Broken Beaker Science Express Grade Levels 7-12

Science Express
Broken Beaker
Grade Levels
7-12
Objectives
To understand the use of Restriction Enzymes and how they cut DNA.
Review or learn the structure of DNA.
To use Electrophoresis to determine which DNA was found at a mock crime
scene.
To understand how bars are formed in Electrophoresis (based on fragment size).
Learn to function and properly use micropipettes, centrifuge, and vortex mixer.
Vocabulary
DNA
Nitrogen Bases (A -T, G -C)
Pipette
Micro-liter
Recognition Site
Centrifuge
Restriction Enzymes
Electrophoresis
Fragments (DNA)
Polymerase
Vortex mixer
Pre-Lab Recommendations, Not Required
1. DNA Structure: Double stranded, Nitrogenous bases (A –T, G –C)
2. DNA Sequencing
3. DNA use in criminal investigation