.6
2.3
1.5
3
3
2.3
2.33
2
2.6
2
2
3
1.8
2.7
2
2
1.4
2
1.5
2
0.4
1
1.33
1
0.5
3
0.5
1
0
0
1
1
Corneal
Swelling
9.6
49
7.64
13.8
6.7
42
12.33
6
3.2
56
14.67
10
18
47
12.66
8.8
37.7
95
40.72
41.1
36.3
42
33.77
68.9
13.9
42
14.67
32.2
15.8
47
13.1
13
1.7
27
19.17
20
5.4
89
-1.4
12.7
2.2
21
10.29
14.6
In Vitro
Classif.
(GHS)1,2
2B
2A
2B
2A
2B
2A
2B
2B
2B
2A
2A
2B
2A/2B
2A
2A
2A
1
1
1
1
1
2A
1
1
2A
1
2A/2B
2A
2A
2A
2A/2B
2A/2B
NI
2B
2B
2B
NI
1
NI
2A/2B
NI
2B
2B
2B
Overall
In Vitro
Classif.
(GHS)
2A
2B
2A
2A
1
1
2A
2A
2B
NI
2B
In Vitro
Classif.
(EPA)3,4
III
II
III
II
III
II
III
III
III
II
II
III
II/III
II
II
II
I
I
I
I
I
II
I
I
II
I
II/III
II
II
II
II/III
II/III
IV
III
III
III
IV
I
IV
II/III
IV
III
III
III
Overall
In Vitro
Classif.
(EPA)
II
III
II
II
I
I
II
II
III
IV
III
In Vitro
Classif.
(EU)5,6
NI
R36
R36
R36
NI
R36
NI
NI
NI
R36
R36
R36
R36
R36
R36
R36
R41
R41
R41
R41
R41
R36
R41
R41
R36
R41
R36
R36
R36
R36
R36
R36
NI
NI/R36
NI
R36
NI
R41
NI
R36
NI
NI
NI
NI/R36
Overall
In Vitro
Classif.
(EU)
R36
NI
R36
R36
R41
R41
R36
R36
NI
NI
NI
Reference
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
In Vitro Data for Substances Tested in the ICE Test Method: Sorted by Reference
Substance/Product Name
Cetylpyridinium bromide (10%)
Cetylpyridinium bromide (10%)
Cetylpyridinium bromide (10%)
Cetylpyridinium bromide (10%)
Cetylpyridinium bromide (6%)
Cetylpyridinium bromide (6%)
Cetylpyridinium bromide (6%)
Cetylpyridinium bromide (6%)
Chlorhexidine
Chlorhexidine
Chlorhexidine
Chlorhexidine
Cyclohexanol
Cyclohexanol
Cyclohexanol
Cyclohexanol
Dibenzoyl-L-tartaric acid
Dibenzoyl-L-tartaric acid
Dibenzoyl-L-tartaric acid
Dibenzoyl-L-tartaric acid
Dibenzyl phosphate
Dibenzyl phosphate
Dibenzyl phosphate
Dibenzyl phosphate
2,6-Dichlorobenzoyl chloride
2,6-Dichlorobenzoyl chloride
2,6-Dichlorobenzoyl chloride
2,6-Dichlorobenzoyl chloride
2,2-Dimethylbutanoic acid
2,2-Dimethylbutanoic acid
2,2-Dimethylbutanoic acid
2,2-Dimethylbutanoic acid
2,5-Dimethylohexanediol
2,5-Dimethylohexanediol
2,5-Dimethylohexanediol
2,5-Dimethylohexanediol
Ethanol
Ethanol
Ethanol
Ethanol
Ethyl acetate
Ethyl acetate
Ethyl acetate
Ethyl acetate
CASRN
140-72-7
140-72-7
140-72-7
140-72-7
140-72-7
140-72-7
140-72-7
140-72-7
55-56-1
55-56-1
55-56-1
55-56-1
108-93-0
108-93-0
108-93-0
108-93-0
2743-38-6
2743-38-6
2743-38-6
2743-38-6
1623-08-1
1623-08-1
1623-08-1
1623-08-1
4659-45-4
4659-45-4
4659-45-4
4659-45-4
595-37-9
595-37-9
595-37-9
595-37-9
110-03-2
110-03-2
110-03-2
110-03-2
64-17-5
64-17-5
64-17-5
64-17-5
141-78-6
141-78-6
141-78-6
141-78-6
Water
Form
Tested
Solubility
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
Sf
Sf
Sf
Sf
Sf
Sf
Sf
Sf
I*
I*
I*
I*
S
S
S
S
I
I
I
I
I*
I*
I*
I*
I*
I*
I*
I*
I
I
I
I
I
I
I
I
S
S
S
S
S
S
S
S
1
Concentration
Tested
Purity
10%
10%
10%
10%
6%
6%
6%
6%
neat
neat
neat
neat
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
neat
neat
neat
neat
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
98%
98%
98%
98%
98%
98%
98%
98%
n.p.
n.p.
n.p.
n.p.
97%
97%
97%
97%
98%
98%
98%
98%
99%
99%
99%
99%
99%
99%
99%
99%
96%
96%
96%
96%
99.5%
99.5%
99.5%
99.5%
n.p.
n.p.
n.p.
n.p.
99%
99%
99%
99%
Source
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Degussa
Degussa
Degussa
Degussa
Fisher
Fisher
Fisher
Fisher
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
BASF
BASF
BASF
BASF
Local vendor
Local vendor
Local vendor
Local vendor
Fisher
Fisher
Fisher
Fisher
Lab
No.
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
Fluorescein Corneal
Retention Opacity
2.6
1.7
2
3
2
2
3
2.7
3
3
3
3
2.2
3
3
3
2.8
1
2
1
2.6
1
2
2
2.3
2
1.83
1.8
3
3
3
3
2
3
1.33
2
2.8
2
2.5
2
2
1.7
2
3
1
2
1.67
3
1.2
0.5
1.83
1.7
4
4
3
4
2.2
2
2.5
2.5
3
2.7
1.5
2
2
0
1.5
2
0.8
1.3
1.67
0.8
2.4
2.7
2.5
3
1
3
1.67
1
2.8
3
2.33
2.3
2
2.3
2
2
Corneal
Swelling
25.8
41
27.2
17.8
27.2
49
24.55
13.5
32
150
53.13
24.7
103
35.74
45.3
12.8
75
6.36
6.7
12.2
22
17.07
40.9
12.7
26
17.15
16.8
43.8
74
35.9
62.7
11.9
64
11.57
6.7
30.7
74
35.88
34.6
22
76
25.08
23
In Vitro
Classif.
(GHS)1,2
2A
2A
2A
1
2A
2A
2A
2A
1
1
1
1
2A
1
1
1
1
1
2B
2B
2A
2B
2A/2B
1
2A/2B
2A
2A
2A/2B
1
1
1
1
2B
1
2B
2B
1
1
2A
2A
2A
2A
2A
2A
Overall
In Vitro
Classif.
(GHS)
2A
2A
1
1
1
2A/2B
2A
1
2B
1
2A
In Vitro
Classif.
(EPA)3,4
II
II
II
I
II
II
II
II
I
I
I
I
II
I
I
I
I
I
III
III
II
III
II/III
I
II/III
II
II
II/III
I
I
I
I
III
I
III
III
I
I
II
II
II
II
II
II
Overall
In Vitro
Classif.
(EPA)
II
II
I
I
I
II/III
II
I
III
I
II
In Vitro
Classif.
(EU)5,6
R36
R36
R36
R41
R36
R36
R36
R36
R41
R41
R41
R41
R36
R41
R41
R41
R41
R41
R36
R36
R36
R36
R36
R36
R36
R36
R36
R36
R41
R41
R41
R41
R36
R41
R36
R36
R41
R41
R36
R36
R36
R36
R36
R36
Overall
In Vitro
Classif.
(EU)
R36
R36
R41
R41
R41
R36
R36
R41
R36
R41
R36
Reference
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
In Vitro Data for Substances Tested in the ICE Test Method: Sorted by Reference
Substance/Product Name
2-Ethyl-1-hexanol
2-Ethyl-1-hexanol
2-Ethyl-1-hexanol
2-Ethyl-1-hexanol
Ethyl-2-methylacetoacetate
Ethyl-2-methylacetoacetate
Ethyl-2-methylacetoacetate
Ethyl-2-methylacetoacetate
Ethyl trimethyl acetate
Ethyl trimethyl acetate
Ethyl trimethyl acetate
Ethyl trimethyl acetate
Fomesafen
Fomesafen
Fomesafen
Fomesafen
Glycerol
Glycerol
Glycerol
Glycerol
n-Hexanol
n-Hexanol
n-Hexanol
n-Hexanol
Imidazole
Imidazole
Imidazole
Imidazole
Isobutanol
Isobutanol
Isobutanol
Isobutanol
Isopropanol
Isopropanol
Isopropanol
Isopropanol
Maneb
Maneb
Maneb
Maneb
Methyl acetate
Methyl acetate
Methyl acetate
Methyl acetate
CASRN
104-76-7
104-76-7
104-76-7
104-76-7
609-14-3
609-14-3
609-14-3
609-14-3
3938-95-2
3938-95-2
3938-95-2
3938-95-2
72128-02-0
72128-02-0
72128-02-0
72128-02-0
56-81-5
56-81-5
56-81-5
56-81-5
111-27-3
111-27-3
111-27-3
111-27-3
288-32-4
288-32-4
288-32-4
288-32-4
78-83-1
78-83-1
78-83-1
78-83-1
67-63-0
67-63-0
67-63-0
67-63-0
12427-38-2
12427-38-2
12427-38-2
12427-38-2
79-20-9
79-20-9
79-20-9
79-20-9
Water
Form
Tested
Solubility
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
S
S
S
S
S*
S*
S*
S*
I*
I*
I*
I*
S
S
S
S
S
S
S
S
I*
I*
I*
I*
S
S
S
S
I*
I*
I*
I*
S
S
S
S
S
S
S
S
S
S
S
S
1
Concentration
Tested
Purity
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
undiluted
undiluted
undiluted
undiluted
99%
99%
99%
99%
97%
97%
97%
97%
99%
99%
99%
99%
97.5%
97.5%
97.5%
97.5%
>99.5%
>99.5%
>99.5%
>99.5%
98%
98%
98%
98%
99%
99%
99%
99%
99.9%
99.9%
99.9%
99.9%
99.9%
99.9%
99.9%
99.9%
90%
90%
90%
90%
98%
98%
98%
98%
Source
Fisher
Fisher
Fisher
Fisher
Fluka
Fluka
Fluka
Fluka
Aldrich
Aldrich
Aldrich
Aldrich
EPA
EPA
EPA
EPA
Mallinkrodt
Mallinkrodt
Mallinkrodt
Mallinkrodt
E-Kodak
E-Kodak
E-Kodak
E-Kodak
Aldrich
Aldrich
Aldrich
Aldrich
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
EPA
EPA
EPA
EPA
Fisher
Fisher
Fisher
Fisher
Lab
No.
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
Fluorescein Corneal
Retention Opacity
2
1
3
1
0.4
1
0.67
1
1.2
2
0
1
0.9
0
1
1
1.2
0
1.17
2
2.8
0.2
3
3
3
3
3
3
2.8
3
3
3
2
0.7
3
2.3
0
1
0
1
1.4
1
2
3
2.2
2.3
1.5
2
0.3
0
1
0.5
0.4
2
0
0.5
1.2
0.2
0.5
1
1
0
1
0.5
1.6
1.7
2.83
3
4
3
2.5
3
2.5
2.7
2.5
2
1.6
2.7
2.5
0.5
0.5
2
0.5
1
2.4
2.7
2
3
Corneal
Swelling
43
62
13.31
52.4
-2.8
7
11.52
4.5
7.2
31
1.44
6.7
5.3
11
2.82
4.3
5
11
8.3
29.4
17.4
82
28.89
58.9
40.3
224
36.96
97.8
46.4
93
37.06
69.2
23.3
72
37.84
8.9
2.8
33
8.03
6.7
20.3
93
22.5
17.5
In Vitro
Classif.
(GHS)1,2
2A
2A
2A
2A
NI
2B
2B
NI
2B
2A
NI
2B
2B
NI
NI
2B
2B
NI
2B
2A
2A
2A
1
1
1
1
1
1
1
1
1
1
2A
1
1
2B
NI
2A
NI
2B
2A
1
2A
1
Overall
In Vitro
Classif.
(GHS)
2A
2B
2B
2B
2B
1
1
1
1
NI
1
In Vitro
Classif.
(EPA)3,4
II
II
II
II
IV
III
III
IV
III
II
IV
III
III
IV
IV
III
III
IV
III
II
II
II
I
I
I
I
I
I
I
I
I
I
II
I
I
III
IV
II
IV
III
II
I
II
I
Overall
In Vitro
Classif.
(EPA)
II
III
III
III
III
I
I
I
I
IV
I
In Vitro
Classif.
(EU)5,6
R36
R36
R36
R36
NI
NI
NI
NI
NI
R36
NI
NI
NI
NI
NI
NI
NI
NI
NI
R36
R36
R36
R41
R41
R41
R41
R41
R41
R41
R41
R41
R41
R36
R41
R41
NI
NI
R36
NI
NI
R36
R41
R36
R41
Overall
In Vitro
Classif.
(EU)
R36
NI
NI
NI
NI
R41
R41
R41
R41
NI
R41
Reference
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
In Vitro Data for Substances Tested in the ICE Test Method: Sorted by Reference
Substance/Product Name
Methyl cyanoacetate
Methyl cyanoacetate
Methyl cyanoacetate
Methyl cyanoacetate
Methylcyclopentane
Methylcyclopentane
Methylcyclopentane
Methylcyclopentane
Methyl ethyl ketone
Methyl ethyl ketone
Methyl ethyl ketone
Methyl ethyl ketone
Methyl isobutyl ketone
Methyl isobutyl ketone
Methyl isobutyl ketone
Methyl isobutyl ketone
1-Napthaleneacetic acid
1-Napthaleneacetic acid
1-Napthaleneacetic acid
1-Napthaleneacetic acid
1-Napthaleneacetic acid, sodium
salt
1-Napthaleneacetic acid, sodium
salt
1-Napthaleneacetic acid, sodium
salt
1-Napthaleneacetic acid, sodium
salt
n-Octanol
n-Octanol
n-Octanol
n-Octanol
Parafluoraniline
Parafluoraniline
Parafluoraniline
Parafluoraniline
Polyethylene glycol 400
Polyethylene glycol 400
Polyethylene glycol 400
Polyethylene glycol 400
Potassium cyanate
Potassium cyanate
Potassium cyanate
Potassium cyanate
Water
Form
Tested
Concentration
Tested
Purity
Solubility
105-34-0
105-34-0
105-34-0
105-34-0
96-37-7
96-37-7
96-37-7
96-37-7
78-93-3
78-93-3
78-93-3
78-93-3
108-10-1
108-10-1
108-10-1
108-10-1
86-87-3
86-87-3
86-87-3
86-87-3
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
S*
S*
S*
S*
I*
I*
I*
I*
S
S
S
S
I*
I*
I*
I*
I*
I*
I*
I*
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
99%
99%
99%
99%
>99%
>99%
>99%
>99%
99%
99%
99%
99%
98%
98%
98%
98%
96%
96%
96%
96%
Aldrich
Aldrich
Aldrich
Aldrich
Fluka
Fluka
Fluka
Fluka
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
EPA
EPA
EPA
EPA
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
0.4
0.5
0.17
1
0.4
1
0
1
2
2.7
3
3
2.6
3
2
2
1
1
2
1
61-31-4
61-31-4
solid
S*
neat
95%
EPA
1
solid
S*
neat
95%
EPA
2
61-31-4
solid
S*
neat
95%
EPA
61-31-4
solid
S*
neat
95%
111-87-5
111-87-5
111-87-5
111-87-5
371-40-4
371-40-4
371-40-4
371-40-4
25322-68-3
25322-68-3
25322-68-3
25322-68-3
590-28-3
590-28-3
590-28-3
590-28-3
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
I*
I*
I*
I*
I
I
I
I
Sf
Sf
Sf
Sf
S
S
S
S
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
>99%
>99%
>99%
>99%
99%
99%
99%
99%
n.p.
n.p.
n.p.
n.p.
97%
97%
97%
97%
CASRN
1
Source
Lab
No.
Fluorescein Corneal
Retention Opacity
Corneal
Swelling
In Vitro
Classif.
(GHS)1,2
Overall
In Vitro
Classif.
(GHS)
In Vitro
Classif.
(EPA)3,4
In Vitro
Classif.
(EU)5,6
0.3
0.7
0.5
1
0.5
0
0.5
0.5
2.2
3
2.33
2
2
3
2.17
2
0.9
1
1
1
4.5
44
4.93
10.7
2.3
22
5.83
0
23.1
99
34.88
12.6
26.5
64
21.69
12.3
5.6
24
8.86
46.7
NI
2A
NI
2B
NI
2B
NI
NI
2A
1
1
2A
2A
1
2A
2A
2B
2B
2B
2A
3
3
46.6
1
I
R41
3
2.7
122
1
I
R41
3
3
2.5
44.19
1
EPA
4
3
3
64.1
1
I
R41
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Degussa
Degussa
Degussa
Degussa
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
2
1.3
1.17
2
3
3
3
3
1.4
0.2
2
1
1
0.2
1.67
1.3
2.4
2
1.5
1
2.2
2
2
2
1
0
1
0.5
0.7
0
1
1.7
36.5
108
10.18
25.7
35.3
79
33.44
38.5
9.8
26
5.88
14.8
8
25
10.45
25.3
2A
2A
2B
2A
1
1
1
1
2B
NI
2B
2B
2B
2B
2B
2A
II
II
III
II
I
I
I
I
III
IV
III
III
III
III
III
II
R36
R36
NI
R36
R41
R41
R41
R41
NI
NI
R36
R36
NI
NI
R36
R36
NI
NI
1
2A
2B
1
2A
1
2B
2B
IV
II
IV
III
IV
III
IV
IV
II
I
I
II
II
I
II
II
III
III
III
II
Overall
In Vitro
Classif.
(EPA)
I
IV
IV
I
II
III
I
II
I
III
III
NI
R36
NI
NI
NI
NI
NI
NI
R36
R41
R41
R36
R36
R41
R36
R36
NI
R36
R36
R36
R41
Overall
In Vitro
Classif.
(EU)
NI
NI
R41
R36
R36
Reference
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
R41
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
R36
R41
R36
R36
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
In Vitro Data for Substances Tested in the ICE Test Method: Sorted by Reference
Substance/Product Name
Promethazine HCl
Promethazine HCl
Promethazine HCl
Promethazine HCl
Pyridine
Pyridine
Pyridine
Pyridine
Quinacrine
Quinacrine
Quinacrine
Quinacrine
Sodium hydroxide (1%)
Sodium hydroxide (1%)
Sodium hydroxide (1%)
Sodium hydroxide (1%)
Sodium hydroxide (10%)
Sodium hydroxide (10%)
Sodium hydroxide (10%)
Sodium hydroxide (10%)
Sodium lauryl sulfate (15%)
Sodium lauryl sulfate (15%)
Sodium lauryl sulfate (15%)
Sodium lauryl sulfate (15%)
Sodium lauryl sulfate (3%)
Sodium lauryl sulfate (3%)
Sodium lauryl sulfate (3%)
Sodium lauryl sulfate (3%)
Sodium oxalate
Sodium oxalate
Sodium oxalate
Sodium oxalate
Sodium perborate, 4H2O
Sodium perborate, 4H2O
Sodium perborate, 4H2O
Sodium perborate, 4H2O
Tetraaminopyrimidine sulfate
Tetraaminopyrimidine sulfate
Tetraaminopyrimidine sulfate
Tetraaminopyrimidine sulfate
Toluene
Toluene
Toluene
Toluene
CASRN
58-33-3
58-33-3
58-33-3
58-33-3
110-86-1
110-86-1
110-86-1
110-86-1
69-05-6
69-05-6
69-05-6
69-05-6
1310-73-2
1310-73-2
1310-73-2
1310-73-2
1310-73-2
1310-73-2
1310-73-2
1310-73-2
151-21-3
151-21-3
151-21-3
151-21-3
151-21-3
151-21-3
151-21-3
151-21-3
62-76-0
62-76-0
62-76-0
62-76-0
10486-00-7
10486-00-7
10486-00-7
10486-00-7
5392-28-9
5392-28-9
5392-28-9
5392-28-9
108-88-3
108-88-3
108-88-3
108-88-3
Water
Form
Tested
Solubility
solid
solid
solid
solid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
solid
solid
solid
solid
solid
solid
solid
solid
solid
solid
liquid
liquid
liquid
liquid
S*
S*
S*
S*
S
S
S
S
S*
S*
S*
S*
S
S
S
S
S
S
S
S
Sf
Sf
Sf
Sf
Sf
Sf
Sf
Sf
S
S
S
S
S
S
S
S
I*
I*
I*
I*
I*
I*
I*
I*
1
Concentration
Tested
neat
neat
neat
neat
undiluted
undiluted
undiluted
undiluted
neat
neat
neat
neat
1%
1%
1%
1%
10%
10%
10%
10%
15%
15%
15%
15%
3%
3%
3%
3%
neat
neat
neat
neat
neat
neat
neat
neat
neat
neat
neat
neat
undiluted
undiluted
undiluted
undiluted
Purity
98%
98%
98%
98%
>99.9%
>99.9%
>99.9%
>99.9%
n.p.
n.p.
n.p.
n.p.
RG
RG
RG
RG
RG2
RG
RG
RG
98%
98%
98%
98%
98%
98%
98%
98%
>99%
>99%
>99%
>99%
98.6%
98.6%
98.6%
98.6%
97%
97%
97%
97%
99%
99%
99%
99%
Source
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Aldrich
Sigma
Sigma
Sigma
Sigma
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Aldrich
Aldrich
Aldrich
Aldrich
Dupont
Dupont
Dupont
Dupont
Aldrich
Aldrich
Aldrich
Aldrich
Fisher
Fisher
Fisher
Fisher
Lab
No.
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
2
3
4
1
Fluorescein Corneal
Retention Opacity
2.6
3
3
2
3
3
3
3
1.2
0.2
2
1
1
0.7
2.33
2
3
3
3
3
0.6
1
1.67
1
1
0
1
1
0.7
0.2
0.5
1
0.6
0.2
1.33
1
1.3
1
1.5
1
1.4
2
1.33
1
1.6
3
2
3
2
3
2.5
3
0.6
0.2
2
0.5
0.6
2.3
2.5
2
4
3.3
3.17
4
0.4
0.2
1
1
0.2
0
0
1
0.7
0
0.5
0
0.5
0.7
1
0.5
1
2
1.5
1
1
1.3
2
2
Corneal
Swelling
33
143
23.02
28.6
32.7
95
37.47
78.6
4.1
12
11.49
6.8
14.1
55
30.31
33.3
32
194
68.86
151.7
7
33
9.56
12.2
3.9
39
2.75
15.9
6.3
24
2.62
2.4
3.1
23
7.54
14.6
7.5
31
7.3
8.9
5.2
29
13.87
58.2
In Vitro
Classif.
(GHS)1,2
1
1
2A
1/2A
1
1
1
1
2B
NI
2A
2B
2B
2A
2A
2A
1
1
1
1
2B
2A
2B
2B
NI
2B
NI
2B
2B
2B
NI
NI
NI
2B
2B
2B
2B
2A
2B
2B
2B
2A
2A/2B
2A
Overall
In Vitro
Classif.
(GHS)
1
1
2B
2A
1
2B
2B
2B
2B
2B
2A
In Vitro
Classif.
(EPA)3,4
I
I
II
I/II
I
I
I
I
III
IV
II
III
III
II
II
II
I
I
I
I
III
II
III
III
IV
III
IV
III
III
III
IV
IV
IV
III
III
III
III
II
III
III
III
II
II/III
II
Overall
In Vitro
Classif.
(EPA)
I
I
III
II
I
III
III
III
III
III
II
In Vitro
Classif.
(EU)5,6
R41
R41
R36
R36/R41
R41
R41
R41
R41
NI
NI
R36
NI
NI/R36
R36
R36
R36
R41
R41
R41
R41
NI
R36
R36
NI
NI
R36
NI
NI/R36
NI
NI
NI
NI
NI
NI
NI
NI
NI
R36
NI
NI
NI
R36
R36
R36
Overall
In Vitro
Classif.
(EU)
R41
R41
NI
R36
R41
R36
NI
NI
NI
NI
R36
Reference
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
In Vitro Data for Substances Tested in the ICE Test Method: Sorted by Reference
Substance/Product Name
Trichloroacetic acid (3%)
Trichloroacetic acid (3%)
Trichloroacetic acid (3%)
Trichloroacetic acid (3%)
Trichloroacetic acid (30%)
Trichloroacetic acid (30%)
Trichloroacetic acid (30%)
Trichloroacetic acid (30%)
Triton X-100 (10%)
Triton X-100 (10%)
Triton X-100 (10%)
Triton X-100 (10%)
Triton X-100 (5%)
Triton X-100 (5%)
Triton X-100 (5%)
Triton X-100 (5%)
Tween 20
Tween 20
Tween 20
Tween 20
TNO-01 (Formulation-1)7
TNO-02 (Formulation-2)
TNO-03 (Pesticide-1)
TNO-04 (Detergent-1)
TNO-05 (Silicone powder-1)
TNO-06 (Lubricant)
TNO-07 (Ink-1)
TNO-08 (Ink-2)
TNO-09 (Paint)
TNO-10 (Silicone powder-2)
TNO-11 (Sodium p-styrene
sulfonate)
TNO-12 (Formulation-3)
TNO-13 (Pesticide-2)
TNO-14 (Polydisaccharide)
TNO-15 (Polydisaccharide)
TNO-16 (Liquid nylon product)
TNO-17 (Solvent-1)
TNO-18 (Solvent-2)
TNO-19 (Solvent-3)
TNO-20 (Solvent-4)
TNO-21 (Solvent-5)
TNO-22 (Solvent-6)
TNO-23 (Solvent-7)
TNO-24 (Solvent-8)
TNO-25 (Solvent-9)
Water
Form
Tested
Solubility
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
solid
gel
liquid
liquid
liquid
solid
2695-37-6
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
CASRN
76-03-9
76-03-9
76-03-9
76-03-9
76-03-9
76-03-9
76-03-9
76-03-9
9002-93-1
9002-93-1
9002-93-1
9002-93-1
9002-93-1
9002-93-1
9002-93-1
9002-93-1
9005-64-5
9005-64-5
9005-64-5
9005-64-5
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
Concentration
Tested
Purity
S
S
S
S
S
S
S
S
Sf
Sf
Sf
Sf
Sf
Sf
Sf
Sf
Sf
Sf
Sf
Sf
n.p.
n.p.
n.p.
n.p.
I
n.p.
n.p.
n.p.
n.p.
I
3%
3%
3%
3%
30%
30%
30%
30%
10%
10%
10%
10%
5%
5%
5%
5%
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
RG
RG
RG
RG
RG
RG
RG
RG
98%
98%
98%
98%
98%
98%
98%
98%
98%
98%
98%
98%
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Fisher
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
Sigma
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
-
solid
n.p.
undiluted
n.p.
n.p.
paste
solid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
liquid
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
undiluted
undiluted
14.5%
50%
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
1
Source
Lab
No.
Fluorescein Corneal
Retention Opacity
Corneal
Swelling
In Vitro
Classif.
(GHS)1,2
2.4
2.3
1.5
1.7
3
3
*
3
1.4
1
2.67
1.7
1
1.3
2
1
1
0.2
2.5
1
0
2.7
0.6
1.5
0
0
0.8
0.1
1.3
0
1.2
2
2.5
2
4
4
4
4
0.1
0.8
1.17
1
0.6
0
0
2
1
0
1
0.5
0
2
0.3
1.5
0
0
0
0
0.5
0
13.2
38
27.88
26.4
32
153
*
*
9.9
29
20.2
11.2
9.8
38
3.97
39.6
3.6
31
5.63
6.7
0
24
3
9
0
1
2
3
5
1
2A/2B
2A
2A
2A
1
1
*
1
2B
2A/2B
2A
2B
2B
2A
2B
2A
2B
2B
2B
2B
NI
2A
NI
2B
NI
NI
NI
NI
NI
NI
-
2
1.3
19
-
2.5
0.7
0.3
0
0
0.3
0
0
0.5
0.3
0.2
0.2
0.2
0
2
0
0
0
0
0
0
0
0.3
0.3
0.3
0
0
0
35
1
2
2
1
0
0
0
3
0
0
2
3
1
Overall
In Vitro
Classif.
(GHS)
In Vitro
Classif.
(EPA)3,4
NI
2A
NI
2B
NI
NI
NI
NI
NI
NI
II/III
II
II
II
I
I
*
I
III
II/III
II
III
III
II
III
II
III
III
III
III
IV
II
IV
III
IV
IV
IV
IV
IV
IV
2A
2A
2A
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
2A
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
2A
1
2A/2B
2A
2B
Overall
In Vitro
Classif.
(EPA)
In Vitro
Classif.
(EU)5,6
IV
II
IV
III
IV
IV
IV
IV
IV
IV
R36
R36
R36
R36
R41
R41
*
R41
NI
R36
R36
R36
NI
R36
NI
R36
NI
NI/R36
R36
NI
NI
R36
NI
NI
NI
NI
NI
NI
NI
NI
II
II
II
IV
IV
IV
IV
IV
IV
IV
IV
IV
IV
IV
IV
IV
II
IV
IV
IV
IV
IV
IV
IV
IV
IV
IV
IV
IV
IV
II
I
II/III
II
III
Overall
In Vitro
Classif.
(EU)
Reference
NI
R36
NI
NI
NI
NI
NI
NI
NI
NI
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Balls et al. (1995)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
R36
R36
Prinsen (1996)
R36
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
R36
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
R36
R41
R36
R36
NI
In Vitro Data for Substances Tested in the ICE Test Method: Sorted by Reference
Substance/Product Name
TNO-26 (Ink-3)
TNO-27 (Thermal paper coating1)
TNO-28 (Toilet cleaner-1)
TNO-29 (Toilet cleaner-2)
TNO-30 (Pesticide-3)
TNO-31 (Sulfur)
TNO-32 (Ink-4)
TNO-33 (Thermal paper coating2)
TNO-34 (Detergent-2)
TNO-35 (Propyl-lactate)
TNO-36 (Ethylhexyl lactate)
TNO-37 (Pesticide-4)
TNO-38 (Solvent-10)
TNO-39 (Detergent-3)
TNO-40 (Glycolbromoacetate
form.)
TNO-41 (Amidosulfonic acid)
TNO-42 (Glycolbromoacetate)
TNO-43 (Monobromoacetic acid)
TNO-44
(Didecyldimethylammoniumchlor
ide (23% in propyl glycol))
Cetylpyridinium bromide (6%)
Cetylpyridinium bromide (6%)
Cetylpyridinium bromide (6%)
Cetylpyridinium bromide (6%)
Cyclohexylamino-functional PMS
Cyclohexylamino-functional PMS
Cyclohexylamino-functional PMS
Cyclohexylamino-functional PMS
Cyclohexylamino-functional PMS
Decamethylcyclopentasiloxane
Decamethylcyclopentasiloxane
Decamethylcyclopentasiloxane
Decamethylcyclopentasiloxane
Decamethylcyclopentasiloxane
Water
Form
Tested
Solubility
n.p.
liquid
n.p.
liquid
n.p.
n.p.
n.p.
7704-34-9
n.p.
NI
2B
2B
2B
NI
2B
5
25
45
18
15
3
4
n.p.
undiluted
n.p.
n.p.
-
0.1
0
0
n.p.
undiluted
n.p.
n.p.
-
1
0.6
9
liquid
liquid
solid
solid
liquid
n.p.
n.p.
n.p.
I
n.p.
undiluted
undiluted
undiluted
undiluted
undiluted
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
-
1.4
1.3
1.5
0.2
1
0.8
1
1
0
0.5
12
11
7
1
7
n.p.
liquid
n.p.
undiluted
n.p.
n.p.
-
2
0.5
n.p.
616-09-1
6283-86-9
n.p.
n.p.
n.p.
liquid
liquid
liquid
solid
liquid
liquid
n.p.
S
n.p.
n.p.
n.p.
n.p.
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
-
1
3
2
1.5
0
0.5
1
3
2
1
0
0.5
Source
Fluorescein Corneal
Retention Opacity
2B
Purity
1
Lab
No.
Overall
In Vitro
Classif.
(GHS)
Concentration
Tested
CASRN
Corneal
Swelling
In Vitro
Classif.
(EPA)3,4
Overall
In Vitro
Classif.
(EPA)
NI
IV
2B
III
2B
2B
2B
NI
2B
2B
1
1
2A
2B
NI
NI
In Vitro
Classif.
(GHS)1,2
Reference
(EU)5,6
Overall
In Vitro
Classif.
(EU)
IV
NI
NI
Prinsen (1996)
III
NI
NI
Prinsen (1996)
III
III
III
IV
III
III
III
III
IV
III
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
2B
III
III
NI
NI
Prinsen (1996)
1
1
2A
2B
NI
NI
I
I
II
III
IV
IV
I
I
II
III
IV
IV
R41
R41
R36
NI
NI
NI
R41
R41
R36
NI
NI
NI
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
In Vitro
Classif.
n.p.
liquid
n.p.
undiluted
n.p.
n.p.
-
2.6
1.9
41
1
1
I
I
R41
R41
Prinsen (1996)
5329-14-6
3785-34-0
79-08-3
solid
liquid
solid
n.p.
n.p.
S
undiluted
85%
undiluted
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
-
2.7
3
3
4
3
4
46
36
80
1
1
1
1
1
1
I
I
I
I
I
I
R41
R41
R41
R41
R41
R41
Prinsen (1996)
Prinsen (1996)
Prinsen (1996)
7173-51-5
liquid
n.p.
23%
n.p.
n.p.
-
3
3.5
39
1
1
I
I
R41
R41
Prinsen (1996)














liquid
Sf
undiluted
n.p.
n.p.
1
2.0
2.0
22
1
I
R41
liquid
Sf
undiluted
n.p.
n.p.
1
1.8
1.7
21
1
I
R41
liquid
Sf
undiluted
n.p.
n.p.
1
2.0
2.0
21
1
liquid
Sf
undiluted
n.p.
n.p.
1
1.7
1.7
18
1
I
R41
Prinsen (2000)
liquid
n.p.
undiluted
n.p.
n.p.
1
1.8
2.5
14
2A
II
R36
Prinsen (2000)
liquid
n.p.
undiluted
n.p.
n.p.
1
1.7
2.0
13
2A
II
R36
Prinsen (2000)
liquid
n.p.
undiluted
n.p.
n.p.
1
2.0
2.3
17
2A
liquid
n.p.
undiluted
n.p.
n.p.
1
2.0
2.3
14
2A
liquid
n.p.
undiluted
n.p.
n.p.
1
2.0
2.0
13
liquid
n.p.
undiluted
n.p.
n.p.
1
0.3
0.3
1
liquid
n.p.
undiluted
n.p.
n.p.
1
0.3
0.3
liquid
n.p.
undiluted
n.p.
n.p.
1
0.0
liquid
n.p.
undiluted
n.p.
n.p.
1
liquid
n.p.
undiluted
n.p.
n.p.
1
1
2A
I
II
I
II
R41
R36
Prinsen (2000)
R41
R36
Prinsen (2000)
Prinsen (2000)
Prinsen (2000)
II
R36
Prinsen (2000)
2A
II
R36
Prinsen (2000)
NI
NI
NI
Prinsen (2000)
1
NI
NI
NI
0.5
2
NI
0.0
0.0
0
NI
NI
NI
Prinsen (2000)
0.0
0.0
2
NI
NI
NI
Prinsen (2000)
NI
NI
NI
NI
Prinsen (2000)
NI
Prinsen (2000)
In Vitro Data for Substances Tested in the ICE Test Method: Sorted by Reference
Substance/Product Name
Triton X-500 (5%)
Triton X-500 (5%)
Triton X-500 (5%)
Triton X-500 (5%)
Triton X-500 (5%)
TNO-45
TNO-46
TNO-47
TNO-48
TNO-49
TNO-50
TNO-51
TNO-52
TNO-53
TNO-54
TNO-55
TNO-56
TNO-57
TNO-58
TNO-59
TNO-60
TNO-61
TNO-62
TNO-63
TNO-64
TNO-65
TNO-66
TNO-67
TNO-68
TNO-69
TNO-70
TNO-71
TNO-72
TNO-73
TNO-74
TNO-75
TNO-76
TNO-77
TNO-78
TNO-79
TNO-80
TNO-81
TNO-82
TNO-83
TNO-84
CASRN





n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
Water
Form
Tested
Solubility
liquid
liquid
liquid
liquid
liquid
liquid
solid
solid
liquid
liquid
liquid
liquid
solid
liquid
liquid
liquid
solid
solid
liquid
liquid
liquid
solid
emulsion
emulsion
solid
liquid
liquid
liquid
emulsion
emulsion
emulsion
emulsion
solid
liquid
solid
liquid
liquid
solid
solid
liquid
n.p.
n.p.
n.p.
Fluorescein Corneal
Retention Opacity
Corneal
Swelling
Overall
In Vitro
Classif.
(GHS)
In Vitro
Classif.
Overall
In Vitro
Classif.
(EPA)
In Vitro
Classif.
Purity
Sf
undiluted
n.p.
n.p.
1
1.0
0.7
14
2B
III
NI
Sf
undiluted
n.p.
n.p.
1
1.0
0.7
14
2B
III
NI
Sf
Sf
Sf
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
50%
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
undiluted
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
n.p.
1
1
1
-
1.0
1.0
1.0
1
0
0
3
3
3
3
1.7
0.5
1
1.7
2
1.5
0
0.2
0.2
0
2
0.3
1
0.7
0
1
1/2
1
2
1
1.5
2.7
0.5
0
0
1
1.3
1
0.3
0
0
0.8
0.7
0.7
0.8
0.7
0.5
0
0
1
4
3
3
1
0.2
1
1.3
1.3
1.3
0
0
0.5
0
1
0.5
1
0.5
0
1
1
0
1
0.5
0.5
2
0
0
0
0.5
1
1
0
0
0
0.7
0.7
13
8
11
5
1
1
25
41.1
33.9
5
3
9
10
10
12
-1
-2
1
1
12
3
5
4
0
6
8
0
20
13
5
18
0
2
2
7
15
10
-1
1
-2
10
2
2B
2B
2B
NI
NI
NI
2A
1
1
1
2B
NI
2B
2B
2B
2B
NI
NI
NI
NI
2B
NI
2B
NI
NI
2B
2A
NI
2A
2B
NI
1
NI
NI
NI
2B
2B
2B
NI
NI
NI
2B
2B
Source
Lab
No.
In Vitro
Classif.
Concentration
Tested
1
(GHS)1,2
2B
NI
NI
NI
2A
1
1
1
2B
NI
2B
2B
2B
2B
NI
NI
NI
NI
2B
NI
2B
NI
NI
2B
2A
NI
2A
2B
NI
1
NI
NI
NI
2B
2B
2B
NI
NI
NI
2B
2B
(EPA)3,4
III
III
III
NI
NI
NI
II
I
I
I
III
NI
III
III
III
III
NI
NI
NI
NI
III
IV
III
NI
NI
III
II
NI
II
III
NI
I
IV
NI
NI
III
III
III
NI
NI
NI
III
III
III
NI
NI
NI
II
I
I
I
III
NI
III
III
III
III
NI
NI
NI
NI
III
IV
III
NI
NI
III
II
NI
II
III
NI
I
IV
NI
NI
III
III
III
NI
NI
NI
III
III
(EU)5,6
NI
NI
NI
NI
NI
NI
R36
R41
R41
R41
NI
NI
NI
R36
R36
NI
NI
NI
NI
NI
R36
NI
NI
NI
NI
NI
R36
NI
R36
NI
NI
R41
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
Overall
In Vitro
Classif.
(EU)
Reference
Prinsen (2000)
Prinsen (2000)
NI
NI
NI
NI
R36
R41
R41
R41
NI
NI
NI
R36
R36
NI
NI
NI
NI
NI
R36
NI
NI
NI
NI
NI
R36
NI
R36
NI
NI
R41
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
NI
Prinsen (2000)
Prinsen (2000)
Prinsen (2000)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
In Vitro Data for Substances Tested in the ICE Test Method: Sorted by Reference
Substance/Product Name
CASRN
Form
Tested
Water
Solubility
1
Concentration
Tested
Purity
Source
Lab
No.
Fluorescein Corneal
Retention Opacity
TNO-85
n.p.
n.p.
n.p.
undiluted
n.p.
n.p.
2
TNO-86
n.p.
n.p.
n.p.
undiluted
n.p.
n.p.
1
TNO-87
n.p.
liquid
n.p.
undiluted
n.p.
n.p.
0.7
TNO-88
n.p.
liquid
n.p.
undiluted
n.p.
n.p.
0.3
TNO-89
n.p.
solid
n.p.
undiluted
n.p.
n.p.
0.2
TNO-90
n.p.
liquid
n.p.
undiluted
n.p.
n.p.
0
TNO-91
n.p.
liquid
n.p.
undiluted
n.p.
n.p.
0
TNO-92
n.p.
solid
n.p.
undiluted
n.p.
n.p.
0.8
TNO-93
n.p.
emulsion
n.p.
undiluted
n.p.
n.p.
3
TNO-94
n.p.
liquid
n.p.
undiluted
n.p.
n.p.
1
1-Butanol
71-36-3
liquid
S
undiluted
99%
Aldrich
2.9
2-Butoxyethyl acetate
112-07-2
liquid
S
undiluted
99%
Aldrich
1
2-Methoxyethanol
109-86-4
liquid
S
undiluted
99.9%
Aldrich
2
Acetaldehyde
75-07-0
liquid
S
undiluted
99%
Aldrich
2
Acetic acid
64-19-7
liquid
S
10%
99%
Aldrich
3
Benzalkonium chloride (100%)
8001-54-5
liquid
S
undiluted
n.p.
Aldrich
3
Brij 35
9002-92-0
liquid
S
undiluted
n.p.
Aldrich
0.9
Chloroform
67-66-3
liquid
I*
undiluted
99.8%
Aldrich
2.5
Dibutyltin dichloride
683-18-1
solid
S
undiluted
97%
Aldrich
3
Dimethyl sulfoxide
67-68-5
liquid
S
undiluted
99.9%
Aldrich
1
Glycerol
56-81-5
liquid
S
undiluted
99%
Aldrich
0.5
Mercury (II) chloride
7487-94-7
solid
I
undiluted
99.5%
Aldrich
2
n-Hexane
110-54-3
liquid
I
undiluted
99%
Aldrich
0.5
Silver (I) nitrate
7761-88-8
solid
S
3%
99.5%
Aldrich
1
Sodium dodecyl sulfate
151-21-3
solid
S
undiluted
70%
Aldrich
0.8
Sodium fluorescein
518-47-8
liquid
S
20%
Aldrich
0.1
70%8
Sodium hydroxide
1310-73-2
liquid
S
1%
97%
Aldrich
3
Toluene
108-88-3
liquid
I*
undiluted
99.9%
Aldrich
1.1
Triacetin
102-76-1
liquid
I*
undiluted
99%
Aldrich
0.5
Tributyltin chloride
1461-22-9
liquid
n.p.
undiluted
96%
Aldrich
3
Triethanolamine
102-71-6
liquid
S
undiluted
99%
Aldrich
0.9
Abbreviations: S = soluble; Sf = surfactant; I = insoluble; *solubility uncertain; RG = reagent grade; n.p. = not provided and not obtained
1
1.3
1
1
0.7
0.7
0
0.2
1.7
2
0.5
2
1
2
1.4
2.6
3
0
1
2.5
0.5
0.4
3.1
0
1
1
0
3
1.4
0.4
2.5
0.7
Corneal
Swelling
14
7
1
3
1
2
1
16
17
2
54
5
18
24
31
40
5
21
34
4
4
55
1
12
22
0
60
4
4
48
4
(GHS)1,2
Overall
In Vitro
Classif.
(GHS)
2B
2B
2B
NI
NI
NI
NI
2B
2A
NI
1
2B
2A
2A
1
1
NI
2A
1
NI
NI
1
NI
2B
2B
NI
1
2B
NI
1
2B
2B
2B
2B
NI
NI
NI
NI
2B
2A
NI
1
2B
2A
2A
1
1
NI
2A
1
NI
NI
1
NI
2B
2B
NI
1
2B
NI
1
2B
In Vitro
Classif.
In Vitro
Classif.
(EPA)3,4
Overall
In Vitro
Classif.
(EPA)
III
III
III
NI
NI
NI
NI
III
II
NI
I
III
II
II
I
I
IV
II
I
IV
IV
I
IV
III
III
IV
I
III
IV
I
III
III
III
III
NI
NI
NI
NI
III
II
NI
I
III
II
II
I
I
IV
II
I
IV
IV
I
IV
III
III
IV
I
III
IV
I
III
In Vitro
Classif.
Reference
(EU)5,6
Overall
In Vitro
Classif.
(EU)
R36
NI
NI
NI
NI
NI
NI
R36
R36
NI
R41
NI
R36
R36
R41
R41
NI
R36
R41
NI
NI
R41
NI
NI
R41
NI
R41
NI
NI
R41
NI
R36
NI
NI
NI
NI
NI
NI
R36
R36
NI
R41
NI
R36
R36
R41
R41
NI
R36
R41
NI
NI
R41
NI
NI
R41
NI
R41
NI
NI
R41
NI
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen (2005)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
Prinsen and Koëter (1993)
GHS=Globally Harmonized System (UN 2007)
Eye Irritant Category 1 = irreversible effects on the eye/serious damage to the eye; Category 2A = reversible effects on the eye/irritating to the eyes; Category 2B = reversible effects on the eye/mildly irritating to the eyes; Nonirritant = not an eye irritant.
EPA=U.S. Environmental Protection Agency (EPA 2003a).
4
Toxicity Category I for the Primary Eye Irritation Study = Corrosive, or corneal involvement or irritation not reversible within 21 days; Category II = Corneal involvement or irritation clearing in 8-21 days; Category III = Corneal involvement or irritation clearing in 1-7 days; Category IV: minimal effects clearing in less than 24 hr.
2
3
5
EU=European Union (EU 2001).
6
Risk phrase R41 = risk of serious damage to the eyes; R36 = irritating to the eyes; nonirritant = not an eye irritant.
7
Numbering for substances from this reference assigned based on order of appearance in Table 3 of Prinsen (1996) 8
Dye content